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Agnieszka Rogowska
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarina 7, 87-100 Torun, Poland

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Journal article
Published: 20 May 2021 in International Journal of Molecular Sciences
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The most critical group of all includes multidrug resistant bacteria that pose a particular threat in hospitals, as they can cause severe and often deadly infections. Modern medicine still faces the difficult task of developing new agents for the effective control of bacterial-based diseases. The targeted administration of nanoparticles can enhance the efficiency of conventional pharmaceutical agents. However, the interpretation of interfaces’ interactions between nanoparticles and biological systems still remains a challenge for researchers. In fact, the current research presents a strategy for using ZnO NPs immobilization with ampicillin and tetracycline. Firstly, the study provides the mechanism of the ampicillin and tetracycline binding on the surface of ZnO NPs. Secondly, it examines the effect of non-immobilized ZnO NPs, immobilized with ampicillin (ZnONPs/AMP) and tetracycline (ZnONPs/TET), on the cells’ metabolism and morphology, based on the protein and lipid profiles. A sorption kinetics study showed that the antibiotics binding on the surface of ZnONPs depend on their structure. The efficiency of the process was definitely higher in the case of ampicillin. In addition, flow cytometry results showed that immobilized nanoparticles present a different mechanism of action. Moreover, according to the MALDI approach, the antibacterial activity mechanism of the investigated ZnO complexes is mainly based on the destruction of cell membrane integrity by lipids and proteins, which is necessary for proper cell function. Additionally, it was noticed that some of the identified changes indicate the activation of defense mechanisms by cells, leading to a decrease in the permeability of a cell’s external barriers or the synthesis of repair proteins.

ACS Style

Agnieszka Rogowska; Viorica Railean-Plugaru; Paweł Pomastowski; Justyna Walczak-Skierska; Anna Król-Górniak; Adrian Gołębiowski; Bogusław Buszewski. The Study on Molecular Profile Changes of Pathogens via Zinc Nanocomposites Immobilization Approach. International Journal of Molecular Sciences 2021, 22, 5395 .

AMA Style

Agnieszka Rogowska, Viorica Railean-Plugaru, Paweł Pomastowski, Justyna Walczak-Skierska, Anna Król-Górniak, Adrian Gołębiowski, Bogusław Buszewski. The Study on Molecular Profile Changes of Pathogens via Zinc Nanocomposites Immobilization Approach. International Journal of Molecular Sciences. 2021; 22 (10):5395.

Chicago/Turabian Style

Agnieszka Rogowska; Viorica Railean-Plugaru; Paweł Pomastowski; Justyna Walczak-Skierska; Anna Król-Górniak; Adrian Gołębiowski; Bogusław Buszewski. 2021. "The Study on Molecular Profile Changes of Pathogens via Zinc Nanocomposites Immobilization Approach." International Journal of Molecular Sciences 22, no. 10: 5395.

Journal article
Published: 23 May 2020 in Materials
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The application of silver nanoparticles as an antibacterial agent is becoming more common. Unfortunately, their effect on microorganisms is still not fully understood. Therefore, this paper attempts to investigate the influence of silver ions, biologically synthesized silver nanoparticles and nanoparticles functionalized with antibiotics on molecular bacteria profiles. The initial stage of research was aimed at the mechanism determination involved in antibiotics sorption onto nanoparticles’ surface. For this purpose, the kinetics study was performed. Next, the functionalized formulations were characterized by Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS) and a zeta potential study. The results reveal that functionalization is a complex process, but does not significantly affect the stability of biocolloids. Furthermore, the antimicrobial assays, in most cases, have shown no increases in antibacterial activity after nanoparticle functionalization, which suggests that the functionalization process does not always generate the improved antimicrobial effect. Finally, the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique was employed to characterize the changes in the molecular profile of bacteria treated with various antibacterial agents. The recorded spectra proved many differences in bacterial lipids and proteins profiles compared to untreated cells. In addition, the statistical analysis of recorded spectra revealed the strain-dependent nature of stress factors on the molecular profile of microorganisms.

ACS Style

Bogusław Buszewski; Agnieszka Rogowska; Viorica Railean-Plugaru; Michał Złoch; Justyna Walczak-Skierska; Paweł Pomastowski. The Influence of Different Forms of Silver on Selected Pathogenic Bacteria. Materials 2020, 13, 2403 .

AMA Style

Bogusław Buszewski, Agnieszka Rogowska, Viorica Railean-Plugaru, Michał Złoch, Justyna Walczak-Skierska, Paweł Pomastowski. The Influence of Different Forms of Silver on Selected Pathogenic Bacteria. Materials. 2020; 13 (10):2403.

Chicago/Turabian Style

Bogusław Buszewski; Agnieszka Rogowska; Viorica Railean-Plugaru; Michał Złoch; Justyna Walczak-Skierska; Paweł Pomastowski. 2020. "The Influence of Different Forms of Silver on Selected Pathogenic Bacteria." Materials 13, no. 10: 2403.

Journal article
Published: 08 August 2019 in International Journal of Molecular Sciences
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The work presents the kinetic and isotherm studies of silver binding on casein, which was carried out using batch sorption technique. Moreover, the influence of light irradiation on the process was shown. In order to investigate the mechanism of metal ions sorption by casein the zero, pseudo-first order kinetics and Weber-Morris intra-particle diffusion as well as Langmuir and Freundlich isotherm models were used. Furthermore, to specify more precisely, the possible binding mechanism, the spectroscopic (FT-IR—Fourier Transform Infrared Spectroscopy, Raman), spectrometric (MALDI-TOF MS—Matrix-Assisted Laser Desorption/Ionization Time Of Flight Mass Spectrometry), microscopic (SEM—Scanning Electron Microscope, TEM/EDX—Transmission Electron Microscopy/Energy Dispersive X-ray detector) and thermal (TGA—Thermogravimetric Analysis, DTG—Derivative Thermogravimetry) analysis were performed. Kinetic study indicates that silver binding onto casein is a heterogeneous process with two main stages: initial rapid stage related to surface adsorption onto casein with immediate creation of silver nanoparticles and slower second stage of intraglobular diffusion with silver binding in chelated form (metalloproteins) or ion-exchange form. Spectroscopic techniques confirmed the binding process and MALDI-TOF MS analysis show the dominant contribution of the α-casein in the process. Moreover, the treatment of silver-casein complex by artificial physiological fluids was performed.

ACS Style

Oleksandra Pryshchepa; Gulyaim N. Sagandykova; Paweł Pomastowski; Viorica Railean-Plugaru; Anna Król; Agnieszka Rogowska; Agnieszka Rodzik; Myroslav Sprynskyy; Bogusław Buszewski. A New Approach for Spontaneous Silver Ions Immobilization onto Casein. International Journal of Molecular Sciences 2019, 20, 3864 .

AMA Style

Oleksandra Pryshchepa, Gulyaim N. Sagandykova, Paweł Pomastowski, Viorica Railean-Plugaru, Anna Król, Agnieszka Rogowska, Agnieszka Rodzik, Myroslav Sprynskyy, Bogusław Buszewski. A New Approach for Spontaneous Silver Ions Immobilization onto Casein. International Journal of Molecular Sciences. 2019; 20 (16):3864.

Chicago/Turabian Style

Oleksandra Pryshchepa; Gulyaim N. Sagandykova; Paweł Pomastowski; Viorica Railean-Plugaru; Anna Król; Agnieszka Rogowska; Agnieszka Rodzik; Myroslav Sprynskyy; Bogusław Buszewski. 2019. "A New Approach for Spontaneous Silver Ions Immobilization onto Casein." International Journal of Molecular Sciences 20, no. 16: 3864.

Journal article
Published: 07 August 2019 in Toxins
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The zearalenone binding and metabolization ability of probiotic microorganisms, such as lactic acid bacteria, Lactobacillus paracasei, Lactococcus lactis, and yeast Saccharomyces cerevisiae, isolated from food products, were examined. Moreover, the influence of cellular stress (induced by silver nanoparticles) and lyophilization on the effectiveness of tested microorganisms was also investigated. The concentration of zearalenone after a certain time of incubation with microorganisms was determined using high-performance liquid chromatography. The maximum sorption effectiveness for L. paracasei, L. lactis, and S. cerevisiae cultured in non-stress conditions was 53.3, 41.0, and 36.5%, respectively. At the same time for the same microorganisms cultured at cellular stress conditions, the maximum sorption effectiveness was improved to 55.3, 47.4, and 57.0%, respectively. Also, the effect of culture conditions on the morphology of the cells and its metabolism was examined using microscopic technique and matrix-assisted laser desorption ionization-time of flight mass spectrometry, respectively.

ACS Style

Agnieszka Rogowska; Paweł Pomastowski; Justyna Walczak; Viorica Railean-Plugaru; Joanna Rudnicka; Bogusław Buszewski. Investigation of Zearalenone Adsorption and Biotransformation by Microorganisms Cultured under Cellular Stress Conditions. Toxins 2019, 11, 463 .

AMA Style

Agnieszka Rogowska, Paweł Pomastowski, Justyna Walczak, Viorica Railean-Plugaru, Joanna Rudnicka, Bogusław Buszewski. Investigation of Zearalenone Adsorption and Biotransformation by Microorganisms Cultured under Cellular Stress Conditions. Toxins. 2019; 11 (8):463.

Chicago/Turabian Style

Agnieszka Rogowska; Paweł Pomastowski; Justyna Walczak; Viorica Railean-Plugaru; Joanna Rudnicka; Bogusław Buszewski. 2019. "Investigation of Zearalenone Adsorption and Biotransformation by Microorganisms Cultured under Cellular Stress Conditions." Toxins 11, no. 8: 463.