The present study was conducted to examine that impact of dietary xylanase (Xyl) and arabinofuranosidase (Abf) supplementation on the performance, protein and fat digestibility, the lipid peroxidation, the plasma biochemical traits, and the immune response of broilers. A total of 480, un-sexed, and one-day-old broilers (Ross 308) were randomly divided into three treatments with eight replicates, where chicks in the first treatment were fed basal diets and served as the control, chicks in the second treatment were fed diets formulated with reductions of 90 kcal/kg, and chicks in the third treatment were fed the same formulated diets used in the second group as well as the Xyl and Abf combination (Rovabio^® Advance). Feed intake was decreased by the low energy diet, leading to an enhancement in feed efficiency enzyme supplementation in the low energy diet (p < 0.015). Both protein and fat digestibility were improved (p < 0.047) due to enzyme supplementation. Moreover, enzyme supplementation increased muscle total lipids content and decreased muscle thiobarbituric acid retroactive substance content. Furthermore, diets supplemented with Xyl and Abf exhibited an increase in antibody titers against the Newcastle disease virus (p < 0.026). In addition, enzyme supplementation increased gene expression related to growth and gene expression related to fatty acid synthesis. It could be concluded that dietary Xyl and Abf supplementation had beneficial impacts on growth, nutrient digestibility, lipid peroxidation, immune response, and gene expressions related to growth and fatty acid synthesis in broiler chickens fed low-energy diets.

The objective of this study was to examine the effects of diets supplemented with corn silk meal (CSM) and non-starch polysaccharide (NSP) enzyme on growth performance, blood biochemistry, immunological response, and growth-related gene expression in broiler chickens. A total of 270 broiler chickens were divided into six experimental groups: (1) basal diets (BD) as control; (2) BD supplemented with 0.5 g/kg feed NSP enzyme; the other four groups are CSM diets as following; (3) and (4) fed diet contain 40 and 80 kg/ton of CSM; (5) and (6) fed diet contain 40 and 80 kg/ton CSM and supplemented with 0.5 g/kg NSP enzyme. Body weight gain (BWG), feed conversion ratio (FCR), protein retention and fiber digestibility were synergistically improved (p < 0.05) when fed CSM supplemented with NSP enzyme. Moreover, a synergistic decrease (p < 0.05) in the serum glucose and total cholesterol were found. Immune organ weights and Newcastle disease virus titers were increased with CSM diets. Interestingly, the relative mRNAs of the growth hormone receptor (GHR) and insulin growth factor (IGF) were increased (p < 0.05) with the CSM and NSP enzyme: the relative mRNA expressions of cholecystokinin (CCK) and leptin were decreased by feeding CSM diets with the NSP enzyme. It could be concluded that the dietary inclusion of CSM with the NSP enzyme might improve growth performance, modify plasma lipids, and enhance immune response in broilers.

This experiment was designed to evaluate the impact of dietary supplementation with copper oxide nanoparticles (CuO_NPs) on growth, serum biochemical and lipid profiles, and relative immune organs weights of Ross 308 and Cobb 500 broilers exposed to chronic heat stress (HS). Chicks were allotted in three treatments with three replicates per each treatment. Chicks in the 1st treatment group fed 100 % of its recommended Cu needs in the form of CuO while, 2nd & 3rd groups received 100 % and 50% of their Cu needs as CuO_NPs, respectively. At 21 days old, each group was split into two, normal (housed at 24 °C) and HS (exposed to 33±2°C for 5 h/day for 2 weeks). CuO_NPs supplementation to Cobb and Ross broilers significantly modified their growth. Furthermore, it altered their serum globulin concentration while non-significant improvement in liver function indicators was recorded. In addition, dietary inclusion of CuO_NPs for the two studied strains significantly modified their serum ALP levels and significantly modified their abdominal fat content and serum lipid profile (cholesterol, triglycerides, HDL and LDL). In conclusion, dietary supplementation of CuO_NPs to Cobb and Ross birds reared under HS conditions can effectively improve growth performance and biochemical profile, reduce serum cholesterol content, and restored the relative weight of the bursa, thymus, and spleen.