Three-dimensional printing is an additive manufacturing process that allows rapid design and manufacture of complex component based on computer-aided design models. Compared with some conventional manufacturing processes, additive manufacturing part properties can depend on structural and process parameters rather than purely on material properties. The objectives of the paper are to evaluate the tensile properties of 3D printed components produced using a commercial 3D printer by performing standard tensile tests and to assess the influence of the technological parameters upon mechanical proprieties of printed specimens, considering different printing directions, infill rates and infill patterns. The influence of raster angles is tested through the designed specimens with different transverse plane, they are printed by placing in different angle, including 0°, 30°, 45° and 90°. Specimens with an infill rate varying from 20% to 100% and six different infill patterns has been tested.

Bacterial cellulose (BC) is a natural polymer with properties suitable for tissue engineering and possible applications in scaffold production. However, current procedures have limitations in obtaining BC pellicles with the desired structural, physical, and mechanical properties. Thus, this study analyzed the optimal culture conditions of BC membranes and two types of processing: draining and oven-drying. The aim was to obtain BC membranes with properties suitable for a wound dressing material. Two studies were carried out. In the preliminary study, the medium (100 mL) was inoculated with varying volumes (1, 2, 3, 4, and 5 mL) and incubated statically for different periods (3, 6, 9, 12, and 18 days), using a full factorial experimental design. Thickness, uniformity, weight, and yield were evaluated. In the optimization study, a Box–Behnken design was used. Two independent variables were used: inoculum volume (X₁: 1, 3, and 5 mL) and fermentation period (X₂: 6, 12, and 18 d) to determine the target response variables: thickness, swelling ratio, drug release, fiber diameter, tensile strength, and Young’s modulus for both dry and moist BC membranes. The mathematical modelling of the effect of the two independent variables was performed by response surface methodology (RSM). The obtained models were validated with new experimental values and confirmed for all tested properties, except Young’s modulus of oven-dried BC. Thus, the optimal properties in terms of a scaffold material of the moist BC were obtained with an inoculum volume of 5% (v/v) and 16 d of fermentation. While, for the oven-dried membranes, optimal properties were obtained with a 4% (v/v) and 14 d of fermentation.

The use of bacterial cellulose (BC) as scaffold for active biofilms is one of the most interesting applications, especially for the biomedical and food industries. However, there are currently few studies evaluating the potential of incorporating herbal extracts into various biomaterials, including BC. Thus, the aim of this study is to report a screening of the total phenolic content and antioxidant and antimicrobial activity of ethanolic extracts of oregano, rosemary, parsley, and lovage. At the same time, the bioactive potential of BC enriched with the four ethanolic extracts is described. Microwave-assisted extraction was used to extract bioactive compounds from the four selected herbs. The physical, mechanical, structural, and chemical properties of BC were also assessed. Next, BC was enriched with the extracts, and their effect against Escherichia coli, Staphylococcus aureus, and Candida albicans was evaluated. The results showed that the bioactivity of the herbs varied significantly, with rosemary extract being the most bioactive. The BC films possessed good mechanical properties, and a three-dimensional network fibrillar structure appropriate for ethanolic-extract incorporation. The BC samples enriched with rosemary extracts had the highest antibacterial activity against S. aureus, while E. coli. and C. albicans seemed to be resistant to all extracts, regardless of herbs.

Purpose: – The purpose of this paper was to present how customized implants could be made with specific properties, by setting different values of the laser power, within the selective laser melting (SLM) process. A detailed case study was undertaken and a new multi-structured femoral prosthesis was designed and analyzed, to simulate its behavior for a specific case study. Design/methodology/approach: – The materials and manufacturing methods are presented, with details regarding the SLM process, using the Realizer 250 machine. The laser power was varied between 50 and 200 W, thus obtaining samples with different physical and mechanical characteristics. All those sample parts were characterized and their properties were measured. Findings: – A practical methodology was found to produce multi-structured implants by SLM. Significant changes of the porosity and properties were found, when modifying the laser power at the SLM machine. The studies have indicated an open porosity varying between 24.810.83 per cent. Tensile tests of the samples showed Young’s modulus values varying between 13.5 and 104.5 GPa and an ultimate stress between 20.2 and 497.5 MPa. Research limitations/implications: – There is no Additive Manufacturing (AM) machine available, to work with different laser power values, in different regions of the same section of the implant. Hence, a multi-structured implant cannot be obtained directly. Practical implications: – The prosthesis should be specifically designed to contain separate models/regions to be made with appropriate laser power values. Originality/value: – This paper presents a new method to design and manufacture a multi-structured implant, using the existing AM equipment. A detailed case study is presented, showing the design procedure, the way to simulate its behavior and the methods to produce the implants by SLM.