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Matteo Daghio
Dipartimento di Scienze e Tecnologie Agrarie, Alimentari, Ambientali e Forestali, University of Florence, Piazzale delle Cascine 18, 50144 Florence, Italy

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Review
Published: 20 August 2021 in Antibiotics
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For decades antibiotics have been used in poultry rearing to support high levels of production. Nevertheless, several problems have arisen because of the misuse of antibiotics (i.e., antibiotic resistance, residues in animal products, environmental pollution). Thus, the European Union (EU) as well as the European Food Safety Authority (EFSA) promote action plans to diminish the use of antibiotics in animal production. Alternatives to antibiotics have been studied. Polyphenols (PPs) or organic acids (OAs) seem to be two accredited solutions. Phenolic compounds, such as phenols, flavonoids, and tannins exert their antimicrobial effect with specific mechanisms. In contrast, short chain fatty acids (SCFAs) and medium chain fatty acids (MCFAs), the OAs mainly used as antibiotics alternative, act on the pathogens depending on the pKa value. This review aims to collect the literature reporting the effects of these substances applied as antimicrobial molecules or growth promoter in poultry feeding (both for broilers and laying hens). Organic acids and PPs can be used individually or in blends, exploiting the properties of each component. Collected data highlighted that further research needs to focus on OAs in laying hens’ feeding and also determine the right combination in blends with PPs.

ACS Style

Federica Scicutella; Federica Mannelli; Matteo Daghio; Carlo Viti; Arianna Buccioni. Polyphenols and Organic Acids as Alternatives to Antimicrobials in Poultry Rearing: A Review. Antibiotics 2021, 10, 1010 .

AMA Style

Federica Scicutella, Federica Mannelli, Matteo Daghio, Carlo Viti, Arianna Buccioni. Polyphenols and Organic Acids as Alternatives to Antimicrobials in Poultry Rearing: A Review. Antibiotics. 2021; 10 (8):1010.

Chicago/Turabian Style

Federica Scicutella; Federica Mannelli; Matteo Daghio; Carlo Viti; Arianna Buccioni. 2021. "Polyphenols and Organic Acids as Alternatives to Antimicrobials in Poultry Rearing: A Review." Antibiotics 10, no. 8: 1010.

Journal article
Published: 01 April 2021 in Sustainability
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In the past 10 years, the average demand for meat and milk across the world has significantly increased, especially in developing countries. Therefore, to support the production of animal-derived food products, a huge quantity of feed resources is needed. This paper does not present original research, but rather provides a conceptual strategy to improve primary production in a sustainable way, in relation to forthcoming issues linked to climate change. Increases in meat and milk production could be achieved by formulating balanced diets for ovines based on alfalfa integrated with local agricultural by-products. As the central component of the diet is alfalfa, one goal of the project is increasing the yield of alfalfa in a sustainable way via inoculating seeds with symbiotic rhizobia (i.e., Sinorhizobium meliloti). Seed inoculants are already present on the market but have not been optimized for arid soils. Furthermore, a part of the project is focused on the selection of elite symbiotic strains that show increased resistance to salt stress and competitiveness. The second component of the experimental diets is bio-waste, especially that obtained from olive oil manufacturing (i.e., pomace). The addition of agro-by-products allows us to use such waste as a resource for animal feeding, and possibly, to modulate rumen metabolism, thereby increasing the nutritional quality of milk and meat.

ACS Style

Carlo Viti; Agnese Bellabarba; Matteo Daghio; Alessio Mengoni; Marcello Mele; Arianna Buccioni; Gaio Pacini; Abdelkader Bekki; Khalid Azim; Majida Hafidi; Francesco Pini. Alfalfa for a Sustainable Ovine Farming System: Proposed Research for a New Feeding Strategy Based on Alfalfa and Ecological Leftovers in Drought Conditions. Sustainability 2021, 13, 3880 .

AMA Style

Carlo Viti, Agnese Bellabarba, Matteo Daghio, Alessio Mengoni, Marcello Mele, Arianna Buccioni, Gaio Pacini, Abdelkader Bekki, Khalid Azim, Majida Hafidi, Francesco Pini. Alfalfa for a Sustainable Ovine Farming System: Proposed Research for a New Feeding Strategy Based on Alfalfa and Ecological Leftovers in Drought Conditions. Sustainability. 2021; 13 (7):3880.

Chicago/Turabian Style

Carlo Viti; Agnese Bellabarba; Matteo Daghio; Alessio Mengoni; Marcello Mele; Arianna Buccioni; Gaio Pacini; Abdelkader Bekki; Khalid Azim; Majida Hafidi; Francesco Pini. 2021. "Alfalfa for a Sustainable Ovine Farming System: Proposed Research for a New Feeding Strategy Based on Alfalfa and Ecological Leftovers in Drought Conditions." Sustainability 13, no. 7: 3880.

Journal article
Published: 01 July 2020 in Sustainability
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The Food and Agriculture Organization’s previsions show that by 2050 the world’s population will reach 9.6 billion people, and the request for a high value protein source will increase as well. Poultry can guarantee high value protein for humans, even in the poorest regions of the world. Hence, efficient poultry production is needed, matching with sustainable development. The residual meal from cardoon seed oil (used for biodiesel and biodegradable bioplastic production) is suitable for animal feeding due to its protein content. The aim of this preliminary study was to test for a possible use of cardoon meal as a protein source in a poultry diet during the finishing period. Forty-five Kabir chickens were divided into three groups and fed three diets in which soybean meal (control) was partially (16%) or completely replaced with cardoon meal as a protein source (treated groups). In vivo performances, animal welfare, dressing out and meat color were evaluated. No statistical differences in feed efficiency, dressing out, nor in meat quality were found among groups. Moreover, birds that were fed cardoon meal showed lower perivisceral fat. Therefore, cardoon meal could be considered as an alternative for soybean meal in the finishing period in poultry feeding.

ACS Style

Arianna Buccioni; Giovanni Brajon; Lapo Nannucci; Vincenzo Ferrulli; Federica Mannelli; Antonino Barone; Matteo Daghio; Giulia Secci; Stefano Rapaccini; Domenico Gatta; Michele Falce; Sara Minieri. Cardoon Meal (Cynara cardunculus var. altilis) as Alternative Protein Source during Finishing Period in Poultry Feeding. Sustainability 2020, 12, 5336 .

AMA Style

Arianna Buccioni, Giovanni Brajon, Lapo Nannucci, Vincenzo Ferrulli, Federica Mannelli, Antonino Barone, Matteo Daghio, Giulia Secci, Stefano Rapaccini, Domenico Gatta, Michele Falce, Sara Minieri. Cardoon Meal (Cynara cardunculus var. altilis) as Alternative Protein Source during Finishing Period in Poultry Feeding. Sustainability. 2020; 12 (13):5336.

Chicago/Turabian Style

Arianna Buccioni; Giovanni Brajon; Lapo Nannucci; Vincenzo Ferrulli; Federica Mannelli; Antonino Barone; Matteo Daghio; Giulia Secci; Stefano Rapaccini; Domenico Gatta; Michele Falce; Sara Minieri. 2020. "Cardoon Meal (Cynara cardunculus var. altilis) as Alternative Protein Source during Finishing Period in Poultry Feeding." Sustainability 12, no. 13: 5336.

Journal article
Published: 10 February 2020 in Water
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Groundwater is the environmental matrix that is most frequently affected by anthropogenic hexavalent chromium contamination. Due to its carcinogenicity, Cr(VI) has to be removed, using environmental-friendly and economically sustainable remediation technologies. BioElectrochemical Systems (BESs), applied to bioremediation, thereby offering a promising alternative to traditional bioremediation techniques, without affecting the natural groundwater conditions. Some bacterial families are capable of oxidizing and/or reducing a solid electrode obtaining an energetic advantage for their own growth. In the present study, we assessed the possibility of stimulating bioelectrochemical reduction of Cr(VI) in a dual-chamber polarized system using an electrode as the sole energy source. To develop an electroactive microbial community three electrodes were, at first, inserted into the anodic compartment of a dual-chamber microbial fuel cell, and inoculated with sludge from an anaerobic digester. After a period of acclimation, one electrode was transferred into a polarized system and it was fixed at −0.3 V (versus standard hydrogen electrode, SHE), to promote the reduction of 1000 µg Cr(VI) L−1. A second electrode, served for the set-up of an open circuit control, operated in parallel. Cr(VI) dissolved concentration was analysed at the initial, during the experiment and final time by spectrophotometric method. Initial and final microbial characterization of the communities enriched in polarized system and open circuit control was performed by 16S rRNA gene sequencing. The bioelectrode set at −0.3 V showed high Cr(VI) removal efficiency (up to 93%) and about 150 µg L−1 day−1 removal rate. Similar efficiency was observed in the open circuit (OC) even at about half rate. Whereas, purely electrochemical reduction, limited to 35%, due to neutral operating conditions. These results suggest that bioelectrochemical Cr(VI) removal by polarized electrode offers a promising new and sustainable approach to the treatment of groundwater Cr(VI) plumes, deserving further research.

ACS Style

Gabriele Beretta; Matteo Daghio; Anna Espinoza Tofalos; Andrea Franzetti; Andrea Filippo Mastorgio; Sabrina Saponaro; Elena Sezenna. Microbial Assisted Hexavalent Chromium Removal in Bioelectrochemical Systems. Water 2020, 12, 466 .

AMA Style

Gabriele Beretta, Matteo Daghio, Anna Espinoza Tofalos, Andrea Franzetti, Andrea Filippo Mastorgio, Sabrina Saponaro, Elena Sezenna. Microbial Assisted Hexavalent Chromium Removal in Bioelectrochemical Systems. Water. 2020; 12 (2):466.

Chicago/Turabian Style

Gabriele Beretta; Matteo Daghio; Anna Espinoza Tofalos; Andrea Franzetti; Andrea Filippo Mastorgio; Sabrina Saponaro; Elena Sezenna. 2020. "Microbial Assisted Hexavalent Chromium Removal in Bioelectrochemical Systems." Water 12, no. 2: 466.

Journal article
Published: 25 January 2020 in Water
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Bioelectrochemical systems (BESs) exploit the interaction between microbes and electrodes. A field of application thereof is bioelectrochemical remediation, an effective strategy in environments where the absence of suitable electron acceptors limits classic bioremediation approaches. Understanding the microbial community structure and genetic potential of anode biofilms is of great interest to interpret the mechanisms occurring in BESs. In this study, by using a whole metagenome sequencing approach, taxonomic and functional diversity patterns in the inoculum and on the anodes of three continuous-flow BES for the removal of phenol, toluene, and BTEX were obtained. The genus Geobacter was highly enriched on the anodes and two reconstructed genomes were taxonomically related to the Geobacteraceae family. To functionally characterize the microbial community, the genes coding for the anaerobic degradation of toluene, ethylbenzene, and phenol were selected as genetic markers for the anaerobic degradation of the pollutants. The genes related with direct extracellular electron transfer (EET) were also analyzed. The inoculum carried the genetic baggage for the degradation of aromatics but lacked the capacity of EET while anodic bacterial communities were able to pursue both processes. The metagenomic approach provided useful insights into the ecology and complex functions within hydrocarbon-degrading electrogenic biofilms.

ACS Style

Anna Espinoza-Tofalos; Matteo Daghio; Enza Palma; Federico Aulenta; Andrea Franzetti. Structure and Functions of Hydrocarbon-Degrading Microbial Communities in Bioelectrochemical Systems. Water 2020, 12, 343 .

AMA Style

Anna Espinoza-Tofalos, Matteo Daghio, Enza Palma, Federico Aulenta, Andrea Franzetti. Structure and Functions of Hydrocarbon-Degrading Microbial Communities in Bioelectrochemical Systems. Water. 2020; 12 (2):343.

Chicago/Turabian Style

Anna Espinoza-Tofalos; Matteo Daghio; Enza Palma; Federico Aulenta; Andrea Franzetti. 2020. "Structure and Functions of Hydrocarbon-Degrading Microbial Communities in Bioelectrochemical Systems." Water 12, no. 2: 343.

Chapter
Published: 18 January 2020 in Applied Environmental Science and Engineering for a Sustainable Future
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Non-halogenated and halogenated hydrocarbons are widespread toxic contaminants. Several physicochemical and biological strategies have been developed for the remediation of contaminated environments. The main goal of biological approaches is to stimulate the microbial activity by overcoming limiting factors such as the presence of nutrients and/or the presence of electron acceptors/donors. Bioelectrochemical systems (BESs) are innovative devices that can be exploited for the bioremediation of polluted environments. In a BES an electrode can be used by several groups of bacteria as solid electron acceptor (anode) or donor (cathode) and the electrons that flow from the anode to the cathode produce an electrical current. BESs have been successfully applied to remove both non-halogenated and halogenated hydrocarbons in lab-scale studies. Several advantages can be obtained using electrodes for the stimulation of the microbial degradation: operational costs can be potentially decreased, the electrical current generated can be used for the real-time monitoring of the degradation, the potential of the electrode (i.e., electron acceptor/donor) can be controlled and adapted to the conditions. This chapter discusses the key aspects linked to the application of bioelectrochemical technologies for the removal of non-halogenated and halogenated hydrocarbons.

ACS Style

Matteo Daghio; Andrea Franzetti. Bioelectrochemical Processes for the Treatment of Oil-Contaminated Water and Sediments. Applied Environmental Science and Engineering for a Sustainable Future 2020, 373 -394.

AMA Style

Matteo Daghio, Andrea Franzetti. Bioelectrochemical Processes for the Treatment of Oil-Contaminated Water and Sediments. Applied Environmental Science and Engineering for a Sustainable Future. 2020; ():373-394.

Chicago/Turabian Style

Matteo Daghio; Andrea Franzetti. 2020. "Bioelectrochemical Processes for the Treatment of Oil-Contaminated Water and Sediments." Applied Environmental Science and Engineering for a Sustainable Future , no. : 373-394.

Review
Published: 07 November 2019 in Water
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Chromium is one of the most frequently used metal contaminants. Its hexavalent form Cr(VI), which is exploited in many industrial activities, is highly toxic, is water-soluble in the full pH range, and is a major threat to groundwater resources. Alongside traditional approaches to Cr(VI) treatment based on physical-chemical methods, technologies exploiting the ability of several microorganisms to reduce toxic and mobile Cr(VI) to the less toxic and stable Cr(III) form have been developed to improve the cost-effectiveness and sustainability of remediating hexavalent chromium-contaminated groundwater. Bioelectrochemical systems (BESs), principally investigated for wastewater treatment, may represent an innovative option for groundwater remediation. By using electrodes as virtually inexhaustible electron donors and acceptors to promote microbial oxidation-reduction reactions, in in situ remediation, BESs may offer the advantage of limited energy and chemicals requirements in comparison to other bioremediation technologies, which rely on external supplies of limiting inorganic nutrients and electron acceptors or donors to ensure proper conditions for microbial activity. Electron transfer is continuously promoted/controlled in terms of current or voltage application between the electrodes, close to which electrochemically active microorganisms are located. Therefore, this enhances the options of process real-time monitoring and control, which are often limited in in situ treatment schemes. This paper reviews research with BESs for treating chromium-contaminated wastewater, by focusing on the perspectives for Cr(VI) bioelectrochemical remediation and open research issues.

ACS Style

Gabriele Beretta; Matteo Daghio; Anna Espinoza Tofalos; Andrea Franzetti; Andrea Filippo Mastorgio; Sabrina Saponaro; Elena Sezenna. Progress Towards Bioelectrochemical Remediation of Hexavalent Chromium. Water 2019, 11, 2336 .

AMA Style

Gabriele Beretta, Matteo Daghio, Anna Espinoza Tofalos, Andrea Franzetti, Andrea Filippo Mastorgio, Sabrina Saponaro, Elena Sezenna. Progress Towards Bioelectrochemical Remediation of Hexavalent Chromium. Water. 2019; 11 (11):2336.

Chicago/Turabian Style

Gabriele Beretta; Matteo Daghio; Anna Espinoza Tofalos; Andrea Franzetti; Andrea Filippo Mastorgio; Sabrina Saponaro; Elena Sezenna. 2019. "Progress Towards Bioelectrochemical Remediation of Hexavalent Chromium." Water 11, no. 11: 2336.

Journal article
Published: 21 October 2019 in Molecules
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Two by-products containing phenols and polysaccharides, a “pâté” (OP) from the extra virgin olive oil milling process and a decoction of pomegranate mesocarp (PM), were investigated for their effects on human microbiota using the SHIME® system. The ability of these products to modulate the microbial community was studied simulating a daily intake for nine days. Microbial functionality, investigated in terms of short chain fatty acids (SCFA) and NH4+, was stable during the treatment. A significant increase in Lactobacillaceae and Bifidobacteriaceae at nine days was induced by OP mainly in the proximal tract. Polyphenol metabolism indicated the formation of tyrosol from OP mainly in the distal tract, while urolithins C and A were produced from PM, identifying the human donor as a metabotype A. The results confirm the SHIME® system as a suitable in vitro tool to preliminarily investigate interactions between complex botanicals and human microbiota before undertaking more challenging human studies.

ACS Style

Camilla Giuliani; Massimo Marzorati; Matteo Daghio; Andrea Franzetti; Marzia Innocenti; Tom Van De Wiele; Nadia Mulinacci; Van De Wiele. Effects of Olive and Pomegranate By-Products on Human Microbiota: A Study Using the SHIME® in Vitro Simulator. Molecules 2019, 24, 3791 .

AMA Style

Camilla Giuliani, Massimo Marzorati, Matteo Daghio, Andrea Franzetti, Marzia Innocenti, Tom Van De Wiele, Nadia Mulinacci, Van De Wiele. Effects of Olive and Pomegranate By-Products on Human Microbiota: A Study Using the SHIME® in Vitro Simulator. Molecules. 2019; 24 (20):3791.

Chicago/Turabian Style

Camilla Giuliani; Massimo Marzorati; Matteo Daghio; Andrea Franzetti; Marzia Innocenti; Tom Van De Wiele; Nadia Mulinacci; Van De Wiele. 2019. "Effects of Olive and Pomegranate By-Products on Human Microbiota: A Study Using the SHIME® in Vitro Simulator." Molecules 24, no. 20: 3791.

Journal article
Published: 05 September 2019 in Animals
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Chestnut tannins (CT) and saturated short medium chain fatty acids (SMCFA) are valid alternatives to contrast the growth of pathogens in poultry rearing, representing a valid alternative to antibiotics. However, the effect of their blends has never been tested. Two blends of CT extract and Sn1-monoglycerides of SMCFA (SN1) were tested in vitro against the proliferation of Clostridium perfringens, Salmonella typhymurium, Escherichia coli, Campylobacter jejuni. The tested concentrations were: 3.0 g/kg of CT; 3.0 g/kg of SN1; 2.0 g/kg of CT and 1.0 g/kg of SN1; 1.0 g/kg of CT and 2.0 g/kg of SN1. Furthermore, their effect on broiler performances and meat quality was evaluated in vivo: one-hundred Ross 308 male birds were fed a basal diet with no supplement (control group) or supplemented with CT or SN1 or their blends at the same concentration used in the in vitro trial. The in vitro assay confirmed the effectiveness of the CT and SN1 mixtures in reducing the growth of the tested bacteria while the in vivo trial showed that broiler performances, animal welfare and meat quality were not negatively affected by the blends, which could be a promising alternative in replacing antibiotics in poultry production.

ACS Style

Federica Mannelli; Sara Minieri; Giovanni Tosi; Giulia Secci; Matteo Daghio; Paola Massi; Laura Fiorentini; Ilaria Galigani; Silvano Lancini; Stefano Rapaccini; Mauro Antongiovanni; Simone Mancini; Arianna Buccioni. Effect of Chestnut Tannins and Short Chain Fatty Acids as Anti-Microbials and as Feeding Supplements in Broilers Rearing and Meat Quality. Animals 2019, 9, 659 .

AMA Style

Federica Mannelli, Sara Minieri, Giovanni Tosi, Giulia Secci, Matteo Daghio, Paola Massi, Laura Fiorentini, Ilaria Galigani, Silvano Lancini, Stefano Rapaccini, Mauro Antongiovanni, Simone Mancini, Arianna Buccioni. Effect of Chestnut Tannins and Short Chain Fatty Acids as Anti-Microbials and as Feeding Supplements in Broilers Rearing and Meat Quality. Animals. 2019; 9 (9):659.

Chicago/Turabian Style

Federica Mannelli; Sara Minieri; Giovanni Tosi; Giulia Secci; Matteo Daghio; Paola Massi; Laura Fiorentini; Ilaria Galigani; Silvano Lancini; Stefano Rapaccini; Mauro Antongiovanni; Simone Mancini; Arianna Buccioni. 2019. "Effect of Chestnut Tannins and Short Chain Fatty Acids as Anti-Microbials and as Feeding Supplements in Broilers Rearing and Meat Quality." Animals 9, no. 9: 659.

Journal article
Published: 18 July 2019 in Microorganisms
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The addition of polyphenol extracts in ruminant diets is an effective strategy to modulate rumen microflora. The aim of this in vitro trial was to study the effects of chestnut tannin extract (CHT), vescalagin (VES) and gallic acid (GAL) on dietary fibre degradability and on the dimethyl acetals (DMA) profile and microbial community composition of rumen liquor. Four diets (basal diet; basal diet plus CHT; basal diet plus VES; basal diet plus GAL) were fermented for 24 h using ewe rumen liquor. At the end of the fermentation, the microbial communities were characterized by sequencing the 16S rRNA gene. The DMA profile was analyzed by gas chromatography. Chestnut tannin extract did not affect fibre degradability, whereas VES and GAL showed a detrimental effect. The presence of CHT, VES and GAL influenced the concentration of several DMA (i.e., 12:0, 13:0, 14:0, 15:0, 18:0 and 18:1 trans-11), whereas the composition of the microbial community was marginally affected. The inclusion of CHT led to the enrichment of the genera Anaerovibrio, Bibersteinia, Escherichia/Shigella, Pseudobutyrivibrio and Streptococcus. The results of this study support the hypothesis that the activity of CHT is due to the synergistic effect of all components rather than the property of a single component.

ACS Style

Federica Mannelli; Matteo Daghio; Susana Alves; Rui J. B. Bessa; Sara Minieri; Luciana Giovannetti; Giuseppe Conte; Marcello Mele; Anna Messini; Stefano Rapaccini; Carlo Viti; Arianna Buccioni. Effects of Chestnut Tannin Extract, Vescalagin and Gallic Acid on the Dimethyl Acetals Profile and Microbial Community Composition in Rumen Liquor: An In Vitro Study. Microorganisms 2019, 7, 202 .

AMA Style

Federica Mannelli, Matteo Daghio, Susana Alves, Rui J. B. Bessa, Sara Minieri, Luciana Giovannetti, Giuseppe Conte, Marcello Mele, Anna Messini, Stefano Rapaccini, Carlo Viti, Arianna Buccioni. Effects of Chestnut Tannin Extract, Vescalagin and Gallic Acid on the Dimethyl Acetals Profile and Microbial Community Composition in Rumen Liquor: An In Vitro Study. Microorganisms. 2019; 7 (7):202.

Chicago/Turabian Style

Federica Mannelli; Matteo Daghio; Susana Alves; Rui J. B. Bessa; Sara Minieri; Luciana Giovannetti; Giuseppe Conte; Marcello Mele; Anna Messini; Stefano Rapaccini; Carlo Viti; Arianna Buccioni. 2019. "Effects of Chestnut Tannin Extract, Vescalagin and Gallic Acid on the Dimethyl Acetals Profile and Microbial Community Composition in Rumen Liquor: An In Vitro Study." Microorganisms 7, no. 7: 202.

Journal article
Published: 27 June 2019 in New Biotechnology
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Microbial electrochemical technologies (MET) are increasingly being considered for in situ remediation of contaminated groundwater. However, their application potential for the simultaneous treatment of complex mixtures of organic and inorganic contaminants, has been only marginally explored. Here we have analyzed the performance of the ‘bioelectric well’, a previously developed bioelectrochemical reactor configuration, in the treatment of benzene, toluene, ethyl-benzene and xylenes (BTEX) mixtures. Although to different extents, all BTEX were found to be degraded in the bioelectrochemical system, operated using a continuous-flow of groundwater at a hydraulic retention time of 8.8 h, with the graphite anode potentiostatically controlled at +0.200 V vs. the standard hydrogen electrode. In the case of toluene and ethyl-benzene, biodegradation was further confirmed by the GC-MS identification of fumarate-addition metabolites, previously shown to be involved in the activation of these contaminants under anaerobic conditions. Degradation rates were higher for toluene (31.3 ± 1.5 mg/L d) and lower for benzene (6.1 ± 0.3 mg/L d), ethyl-benzene (3.3 ± 0.1 mg/L d), and xylenes (4.5 ± 0.2 mg/L d). BTEX degradation was linked to electric current generation, with coulombic efficiencies falling in the range 53-69%, although methanogenesis also contributed to contaminant degradation. Remarkably, the system also allowed removal of sulfate simultaneously with toluene. Sulfate removal likely exploited the hydrogen abiotically generated at the cathode to fuel the metabolism of sulfate-reducing bacteria. Taken as a whole, these findings highlight the remarkable potential of this innovative reactor configuration for application in a variety of contamination scenarios.

ACS Style

Enza Palma; Anna Espinoza Tofalos; Matteo Daghio; Andrea Franzetti; Panagiota Tsiota; Carolina Cruz Viggi; Marco Petrangeli Papini; Federico Aulenta. Bioelectrochemical treatment of groundwater containing BTEX in a continuous-flow system: Substrate interactions, microbial community analysis, and impact of sulfate as a co-contaminant. New Biotechnology 2019, 53, 41 -48.

AMA Style

Enza Palma, Anna Espinoza Tofalos, Matteo Daghio, Andrea Franzetti, Panagiota Tsiota, Carolina Cruz Viggi, Marco Petrangeli Papini, Federico Aulenta. Bioelectrochemical treatment of groundwater containing BTEX in a continuous-flow system: Substrate interactions, microbial community analysis, and impact of sulfate as a co-contaminant. New Biotechnology. 2019; 53 ():41-48.

Chicago/Turabian Style

Enza Palma; Anna Espinoza Tofalos; Matteo Daghio; Andrea Franzetti; Panagiota Tsiota; Carolina Cruz Viggi; Marco Petrangeli Papini; Federico Aulenta. 2019. "Bioelectrochemical treatment of groundwater containing BTEX in a continuous-flow system: Substrate interactions, microbial community analysis, and impact of sulfate as a co-contaminant." New Biotechnology 53, no. : 41-48.

Review
Published: 01 May 2019 in Journal of Dairy Science
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The interest of the scientific community in the effects of plant polyphenols on animal nutrition is increasing. These compounds, in fact, are ubiquitous in the plant kingdom, especially in some spontaneous plants exploited as feeding resources alternative to cultivated crops and in several agro-industry by-products. Polyphenols interact with rumen microbiota, affecting carbohydrate fermentation, protein degradation, and lipid metabolism. Some of these aspects have been largely reviewed, especially for tannins; however, less information is available about the direct effect of polyphenols on the composition of rumen microbiota. In the present paper, we review the most recent literature about the effect of plant polyphenols on rumen microbiota responsible for unsaturated fatty acid biohydrogenation, fiber digestion, and methane production, taking into consideration the advances in microbiota analysis achieved in the last 10 yr. Key aspects, such as sample collection, sample storage, DNA extraction, and the main phylogenetic markers used in the reconstruction of microbial community structure, are examined. Furthermore, a summary of the new high-throughput methods based on next generation sequencing is reviewed. Several effects can be associated with dietary polyphenols. Polyphenols are able to depress or modulate the biohydrogenation of unsaturated fatty acids by a perturbation of ruminal microbiota composition. In particular, condensed tannins have an inhibitory effect on biohydrogenation, whereas hydrolyzable tannins seem to have a modulatory effect on biohydrogenation. With regard to fiber digestion, data from literature are quite consistent about a general depressive effect of polyphenols on gram-positive fibrolytic bacteria and ciliate protozoa, resulting in a reduction of volatile fatty acid production (mostly acetate molar production). Methane production is also usually reduced when tannins are included in the diet of ruminants, probably as a consequence of the inhibition of fiber digestion. However, some evidence suggests that hydrolyzable tannins may reduce methane emission by directly interacting with rumen microbiota without affecting fiber digestion.

ACS Style

V. Vasta; Matteo Daghio; A. Cappucci; A. Buccioni; A. Serra; C. Viti; M. Mele. Invited review: Plant polyphenols and rumen microbiota responsible for fatty acid biohydrogenation, fiber digestion, and methane emission: Experimental evidence and methodological approaches. Journal of Dairy Science 2019, 102, 3781 -3804.

AMA Style

V. Vasta, Matteo Daghio, A. Cappucci, A. Buccioni, A. Serra, C. Viti, M. Mele. Invited review: Plant polyphenols and rumen microbiota responsible for fatty acid biohydrogenation, fiber digestion, and methane emission: Experimental evidence and methodological approaches. Journal of Dairy Science. 2019; 102 (5):3781-3804.

Chicago/Turabian Style

V. Vasta; Matteo Daghio; A. Cappucci; A. Buccioni; A. Serra; C. Viti; M. Mele. 2019. "Invited review: Plant polyphenols and rumen microbiota responsible for fatty acid biohydrogenation, fiber digestion, and methane emission: Experimental evidence and methodological approaches." Journal of Dairy Science 102, no. 5: 3781-3804.

Journals
Published: 30 October 2018 in Environmental Science: Water Research & Technology
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High-rate anaerobic oxidation of toluene was achieved in a continuous-flow bioelectrochemical system.

ACS Style

Enza Palma; Matteo Daghio; Anna Espinoza Tofalos; Andrea Franzetti; Carolina Cruz Viggi; Stefano Fazi; Marco Petrangeli Papini; Federico Aulenta. Anaerobic electrogenic oxidation of toluene in a continuous-flow bioelectrochemical reactor: process performance, microbial community analysis, and biodegradation pathways. Environmental Science: Water Research & Technology 2018, 4, 2136 -2145.

AMA Style

Enza Palma, Matteo Daghio, Anna Espinoza Tofalos, Andrea Franzetti, Carolina Cruz Viggi, Stefano Fazi, Marco Petrangeli Papini, Federico Aulenta. Anaerobic electrogenic oxidation of toluene in a continuous-flow bioelectrochemical reactor: process performance, microbial community analysis, and biodegradation pathways. Environmental Science: Water Research & Technology. 2018; 4 (12):2136-2145.

Chicago/Turabian Style

Enza Palma; Matteo Daghio; Anna Espinoza Tofalos; Andrea Franzetti; Carolina Cruz Viggi; Stefano Fazi; Marco Petrangeli Papini; Federico Aulenta. 2018. "Anaerobic electrogenic oxidation of toluene in a continuous-flow bioelectrochemical reactor: process performance, microbial community analysis, and biodegradation pathways." Environmental Science: Water Research & Technology 4, no. 12: 2136-2145.

Journal article
Published: 16 August 2018 in Journal of Hazardous Materials
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Sulfate reducing microorganisms are typically involved in hydrocarbon biodegradation in the sea sediment, with their metabolism resulting in the by-production of toxic sulfide. In this context, it is of utmost importance identifying the optimal value for anodic potential which ensures efficient toxic sulfide removal. Along this line, in this study the (bio)electrochemical removal of sulfide was tested at anodic potentials of −205 mV, +195 mV and +300 mV vs Ag/AgCl), also in the presence of a pure culture of the sulfur-oxidizing bacterium Desulfobulbus propionicus. Current production, sulfide concentration and sulfate concentration were monitored over time. At the end of the experiment sulfur deposition on the electrodes and the microbial communities were characterized by SEM-EDS and by next generation sequencing of the 16S rRNA gene respectively. Results confirmed that current production is linked to sulfide removal and D. propionicus promoted back oxidation of deposited sulfur to sulfate. The highest electron recovery was observed at +195 mV vs Ag/AgCl, and the lowest sulfur deposition was obtained at −205 mV vs Ag/AgCl anode polarization.

ACS Style

Matteo Daghio; Eleni Vaiopoulou; Federico Aulenta; Angela Sherry; Ian Head; Andrea Franzetti; Korneel Rabaey. Anode potential selection for sulfide removal in contaminated marine sediments. Journal of Hazardous Materials 2018, 360, 498 -503.

AMA Style

Matteo Daghio, Eleni Vaiopoulou, Federico Aulenta, Angela Sherry, Ian Head, Andrea Franzetti, Korneel Rabaey. Anode potential selection for sulfide removal in contaminated marine sediments. Journal of Hazardous Materials. 2018; 360 ():498-503.

Chicago/Turabian Style

Matteo Daghio; Eleni Vaiopoulou; Federico Aulenta; Angela Sherry; Ian Head; Andrea Franzetti; Korneel Rabaey. 2018. "Anode potential selection for sulfide removal in contaminated marine sediments." Journal of Hazardous Materials 360, no. : 498-503.

Journal article
Published: 16 May 2018 in FEMS Microbiology Letters
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Bioelectrochemical remediation of hydrocarbons is a technology that exploits the ability of specific microorganisms to use as electron acceptor an electrode, thus potentially lowering the operational costs related to classical bioremediation. Several well-characterized hydrocarbonoclastic strains might be electroactive, thus their biodegradation performances in Bioelectrochemical Systems should be studied. Cupriavidus metallidurans CH34 is a model metal-resistant strain whose capacity to degrade benzene aerobically has recently been described. In this study, toluene degradation under anaerobic conditions and the exoelectrogenic capacity of Cupriavidus metallidurans CH34 were determined. Strain CH34 was grown anaerobically with toluene as sole carbon source in sealed serum bottles and then inoculated in a Microbial Electrolysis Cell (MEC) to assess its exoelectrogenic capacity. It was demonstrated for the first time that strain CH34 is able to degrade toluene under nitrate-reducing conditions (up to 45 mgtoluene/L were removed within 17 days, corresponding to 73% of toluene amended). Nitrate consumption and cellular growth were observed during toluene removal. In the MEC, toluene degradation was linked to current production, showing current peaks after every toluene addition (maximum current density 48 mA/m2). Coulombic efficiency of the toluene biodegradation process increased with time, from 11% (first batch cycle), up to 77% (last batch cycle).

ACS Style

Anna Espinoza Tofalos; Matteo Daghio; Myriam González; Maddalena Papacchini; Andrea Franzetti; Michael Seeger. Toluene degradation by Cupriavidus metallidurans CH34 in nitrate-reducing conditions and in Bioelectrochemical Systems. FEMS Microbiology Letters 2018, 1 .

AMA Style

Anna Espinoza Tofalos, Matteo Daghio, Myriam González, Maddalena Papacchini, Andrea Franzetti, Michael Seeger. Toluene degradation by Cupriavidus metallidurans CH34 in nitrate-reducing conditions and in Bioelectrochemical Systems. FEMS Microbiology Letters. 2018; ():1.

Chicago/Turabian Style

Anna Espinoza Tofalos; Matteo Daghio; Myriam González; Maddalena Papacchini; Andrea Franzetti; Michael Seeger. 2018. "Toluene degradation by Cupriavidus metallidurans CH34 in nitrate-reducing conditions and in Bioelectrochemical Systems." FEMS Microbiology Letters , no. : 1.

Journal article
Published: 25 July 2017 in Journal of Hazardous Materials
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BTEX compounds (Benzene, Toluene, Ethylbenzene and Xylenes) are toxic hydrocarbons that can be found in groundwater due to accidental spills. Bioelectrochemical systems (BES) are an innovative technology to stimulate the anaerobic degradation of hydrocarbons. In this work, single chamber BESs were used to assess the degradation of a BTEX mixture at different applied voltages (0.8 V, 1.0 V, 1.2 V) between the electrodes. Hydrocarbon degradation was linked to current production and to sulfate reduction, at all the tested potentials. The highest current densities (about 200 mA/m2 with a maximum peak at 480 mA/m2) were observed when 0.8 V were applied. The application of an external voltage increased the removal of toluene, m-xylene and p-xylene. The highest removal rate constants at 0.8 V were: 0.4 ± 0.1 days−1, 0.34 ± 0.09 days−1 and 0.16 ± 0.02 days−1, respectively. At the end of the experiment, the microbial communities were characterized by high throughput sequencing of the 16S rRNA gene. Microorganisms belonging to the families Desulfobulbaceae, Desulfuromonadaceae and Geobacteraceae were enriched on the anodes suggesting that both direct electron transfer and sulfur cycling occurred. The cathodic communities were dominated by the family Desulfomicrobiaceae that may be involved in hydrogen production.

ACS Style

Matteo Daghio; Anna Espinoza Tofalos; Barbara Leoni; Pierangela Cristiani; Maddalena Papacchini; Elham Jalilnejad; Giuseppina Bestetti; Andrea Franzetti. Bioelectrochemical BTEX removal at different voltages: assessment of the degradation and characterization of the microbial communities. Journal of Hazardous Materials 2017, 341, 120 -127.

AMA Style

Matteo Daghio, Anna Espinoza Tofalos, Barbara Leoni, Pierangela Cristiani, Maddalena Papacchini, Elham Jalilnejad, Giuseppina Bestetti, Andrea Franzetti. Bioelectrochemical BTEX removal at different voltages: assessment of the degradation and characterization of the microbial communities. Journal of Hazardous Materials. 2017; 341 ():120-127.

Chicago/Turabian Style

Matteo Daghio; Anna Espinoza Tofalos; Barbara Leoni; Pierangela Cristiani; Maddalena Papacchini; Elham Jalilnejad; Giuseppina Bestetti; Andrea Franzetti. 2017. "Bioelectrochemical BTEX removal at different voltages: assessment of the degradation and characterization of the microbial communities." Journal of Hazardous Materials 341, no. : 120-127.

Journal article
Published: 11 July 2017 in Microbial Biotechnology
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Groundwater contamination by petroleum hydrocarbons (PHs) is a widespread problem which poses serious environmental and health concerns. Recently, microbial electrochemical technologies (MET) have attracted considerable attention for remediation applications, having the potential to overcome some of the limiting factors of conventional in situ bioremediation systems. So far, field-scale application of MET has been largely hindered by the limited availability of scalable system configurations. Here, we describe the ‘bioelectric well’ a bioelectrochemical reactor configuration, which can be installed directly within groundwater wells and can be applied for in situ treatment of organic contaminants, such as PHs. A laboratory-scale prototype of the bioelectric well has been set up and operated in continuous-flow regime with phenol as the model contaminant. The best performance was obtained when the system was inoculated with refinery sludge and the anode potentiostatically controlled at +0.2 V versus SHE. Under this condition, the influent phenol (25 mg l−1) was nearly completely (99.5 ± 0.4%) removed, with an average degradation rate of 59 ± 3 mg l−1 d and a coulombic efficiency of 104 ± 4%. Microbial community analysis revealed a remarkable enrichment of Geobacter species on the surface of the graphite anode, clearly pointing to a direct involvement of this electro-active bacterium in the current-generating and phenol-oxidizing process.

ACS Style

Enza Palma; Matteo Daghio; Andrea Franzetti; Marco Petrangeli Papini; Federico Aulenta. The bioelectric well: a novel approach forin situtreatment of hydrocarbon-contaminated groundwater. Microbial Biotechnology 2017, 11, 112 -118.

AMA Style

Enza Palma, Matteo Daghio, Andrea Franzetti, Marco Petrangeli Papini, Federico Aulenta. The bioelectric well: a novel approach forin situtreatment of hydrocarbon-contaminated groundwater. Microbial Biotechnology. 2017; 11 (1):112-118.

Chicago/Turabian Style

Enza Palma; Matteo Daghio; Andrea Franzetti; Marco Petrangeli Papini; Federico Aulenta. 2017. "The bioelectric well: a novel approach forin situtreatment of hydrocarbon-contaminated groundwater." Microbial Biotechnology 11, no. 1: 112-118.

Review
Published: 01 May 2017 in Water Research
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Annually, thousands of oil spills occur across the globe. As a result, petroleum substances and petrochemical compounds are widespread contaminants causing concern due to their toxicity and recalcitrance. Many remediation strategies have been developed using both physicochemical and biological approaches. Biological strategies are most benign, aiming to enhance microbial metabolic activities by supplying limiting inorganic nutrients, electron acceptors or donors, thus stimulating oxidation or reduction of contaminants. A key issue is controlling the supply of electron donors/acceptors. Bioelectrochemical systems (BES) have emerged, in which an electrical current serves as either electron donor or acceptor for oil spill bioremediation. BES are highly controllable and can possibly also serve as biosensors for real time monitoring of the degradation process. Despite being promising, multiple aspects need to be considered to make BES suitable for field applications including system design, electrode materials, operational parameters, mode of action and radius of influence. The microbiological processes, involved in bioelectrochemical contaminant degradation, are currently not fully understood, particularly in relation to electron transfer mechanisms. Especially in sulfate rich environments, the sulfur cycle appears pivotal during hydrocarbon oxidation. This review provides a comprehensive analysis of the research on bioelectrochemical remediation of oil spills and of the key parameters involved in the process.

ACS Style

Matteo Daghio; Federico Aulenta; Eleni Vaiopoulou; Andrea Franzetti; Jan B.A. Arends; Angela Sherry; Ana Suárez-Suárez; Ian Head; Giuseppina Bestetti; Korneel Rabaey. Electrobioremediation of oil spills. Water Research 2017, 114, 351 -370.

AMA Style

Matteo Daghio, Federico Aulenta, Eleni Vaiopoulou, Andrea Franzetti, Jan B.A. Arends, Angela Sherry, Ana Suárez-Suárez, Ian Head, Giuseppina Bestetti, Korneel Rabaey. Electrobioremediation of oil spills. Water Research. 2017; 114 ():351-370.

Chicago/Turabian Style

Matteo Daghio; Federico Aulenta; Eleni Vaiopoulou; Andrea Franzetti; Jan B.A. Arends; Angela Sherry; Ana Suárez-Suárez; Ian Head; Giuseppina Bestetti; Korneel Rabaey. 2017. "Electrobioremediation of oil spills." Water Research 114, no. : 351-370.

Journal article
Published: 06 December 2016 in Journal of The Electrochemical Society
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Microbial fuel cells (MFCs) are attracting considerable attention as innovative systems for energy production from renewable residual biomass and biomass-derived wastes dissolved in wastewaters. The current produced by a microbial fuel cell can also be used to quantify the rate of specific metabolic processes and the substrate concentration in real time. Aim of this work is the study of the correlation between the decay of current density in a microbial fuel cell and the concentration of the residual organic substrates when it reaches low concentration, in the rage of 0–500 mg/L COD. Tests were performed in continuous flow using an air breathing, membraneless MFC using sodium acetate as organic substrate. A direct concentration-dependent current output was achieved in the range of 0–100 mg/l, with a Monod kinetics as the best-fitting model. A step of current was also achieved at concentration higher than 120 mg/L.

ACS Style

Andrea Franzetti; M. Daghio; P. Parenti; T. Truppi; G. Bestetti; S. P. Trasatti; P. Cristiani. Monod Kinetics Degradation of Low Concentration Residual Organics in Membraneless Microbial Fuel Cells. Journal of The Electrochemical Society 2016, 164, H3091 -H3096.

AMA Style

Andrea Franzetti, M. Daghio, P. Parenti, T. Truppi, G. Bestetti, S. P. Trasatti, P. Cristiani. Monod Kinetics Degradation of Low Concentration Residual Organics in Membraneless Microbial Fuel Cells. Journal of The Electrochemical Society. 2016; 164 (3):H3091-H3096.

Chicago/Turabian Style

Andrea Franzetti; M. Daghio; P. Parenti; T. Truppi; G. Bestetti; S. P. Trasatti; P. Cristiani. 2016. "Monod Kinetics Degradation of Low Concentration Residual Organics in Membraneless Microbial Fuel Cells." Journal of The Electrochemical Society 164, no. 3: H3091-H3096.

Review article
Published: 21 November 2016 in Frontiers in Microbiology
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Widespread pollution of terrestrial ecosystems with petroleum hydrocarbons (PHCs) has generated a need for remediation and, given that many PHCs are biodegradable, bio- and phyto-remediation are often viable approaches for active and passive remediation. This review focuses on phytoremediation with particular interest on the interactions between and use of plant – associated bacteria to restore PHC polluted sites. Plant-associated bacteria include endophytic, phyllospheric and rhizospheric bacteria, and cooperation between these bacteria and their host plants allows for greater plant survivability and treatment outcomes in contaminated sites. Bacterially-driven PHC bioremediation is attributed to the presence of diverse suites of metabolic genes for aliphatic and aromatic hydrocarbons, along with a broader suite of physiological properties including biosurfactant production, biofilm formation, chemotaxis to hydrocarbons, and flexibility in cell-surface hydrophobicity. In soils impacted by PHC contamination, microbial bioremediation generally relies on the addition of high-energy electron acceptors (e.g. oxygen) and fertilization to supply limiting nutrients (e.g. nitrogen, phosphorous, potassium) in the face of excess PHC carbon. As an alternative, the addition of plants can greatly improve bioremediation rates and outcomes as plants provide microbial habitats, improve soil porosity (thereby increasing mass transfer of substrates and electron acceptors), and exchange limiting nutrients with their microbial counterparts. In return, plant-associated microorganisms improve plant growth by reducing soil toxicity through contaminant removal, producing plant growth promoting metabolites, liberating sequestered plant nutrients from soil, fixing nitrogen, and more generally establishing the foundations of soil nutrient cycling. In a practical and applied sense, the collective action of plants and their associated microorganisms is advantageous for remediation of PHC contaminated soil in terms of overall cost and success rates for in situ implementation in a diversity of environments. Mechanistically, there remain biological unknowns that present challenges for applying bio- and phyto-remediation technologies without having a deep prior understanding of individual target sites. In this review, evidence from traditional and modern omics technologies is discussed to provide a framework for plant-microbe interactions during PHCs remediation. The potential for integrating multiple molecular and computational techniques to evaluate linkages between microbial communities, plant communities and ecosystem processes is explored with an eye on

ACS Style

Panagiotis Gkorezis; Matteo Daghio; Andrea Franzetti; Jonathan Van Hamme; Wouter Sillen; Jaco Vangronsveld. The Interaction between Plants and Bacteria in the Remediation of Petroleum Hydrocarbons: An Environmental Perspective. Frontiers in Microbiology 2016, 7, 1836 .

AMA Style

Panagiotis Gkorezis, Matteo Daghio, Andrea Franzetti, Jonathan Van Hamme, Wouter Sillen, Jaco Vangronsveld. The Interaction between Plants and Bacteria in the Remediation of Petroleum Hydrocarbons: An Environmental Perspective. Frontiers in Microbiology. 2016; 7 ():1836.

Chicago/Turabian Style

Panagiotis Gkorezis; Matteo Daghio; Andrea Franzetti; Jonathan Van Hamme; Wouter Sillen; Jaco Vangronsveld. 2016. "The Interaction between Plants and Bacteria in the Remediation of Petroleum Hydrocarbons: An Environmental Perspective." Frontiers in Microbiology 7, no. : 1836.