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Eren Ozcagli
Istanbul University , Faculty of Pharmacy , Department of Pharmaceutical Toxicology , 34116 , Beyazıt , Istanbul , Turkey .; ; Tel: +902124400000

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Article
Published: 12 August 2019 in Toxicology Research
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Celastrol is a natural bioactive compound extracted from the medicinal plant Tripterygium wilfordii Hook F. It exhibits immunosuppressive, anti-inflammatory, and antioxidant activities. Cisplatin is a commonly used chemotherapeutic drug in the treatment of a wide range of tumors. Although very effective therapeutically, it can cause nephrotoxicity leading to dose reduction or discontinuation of treatment. This study aims to clarify the therapeutic potential of celastrol in cisplatin-induced nephrotoxicity. The possible protective effects of celastrol pretreatment against cisplatin-induced oxidative stress and genotoxicity were investigated. A rat kidney epithelial cell line NRK-52E was pretreated with the desired concentrations of celastrol (200 nM, 100 nM, and 50 nM) for 24 h. The cells were treated with 50 μM cisplatin for a further 24 h to see whether cisplatin caused the same or less toxicity compared to the vehicle control group. Alkaline comet assay was performed for genotoxicity assessment. Genotoxicity evaluation revealed that celastrol caused a statistically significant reduction in DNA damage. Oxidative stress parameters were evaluated by measuring the glutathione (GSH) and protein carbonyl (PC) levels and also by measuring the enzyme activities of glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD) enzymes. Celastrol pretreatment increased the GSH content of the cells and ameliorated the protein carbonylation level. Likewise, celastrol pretreatment improved the GR and CAT activities. However, no significant difference was observed in GPx and SOD activities. In the light of these findings, celastrol treatment could be a therapeutic option to reduce cisplatin-induced nephrotoxicity. Further studies are needed for the clarification of its therapeutic potential.

ACS Style

Tugce Boran; Aysenur Gunaydin; Ayse Tarbin Jannuzzi; Eren Ozcagli; Buket Alpertunga. Celastrol pretreatment as a therapeutic option against cisplatin-induced nephrotoxicity. Toxicology Research 2019, 8, 723 -730.

AMA Style

Tugce Boran, Aysenur Gunaydin, Ayse Tarbin Jannuzzi, Eren Ozcagli, Buket Alpertunga. Celastrol pretreatment as a therapeutic option against cisplatin-induced nephrotoxicity. Toxicology Research. 2019; 8 (5):723-730.

Chicago/Turabian Style

Tugce Boran; Aysenur Gunaydin; Ayse Tarbin Jannuzzi; Eren Ozcagli; Buket Alpertunga. 2019. "Celastrol pretreatment as a therapeutic option against cisplatin-induced nephrotoxicity." Toxicology Research 8, no. 5: 723-730.

Article
Published: 01 February 2019 in Pharmacogenetics and Genomics
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CYP2D6 metabolizes ∼25% of all clinically used drugs, with numerous genetic polymorphisms affecting enzyme activity and drug response. Clinical utility of current CYP2D6 genotyping is partially compromised the unresolved complex haplotype structure of the CYP2D6 locus. We have identified a distal enhancer single-nucleotide polymorphism rs5758550 that robustly increases CYP2D6 expression, whereas rs16947 (CYP2D6*2), previously considered inert, reduces correct mRNA splicing and expression, thereby affecting presumed activity of other alleles on the *2 haplotype. This study aims to determine the structure and frequency of haplotypes containing either rs5758550 or rs16947, or both, together with other relevant CYP2D6 alleles, assigning predictive enzyme activity scores to each, and addressing ambiguities in estimating diplotypes in different populations. The structure and frequency of haplotypes containing rs5758550 and/or rs16947 in different populations were determined by using phased genotype data from ‘The 1000 Genomes Project’. The assigned haplotype–phenotype relationship was tested by associating assigned CYP2D6 activity score with CYP2D6 enzyme activity in a cohort of 122 human liver microsomes. Addition of enhancer single-nucleotide polymorphism rs5758550 and *2 to a CYP2D6 panel improves prediction of CYP2D6 activity. Moreover, the haplotype containing rs5758550 and rs16947 predict extensive CYP2D6 activity more accurately than CYP2D6*2A, a surrogate marker for extensive activity. With further studies, the results support possible incorporation of rs5758550 and rs16947 into CYP2D6 biomarker panels for more accurate prediction of CYP2D6 metabolizer status.

ACS Style

Balmiki Ray; Eren Ozcagli; Wolfgang Sadee; Danxin Wang. CYP2D6 haplotypes with enhancer single-nucleotide polymorphism rs5758550 and rs16947 (*2 allele). Pharmacogenetics and Genomics 2019, 29, 39 -47.

AMA Style

Balmiki Ray, Eren Ozcagli, Wolfgang Sadee, Danxin Wang. CYP2D6 haplotypes with enhancer single-nucleotide polymorphism rs5758550 and rs16947 (*2 allele). Pharmacogenetics and Genomics. 2019; 29 (2):39-47.

Chicago/Turabian Style

Balmiki Ray; Eren Ozcagli; Wolfgang Sadee; Danxin Wang. 2019. "CYP2D6 haplotypes with enhancer single-nucleotide polymorphism rs5758550 and rs16947 (*2 allele)." Pharmacogenetics and Genomics 29, no. 2: 39-47.

Journal article
Published: 01 June 2018 in Steroids
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Stanozolol is a widely used 17α-alkylated anabolic androgenic steroid (AAS) derivative. Despite stanozolol's adverse effects, its effect on oxidative stress parameters and mitochondrial apoptosis pathway is not clearly defined. In our study, thirty four male Sprague-Dawley rats were divided into 5 groups as control (C), vehicle control (VC), steroid (ST), vehicle control-exercise (VCE), and steroid-exercise (STE). Animals were subcutaneously administered stanozolol 5 mg/kg in steroid groups and propylene glycol 1 ml/kg in the vehicle-control groups. On the 28 day-after sacrification, oxidative stress (MDA, GSH, PC, SOD, CAT) and apoptosis parameters (TUNEL, Cytochrome-c) in cardiac tissue were evaluated. Also, blood vessel morphology of cardiac tissue was evaluated with Verhoeff-van Giesen staining. It has been demonstrated that stanozolol administration triggers apoptosis by using TUNEL assay and cytochrome-c immunohistochemical staining intensity, while this effect is significantly reduced in the presence of exercise. In conclusion, the present study demonstrated that stanozolol administration induces apoptosis with increasing PC and CAT levels, while GSH, MDA and SOD parameters do not reveal any significant change. Exercise has a protective role in stanozolol induced oxidative stress and apoptosis. According to Verhoeff -van Giesen staining results for blood vessel morphology assessment, it has been seen that exercise has a protective role on cardiac blood vessels. This mechanism needs further investigations with long term exposure studies for clarifying possible pathways.

ACS Style

Mehtap Kara; Eren Ozcagli; Tuğba Kotil; Buket Alpertunga. Effects of stanozolol on apoptosis mechanisms and oxidative stress in rat cardiac tissue. Steroids 2018, 134, 96 -100.

AMA Style

Mehtap Kara, Eren Ozcagli, Tuğba Kotil, Buket Alpertunga. Effects of stanozolol on apoptosis mechanisms and oxidative stress in rat cardiac tissue. Steroids. 2018; 134 ():96-100.

Chicago/Turabian Style

Mehtap Kara; Eren Ozcagli; Tuğba Kotil; Buket Alpertunga. 2018. "Effects of stanozolol on apoptosis mechanisms and oxidative stress in rat cardiac tissue." Steroids 134, no. : 96-100.

Journal article
Published: 01 May 2018 in OMICS: A Journal of Integrative Biology
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Human papillomavirus (HPV) infection is one of the most common sexually transmitted diseases worldwide and a prime cause of cervical cancer. The HPV DNA is detected in approximately 80-90% of all cervical cancers, with HPV 16 and 18 being the high risk conferring human carcinogens. DNA damage and diminished DNA repair mechanisms are potential biological surrogates of HPV infection that warrant further research in different tissues and populations. Notably, we do not know the extent to which the high risk HPV 16 and 18 differentially affect cervical cells versus other systems such as peripheral blood lymphocytes (PBLs). We evaluated DNA damage and repair in women who tested positive for HPV 16 or HPV 18 and healthy control women without HPV 16 or HPV 18 infection. We found that the DNA damage as measured by the Comet assay was markedly greater in cervical cells of women with HPV 16 (mean: 8.1 as% DNA in tail, 95% CI: 7.6-8.7) or HPV 18 infection (mean: 9.6, 95% CI: 8.9-10.2) than controls (mean: 6.7, 95% CI: 6.2-7.4) (p < 0.05). By contrast, in PBLs, we did not find a significant difference in DNA damage between women with HPV 16 or 18 infection versus controls, as measured by the Comet assay or the Conventional Chromosomal Aberration analysis (p > 0.05). We observed, however, the DNA repair capacity, as measured by the X-ray induced challenge (XRC) assay, was significantly impaired in PBLs from women with HPV 16 or 18 infection compared to controls (p < 0.05). This is the first comparative study, to the best of our knowledge, suggesting that the cervical swab cells might be better suited than peripheral lymphocytes as biosamples for detection of HPV 16 or 18 biological effects on DNA damage. In addition, these findings suggest that the Comet assay performed only in PBLs may potentially lead to false negative diagnosis of DNA damage. Taken together, these observations contribute to development of future diagnostic innovation and precision sampling strategies for robust detection of the biological effects of HPV 16 or 18 in women. We conclude by a brief discussion of implications for HPV clinical diagnostics and precision medicine innovation.

ACS Style

Eren Ozcagli; Aydan Biri; Bedia Dinç; Semra Sardas; Eren Özçağlı. How Does Infection with Human Papillomavirus 16 and 18 Impact on DNA Damage and Repair in Cervical Cells and Peripheral Blood? OMICS: A Journal of Integrative Biology 2018, 22, 332 -336.

AMA Style

Eren Ozcagli, Aydan Biri, Bedia Dinç, Semra Sardas, Eren Özçağlı. How Does Infection with Human Papillomavirus 16 and 18 Impact on DNA Damage and Repair in Cervical Cells and Peripheral Blood? OMICS: A Journal of Integrative Biology. 2018; 22 (5):332-336.

Chicago/Turabian Style

Eren Ozcagli; Aydan Biri; Bedia Dinç; Semra Sardas; Eren Özçağlı. 2018. "How Does Infection with Human Papillomavirus 16 and 18 Impact on DNA Damage and Repair in Cervical Cells and Peripheral Blood?" OMICS: A Journal of Integrative Biology 22, no. 5: 332-336.

Journal article
Published: 26 April 2018 in International Journal of Molecular Medicine
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Anabolic agents are doping substances which are commonly used in sports. Stanozolol, a 17α-alkylated derivative of testosterone, has a widespread use among athletes and bodybuilders. Several medical and behavioral adverse effects are associated with anabolic androgenic steroids (AAS) abuse, while the liver remains the most well recognized target organ. In the present study, the hepatic effects of stanozolol administration in rats at high doses resembling those used for doping purposes were investigated, in the presence or absence of exercise. Stanozolol and its metabolites, 16-β-hydroxystanozolol and 3′-hydroxystanozolol, were detected in rat livers using liquid chromatography-mass spectrometry (LC-MS). Telomerase activity, which is involved in cellular aging and tumorigenesis, was detected by examining telomerase reverse transcriptase (TERT) and phosphatase and tensin homolog (PTEN) expression levels in the livers of stanozolol-treated rats. Stanozolol induced telomerase activity at the molecular level in the liver tissue of rats and exercise reversed this induction, reflecting possible premature liver tissue aging. PTEN gene expression in the rat livers was practically unaffected either by exercise or by stanozolol administration.

ACS Style

Eren Ozcagli; Mehtap Kara; Tugba Kotil; Persefoni Fragkiadaki; Manolis N. Tzatzarakis; Christina Tsitsimpikou; Polychronis D. Stivaktakis; Dimitris Tsoukalas; Demetrios Spandidos; Aristidis Tsatsakis; Buket Alpertunga. Stanozolol administration combined with exercise leads to decreased telomerase activity possibly associated with liver aging. International Journal of Molecular Medicine 2018, 42, 405 -413.

AMA Style

Eren Ozcagli, Mehtap Kara, Tugba Kotil, Persefoni Fragkiadaki, Manolis N. Tzatzarakis, Christina Tsitsimpikou, Polychronis D. Stivaktakis, Dimitris Tsoukalas, Demetrios Spandidos, Aristidis Tsatsakis, Buket Alpertunga. Stanozolol administration combined with exercise leads to decreased telomerase activity possibly associated with liver aging. International Journal of Molecular Medicine. 2018; 42 (1):405-413.

Chicago/Turabian Style

Eren Ozcagli; Mehtap Kara; Tugba Kotil; Persefoni Fragkiadaki; Manolis N. Tzatzarakis; Christina Tsitsimpikou; Polychronis D. Stivaktakis; Dimitris Tsoukalas; Demetrios Spandidos; Aristidis Tsatsakis; Buket Alpertunga. 2018. "Stanozolol administration combined with exercise leads to decreased telomerase activity possibly associated with liver aging." International Journal of Molecular Medicine 42, no. 1: 405-413.

Journal article
Published: 19 March 2018 in Mutation Research/Genetic Toxicology and Environmental Mutagenesis
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Imidacloprid (IMI) is a systemic, chloro-nicotinyl insecticide classified in Regulation N° 1272/2008 of the European Commision as “harmful if swallowed and very toxic to aquatic life, with long-lasting effects”. IMI is metabolized in vitro both by aldehyde oxidase (AOX) (reduction) and by cytochrome P450s enzymes (CYPs). In the present study, the AOX inhibitor sodium tungstate dihydrate (ST) was used to elucidate the relative contribution of CYP 450 and AOX metabolic pathways on IMI metabolism, in male rabbits exposed to IMI for two months. To evaluate the inhibition effectiveness, various metabolite concentrations in the IMI and IMI + ST exposed groups were monitored. DNA damage was also evaluated in micronucleus (MN) and single cell electrophoresis (SCGC) assays in both groups, along with oxidative stress (OS) with the inflammatory status of the exposed animals, in order to clarify which metabolic pathway is more detrimental in this experimental setting. A significant increase in the frequency of binucleated cells with MN (BNMN, 105%) and micronuclei (MN, 142%) was observed after exposure to IMI (p < 0.001). The increase in the ST co-exposed animals was less pronounced (BNMN 75%, MN 95%). The Cytokinesis Block Proliferation Index (CBPI) showed no significant difference between controls and exposed animals at any time of exposure (p > 0.05), which indicates no cytotoxic effect. Similarly, comet results show that the IMI group exhibited the highest achieved tail intensity, which reached 70.7% over the control groups, whereas in the IMI + ST groups the increase remained at 48.5%. No differences were observed between all groups for oxidative-stress biomarkers. The results indicate that the AOX metabolic pathway plays a more important role in the systemic toxicity of IMI.

ACS Style

Alexander I. Vardavas; Eren Ozcagli; Persefoni Fragkiadaki; Polychronis D. Stivaktakis; Manolis N. Tzatzarakis; Athanasios K. Alegakis; Fotini Vasilaki; Kostas Kaloudis; John Tsiaoussis; Dimitrios Kouretas; Christina Tsitsimpikou; Félix Carvalho; Aristidis M. Tsatsakis. The metabolism of imidacloprid by aldehyde oxidase contributes to its clastogenic effect in New Zealand rabbits. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 2018, 829-830, 26 -32.

AMA Style

Alexander I. Vardavas, Eren Ozcagli, Persefoni Fragkiadaki, Polychronis D. Stivaktakis, Manolis N. Tzatzarakis, Athanasios K. Alegakis, Fotini Vasilaki, Kostas Kaloudis, John Tsiaoussis, Dimitrios Kouretas, Christina Tsitsimpikou, Félix Carvalho, Aristidis M. Tsatsakis. The metabolism of imidacloprid by aldehyde oxidase contributes to its clastogenic effect in New Zealand rabbits. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2018; 829-830 ():26-32.

Chicago/Turabian Style

Alexander I. Vardavas; Eren Ozcagli; Persefoni Fragkiadaki; Polychronis D. Stivaktakis; Manolis N. Tzatzarakis; Athanasios K. Alegakis; Fotini Vasilaki; Kostas Kaloudis; John Tsiaoussis; Dimitrios Kouretas; Christina Tsitsimpikou; Félix Carvalho; Aristidis M. Tsatsakis. 2018. "The metabolism of imidacloprid by aldehyde oxidase contributes to its clastogenic effect in New Zealand rabbits." Mutation Research/Genetic Toxicology and Environmental Mutagenesis 829-830, no. : 26-32.

Journal article
Published: 01 October 2017 in Toxicology Letters
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ACS Style

Tugce Boran; Aysenur Gunaydin; Ayse Tarbin Jannuzzi; Eren Ozcagli; Buket Alpertunga. Assessing the role of celastrole on cisplatin induced nephrotoxicity under in vitro conditions. Toxicology Letters 2017, 280, S241 .

AMA Style

Tugce Boran, Aysenur Gunaydin, Ayse Tarbin Jannuzzi, Eren Ozcagli, Buket Alpertunga. Assessing the role of celastrole on cisplatin induced nephrotoxicity under in vitro conditions. Toxicology Letters. 2017; 280 ():S241.

Chicago/Turabian Style

Tugce Boran; Aysenur Gunaydin; Ayse Tarbin Jannuzzi; Eren Ozcagli; Buket Alpertunga. 2017. "Assessing the role of celastrole on cisplatin induced nephrotoxicity under in vitro conditions." Toxicology Letters 280, no. : S241.

Review article
Published: 02 June 2017 in DARU Journal of Pharmaceutical Sciences
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The discovery of a "new" psychoactive substance is a relatively exceptional event, while the regulatory response usually involved the assessment of risks to public health and inclusion of the novel substance in the national list of controlled substances. However, in recent years we have witnessed the rapid emergence of new chemical substances, which elude international control and pose a challenge to existing processes and a threat to the credibility of control systems. We currently review and present characteristics of these legal and illegal new substances and issues regarding their global monitoring and regulatory measures already taken, or in the process of being taken, for their control. The concept of prohibition applied in active substance-related legislation is rather hazard ridden as balance is required between the ban on substances of potential therapeutic use and the access on the market of high-risk substances. Current and future laws regarding psychoactive compounds.

ACS Style

Carolina Negrei; Bianca Galateanu; Miriana Stan; Cristian Balalau; Mircea Lucian Bogdan Dumitru; Eren Ozcagli; Concettina Fenga; Leda Kovatsi; Domniki Fragou; Aristidis Tsatsakis. Worldwide legislative challenges related to psychoactive drugs. DARU Journal of Pharmaceutical Sciences 2017, 25, 14 .

AMA Style

Carolina Negrei, Bianca Galateanu, Miriana Stan, Cristian Balalau, Mircea Lucian Bogdan Dumitru, Eren Ozcagli, Concettina Fenga, Leda Kovatsi, Domniki Fragou, Aristidis Tsatsakis. Worldwide legislative challenges related to psychoactive drugs. DARU Journal of Pharmaceutical Sciences. 2017; 25 (1):14.

Chicago/Turabian Style

Carolina Negrei; Bianca Galateanu; Miriana Stan; Cristian Balalau; Mircea Lucian Bogdan Dumitru; Eren Ozcagli; Concettina Fenga; Leda Kovatsi; Domniki Fragou; Aristidis Tsatsakis. 2017. "Worldwide legislative challenges related to psychoactive drugs." DARU Journal of Pharmaceutical Sciences 25, no. 1: 14.

Review article
Published: 17 April 2017 in DARU Journal of Pharmaceutical Sciences
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Physicians often come across with cases of vitamin K antagonists–dependent coagulopathy for reasons such as accidental use of the vitamin K antagonists (VKA), excessive administration of prescribed anticoagulants of indirect action or not reported administration of vitamin K antagonists due to memory impairment and/or other mental disorders, even deliberate use thereof (attempt to murder or suicide). Rodenticide-poisoning (coumarins, warfarins) via food or occupational accidents are difficult to diagnose. This article discusses different types of acquired vitamin K-dependent coagulopathy. Differential diagnosis is primarily based on patient statements before additional causes of vitamin K deficiency are explored. Even when pathological vitamin K deficiency is not determined, appropriate and urgent medical treatment is necessary: administration of fresh frozen plasma or concentrated factors of the prothrombin complex, administration of vitamin K remedies along with symptomatic therapy. With early diagnosis and prescription of appropriate therapy, prognosis is favorable. Reasons for vitamin K antagonists–dependent coagulopathy cases

ACS Style

Valery V. Wojciechowski; Daniela Calina; Konstantinos Tsarouhas; Alexander V. Pivnik; Alexander A. Sergievich; Vladimir V. Kodintsev; Ekaterina A. Filatova; Eren Ozcagli; Anca Oana Docea; Andreea Letitia Arsene; Eliza Gofita; Christina Tsitsimpikou; Aristidis M. Tsatsakis; Kirill S. Golokhvast. A guide to acquired vitamin K coagulophathy diagnosis and treatment: the Russian perspective. DARU Journal of Pharmaceutical Sciences 2017, 25, 1 -10.

AMA Style

Valery V. Wojciechowski, Daniela Calina, Konstantinos Tsarouhas, Alexander V. Pivnik, Alexander A. Sergievich, Vladimir V. Kodintsev, Ekaterina A. Filatova, Eren Ozcagli, Anca Oana Docea, Andreea Letitia Arsene, Eliza Gofita, Christina Tsitsimpikou, Aristidis M. Tsatsakis, Kirill S. Golokhvast. A guide to acquired vitamin K coagulophathy diagnosis and treatment: the Russian perspective. DARU Journal of Pharmaceutical Sciences. 2017; 25 (1):1-10.

Chicago/Turabian Style

Valery V. Wojciechowski; Daniela Calina; Konstantinos Tsarouhas; Alexander V. Pivnik; Alexander A. Sergievich; Vladimir V. Kodintsev; Ekaterina A. Filatova; Eren Ozcagli; Anca Oana Docea; Andreea Letitia Arsene; Eliza Gofita; Christina Tsitsimpikou; Aristidis M. Tsatsakis; Kirill S. Golokhvast. 2017. "A guide to acquired vitamin K coagulophathy diagnosis and treatment: the Russian perspective." DARU Journal of Pharmaceutical Sciences 25, no. 1: 1-10.

Journal article
Published: 15 December 2016 in Experimental and Therapeutic Medicine
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Anabolic androgenic steroids (AAS) are performance-enhancing drugs commonly abused by atheletes. Stanozolol is a synthetic testosterone-derived anabolic steroid. Although it is well known that AAS have several side-effects, there are only few toxicological studies available on the toxic effects and mechanisms of action of stanozolol. The aim of this study was to investigate the genotoxic effects of stanozolol and to determine its effects on telomerase activity in Sprague-Dawley male rats. For this purpose, 34 male rats were divided into 5 groups as follows: i) the control group (n=5); ii) the propylene glycol (PG)-treated group (n=5); iii) the stanozolol-treated group (n=8); iv) the PG-treated group subjected to exercise (n=8); and v) the stanozolol-treated group subjected to exercise (n=8). PG is used as a solvent control in our study. Stanozolol (5 mg/kg) and PG (1 ml/kg) were injected subcutaneously 5 days/week for 28 days. After 28 days, the animals were sacrificed, and DNA damage evaluation (comet assay) and telomerase activity assays were then performed using peripheral blood mononuclear cells (PBMCs). Telomerase activity was measured by using the TeloTAGGG Telomerase PCR ELISA PLUS kit. The results of this study revealed that stanozolol treatment induced DNA damage, while exercise exerted a protective effect. Stanozolol treatment without exercise stimulation was associated with a significant increase in telomerase activity in the PBMCs.

ACS Style

Mehtap Kara; Eren Ozcagli; Persefoni Fragkiadaki; Tuğba Kotil; Polychronis D. Stivaktakis; Demetrios A. Spandidos; Aristides M. Tsatsakis; Buket Alpertunga. Determination of DNA damage and telomerase activity in stanozolol-treated rats. Experimental and Therapeutic Medicine 2016, 13, 614 -618.

AMA Style

Mehtap Kara, Eren Ozcagli, Persefoni Fragkiadaki, Tuğba Kotil, Polychronis D. Stivaktakis, Demetrios A. Spandidos, Aristides M. Tsatsakis, Buket Alpertunga. Determination of DNA damage and telomerase activity in stanozolol-treated rats. Experimental and Therapeutic Medicine. 2016; 13 (2):614-618.

Chicago/Turabian Style

Mehtap Kara; Eren Ozcagli; Persefoni Fragkiadaki; Tuğba Kotil; Polychronis D. Stivaktakis; Demetrios A. Spandidos; Aristides M. Tsatsakis; Buket Alpertunga. 2016. "Determination of DNA damage and telomerase activity in stanozolol-treated rats." Experimental and Therapeutic Medicine 13, no. 2: 614-618.

Journal article
Published: 26 May 2016 in Cogent Food & Agriculture
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ACS Style

Abdurrahman Dundar; Veysi Okumus; Sadin Ozdemir; Kadir Serdar Celik; Eren Ozcagli. Determination of cytotoxic, anticholinesterase, antioxidant and antimicrobial activities of some wild mushroom species. Cogent Food & Agriculture 2016, 2, 1 .

AMA Style

Abdurrahman Dundar, Veysi Okumus, Sadin Ozdemir, Kadir Serdar Celik, Eren Ozcagli. Determination of cytotoxic, anticholinesterase, antioxidant and antimicrobial activities of some wild mushroom species. Cogent Food & Agriculture. 2016; 2 (1):1.

Chicago/Turabian Style

Abdurrahman Dundar; Veysi Okumus; Sadin Ozdemir; Kadir Serdar Celik; Eren Ozcagli. 2016. "Determination of cytotoxic, anticholinesterase, antioxidant and antimicrobial activities of some wild mushroom species." Cogent Food & Agriculture 2, no. 1: 1.

Journal article
Published: 01 April 2016 in Food and Chemical Toxicology
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Exposure to benzene promotes oxidative stress through the production of ROS, which can damage biological structures with the formation of new metabolites which can be used as markers of oxidant/antioxidant imbalance. This study aims to assess modifications in circulating levels of advanced oxidation protein products (AOPP), advanced glycation end-products (AGE) and serum reactive oxygen metabolites (ROMs) in a group of gasoline station attendants exposed to low-dose benzene and to evaluate the influence of antioxidant food intake on these biomarkers of oxidative stress. The diet adopted by the population examined consisted of compounds belonging to the classes of terpenoids, stilbenes and flavonoids, notably resveratrol, lycopene and apigenin. Ninety one gasoline station attendants occupationally exposed to benzene and 63 unexposed male office workers were recruited for this study. Urinary trans, trans-muconic acid (t,t-MA) concentration, determined to assess individual exposure level, resulted significantly higher in exposed workers. In subjects exposed to benzene, we observed a significant increase (p < 0.001) in ROMs and AOPP levels, which were also negatively correlated with fruit and vegetables consumption. By contrast, AGE did not show a significant increase and consequently any relation with antioxidant food intake. Only ROMs, representing a global biomarker of oxidative status, resulted correlated to t,t-MA levels (p < 0.01), probably due to low-dose exposure. Increase of ROS induced by reactive benzene metabolites may promote specific biochemical pathways with a major production of AOPP, which seem to represent a more sensitive biochemical marker of oxidative stress in workers exposed to benzene compared to AGE. Furthermore, this is the first study demonstrating ROMs increment in subject exposed to benzene. These biomarkers may be useful for screening purposes in gasoline station workers and other subjects exposed to low-dose benzene. Moreover, a diet rich in fruits and vegetables demonstrated an inverse association with the levels of oxidative stress markers, suggesting a protective role of antioxidant food intake in workers exposed to oxidant agents.

ACS Style

Chiara Costa; Eren Ozcagli; Silvia Gangemi; Federico Schembri; Federica Giambò; Vasilis Androutsopoulos; Aristidis Tsatsakis; Concettina Fenga. Molecular biomarkers of oxidative stress and role of dietary factors in gasoline station attendants. Food and Chemical Toxicology 2016, 90, 30 -35.

AMA Style

Chiara Costa, Eren Ozcagli, Silvia Gangemi, Federico Schembri, Federica Giambò, Vasilis Androutsopoulos, Aristidis Tsatsakis, Concettina Fenga. Molecular biomarkers of oxidative stress and role of dietary factors in gasoline station attendants. Food and Chemical Toxicology. 2016; 90 ():30-35.

Chicago/Turabian Style

Chiara Costa; Eren Ozcagli; Silvia Gangemi; Federico Schembri; Federica Giambò; Vasilis Androutsopoulos; Aristidis Tsatsakis; Concettina Fenga. 2016. "Molecular biomarkers of oxidative stress and role of dietary factors in gasoline station attendants." Food and Chemical Toxicology 90, no. : 30-35.

Journal article
Published: 01 March 2016 in Food and Chemical Toxicology
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3-monochloropropane-1,2-diol (3-MCPD) is a food contaminant that occurs during industrial production processes and can be found mainly in fat and salt containing products. 3-MCPD has exhibited mutagenic activity in vitro but not in vivo, however, a genotoxic mechanism for the occurrence of kidney tumors has not so far been excluded. The main pathway of mammalian 3-MCPD metabolism is via the formation of β--chlorolactatic acid and formation of glycidol has been demonstrated in bacterial metabolism. The aim of this study was to investigate genotoxic and oxidative DNA damaging effects of 3-MCPD and its metabolites, and to provide a better understanding of their roles in DNA repair processes. DNA damage was assessed by alkaline comet assay in target rat kidney epithelial cell lines (NRK-52E) and human embryonic kidney cells (HEK-293). Purine and pyrimidine base damage, H2O2 sensitivity and DNA repair capacity were assessed via modified comet assay. The results revealed in vitro evidence for increased genotoxicity and H2O2 sensitivity. No association was found between oxidative DNA damage and DNA repair capacity with the exception of glycidol treatment at 20 μg/mL. These findings provide further insights into the mechanisms underlying the in vitro genotoxic potential of 3-MCPD and metabolites.

ACS Style

Eren Ozcagli; Buket Alpertunga; Concettina Fenga; Mehmet Berktas; Christina Tsitsimpikou; Martin F. Wilks; Αristidis M. Tsatsakis. Effects of 3-monochloropropane-1,2-diol (3-MCPD) and its metabolites on DNA damage and repair under in vitro conditions. Food and Chemical Toxicology 2016, 89, 1 -7.

AMA Style

Eren Ozcagli, Buket Alpertunga, Concettina Fenga, Mehmet Berktas, Christina Tsitsimpikou, Martin F. Wilks, Αristidis M. Tsatsakis. Effects of 3-monochloropropane-1,2-diol (3-MCPD) and its metabolites on DNA damage and repair under in vitro conditions. Food and Chemical Toxicology. 2016; 89 ():1-7.

Chicago/Turabian Style

Eren Ozcagli; Buket Alpertunga; Concettina Fenga; Mehmet Berktas; Christina Tsitsimpikou; Martin F. Wilks; Αristidis M. Tsatsakis. 2016. "Effects of 3-monochloropropane-1,2-diol (3-MCPD) and its metabolites on DNA damage and repair under in vitro conditions." Food and Chemical Toxicology 89, no. : 1-7.

Journal article
Published: 05 February 2016 in Chemosphere
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The present in-vivo study focuses on the genotoxic effect of the neonicotinoid pesticide imidacloprid (IMI) in rabbits. The purpose of the study was to establish a possible relationship between exposure to the pesticide (dose and duration) and genotoxicity. Furthermore, an analytical method for the simultaneous determination of IMI and its major metabolite 6-chloronicotinic acid (6-ClNA) in blood was developed and validated. The isolation of the two analytes from blood was performed by liquid–liquid extraction with dichloromethane. Analysis was performed by Liquid Chromatography – Atmospheric Pressure Chemical Ionization – Mass Spectrometry (LC-APCI-MS). The method was applied on the determination of IMI and 6-ClNA in serum samples obtained from rabbits fed with the insecticide at two low doses. Furthermore, parameters of genotoxicity and cytotoxicity were evaluated by measuring binucleated cells with micronuclei (BNMN), micronuclei (MN) and the Cytokinesis Block Proliferation Index (CBPI), in lymphocytes of exposed rabbits. The results revealed a genotoxic effect of IMI for both exposed groups. There were statistically significant differences in the frequencies of BNMN and MN between control and exposed groups but there was no dose-dependence, neither time-dependence of the genotoxic effect for the administered doses. This is the first time that long term exposure to IMI in rabbits was studied for the determination of its genotoxic effect. The genotoxic effect of IMI as it is depicted by the current study is in accordance with previous studies.

ACS Style

Polychronis D. Stivaktakis; Matthaios P. Kavvalakis; Manolis N. Tzatzarakis; Athanasios Alegakis; Michael N. Panagiotakis; Persefoni Fragkiadaki; Elena Vakonaki; Eren Ozcagli; Wallace A. Hayes; V.N. Rakitskii; Aristidis M. Tsatsakis. Long-term exposure of rabbits to imidaclorpid as quantified in blood induces genotoxic effect. Chemosphere 2016, 149, 108 -113.

AMA Style

Polychronis D. Stivaktakis, Matthaios P. Kavvalakis, Manolis N. Tzatzarakis, Athanasios Alegakis, Michael N. Panagiotakis, Persefoni Fragkiadaki, Elena Vakonaki, Eren Ozcagli, Wallace A. Hayes, V.N. Rakitskii, Aristidis M. Tsatsakis. Long-term exposure of rabbits to imidaclorpid as quantified in blood induces genotoxic effect. Chemosphere. 2016; 149 ():108-113.

Chicago/Turabian Style

Polychronis D. Stivaktakis; Matthaios P. Kavvalakis; Manolis N. Tzatzarakis; Athanasios Alegakis; Michael N. Panagiotakis; Persefoni Fragkiadaki; Elena Vakonaki; Eren Ozcagli; Wallace A. Hayes; V.N. Rakitskii; Aristidis M. Tsatsakis. 2016. "Long-term exposure of rabbits to imidaclorpid as quantified in blood induces genotoxic effect." Chemosphere 149, no. : 108-113.

Journal article
Published: 01 January 2016 in Toxicology Letters
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Abuse of anabolic androgenic steroids is linked to a variety of cardiovascular complications. The aim of our study was to investigate the possible cardiovascular effects of nandrolone decanoate on young rabbits using echocardiography, histology and monitoring of telomerase activity, oxidative stress and biochemical markers. Fourteen rabbits were divided into three administration groups and the control group. Doses of 4mg/kg and 10mg/kg of nandrolone decanoate, given intramuscularly and subcutaneously, two days per week for six months were applied. A 4-months wash-out period followed. Focal fibrosis and inflammatory infiltrations of cardiac tissue were observed in the high dose groups. Thiobarbituric acid-reactive species (TBARS) levels were significantly increased in the high dose groups, while catalase activity decreased. Myocardial Performance Index (MPI) is the main echocardiographic index primarily affected by nandrolone administration in rabbits. Despite the preserved systolic performance, histological lesions observed associated with distorted MPI values, point to diastolic impairment of the thickened myocardium due to nandrolone treatment. Oxidative stress accumulates and telomerase activity in cardiac tissue rises. Subcutaneous administration seems to be more deleterious to the cardiovascular system, as oxidative stress, telomerase activity and biochemical markers do not appear to return into normal values in the wash-out period.

ACS Style

Fotini Vasilaki; Christina Tsitsimpikou; Konstantinos Tsarouhas; Ioannis Germanakis; Marias Tzardi; Matthaios Kavvalakis; Eren Ozcagli; Dimitrios Kouretas; Aristidis M. Tsatsakis. Cardiotoxicity in rabbits after long-term nandrolone decanoate administration. Toxicology Letters 2016, 241, 143 -151.

AMA Style

Fotini Vasilaki, Christina Tsitsimpikou, Konstantinos Tsarouhas, Ioannis Germanakis, Marias Tzardi, Matthaios Kavvalakis, Eren Ozcagli, Dimitrios Kouretas, Aristidis M. Tsatsakis. Cardiotoxicity in rabbits after long-term nandrolone decanoate administration. Toxicology Letters. 2016; 241 ():143-151.

Chicago/Turabian Style

Fotini Vasilaki; Christina Tsitsimpikou; Konstantinos Tsarouhas; Ioannis Germanakis; Marias Tzardi; Matthaios Kavvalakis; Eren Ozcagli; Dimitrios Kouretas; Aristidis M. Tsatsakis. 2016. "Cardiotoxicity in rabbits after long-term nandrolone decanoate administration." Toxicology Letters 241, no. : 143-151.

Journal article
Published: 01 January 2015 in Pharmaceutica Analytica Acta
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Eren Ozcagli, Buket Alpertunga and Aristidis M Tsatsakis-Biosimilar Drugs and Pharmacovigilance

ACS Style

Eren Ozcagli Buket Alpertunga; Aristidis M Tsatsakis. Biosimilar Drugs and Pharmacovigilance. Pharmaceutica Analytica Acta 2015, 06, 1 .

AMA Style

Eren Ozcagli Buket Alpertunga, Aristidis M Tsatsakis. Biosimilar Drugs and Pharmacovigilance. Pharmaceutica Analytica Acta. 2015; 06 (09):1.

Chicago/Turabian Style

Eren Ozcagli Buket Alpertunga; Aristidis M Tsatsakis. 2015. "Biosimilar Drugs and Pharmacovigilance." Pharmaceutica Analytica Acta 06, no. 09: 1.

Journal article
Published: 01 January 2015 in Food and Chemical Toxicology
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The occurrence of residues of DDT and its metabolites was monitored in 196 cow milk samples of various pasteurized commercial types collected from the Greek market. Residue levels were determined by GC-MS analysis. In 97.4% of the samples at least one DDT isomer or one of the DDT metabolites was detected, in levels not exceeding the maximum permitted residue level by the EU. Hazard Index for both carcinogenic and non-carcinogenic effects was estimated under two assumptions: a) using DDT concentrations from positive samples and b) imputing LOD/2 as an arbitrary concentration for negative samples. No statistically significant differences in detected or summed residue (p > 0.05) concentrations between different milk types were observed, with the exception of specific metabolites of DDT in some milk types. Exposure assessment scenarios were developed for children aged 1, 3, 5, 7 and 12 years old based on estimated body weights and daily milk consumption. Hazard Indices for non-carcinogenic effects were below 0.109 covering also carcinogenic effects according to WHO approach. The cancer risk values for carcinogenic effects according to the US EPA Cancer Benchmark Concentration approach, ranged from 0.4 to 18. For both effects the highest values were calculated for the 1- to 3-year-old age groups.

ACS Style

Ioannis N. Tsakiris; Marina Goumenou; Manolis N. Tzatzarakis; Athanasios Alegakis; Christina Tsitsimpikou; Eren Ozcagli; Dionysios Vynias; Aristidis M. Tsatsakis. Risk assessment for children exposed to DDT residues in various milk types from the Greek market. Food and Chemical Toxicology 2015, 75, 156 -165.

AMA Style

Ioannis N. Tsakiris, Marina Goumenou, Manolis N. Tzatzarakis, Athanasios Alegakis, Christina Tsitsimpikou, Eren Ozcagli, Dionysios Vynias, Aristidis M. Tsatsakis. Risk assessment for children exposed to DDT residues in various milk types from the Greek market. Food and Chemical Toxicology. 2015; 75 ():156-165.

Chicago/Turabian Style

Ioannis N. Tsakiris; Marina Goumenou; Manolis N. Tzatzarakis; Athanasios Alegakis; Christina Tsitsimpikou; Eren Ozcagli; Dionysios Vynias; Aristidis M. Tsatsakis. 2015. "Risk assessment for children exposed to DDT residues in various milk types from the Greek market." Food and Chemical Toxicology 75, no. : 156-165.

Journal article
Published: 01 January 2015 in Journal of Horticulture
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The study focused to evaluate cytotoxic, antioxidant, antimicrobial and anticholinesterase activities of methanol extracts of Pleurotus ostreatus Jacq. (Pleurotaceae), Boletus edulis Bull. (Boletaceae), Tricholoma populinum J. (Tricholomataceae) Helvella queletii Bres. (Helvellaceae), Armillaria tabescens Emel. (Physalacriaceae), Psathyrella candolleana Fr. (Psathyrellaceae) and Helvella leucopus Pers. (Helvellaceae) mushroom species. Phenolic acid profiles of these mushrooms were also determined to obtain further information on the correlation between the contents of phenolic compounds and studied activities. Cytotoxic activity of mushrooms was screened by MTT cytotoxicity assay on cancer (HeLa) and normal epithelium (NRK-52E) cell lines. To determine antioxidant potential of mushroom extracts free radical scavenging, reducing power, superoxide anion radical scavenging, total antioxidant and metal chelating activities were studied, To indicate anthicholinesterase activity the acetyl-and butyrylcholinesterase inhibitory activities of the mushroom extracts were studied. For antimicrobial activity disc diffusion method was applied. Phenolic profile of mushrooms were determined by HPLC system. The IC50 values of the extracts were 1.58-25.11 and 2.05-22.32 mg/mL for HeLa and NRK-52E cells, respectively. At antimicrobial activity the inhibiton zones were found to be as 1 ± 0.12-13 ± 0.23 mm. P. ostreatus, B. edulis and H. leucopus extracts were showed higher activities than the other mushroms at antioxidant, antimicrobial, anticholinesterase and cytotoxic activity.

ACS Style

Veysi Okumus Abdurrahman Dundar; Veysi Okumus; Sadin Ozdemir; Kadir Serdar Celik; Mehmet Boga; Eren Ozcagli; Gul Ozhan; Abdunnasir Yildiz.. Antioxidant, Antimicrobial, Cytotoxic and Anticholinesterase Activities of Seven Mushroom Species with their Phenolic Acid Composition. Journal of Horticulture 2015, 2, 1 .

AMA Style

Veysi Okumus Abdurrahman Dundar, Veysi Okumus, Sadin Ozdemir, Kadir Serdar Celik, Mehmet Boga, Eren Ozcagli, Gul Ozhan, Abdunnasir Yildiz.. Antioxidant, Antimicrobial, Cytotoxic and Anticholinesterase Activities of Seven Mushroom Species with their Phenolic Acid Composition. Journal of Horticulture. 2015; 2 (4):1.

Chicago/Turabian Style

Veysi Okumus Abdurrahman Dundar; Veysi Okumus; Sadin Ozdemir; Kadir Serdar Celik; Mehmet Boga; Eren Ozcagli; Gul Ozhan; Abdunnasir Yildiz.. 2015. "Antioxidant, Antimicrobial, Cytotoxic and Anticholinesterase Activities of Seven Mushroom Species with their Phenolic Acid Composition." Journal of Horticulture 2, no. 4: 1.

Journal article
Published: 01 September 2014 in Toxicology Letters
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Eren Ozcagli; Buket Alpertunga. Evaluation of DNA damage in NRK 52E cells following in vitro treatment of 3-MCPD and its metabolites. Toxicology Letters 2014, 229, S180 .

AMA Style

Eren Ozcagli, Buket Alpertunga. Evaluation of DNA damage in NRK 52E cells following in vitro treatment of 3-MCPD and its metabolites. Toxicology Letters. 2014; 229 ():S180.

Chicago/Turabian Style

Eren Ozcagli; Buket Alpertunga. 2014. "Evaluation of DNA damage in NRK 52E cells following in vitro treatment of 3-MCPD and its metabolites." Toxicology Letters 229, no. : S180.

Journal article
Published: 01 September 2014 in Toxicology Letters
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ACS Style

Fotini Vasilaki; Christina Tsitsimpikou; Konstantinos Tsarouhas; Eren Ozcagli; Persefoni Fragkiadaki; Ioannis Heretis; Leda Kovatsi; Michael Kyriakakis; Aristidis Tsatsakis. Biochemical markers in long-term exposed rabbits to nandrolone decanoate. Toxicology Letters 2014, 229, S217 .

AMA Style

Fotini Vasilaki, Christina Tsitsimpikou, Konstantinos Tsarouhas, Eren Ozcagli, Persefoni Fragkiadaki, Ioannis Heretis, Leda Kovatsi, Michael Kyriakakis, Aristidis Tsatsakis. Biochemical markers in long-term exposed rabbits to nandrolone decanoate. Toxicology Letters. 2014; 229 ():S217.

Chicago/Turabian Style

Fotini Vasilaki; Christina Tsitsimpikou; Konstantinos Tsarouhas; Eren Ozcagli; Persefoni Fragkiadaki; Ioannis Heretis; Leda Kovatsi; Michael Kyriakakis; Aristidis Tsatsakis. 2014. "Biochemical markers in long-term exposed rabbits to nandrolone decanoate." Toxicology Letters 229, no. : S217.