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Invasion and metastasis correspond to the foremost cause of cancer-related death, and the molecular networks behind these two processes are extremely complex and dependent on the intra- and extracellular conditions along with the prime of the premetastatic niche. Currently, several studies suggest an association between the levels of HOX genes expression and cancer cell invasion and metastasis, which favour the formation of novel tumour masses. The deregulation of HOX genes by HMGA2/TET1 signalling and the regulatory effect of noncoding RNAs generated by the HOX loci can also promote invasion and metastasis, interfering with the expression of HOX genes or other genes relevant to these processes. In this review, we present five molecular mechanisms of HOX deregulation by which the HOX clusters products may affect invasion and metastatic processes in solid tumours.
Ana Paço; Simone Aparecida De Bessa Garcia; Joana Leitão Castro; Ana Costa-Pinto; Renata Freitas. Roles of the HOX Proteins in Cancer Invasion and Metastasis. Cancers 2020, 13, 10 .
AMA StyleAna Paço, Simone Aparecida De Bessa Garcia, Joana Leitão Castro, Ana Costa-Pinto, Renata Freitas. Roles of the HOX Proteins in Cancer Invasion and Metastasis. Cancers. 2020; 13 (1):10.
Chicago/Turabian StyleAna Paço; Simone Aparecida De Bessa Garcia; Joana Leitão Castro; Ana Costa-Pinto; Renata Freitas. 2020. "Roles of the HOX Proteins in Cancer Invasion and Metastasis." Cancers 13, no. 1: 10.
Manganese (Mn) toxicity is a very common soil stress around the world, which is responsible for low soil fertility. This manuscript evaluates the effect of the endophytic bacterium Pseudomonas sp. Q1 on different rhizobial-legume symbioses in the absence and presence of Mn toxicity. Three legume species, Cicer arietinum (chickpea), Trifolium subterraneum (subterranean clover), and Medicago polymorpha (burr medic) were used. To evaluate the role of 1-aminocyclopropane-1-carboxylate (ACC) deaminase produced by strain Q1 in these interactions, an ACC deaminase knockout mutant of this strain was constructed and used in those trials. The Q1 strain only promoted the symbiotic performance of Rhizobium leguminosarum bv. trifolii ATCC 14480T and Ensifer meliloti ATCC 9930T, leading to an increase of the growth of their hosts in both conditions. Notably, the acdS gene disruption of strain Q1 abolished the beneficial effect of this bacterium as well as causing this mutant strain to act deleteriously in those specific symbioses. This study suggests that the addition of non-rhizobia with functional ACC deaminase may be a strategy to improve the pasture legume–rhizobial symbioses, particularly when the use of rhizobial strains alone does not yield the expected results due to their difficulty in competing with native strains or in adapting to inhibitory soil conditions.
Ana Paço; José Rodrigo Da-Silva; Denise Pereira Torres; Bernard R. Glick; Clarisse Brígido. Exogenous ACC Deaminase Is Key to Improving the Performance of Pasture Legume-Rhizobial Symbioses in the Presence of a High Manganese Concentration. Plants 2020, 9, 1630 .
AMA StyleAna Paço, José Rodrigo Da-Silva, Denise Pereira Torres, Bernard R. Glick, Clarisse Brígido. Exogenous ACC Deaminase Is Key to Improving the Performance of Pasture Legume-Rhizobial Symbioses in the Presence of a High Manganese Concentration. Plants. 2020; 9 (12):1630.
Chicago/Turabian StyleAna Paço; José Rodrigo Da-Silva; Denise Pereira Torres; Bernard R. Glick; Clarisse Brígido. 2020. "Exogenous ACC Deaminase Is Key to Improving the Performance of Pasture Legume-Rhizobial Symbioses in the Presence of a High Manganese Concentration." Plants 9, no. 12: 1630.
HOX genes are commonly known for their role in embryonic development, defining the positional identity of most structures along the anterior–posterior axis. In postembryonic life, HOX gene aberrant expression can affect several processes involved in tumorigenesis such as proliferation, apoptosis, migration and invasion. Epigenetic modifications are implicated in gene expression deregulation, and it is accepted that methylation events affecting HOX gene expression play crucial roles in tumorigenesis. In fact, specific methylation profiles in the HOX gene sequence or in HOX-associated histones are recognized as potential biomarkers in several cancers, helping in the prediction of disease outcomes and adding information for decisions regarding the patient’s treatment. The methylation of some HOX genes can be associated with chemotherapy resistance, and its identification may suggest the use of other treatment options. The use of epigenetic drugs affecting generalized or specific DNA methylation profiles, an approach that now deserves much attention, seems likely to be a promising weapon in cancer therapy in the near future. In this review, we summarize these topics, focusing particularly on how the regulation of epigenetic processes may be used in cancer therapy.
Ana Paço; Simone Aparecida De Bessa Garcia; Renata Freitas. Methylation in HOX Clusters and Its Applications in Cancer Therapy. Cells 2020, 9, 1613 .
AMA StyleAna Paço, Simone Aparecida De Bessa Garcia, Renata Freitas. Methylation in HOX Clusters and Its Applications in Cancer Therapy. Cells. 2020; 9 (7):1613.
Chicago/Turabian StyleAna Paço; Simone Aparecida De Bessa Garcia; Renata Freitas. 2020. "Methylation in HOX Clusters and Its Applications in Cancer Therapy." Cells 9, no. 7: 1613.
The overgrowth of human population and the demand for high-quality foods necessitate the search for sustainable alternatives to increase crop production. The use of biofertilizers, mostly based on plant probiotic bacteria (PPB), represents a reliable and eco-friendly solution. This heterogeneous group of bacteria possesses many features with positive effects on plants; however, how these bacteria with each other and with the environment when released into a field has still barely been studied. In this review, we focused on the diversity of root endophytic rhizobial and non-rhizobial bacteria existing within plant root tissues, and also on their potential applications as consortia exerting benefits for plants and the environment. We demonstrated the benefits of using bacterial inoculant consortia instead of single-strain inoculants. We then critically discussed several considerations that farmers, companies, governments, and the scientific community should take into account when a biofertilizer based on those PPBs is proposed, including (i) a proper taxonomic identification, (ii) the characterization of the beneficial features of PPB strains, and (iii) the ecological impacts on plants, environment, and plant/soil microbiomes. Overall, the success of a PPB consortium depends on many factors that must be considered and analyzed before its application as a biofertilizer in an agricultural system.
Esther Menéndez; Ana Paço. Is the Application of Plant Probiotic Bacterial Consortia Always Beneficial for Plants? Exploring Synergies between Rhizobial and Non-Rhizobial Bacteria and Their Effects on Agro-Economically Valuable Crops. Life 2020, 10, 24 .
AMA StyleEsther Menéndez, Ana Paço. Is the Application of Plant Probiotic Bacterial Consortia Always Beneficial for Plants? Exploring Synergies between Rhizobial and Non-Rhizobial Bacteria and Their Effects on Agro-Economically Valuable Crops. Life. 2020; 10 (3):24.
Chicago/Turabian StyleEsther Menéndez; Ana Paço. 2020. "Is the Application of Plant Probiotic Bacterial Consortia Always Beneficial for Plants? Exploring Synergies between Rhizobial and Non-Rhizobial Bacteria and Their Effects on Agro-Economically Valuable Crops." Life 10, no. 3: 24.
Eukaryotic genomes are rich in repetitive DNA sequences grouped in two classes regarding their genomic organization: tandem repeats and dispersed repeats. In tandem repeats, copies of a short DNA sequence are positioned one after another within the genome, while in dispersed repeats, these copies are randomly distributed. In this review we provide evidence that both tandem and dispersed repeats can have a similar organization, which leads us to suggest an update to their classification based on the sequence features, concretely regarding the presence or absence of retrotransposons/transposon specific domains. In addition, we analyze several studies that show that a repetitive element can be remodeled into repetitive non-coding or coding sequences, suggesting (1) an evolutionary relationship among DNA sequences, and (2) that the evolution of the genomes involved frequent repetitive sequence reshuffling, a process that we have designated as a “DNA remodeling mechanism”. The alternative classification of the repetitive DNA sequences here proposed will provide a novel theoretical framework that recognizes the importance of DNA remodeling for the evolution and plasticity of eukaryotic genomes.
Ana Paço; Renata Freitas; Ana Vieira-Da-Silva. Conversion of DNA Sequences: From a Transposable Element to a Tandem Repeat or to a Gene. Genes 2019, 10, 1014 .
AMA StyleAna Paço, Renata Freitas, Ana Vieira-Da-Silva. Conversion of DNA Sequences: From a Transposable Element to a Tandem Repeat or to a Gene. Genes. 2019; 10 (12):1014.
Chicago/Turabian StyleAna Paço; Renata Freitas; Ana Vieira-Da-Silva. 2019. "Conversion of DNA Sequences: From a Transposable Element to a Tandem Repeat or to a Gene." Genes 10, no. 12: 1014.
Bacterial endophytes, a subset of a plant’s microbiota, can facilitate plant growth by a number of different mechanisms. The aims of this study were to assess the diversity and functionality of endophytic bacterial strains from internal root tissues of native legume species grown in two distinct sites in South of Portugal and to evaluate their ability to promote plant growth. Here, 122 endophytic bacterial isolates were obtained from 12 different native legume species. Most of these bacteria possess at least one of the plant growth-promoting features tested in vitro, with indole acetic acid production being the most common feature among the isolates followed by the production of siderophores and inorganic phosphate solubilization. The results of in planta experiments revealed that co-inoculation of chickpea plants with specific endophytic bacteria along with N2-fixing symbionts significantly improved the total biomass of chickpea plants, in particular when these plants were grown under saline conditions. Altogether, this study revealed that Mediterranean native legume species are a reservoir of plant growth-promoting bacteria, that are also tolerant to salinity and to toxic levels of Mn. Thus, these bacterial endophytes are well adapted to common constraints present in soils of this region which constitutes important factors to consider in the development of bacterial inoculants for stressful conditions in the Mediterranean region.
Clarisse Brígido; Esther Menéndez; Ana Paço; Bernard R. Glick; Anabela Belo; Maria R. Félix; Solange Oliveira; Mário Carvalho. Mediterranean Native Leguminous Plants: A Reservoir of Endophytic Bacteria with Potential to Enhance Chickpea Growth under Stress Conditions. Microorganisms 2019, 7, 392 .
AMA StyleClarisse Brígido, Esther Menéndez, Ana Paço, Bernard R. Glick, Anabela Belo, Maria R. Félix, Solange Oliveira, Mário Carvalho. Mediterranean Native Leguminous Plants: A Reservoir of Endophytic Bacteria with Potential to Enhance Chickpea Growth under Stress Conditions. Microorganisms. 2019; 7 (10):392.
Chicago/Turabian StyleClarisse Brígido; Esther Menéndez; Ana Paço; Bernard R. Glick; Anabela Belo; Maria R. Félix; Solange Oliveira; Mário Carvalho. 2019. "Mediterranean Native Leguminous Plants: A Reservoir of Endophytic Bacteria with Potential to Enhance Chickpea Growth under Stress Conditions." Microorganisms 7, no. 10: 392.
Evidences for an involvement of the bacterial type IV secretion system (T4SS) in the symbiotic relationship between rhizobia and legumes have been pointed out by several recent studies. However, information regarding this secretion system in Mesorhizobium is still very scarce. The aim of the present study was to investigate the phylogeny and expression of the traG gene, which encodes a substrate receptor of the T4SS. In addition, the occurrence and genomic context of this and other T4SS genes, namely, genes from tra/trb and virB/virD4 complexes, were also analyzed in order to unveil the structural and functional organization of T4SS in mesorhizobia. The location of the T4SS genes in the symbiotic region of the analyzed rhizobial genomes, along with the traG phylogeny, suggests that T4SS genes could be horizontally transferred together with the symbiosis genes. Regarding the T4SS structural organization in Mesorhizobium, the virB/virD4 genes were absent in all chickpea (Cicer arietinum L.) microsymbionts and in the Lotus symbiont Mesorhizobium japonicum MAFF303099T. Interestingly, the presence of genes belonging to another secretion system (T3SS) was restricted to these strains lacking the virB/virD4 genes. The traG gene expression was detected in M. mediterraneum Ca36T and M. ciceri LMS-1 strains when exposed to chickpea root exudates and also in the early nodules formed by M. mediterraneum Ca36T, but not in older nodules. This study contributes to a better understanding of the importance of T4SS in mutualistic symbiotic bacteria.
A. Paço; J. R. Da-Silva; F. Eliziário; C. Brígido; S. Oliveira; A. Alexandre. traG Gene Is Conserved across Mesorhizobium spp. Able to Nodulate the Same Host Plant and Expressed in Response to Root Exudates. BioMed Research International 2019, 2019, 1 -13.
AMA StyleA. Paço, J. R. Da-Silva, F. Eliziário, C. Brígido, S. Oliveira, A. Alexandre. traG Gene Is Conserved across Mesorhizobium spp. Able to Nodulate the Same Host Plant and Expressed in Response to Root Exudates. BioMed Research International. 2019; 2019 ():1-13.
Chicago/Turabian StyleA. Paço; J. R. Da-Silva; F. Eliziário; C. Brígido; S. Oliveira; A. Alexandre. 2019. "traG Gene Is Conserved across Mesorhizobium spp. Able to Nodulate the Same Host Plant and Expressed in Response to Root Exudates." BioMed Research International 2019, no. : 1-13.
Genes in the 5′ extremity of the HoxD cluster encode DNA-binding transcription factors essential for development of the autopod and digits, regulating primarily gene expression and, consequently, morphogenesis and skeletal differentiation. Comparative studies focused on their expression and regulation have led to the idea that evolution of a bimodal regulation of the HoxD cluster, mainly due to the activation of cis-regulatory units in the centromeric side of the cluster, was a fundamental mechanism that potentiated the fin-to-limb transition in vertebrates. In addition, functional assays demonstrated that increased levels of 5′HoxD genes stimulate the production of additional endochondral bone, while repressing the formation of dermal skeleton distally. Other data have come to light in recent years suggesting that these genes may interfere directly with the production of dermal skeleton components in fish and with the activity of cis-regulatory units involved in the formation of autopod and digits. Finally, increasing evidences suggest that the role of HoxD genes in fin evolution may relate to their ability to change the fate of distal mesenchymal cells conducting them to differentiate into endochondral bone rather than in dermal skeleton. Here, we trace the history of the research concerning the involvement of HoxD genes in the fin-to-limb transition in vertebrates. To this end, we discuss three interconnected topics that have benefited from profound advances in recent years due to comparative analyses and functional assays performed using fish species: (a) comparative HoxD genes expression; (b) comparative HoxD gene transcriptional regulation; and (c) functional characterization of 5′HoxD genes.
Ana Paço; Renata Freitas. Hox D genes and the fin-to-limb transition: Insights from fish studies. genesis 2017, 56, e23069 .
AMA StyleAna Paço, Renata Freitas. Hox D genes and the fin-to-limb transition: Insights from fish studies. genesis. 2017; 56 (1):e23069.
Chicago/Turabian StyleAna Paço; Renata Freitas. 2017. "Hox D genes and the fin-to-limb transition: Insights from fish studies." genesis 56, no. 1: e23069.
The ClpB chaperone is known to be involved in bacterial stress response. Moreover, recent studies suggest that this protein has also a role in the chickpea-rhizobia symbiosis. In order to improve both stress tolerance and symbiotic performance of a chickpea microsymbiont, the Mesorhizobium mediterraneum UPM-Ca36T strain was genetically transformed with pPHU231 containing an extra-copy of the clpB gene. To investigate if the clpB-transformed strain displays an improved stress tolerance, bacterial growth was evaluated under heat and acid stress conditions. In addition, the effect of the extra-copies of the clpB gene in the symbiotic performance was evaluated using plant growth assays (hydroponic and pot trials). The clpB-transformed strain is more tolerant to heat shock than the strain transformed with pPHU231, supporting the involvement of ClpB in rhizobia heat shock tolerance. Both plant growth assays showed that ClpB has an important role in chickpea-rhizobia symbiosis. The nodulation kinetics analysis showed a higher rate of nodule appearance with the clpB-transformed strain. This strain also induced a greater number of nodules and, more notably, its symbiotic effectiveness increased ~60% at pH5 and 83% at pH7, compared to the wild-type strain. Furthermore, a higher frequency of root hair curling was also observed in plants inoculated with the clpB-transformed strain, compared to the wild-type strain. The superior root hair curling induction, nodulation ability and symbiotic effectiveness of the clpB-transformed strain may be explained by an increased expression of symbiosis genes. Indeed, higher transcript levels of the nodulation genes nodA and nodC (~3 folds) were detected in the clpB-transformed strain. The improvement of rhizobia by addition of extra-copies of the clpB gene may be a promising strategy to obtain strains with enhanced stress tolerance and symbiotic effectiveness, thus contributing to their success as crop inoculants, particularly under environmental stresses. This is the first report on the successful improvement of a rhizobium with a chaperone gene.
Ana Paço; Clarisse Brígido; Ana Alexandre; Pedro Mateos; Solange Oliveira. The Symbiotic Performance of Chickpea Rhizobia Can Be Improved by Additional Copies of the clpB Chaperone Gene. PLOS ONE 2016, 11, e0148221 .
AMA StyleAna Paço, Clarisse Brígido, Ana Alexandre, Pedro Mateos, Solange Oliveira. The Symbiotic Performance of Chickpea Rhizobia Can Be Improved by Additional Copies of the clpB Chaperone Gene. PLOS ONE. 2016; 11 (2):e0148221.
Chicago/Turabian StyleAna Paço; Clarisse Brígido; Ana Alexandre; Pedro Mateos; Solange Oliveira. 2016. "The Symbiotic Performance of Chickpea Rhizobia Can Be Improved by Additional Copies of the clpB Chaperone Gene." PLOS ONE 11, no. 2: e0148221.
Three novel repetitive DNA sequences are described, presenting a similar heterochromatic chromosomal location in two hamster species: Phodopus roborovskii and Phodopus sungorus (Cricetidae, Rodentia). Namely, two species-specific repetitive sequences (PROsat from P. roborovskii and PSUchr1sat from P. sungorus) surrounding a third one (PsatDNA), that is shared by both hamster genomes. Fiber-FISH analyses revealed that PROsat intermingles with PsatDNA in P. roborovskii and PSUchr1sat intermingles with PsatDNA in P. sungorus. A model explaining the evolution of this intricate chromosomal distribution is proposed, which can explain better the evolution of these very derivative genomes (in comparison to the ancestral Muroidea). The most plausible evolutionary scenario seems to be the expansion of a number of repeats into other’s domain, most probably resulting in its intermingling, followed by the subsequent spread of these complex repeats from a single chromosomal location to other chromosomes. Evidences of an association between repetitive sequences and the chromosome evolution process were observed, namely for PROsat. Most probably, the evolutionary breakpoints that shaped PRO and PSU chromosomes (pericentric inversions and fusions) occurred within the boundaries of PROsat blocks in the ancestor. The repeats high diversity at the heterochromatic regions of Phodopus chromosomes, together with its complex organization, suggests that these species are important models for evolutionary studies, namely in the investigation of a possible relationship between repetitive sequences and the occurrence of chromosomal rearrangements and consequently, in genome evolution.
Ana Paço; Filomena Adega; Nevenka Meštrović; Miroslav Plohl; Raquel Chaves. The puzzling character of repetitive DNA in Phodopus genomes (Cricetidae, Rodentia). Chromosome Research 2015, 23, 427 -440.
AMA StyleAna Paço, Filomena Adega, Nevenka Meštrović, Miroslav Plohl, Raquel Chaves. The puzzling character of repetitive DNA in Phodopus genomes (Cricetidae, Rodentia). Chromosome Research. 2015; 23 (3):427-440.
Chicago/Turabian StyleAna Paço; Filomena Adega; Nevenka Meštrović; Miroslav Plohl; Raquel Chaves. 2015. "The puzzling character of repetitive DNA in Phodopus genomes (Cricetidae, Rodentia)." Chromosome Research 23, no. 3: 427-440.
With the goal to contribute for the understanding of satellite DNA evolution and its genomic involvement, in this work it was isolated and characterized the first satellite DNA (PSUcentSat) from Phodopus sungorus (Cricetidae). Physical mapping of this sequence in P. sungorus showed large PSUcentSat arrays located at the heterochromatic (peri)centromeric region of five autosomal pairs and Y-chromosome. The presence of orthologous PSUcentSat sequences in the genomes of other Cricetidae and Muridae rodents was also verified, presenting however, an interspersed chromosomal distribution. This distribution pattern suggests a PSUcentSat-scattered location in an ancestor of Muridae/Cricetidae families, that assumed afterwards, in the descendant genome of P. sungorus a restricted localization to few chromosomes in the (peri)centromeric region. We believe that after the divergence of the studied species, PSUcentSat was most probably highly amplified in the (peri)centromeric region of some chromosome pairs of this hamster by recombinational mechanisms. The bouquet chromosome configuration (prophase I) possibly displays an important role in this selective amplification, providing physical proximity of centromeric regions between chromosomes with similar size and/or morphology. This seems particularly evident for the acrocentric chromosomes of P. sungorus (including the Y-chromosome), all presenting large PSUcentSat arrays at the (peri)centromeric region. The conservation of this sequence in the studied genomes and its (peri)centromeric amplification in P. sungorus strongly suggests functional significance, possibly displaying this satellite family different functions in the different genomes. The verification of PSUcentSat transcriptional activity in normal proliferative cells suggests that its transcription is not stage-limited, as described for some other satellites.
Ana Paço; Filomena Adega; N. Me Trovi; Miroslav Plohl; Raquel Chaves; Nevenka Meštrović. Evolutionary Story of a Satellite DNA from Phodopus sungorus (Rodentia, Cricetidae). Genome Biology and Evolution 2014, 6, 2944 -2955.
AMA StyleAna Paço, Filomena Adega, N. Me Trovi, Miroslav Plohl, Raquel Chaves, Nevenka Meštrović. Evolutionary Story of a Satellite DNA from Phodopus sungorus (Rodentia, Cricetidae). Genome Biology and Evolution. 2014; 6 (10):2944-2955.
Chicago/Turabian StyleAna Paço; Filomena Adega; N. Me Trovi; Miroslav Plohl; Raquel Chaves; Nevenka Meštrović. 2014. "Evolutionary Story of a Satellite DNA from Phodopus sungorus (Rodentia, Cricetidae)." Genome Biology and Evolution 6, no. 10: 2944-2955.
Long interspersed nuclear elements-1 (LINE-1) are the most abundant and active retrotransposons in the mammalian genomes. Traditionally, the occurrence of LINE-1 sequences in the genome of mammals has been explained by the selfish DNA hypothesis. Nevertheless, recently, it has also been argued that these sequences could play important roles in these genomes, as in the regulation of gene expression, genome modelling and X-chromosome inactivation. The non-random chromosomal distribution is a striking feature of these retroelements that somehow reflects its functionality. In the present study, we have isolated and analysed a fraction of the open reading frame 2 (ORF2) LINE-1 sequence from three rodent species, Cricetus cricetus, Peromyscus eremicus and Praomys tullbergi. Physical mapping of the isolated sequences revealed an interspersed longitudinal AT pattern of distribution along all the chromosomes of the complement in the three genomes. A detailed analysis shows that these sequences are preferentially located in the euchromatic regions, although some signals could be detected in the heterochromatin. In addition, a coincidence between the location of imprinted gene regions (as Xist and Tsix gene regions) and the LINE-1 retroelements was also observed. According to these results, we propose an involvement of LINE-1 sequences in different genomic events as gene imprinting, X-chromosome inactivation and evolution of repetitive sequences located at the heterochromatic regions (e.g. satellite DNA sequences) of the rodents’ genomes analysed.
Ana Paço; Filomena Adega; Raquel Chaves. LINE-1 retrotransposons: from ‘parasite’ sequences to functional elements. Journal of Applied Genetics 2014, 56, 133 -145.
AMA StyleAna Paço, Filomena Adega, Raquel Chaves. LINE-1 retrotransposons: from ‘parasite’ sequences to functional elements. Journal of Applied Genetics. 2014; 56 (1):133-145.
Chicago/Turabian StyleAna Paço; Filomena Adega; Raquel Chaves. 2014. "LINE-1 retrotransposons: from ‘parasite’ sequences to functional elements." Journal of Applied Genetics 56, no. 1: 133-145.
In this work we characterize the Phodopus roborovskii and Phodopus sungorus karyotypes, describing the constitutive heterochromatin and the telomeric repeats distribution in these chromosomes. In the two species, (peri)centromeric, interstitial and (sub)telomeric C-bands were revealed, presenting a very high colocalization with evolutionary breakpoint regions identified in these karyotypes. Also both species present telomeric sequences located interstitially (ITS), as short ITS blocks or as large ITS blocks, mainly at the (peri)centromeric heterochromatic regions. The number and degree of ITSs amplification varies greatly in the two hamsters, indicating independent evolutionary events of these repeats in each genome. The combination of the data provided interesting insights about the genome organization of these hamster species, also allowing establishing evolutionary considerations on their chromosomes. The obtained results clearly suggest an involvement of the repetitive genomic fraction in the reshaping of P. roborovskii and P. sungorus karyotypes.
Ana Paço; Raquel Chaves; Ana Silva; Filomena Adega. The involvement of repetitive sequences in the remodelling of karyotypes: The Phodopus genomes (Rodentia, Cricetidae). Micron 2013, 46, 27 -34.
AMA StyleAna Paço, Raquel Chaves, Ana Silva, Filomena Adega. The involvement of repetitive sequences in the remodelling of karyotypes: The Phodopus genomes (Rodentia, Cricetidae). Micron. 2013; 46 ():27-34.
Chicago/Turabian StyleAna Paço; Raquel Chaves; Ana Silva; Filomena Adega. 2013. "The involvement of repetitive sequences in the remodelling of karyotypes: The Phodopus genomes (Rodentia, Cricetidae)." Micron 46, no. : 27-34.
The use of in situ restriction endonuclease (RE) (which cleaves DNA at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (CH), and in the understanding of the CH evolution in different genomes. In the present work we describe in detail the CH of the three Rodentia species, Cricetus cricetus, Peromyscus eremicus (family Cricetidae) and Praomys tullbergi (family Muridae) using a panel of seven REs followed by C-banding. Comparison of the amount, distribution and molecular nature of C-positive heterochromatin revealed molecular heterogeneity in the heterochromatin of the three species. The large number of subclasses of CH identified in Praomys tullbergi chromosomes indicated that the karyotype of this species is the more derived when compared with the other two genomes analyzed, probably originated by a great number of complex chromosomal rearrangements. The high level of sequence heterogeneity identified in the CH of the three genomes suggests the coexistence of different satellite DNA families, or variants of these families in these genomes
Ana Paço; Filomena Adega; Henrique Guedes-Pinto; Raquel Chaves. Hidden heterochromatin: characterization in the Rodentia species Cricetus cricetus, Peromyscus eremicus (Cricetidae) and Praomys tullbergi (Muridae). Genetics and Molecular Biology 2009, 32, 56 -68.
AMA StyleAna Paço, Filomena Adega, Henrique Guedes-Pinto, Raquel Chaves. Hidden heterochromatin: characterization in the Rodentia species Cricetus cricetus, Peromyscus eremicus (Cricetidae) and Praomys tullbergi (Muridae). Genetics and Molecular Biology. 2009; 32 (1):56-68.
Chicago/Turabian StyleAna Paço; Filomena Adega; Henrique Guedes-Pinto; Raquel Chaves. 2009. "Hidden heterochromatin: characterization in the Rodentia species Cricetus cricetus, Peromyscus eremicus (Cricetidae) and Praomys tullbergi (Muridae)." Genetics and Molecular Biology 32, no. 1: 56-68.
Constitutive heterochromatin comprises a substantial fraction of the eukaryotic genomes and is mainly composed of tandemly arrayed satellite DNAs (satDNA). These repetitive sequences represent a very dynamic and fast evolving component of genomes. In the present work we report the isolation of Cricetus cricetus (CCR, Cricetidae, Rodentia) centromeric repetitive sequences from chromosome 4 (CCR4/10sat), using the laser microdissection and laser pressure catapulting procedure, followed by DOP-PCR amplification and labelling. Physical mapping by fluorescent in situ hybridisation of these sequences onto C. cricetus and another member of Cricetidae, Peromyscus eremicus, displayed quite interesting patterns. Namely, the centromeric sequences showed to be present in another C. cricetus chromosome (CCR10) besides CCR4. Moreover, these almost chromosome-specific sequences revealed to be present in the P. eremicus genome, and most interestingly, displaying a ubiquitous scattered distribution throughout this karyotype. Finally and in both species, a co-localisation of CCR4/10sat with constitutive heterochromatin was found, either by classical C-banding or C-banding sequential to in situ endonuclease restriction. The presence of these orthologous sequences in both genomes is suggestive of a phylogenetic proximity. Furthermore, the existence of common repetitive DNA sequences with a different chromosomal location foresees the occurrence of an extensive process of karyotype restructuring somehow related with intragenomic movements of these repetitive sequences during the evolutionary process of C. cricetus and P. eremicus species.
Sandra Louzada; Ana Paço; Svatava Kubickova; Filomena Adega; Henrique Guedes-Pinto; Jiri Rubes; Raquel Chaves. Different evolutionary trails in the related genomes Cricetus cricetus and Peromyscus eremicus (Rodentia, Cricetidae) uncovered by orthologous satellite DNA repositioning. Micron 2008, 39, 1149 -1155.
AMA StyleSandra Louzada, Ana Paço, Svatava Kubickova, Filomena Adega, Henrique Guedes-Pinto, Jiri Rubes, Raquel Chaves. Different evolutionary trails in the related genomes Cricetus cricetus and Peromyscus eremicus (Rodentia, Cricetidae) uncovered by orthologous satellite DNA repositioning. Micron. 2008; 39 (8):1149-1155.
Chicago/Turabian StyleSandra Louzada; Ana Paço; Svatava Kubickova; Filomena Adega; Henrique Guedes-Pinto; Jiri Rubes; Raquel Chaves. 2008. "Different evolutionary trails in the related genomes Cricetus cricetus and Peromyscus eremicus (Rodentia, Cricetidae) uncovered by orthologous satellite DNA repositioning." Micron 39, no. 8: 1149-1155.