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In farmed flatfish, such as common sole, color disturbances are common. Dyschromia is a general term that includes the color defects on the blind and ocular sides of the fish. The purpose was to examine the difference in gene expression between normal pigmented and juveniles who present ambicoloration. The analysis was carried out with next-generation sequencing techniques and de novo assembly of the transcriptome. Transcripts that showed significant differences (FDR < 0.05) in the expression between the two groups, were related to those of zebrafish (Danio rerio), functionally identified, and classified into categories of the gene ontology. The results revealed that ambicolorated juveniles exhibit a divergent function, mainly of the central nervous system at the synaptic level, as well as the ionic channels. The close association of chromophore cells with the growth of nerve cells and the nervous system was recorded. The pathway, glutamate binding–activation of AMPA and NMDA receptors–long-term stimulation of postsynaptic potential–LTP (long term potentiation)–plasticity of synapses, appears to be affected. In addition, the development of synapses also seems to be affected by the interaction of the LGI (leucine-rich glioma inactivated) protein family with the ADAM (a disintegrin and metalloprotease) ones.
Menelaos Kavouras; Emmanouil Malandrakis; Ewout Blom; Kyriaki Tsilika; Theodoros Danis; Panagiota Panagiotaki; Athanasios Exadactylos. Malpigmentation of Common Sole (Solea solea) during Metamorphosis Is Associated with Differential Synaptic-Related Gene Expression. Animals 2021, 11, 2273 .
AMA StyleMenelaos Kavouras, Emmanouil Malandrakis, Ewout Blom, Kyriaki Tsilika, Theodoros Danis, Panagiota Panagiotaki, Athanasios Exadactylos. Malpigmentation of Common Sole (Solea solea) during Metamorphosis Is Associated with Differential Synaptic-Related Gene Expression. Animals. 2021; 11 (8):2273.
Chicago/Turabian StyleMenelaos Kavouras; Emmanouil Malandrakis; Ewout Blom; Kyriaki Tsilika; Theodoros Danis; Panagiota Panagiotaki; Athanasios Exadactylos. 2021. "Malpigmentation of Common Sole (Solea solea) during Metamorphosis Is Associated with Differential Synaptic-Related Gene Expression." Animals 11, no. 8: 2273.
Stuckenia pectinata, a submerged macrophyte of eutrophic to hyper-eutrophic fresh to brackish waters, faces management and climatic-forced increment of salinity and irradiance in Vistonis Lake (Greece) that may endanger its existence and the ecosystem functioning. A pre-acclimated clone under low irradiance and salinity conditions was treated to understand the effects of high salinity and irradiance on a suite of subcellular (chlorophyll a fluorescence kinetics and JIP-test, and chlorophyll content) to organismal (relative growth rate—RGR) physiological parameters. The responses to high irradiance indicated the plant’s great photo-acclimation potential to regulate the number and size of the reaction centers and the photosynthetic electron transport chain by dissipation of the excess energy to heat. A statistically significant interaction (p< 0.01) of salinity and irradiance on Chl a, b content indicated acclimation potential through adjusting the Chl a, b contents. However, no significant (p > 0.05) difference was observed on Chl a/b ratio and the RGR, indicating the species’ potential to become acclimatized by reallocating resources to compensate for growth. Thus, the regulation of photosynthetic pigment content and photosystem II performance consisted of the primary growth strategy to present and future high salinity and irradiance stressful conditions due to eutrophication management and the ongoing climatic changes.
Lamprini Malea; Konstantinia Nakou; Apostolos Papadimitriou; Athanasios Exadactylos; Sotiris Orfanidis. Physiological Responses of the Submerged Macrophyte Stuckenia pectinata to High Salinity and Irradiance Stress to Assess Eutrophication Management and Climatic Effects: An Integrative Approach. Water 2021, 13, 1706 .
AMA StyleLamprini Malea, Konstantinia Nakou, Apostolos Papadimitriou, Athanasios Exadactylos, Sotiris Orfanidis. Physiological Responses of the Submerged Macrophyte Stuckenia pectinata to High Salinity and Irradiance Stress to Assess Eutrophication Management and Climatic Effects: An Integrative Approach. Water. 2021; 13 (12):1706.
Chicago/Turabian StyleLamprini Malea; Konstantinia Nakou; Apostolos Papadimitriou; Athanasios Exadactylos; Sotiris Orfanidis. 2021. "Physiological Responses of the Submerged Macrophyte Stuckenia pectinata to High Salinity and Irradiance Stress to Assess Eutrophication Management and Climatic Effects: An Integrative Approach." Water 13, no. 12: 1706.
Existing literature on dolphin-fisheries interaction focused on Greece reveals both an undeveloped area for research, but also a lack of relevant data in this field. Although imperative, relevant research has been slow on innovation and cooperation among universities, official bureaus, and NGOs that are obliged to work together as European and national laws dictate. Most of the research in this new field focuses on the interaction between marine mammals and local fisheries, suggesting that this relationship may be problematic for both parties since the former are being treated (at least occasionally) with brutality, while the latter try to deal with economic loss. Dolphins and fishermen operate within the same ecological niches for their survival, the main area of conflict being nutritious fish. Anthropological research on ethnic identity has long dealt with antagonistic relationships over resources between adjacent groups of people. Marine biologists’ research in Greece focuses on the human factor, and some of its shortcomings may well be seen as the result of limited, or an absence of, training in social sciences. This article attempts to draw from anthropological theory to shed light on a particular symbiosis between humans and dolphins. Multidisciplinary approaches gain ground in a wide range of research interests and seem to be fruitful in terms of theoretical and practical results.
Stergios Pardalis; Anastasia Komnenou; Athanasios Exadactylos; Georgios Gkafas. Small Scale Fisheries, Dolphins and Societal Challenges: A Case Study in the City of Volos, Greece. Conservation 2021, 1, 81 -90.
AMA StyleStergios Pardalis, Anastasia Komnenou, Athanasios Exadactylos, Georgios Gkafas. Small Scale Fisheries, Dolphins and Societal Challenges: A Case Study in the City of Volos, Greece. Conservation. 2021; 1 (2):81-90.
Chicago/Turabian StyleStergios Pardalis; Anastasia Komnenou; Athanasios Exadactylos; Georgios Gkafas. 2021. "Small Scale Fisheries, Dolphins and Societal Challenges: A Case Study in the City of Volos, Greece." Conservation 1, no. 2: 81-90.
The verification of authenticity and detection of food mislabeling are elements that have been of high importance for centuries. During the last few decades there has been an increasing consumer demand for the verification of food identity and the implementation of stricter controls around these matters. Fish and seafood are among the most easily adulterated foodstuffs mainly due to the significant alterations of the species' morphological characteristics that occur during the different types of processing, which render the visual identification of the animals impossible. Even simple processes, such as filleting remove very important morphological elements and suffice to prevent the visual identification of species in marketed products. Novel techniques have therefore been developed that allow identification of species, the differentiation between species and also the differentiation of individuals that belong to the same species but grow in different populations and regions. Molecular markers have been used during the last few decades to fulfill this purpose and several improvements have been implemented rendering their use applicable to a commercial scale. The reliability, accuracy, reproducibility, and time‐and cost‐effectiveness of these techniques allowed them to be established as routine methods in the industry and research institutes. This review article aims at presenting the most important molecular markers used for the authentication of fish and seafood. The most important techniques are described, and the results of numerous studies are outlined and discussed, allowing interested parties to easily access and compare information about several techniques and fish/seafood species.
Konstantinos V. Kotsanopoulos; Athanasios Exadactylos; George A. Gkafas; Petros V. Martsikalis; Foteini F. Parlapani; Ioannis S. Boziaris; Ioannis S. Arvanitoyannis. The use of molecular markers in the verification of fish and seafood authenticity and the detection of adulteration. Comprehensive Reviews in Food Science and Food Safety 2021, 20, 1584 -1654.
AMA StyleKonstantinos V. Kotsanopoulos, Athanasios Exadactylos, George A. Gkafas, Petros V. Martsikalis, Foteini F. Parlapani, Ioannis S. Boziaris, Ioannis S. Arvanitoyannis. The use of molecular markers in the verification of fish and seafood authenticity and the detection of adulteration. Comprehensive Reviews in Food Science and Food Safety. 2021; 20 (2):1584-1654.
Chicago/Turabian StyleKonstantinos V. Kotsanopoulos; Athanasios Exadactylos; George A. Gkafas; Petros V. Martsikalis; Foteini F. Parlapani; Ioannis S. Boziaris; Ioannis S. Arvanitoyannis. 2021. "The use of molecular markers in the verification of fish and seafood authenticity and the detection of adulteration." Comprehensive Reviews in Food Science and Food Safety 20, no. 2: 1584-1654.
Thodoros Kampouris; Georgios Gkafas; Joanne Sarantopoulou; Athanasios Exadactylos; Ioannis Batjakas. An American in the Aegean: first record of the American lobster Homarus americanus H. Milne Edwards, 1837 from the eastern Mediterranean Sea. BioInvasions Records 2021, 10, 170 -180.
AMA StyleThodoros Kampouris, Georgios Gkafas, Joanne Sarantopoulou, Athanasios Exadactylos, Ioannis Batjakas. An American in the Aegean: first record of the American lobster Homarus americanus H. Milne Edwards, 1837 from the eastern Mediterranean Sea. BioInvasions Records. 2021; 10 (1):170-180.
Chicago/Turabian StyleThodoros Kampouris; Georgios Gkafas; Joanne Sarantopoulou; Athanasios Exadactylos; Ioannis Batjakas. 2021. "An American in the Aegean: first record of the American lobster Homarus americanus H. Milne Edwards, 1837 from the eastern Mediterranean Sea." BioInvasions Records 10, no. 1: 170-180.
The Aegean Sea is an insular archipelago in North Mediterranean Sea. It exhibits 60 inhabited islands, more than 1,400 small islands and more than 2,500 outcrops and rocks. Its total area is in excess of 215,000 km2. Fisheries and aquaculture are the second most important economic activities after tourism, from the point of view of contribution to the national GDP. However, as segments of the primary production, they are more important than tourism for job and income security of the numerous rural fishery-dependent communities in the region, as tourism activity is limited in certain areas and islands. The fishing fleet in the Aegean Sea amounts to 11,580 vessels approximately from which 95% belongs to the small-scale fishery segment and operates from over 100 ports, harbours and fishing refuges on the mainland and the islands. Fisheries production reached almost 69,000 t in 2018. Aquaculture in Greece is a fast developing industry with export orientation. The total national aquaculture production today amounts to 133,000 t of sea bass and sea bream for 2016, compared to 400 t in 1984. Aquaculture production in the Aegean Sea amounts to 83,000 t (63% of total) and fetching approximately 370 million €. From the economic point of view, the fisheries sector in the Aegean Sea faces many challenges. Even though demand for high-quality fishery products is stable and increasing, mainly due to tourism, the market structure is such that net income margins for capture fisheries are low between 8 and 12% at the producer level. Aquaculture suffers from very low prices close or seasonally below production cost levels due to the market competition of aquaculture production of the same species (whole, fresh or chilled sea bass and sea bream) by most Mediterranean States. Finally, fishery product processing industry faces drops in the demand of traditional products in the region (canned in oil, smoked) and low market prices for other types of products (fillets, frozen, etc.) which has forced many companies to diverse their production to vegetables and other local high-market value produce.
Alexis Conides; Dimitris Klaoudatos; Miltiadis Kalamaras; Nikolaos Neophytou; Athanasios Exadactylos; Costas Papaconstantinou; Spyros Klaoudatos. Capture Fisheries and Aquaculture Exploitation in the Aegean Sea Archipelago. The Handbook of Environmental Chemistry 2020, 1 -26.
AMA StyleAlexis Conides, Dimitris Klaoudatos, Miltiadis Kalamaras, Nikolaos Neophytou, Athanasios Exadactylos, Costas Papaconstantinou, Spyros Klaoudatos. Capture Fisheries and Aquaculture Exploitation in the Aegean Sea Archipelago. The Handbook of Environmental Chemistry. 2020; ():1-26.
Chicago/Turabian StyleAlexis Conides; Dimitris Klaoudatos; Miltiadis Kalamaras; Nikolaos Neophytou; Athanasios Exadactylos; Costas Papaconstantinou; Spyros Klaoudatos. 2020. "Capture Fisheries and Aquaculture Exploitation in the Aegean Sea Archipelago." The Handbook of Environmental Chemistry , no. : 1-26.
Long-term exposure of Mytilus galloprovincialis to temperatures beyond 26°C triggers mussel mortality. The present study aimed to integratively illustrate the correlation between intermediary metabolism, hsp gene expression, and oxidative stress-related proteins in long-term thermally stressed Mytilus galloprovincialis and whether they are affected by thermal stress magnitude and duration. We accordingly evaluated the gene expression profiles, in the posterior adductor muscle (PAM) and the mantle, concerning heat shock protein 70 and 90 ( hsp70 and hsp90), and the antioxidant defense indicators Mn-SOD, Cu/Zn-SOD, catalase, glutathione S-transferase, and the metallothioneins mt-10 and mt-20. Moreover, we determined antioxidant enzyme activities, oxidative stress through lipid peroxidation, and activities of intermediary metabolism enzymes. The pattern of changes in relative mRNA expression levels indicate that mussels are able to sense thermal stress even when exposed to 22°C and before mussel mortality is initiated. Data indicate a close correlation between the magnitude and duration of thermal stress with lipid peroxidation levels and changes in the activity of antioxidant enzymes and the enzymes of intermediary metabolism. The gene expression and increase in the activities of antioxidant enzymes support a scenario, according to which exposure to 24°C might trigger reactive oxygen species (ROS) production, which is closely correlated with anaerobic metabolism under hypometabolic conditions. Increase and maintenance of oxidative stress in conjunction with energy balance disturbance seem to trigger mussel mortality after long-term exposure at temperatures beyond 26°C. Eventually, in the context of preparation for oxidative stress, certain hypotheses and models are suggested, integrating the several steps of cellular stress response.
Konstantinos Feidantsis; Ioannis A. Giantsis; Andreas Vratsistas; Stavroula Makri; Athanasia-Zoi Pappa; Elena Drosopoulou; Andreas Anestis; Evangelia Mavridou; Athanasios Exadactylos; Dimitrios Vafidis; Basile Michaelidis. Correlation between intermediary metabolism, Hsp gene expression, and oxidative stress-related proteins in long-term thermal-stressed Mytilus galloprovincialis. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 2020, 319, R264 -R281.
AMA StyleKonstantinos Feidantsis, Ioannis A. Giantsis, Andreas Vratsistas, Stavroula Makri, Athanasia-Zoi Pappa, Elena Drosopoulou, Andreas Anestis, Evangelia Mavridou, Athanasios Exadactylos, Dimitrios Vafidis, Basile Michaelidis. Correlation between intermediary metabolism, Hsp gene expression, and oxidative stress-related proteins in long-term thermal-stressed Mytilus galloprovincialis. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 2020; 319 (3):R264-R281.
Chicago/Turabian StyleKonstantinos Feidantsis; Ioannis A. Giantsis; Andreas Vratsistas; Stavroula Makri; Athanasia-Zoi Pappa; Elena Drosopoulou; Andreas Anestis; Evangelia Mavridou; Athanasios Exadactylos; Dimitrios Vafidis; Basile Michaelidis. 2020. "Correlation between intermediary metabolism, Hsp gene expression, and oxidative stress-related proteins in long-term thermal-stressed Mytilus galloprovincialis." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 319, no. 3: R264-R281.
The impact of inbreeding on fitness has been widely studied and provides consequential inference about adaptive potential and the impact on survival for reduced and fragmented natural populations. Correlations between heterozygosity and fitness are common in the literature, but they rarely inform about the likely mechanisms. Here, we investigate a pathology with a clear impact on health in striped dolphin hosts (a nematode infection that compromises lung function). Dolphins varied with respect to their parasite burden of this highly pathogenic lung nematode ( Skrjabinalius guevarai ). Genetic diversity revealed by high-resolution restriction-associated DNA (43 018 RADseq single nucleotide polymorphisms) analyses showed a clear association between heterozygosity and pathogen load, but only for female dolphins, for which the more heterozygous individuals had lower Sk. guevarai burden. One locus identified by RADseq was a strong outlier in association with parasite load (heterozygous in all uninfected females, homozygous for 94% of infected females), found in an intron of the citron rho-interacting serine/threonine kinase locus (associated with milk production in mammals). Allelic variation at the Class II major histocompatability complex DQB locus was also assessed and found to be associated with both regional variation and with pathogen load. Both sex specificity and the identification of associating functional loci provide insight into the mechanisms by which more inbred individuals may be more susceptible to the infection of this parasite. This provides important insight towards our understanding of the impact of inbreeding in natural populations, relevant to both evolutionary and practical conservation considerations.
Georgios A. Gkafas; Menno de Jong; Athanasios Exadactylos; Juan Antonio Raga; Francisco Javier Aznar; A. Rus Hoelzel. Sex-specific impact of inbreeding on pathogen load in the striped dolphin. Proceedings of the Royal Society B: Biological Sciences 2020, 287, 20200195 .
AMA StyleGeorgios A. Gkafas, Menno de Jong, Athanasios Exadactylos, Juan Antonio Raga, Francisco Javier Aznar, A. Rus Hoelzel. Sex-specific impact of inbreeding on pathogen load in the striped dolphin. Proceedings of the Royal Society B: Biological Sciences. 2020; 287 (1922):20200195.
Chicago/Turabian StyleGeorgios A. Gkafas; Menno de Jong; Athanasios Exadactylos; Juan Antonio Raga; Francisco Javier Aznar; A. Rus Hoelzel. 2020. "Sex-specific impact of inbreeding on pathogen load in the striped dolphin." Proceedings of the Royal Society B: Biological Sciences 287, no. 1922: 20200195.
The distribution and abundance of three Delphinidae species (striped dolphin, bottlenose dolphin, and common dolphin) were investigated in an extended area of the Mediterranean Sea. Data from nondesigned transect line surveys were modeled in order to investigate important marine areas for marine mammals. The results indicated that the environmental covariates significantly affecting marine mammals’ distribution/abundance were depth and distance from 200 m isobaths for striped dolphin, latitude/longitude and depth for bottlenose dolphin, and latitude/longitude and chlorophyll concentration for common dolphin. Maps of predictions were designed in order to communicate the results of important hot spot areas throughout the Mediterranean. Monitoring of Delphinidae species population patterns in the Mediterranean Sea was carried out in a sequence of surveys employing different approaches. Data from seven-year surveys with small catamaran sailing boats were analyzed under model-based approaches. Density Surface Models were used to produce spatial distribution prediction of three Delphinidae species (Stenella coeruleoalba, Tursiops truncatus, and Delphinus delphis) in an extended study area covering much of the Mediterranean Sea. A classical distance sampling protocol was applied in order to calculate the detection probability of clusters. Static (depth, slope, distance from the coast, and distance from isobaths of 200 m) and nonstatic (sea surface temperature and chlorophyll) variables were used to predict the species distribution/abundance in a generalized additive model context. Stenella coeruleoalba was found to be the dominant species, with an extended distribution in the study area; its abundance was significantly affected by both depth and distance. Tursiops truncatus and Delphinus delphis illustrated a significant abundance correlation with depth and chlorophyll, respectively, while both species showed a robust longitude correlation. Our model pinpoints the significance of nondesigned transect line surveys, suggesting the importance of specific habitat areas for future monitoring and conservation aspects of marine mammals.
Grigorios Karamitros; Georgios A. Gkafas; Ioannis A. Giantsis; Petros Martsikalis; Menelaos Kavouras; Athanasios Exadactylos. Model-Based Distribution and Abundance of Three Delphinidae in the Mediterranean. Animals 2020, 10, 260 .
AMA StyleGrigorios Karamitros, Georgios A. Gkafas, Ioannis A. Giantsis, Petros Martsikalis, Menelaos Kavouras, Athanasios Exadactylos. Model-Based Distribution and Abundance of Three Delphinidae in the Mediterranean. Animals. 2020; 10 (2):260.
Chicago/Turabian StyleGrigorios Karamitros; Georgios A. Gkafas; Ioannis A. Giantsis; Petros Martsikalis; Menelaos Kavouras; Athanasios Exadactylos. 2020. "Model-Based Distribution and Abundance of Three Delphinidae in the Mediterranean." Animals 10, no. 2: 260.
High-resolution melting (HRM) analysis followed by sequencing was applied for determination of bacteria grown on plates isolated from farmed mussels (Mytilus galloprovincialis) during their storage at 4 °C. The V3-V4 region of the 16S rRNA gene from the isolates was amplified using 16S universal primers. Melting curves (peaks) and high resolution melting curves (shape) of the amplicons and sequencing analysis were used for differentiation and identification of the isolated bacteria, respectively. The majority of the isolates (a sum of 101 colonies, from five time intervals: day 0, 2, 4, 6 and 8) from non-selective solid medium plates were classified in four bacterial groups based on the melting curves (peaks) and HRM curves (shape) of the amplicons, while three isolates presented distinct HRM curve profiles (single). Afterwards, sequencing analysis showed that the isolates with a) the same melting peak temperature and b) HRM curves that were >95% similar grouped into the same bacterial species. Therefore, based on this methodology, the cultivable microbial population of chill-stored mussels was initially dominated by Psychrobacter alimentarius against others, such as Psychrobacter pulmonis, Psychrobacter celer and Klebsiella pneumoniae. P. alimentarius was also the dominant microorganism at the time of the sensory rejection (day 8). Concluding, HRM analysis could be used as a useful tool for the rapid differentiation of the bacteria isolated from mussels during storage, at species level, and then identification is feasible by the sequencing of one only representative of each bacterial species, thus reducing the cost of required sequencing.
F.F. Parlapani; F. Syropoulou; A. Tsiartsafis; S. Ekonomou; P. Madesis; A. Exadactylos; I.S. Boziaris. HRM analysis as a tool to facilitate identification of bacteria from mussels during storage at 4 °C. Food Microbiology 2020, 85, 103304 .
AMA StyleF.F. Parlapani, F. Syropoulou, A. Tsiartsafis, S. Ekonomou, P. Madesis, A. Exadactylos, I.S. Boziaris. HRM analysis as a tool to facilitate identification of bacteria from mussels during storage at 4 °C. Food Microbiology. 2020; 85 ():103304.
Chicago/Turabian StyleF.F. Parlapani; F. Syropoulou; A. Tsiartsafis; S. Ekonomou; P. Madesis; A. Exadactylos; I.S. Boziaris. 2020. "HRM analysis as a tool to facilitate identification of bacteria from mussels during storage at 4 °C." Food Microbiology 85, no. : 103304.
Two feeding trials were conducted to examine the replacement of fishmeal (FM) by hydrolyzed feather meal (HFM) in the diet of juvenile gilthead sea bream (Sparus aurata). In trial I (100 days), three isonitrogenous (500 g kg−1 crude protein) and isoenergetic (21 kJ kg−1) diets were formulated, where FM protein was replaced by HFM at 50% (HFM50) and 100% (HFM100), while in trial II (110 days) four diets were formulated using the same FM control diet but FM was replaced at lower levels: 25% (HFM25), and 25% (HFM25+) and 50% (HFM50+) supplemented with lysine and methionine. Juveniles were allocated in quadruplicate and triplicate groups, respectively for trial I and II and fed to satiation 2 times day−1. HFM can successfully replace FM protein at 25% without compromising the feed intake, growth performance, feed utilization and proximate composition of juvenile gilthead seabream. Higher FM replacement levels down-regulated the liver gene expression of the GH/IGF axis and thus reduced the growth performance and nutrient metabolism, although the digestive protease activities and several haematological parameters were unaffected.
P. Psofakis; I.T. Karapanagiotidis; Emmanouil Malandrakis; E. Golomazou; Athanasios Exadactylos; E. Mente. Effect of fishmeal replacement by hydrolyzed feather meal on growth performance, proximate composition, digestive enzyme activity, haematological parameters and growth-related gene expression of gilthead seabream (Sparus aurata). Aquaculture 2020, 521, 735006 .
AMA StyleP. Psofakis, I.T. Karapanagiotidis, Emmanouil Malandrakis, E. Golomazou, Athanasios Exadactylos, E. Mente. Effect of fishmeal replacement by hydrolyzed feather meal on growth performance, proximate composition, digestive enzyme activity, haematological parameters and growth-related gene expression of gilthead seabream (Sparus aurata). Aquaculture. 2020; 521 ():735006.
Chicago/Turabian StyleP. Psofakis; I.T. Karapanagiotidis; Emmanouil Malandrakis; E. Golomazou; Athanasios Exadactylos; E. Mente. 2020. "Effect of fishmeal replacement by hydrolyzed feather meal on growth performance, proximate composition, digestive enzyme activity, haematological parameters and growth-related gene expression of gilthead seabream (Sparus aurata)." Aquaculture 521, no. : 735006.
In sole aquaculture production, consistency in the quality of produced eggs throughout the year is unpredictable. Hox genes have a crucial role in controlling embryonic development and their genetic variation could alter the phenotype dramatically. In teleosts genome duplication led paralog hox genes to become diverged. Direct association of polymorphism in hoxa1a, hoxa2a & hoxa2b of Solea solea with egg viability indicates hoxa2b as a potential genetic marker. High Resolution Melt (HRM) analysis was carried out in 52 viable and 61 non-viable eggs collected at 54±6 hours post fertilization (hpf). Allelic and genotypic frequencies of polymorphism were analyzed and results illustrated a significantly increased risk for non-viability for minor alleles and their homozygous genotypes. Haplotype analysis demonstrated a significant recessive effect on the risk of non-viability, by increasing the odds of disrupting embryonic development up to three-fold. Phylogenetic analysis showed that the paralog genes hoxa2a and hoxa2b, are separated distinctly in two clades and presented a significant ω variation, revealing their diverged evolutionary rate.
Menelaos Kavouras; Emmanouil E. Malandrakis; Theodoros Danis; Ewout Blom; Konstantinos Anastassiadis; Panagiota Panagiotaki; Athanasios Exadactylos. Hox genes polymorphism depicts developmental disruption of common sole eggs. Open Life Sciences 2019, 14, 549 -563.
AMA StyleMenelaos Kavouras, Emmanouil E. Malandrakis, Theodoros Danis, Ewout Blom, Konstantinos Anastassiadis, Panagiota Panagiotaki, Athanasios Exadactylos. Hox genes polymorphism depicts developmental disruption of common sole eggs. Open Life Sciences. 2019; 14 (1):549-563.
Chicago/Turabian StyleMenelaos Kavouras; Emmanouil E. Malandrakis; Theodoros Danis; Ewout Blom; Konstantinos Anastassiadis; Panagiota Panagiotaki; Athanasios Exadactylos. 2019. "Hox genes polymorphism depicts developmental disruption of common sole eggs." Open Life Sciences 14, no. 1: 549-563.
The authors wish to make the following corrections to this paper
Athanasios Exadactylos; Dimitrios Vafidis; Costas S. Tsigenopoulos; Georgios A. Gkafas. Addendum: Exadactylos, A., et al. High Connectivity of the White Seabream (Diplodus sargus, L. 1758) in the Aegean Sea, Eastern Mediterranean Basin. Animals 2019, 11, 979. Animals 2019, 9, 1152 .
AMA StyleAthanasios Exadactylos, Dimitrios Vafidis, Costas S. Tsigenopoulos, Georgios A. Gkafas. Addendum: Exadactylos, A., et al. High Connectivity of the White Seabream (Diplodus sargus, L. 1758) in the Aegean Sea, Eastern Mediterranean Basin. Animals 2019, 11, 979. Animals. 2019; 9 (12):1152.
Chicago/Turabian StyleAthanasios Exadactylos; Dimitrios Vafidis; Costas S. Tsigenopoulos; Georgios A. Gkafas. 2019. "Addendum: Exadactylos, A., et al. High Connectivity of the White Seabream (Diplodus sargus, L. 1758) in the Aegean Sea, Eastern Mediterranean Basin. Animals 2019, 11, 979." Animals 9, no. 12: 1152.
Population dynamics in the marine realm can shape species’ spatial structure and genetic variability between given geographical areas. Connectivity is an important factor of species’ population structure. In this study, we examined the genetic diversity and structure of white seabream (Diplodus sargus, L. 1758) in the eastern Mediterranean basin, using a panel of four microsatellite markers. Recorded low FST values within the study area indicate little evidence of genetic differentiation among populations. Results suggest high gene flow which may imply near-panmixia between populations, indicating the possibility of a probable movement of adult migrants, or strong passive drift at sea in early life stages of the species. To this extent, bibliographically speaking, different species within the Sparidae family favor altered population dynamics patterns with respect to local populations and genetic divergence, in the context of the molecular marker used.
Athanasios Exadactylos; Dimitrios Vafidis; Costas S. Tsigenopoulos; Georgios A. Gkafas. High Connectivity of the White Seabream (Diplodus sargus, L. 1758) in the Aegean Sea, Eastern Mediterranean Basin. Animals 2019, 9, 979 .
AMA StyleAthanasios Exadactylos, Dimitrios Vafidis, Costas S. Tsigenopoulos, Georgios A. Gkafas. High Connectivity of the White Seabream (Diplodus sargus, L. 1758) in the Aegean Sea, Eastern Mediterranean Basin. Animals. 2019; 9 (11):979.
Chicago/Turabian StyleAthanasios Exadactylos; Dimitrios Vafidis; Costas S. Tsigenopoulos; Georgios A. Gkafas. 2019. "High Connectivity of the White Seabream (Diplodus sargus, L. 1758) in the Aegean Sea, Eastern Mediterranean Basin." Animals 9, no. 11: 979.
Background Comprehensively detailed information on population dynamics for benthic species is crucial since potential admixture of individuals could shift the genetic subdivision and age structure during a full breeding period. The apparent genetic impact of the potential recruitment strategy of Norway lobsterNephrops norvegicusis still under research. For this reason the present study was focused on genetic variation of the species over a given continuous year period in a semi-enclosed gulf of the Aegean Sea. Results Analyses revealed that the relative smaller size class in females and the apparent faster growth of males may represent a key-role differential strategy for the two sexes, whereas females tend to mature slower. Heterozygosity fitness correlations (HFCs) showed substantially significant associations suggesting that inbreeding depression for females and outbreeding depression for males are the proximate fitness mechanisms, respectively. Conclusions Nephrops norvegicusuniformal genetic composition (background of high gene flow), could be attributed to potential population recolonization, due to a hypothesized passive larval movement from deeper waters, which may suggest that some offspring of local residents and potential male non-breeders from other regions admixture randomly.
Georgios A. Gkafas; Marianthi Hatziioannou; Emmanouil E. Malandrakis; Costas Tsigenopoulos; Ioannis T. Karapanagiotidis; Elena Mente; Dimitrios Vafidis; Athanasios Exadactylos. Heterozygosity fitness correlations and generation interval of the Norway lobster in the Aegean Sea, eastern Mediterranean. Journal of Biological Research-Thessaloniki 2019, 26, 1 -9.
AMA StyleGeorgios A. Gkafas, Marianthi Hatziioannou, Emmanouil E. Malandrakis, Costas Tsigenopoulos, Ioannis T. Karapanagiotidis, Elena Mente, Dimitrios Vafidis, Athanasios Exadactylos. Heterozygosity fitness correlations and generation interval of the Norway lobster in the Aegean Sea, eastern Mediterranean. Journal of Biological Research-Thessaloniki. 2019; 26 (1):1-9.
Chicago/Turabian StyleGeorgios A. Gkafas; Marianthi Hatziioannou; Emmanouil E. Malandrakis; Costas Tsigenopoulos; Ioannis T. Karapanagiotidis; Elena Mente; Dimitrios Vafidis; Athanasios Exadactylos. 2019. "Heterozygosity fitness correlations and generation interval of the Norway lobster in the Aegean Sea, eastern Mediterranean." Journal of Biological Research-Thessaloniki 26, no. 1: 1-9.
Acute toxicity and inhibition on growth of Artemia franciscana nauplii (Instar I-II) after exposure to the reference toxicants bisphenol a (BPA) and sodium dodecyl sulfate (SDS) were studied. LC50 values were calculated and differences in body growth were recorded after 24, 48, and 72 h of exposure to the toxicants. The results indicated that BPA had lower toxicity than SDS. Development of the nauplii was clearly influenced by duration of exposure. Growth inhibition was detected for both toxicants. Abnormal growth of the central eye of several Artemia nauplii after 72 h of exposure to BPA was also detected. Our results indicate that growth inhibition could be used as a valid endpoint for toxicity studies.
George Ekonomou; Alexios Lolas; Jeanne Castritsi-Catharios; Christos Neofitou; George Zouganelis; Nikolaos Tsiropoulos; Athanasios Exadactylos. Mortality and Effect on Growth of Artemia franciscana Exposed to Two Common Organic Pollutants. Water 2019, 11, 1614 .
AMA StyleGeorge Ekonomou, Alexios Lolas, Jeanne Castritsi-Catharios, Christos Neofitou, George Zouganelis, Nikolaos Tsiropoulos, Athanasios Exadactylos. Mortality and Effect on Growth of Artemia franciscana Exposed to Two Common Organic Pollutants. Water. 2019; 11 (8):1614.
Chicago/Turabian StyleGeorge Ekonomou; Alexios Lolas; Jeanne Castritsi-Catharios; Christos Neofitou; George Zouganelis; Nikolaos Tsiropoulos; Athanasios Exadactylos. 2019. "Mortality and Effect on Growth of Artemia franciscana Exposed to Two Common Organic Pollutants." Water 11, no. 8: 1614.
Over 80% of the aquaculture production in Europe originates from selective breeding programs aiming to improve several traits simultaneously. Selecting for increased feed efficiency combines economic benefits and a reduced environmental impact. Additionally, disease outbreaks pose serious challenges for organic aquaculture with limited therapeutic agents. Therefore, selective breeding comprises a valuable tool combined with strict biosecurity and effective management practices. Phenotypic sex is labile in fish exhibiting sexual dimorphism in a range of traits of interest. Identified genomic regions concerning sex determination demonstrates the importance of selective breeding. The existence of GxE interactions imply that fish derived from the single nucleus might show lower-than-expected genetic gains in different environments. Understanding their key contribution among different environments is critical for the optimization of a selective breeding program. Next-generation sequencing (NGS) techniques have resulted in the annotation of many teleost genomes, and omics are becoming a powerful multidisciplinary strategy (genomics, transcriptomics, proteomics, and metabolomics). Given the importance of selective breeding programs, particular attention should be paid to the continuous enhancement and development of scientific knowledge, so that the EU legislative framework on organic aquaculture is always up to new challenges toward sustainability increasing the organic logo credibility for the consumers.
Petros V. Martsikalis; Georgios A. Gkafas; Christos Palaiokostas; Athanasios Exadactylos. Genomics Era on Breeding Aquaculture Stocks. Organic Aquaculture 2019, 65 -77.
AMA StylePetros V. Martsikalis, Georgios A. Gkafas, Christos Palaiokostas, Athanasios Exadactylos. Genomics Era on Breeding Aquaculture Stocks. Organic Aquaculture. 2019; ():65-77.
Chicago/Turabian StylePetros V. Martsikalis; Georgios A. Gkafas; Christos Palaiokostas; Athanasios Exadactylos. 2019. "Genomics Era on Breeding Aquaculture Stocks." Organic Aquaculture , no. : 65-77.
Common sole (Solea solea) aquaculture production is based mostly on wild-caught breeders. Recently, the successful reproduction of first-generation fish that were reared in captivity was accomplished. A consistent good quality and quantity of produced eggs throughout the year, and of next-generation broodstock, is important for reducing the overall cost of production. Hox genes play a pivotal role in normal embryonic development and alterations of their temporal expression level may be important for egg viability. Expression profile analysis of five hox genes (hoxa1a, hoxa2a, hoxa2b, hoxb1a and hoxb1b) involved in early embryonic development and of hoxa13a, which is involved in late stages, was carried out. Results revealed a premature and/or maternal expression of hoxa13a in sole embryos, and the detection of hoxa2a and hoxa2b genes as members of paralog group 2. Principal Component Analysis of hox gene expression in 54 ± 6 hours post fertilization embryos coming from wild-caught broodstock and a first-generation one reared in the hatchery, unveiled that these broodstocks are clearly distinct. In addition, their pairwise comparison revealed significant differences in the expression levels of hoxb1a and hoxb1b genes. Hox gene regulation during embryonic development could give valuable insight into rearing sole broodstocks with different origin in concert, and also into gaining a steady mass production of eggs, either in quality or quantity, all year round.
Menelaos Kavouras; Emmanouil E. Malandrakis; Eleni Golomazou; Ioannis Konstantinidis; Ewout Blom; Arjan P. Palstra; Konstantinos Anastassiadis; Panagiota Panagiotaki; Athanasios Exadactylos. Hox gene expression profiles during embryonic development of common sole. Animal Biology 2019, 69, 183 -198.
AMA StyleMenelaos Kavouras, Emmanouil E. Malandrakis, Eleni Golomazou, Ioannis Konstantinidis, Ewout Blom, Arjan P. Palstra, Konstantinos Anastassiadis, Panagiota Panagiotaki, Athanasios Exadactylos. Hox gene expression profiles during embryonic development of common sole. Animal Biology. 2019; 69 (2):183-198.
Chicago/Turabian StyleMenelaos Kavouras; Emmanouil E. Malandrakis; Eleni Golomazou; Ioannis Konstantinidis; Ewout Blom; Arjan P. Palstra; Konstantinos Anastassiadis; Panagiota Panagiotaki; Athanasios Exadactylos. 2019. "Hox gene expression profiles during embryonic development of common sole." Animal Biology 69, no. 2: 183-198.
The horse mussel Modiolus barbatus is a marine benthic bivalve, distributed mainly in the Mediterranean basin, that constitutes a fishery product of high economic importance and a promising candidate for aquaculture. The current study provides the first insights regarding the genetic profile of M. barbatus populations from the eastern Mediterranean, by analysis of a partial segment of the mitochondrial COI gene in individuals collected from five sampling localities within the Aegean Sea. To the best of our knowledge, the derived haplotypes represent the first DNA barcodes of M. barbatus from the entire Mediterranean region. Maximum likelihood phylogenetic analysis revealed that M. barbatus from the North Sea and M. barbatus from the eastern Mediterranean may not belong to the same species and as a consequence, there might be three species of the genus Modiolus in Europe. On the other hand, eastern Mediterranean M. barbatus haplotypes were found to be more closely related to the Asian-Pacific Modiolus species. All geographic populations analysed displayed high levels of genetic diversity, in terms of haplotype and nucleotide diversity and a considerable number of unique alleles. Divergence among populations was found at generally low levels, corresponding with the majority of pairwise Fst values not being significant. These findings suggest no population structure and high levels of gene flow, a common feature observed in marine bivalves with long pelagic larval phases.
Ioannis Giantsis; Athanasios Exadactylos; Konstantinos Feidantsis; Basile Michaelidis. First insights towards the population genetic structure and the phylogeographic status of the horse mussel (Modiolus barbatus) from the eastern Mediterranean. Journal of the Marine Biological Association of the United Kingdom 2018, 99, 1111 -1118.
AMA StyleIoannis Giantsis, Athanasios Exadactylos, Konstantinos Feidantsis, Basile Michaelidis. First insights towards the population genetic structure and the phylogeographic status of the horse mussel (Modiolus barbatus) from the eastern Mediterranean. Journal of the Marine Biological Association of the United Kingdom. 2018; 99 (5):1111-1118.
Chicago/Turabian StyleIoannis Giantsis; Athanasios Exadactylos; Konstantinos Feidantsis; Basile Michaelidis. 2018. "First insights towards the population genetic structure and the phylogeographic status of the horse mussel (Modiolus barbatus) from the eastern Mediterranean." Journal of the Marine Biological Association of the United Kingdom 99, no. 5: 1111-1118.
The effects of salinity and temperature on the photosynthetic and growth performance of the seagrasses Cymodocea nodosa and Ruppia cirrhosa were studied to understand their local seasonality and distribution. Cymodocea nodosa shoots were collected from Cape Vrasidas, and R. cirrhosa shoots from the coastal lagoon Fanari, all from the Eastern Macedonian and Thrace Region, Greece. Effective quantum yield (ΔF/Fm′), leaf chlorophyll-a content (mg g−1 wet mass) and growth (% of maximum) were tested at different temperatures (10–40°C) and salinities (5–60). The results showed that: (a) R. cirrhosa was more euryhaline (5–55/60) than C. nodosa (10–50), (b) the upper thermal tolerance of C. nodosa (34–35°C) was higher than that of R. cirrhosa (32–34°C), (c) C. nodosa could not tolerate 10°C, whereas R. cirrhosa could, and (d) the growth optimum of C. nodosa was 30°C and that of R. cirrhosa 20–30°C. The thermal optima and tolerances of growth and photosynthesis confirm the seasonal patterns of R. cirrhosa but not of C. nodosa. However, the sensitivity of C. nodosa to low salinities and temperatures may explain its absence from shallow coastal lagoons. Ruppia cirrhosa could be vulnerable to future climate change.
Soultana Tsioli; Sotiris Orfanidis; Vasillis Papathanasiou; Christos Katsaros; Athanasios Exadactylos. Effects of salinity and temperature on the performance of Cymodocea nodosa and Ruppia cirrhosa: a medium-term laboratory study. Botanica Marina 2018, 62, 97 -108.
AMA StyleSoultana Tsioli, Sotiris Orfanidis, Vasillis Papathanasiou, Christos Katsaros, Athanasios Exadactylos. Effects of salinity and temperature on the performance of Cymodocea nodosa and Ruppia cirrhosa: a medium-term laboratory study. Botanica Marina. 2018; 62 (2):97-108.
Chicago/Turabian StyleSoultana Tsioli; Sotiris Orfanidis; Vasillis Papathanasiou; Christos Katsaros; Athanasios Exadactylos. 2018. "Effects of salinity and temperature on the performance of Cymodocea nodosa and Ruppia cirrhosa: a medium-term laboratory study." Botanica Marina 62, no. 2: 97-108.