Calcium (Ca) is often supplied to crop species to prevent the occurrence of Ca–related disorders. Mechanisms of Ca absorption and transport are not fully understood and the effectiveness of root and/or foliar Ca fertilization may be variable. To characterize the rate of Ca absorption and transport, trials were developed with chili pepper and sweet cherry plants, using 45CaCl2 as a tracer. The Ca treatments supplied were: (1) No 45Ca (control); (2) 45Ca soil application; (3) 45Ca supply to basal leaves, and (4) 45Ca application to apical leaves. After two months, plants were harvested for biomass and Ca content determination. The recovery of 45Ca in different plant parts was measured with a liquid scintillation counter and leaf traits were observed by scanning electronic microscopy. In general, the highest 45Ca concentrations were recovered in treated organs, while root applications led to highest 45Ca translocation rates, which varied between chili pepper and cherry plants. For chili pepper, 45Ca applied to the soil was detected mainly in roots (44 %) followed by leaves (36.6 %) stems (17.4 %) and fruits (2 %). In sweet cherry trees, soil–applied 45Ca was principally recovered in roots (45.3 %), shoots (28.5 %), leaves (14.3 %) and trunks (11.9 %). The results provide evidence of increased absorption of root–applied Ca, as well as different degrees of Ca mobility between species. Foliar application led to major Ca increases in treated leaves, with Ca transported to other plant organs after apical leaf Ca supply chiefly in cherry trees.

In this study, the rate of absorption and transport of calcium (Ca) in orange fruits (Citrus sinensis cv. Fukumoto) after surface treatment with ⁴⁵Ca was evaluated by supplying treatments at different dates between fruit set and mid-phase II of growth (i.e., 30, 44, 66 and 99 days after full bloom, DAFB). ⁴⁵Ca absorption was always detected, but ⁴⁵Ca application at the fruit set was associated with the highest rates of Ca transport into the pulp (39%) compared to the other treatments (17–19%). Scanning electron microscopy SEM) images of the fruit surface showed the occurrence of stomata along with the entire rind at all the developmental stages evaluated. However, from the beginning of stage I, stomata began to collapse and develop plugs, and this became frequent as the fruit age increased. From 44 DAFB, oil gland density increased, and Ca oxalates (CaOx) were found in the fruit flavedo and albedo. Fruit Ca (not labeled) concentration increased from fruit set (30 DAFB) to 99 DAFB, although oil gland formation and rind growth and differentiation likely hindered Ca transport to internal fruit tissues (pulp). The total Ca concentration in the pedicel was always higher than that in the fruit, with no differences between the first three treatments. The information obtained in this study may be essential for improving Ca fertilizer efficacy in citrus by spray applications.

Nitrogen (N) in a maize crop is a determining yield factor, but its negative impact on the environment is also known. Therefore, it is necessary to propose mitigation strategies that allow an improvement in the N fertilizer efficiency (NFE), such as the use of cover crops (CC) and the adjustment of the fertilizer dose. The objective of the study was to determine NFE using ¹⁵N isotopic techniques and nitrate (NO₃⁻) leaching in a maize–fallow versus a maize–CC rotation with optimal and excessive doses of N in the Mediterranean area of Chile. The treatments were a combination of crop rotation (maize–fallow versus maize–CC of Lolium multiflorum) with the optimal dose of N (250 kg ha⁻¹) or excessive dose (400 kg ha⁻¹). We found that the optimal dose of maize–CC rotation contributed to reducing the losses of N by leaching and improving the NFE. Using the optimal dose decreased the dissolved inorganic N (DIN) emission intensity by 50% compared to the excessive doses. Even if grain yield was higher (19 t ha⁻¹) when applying the excessive N dose, the NFE (28%) was lower than when applying the optimal dose (40%). In the maize–CC rotation with optimal dose, yield was 17 Mg ha⁻¹. The excessive N dose generated higher DIN content at the end of the maize season (177 kg N ha⁻¹). In conclusion, replacing the traditional autumn–winter fallow in the maize monoculture with a CC with optimal N dose contributed to improving NFE and reducing N leaching in a Mediterranean agricultural system. Consequently, it is a strategy to consider as it has positive advantages in soil and N management, helping to reduce diffuse pollution of surface and groundwater bodies.

The use of grafting techniques for horticultural crops increases plant tolerance to various abiotic and biotic stresses. Tomato production under greenhouse conditions relies on plants grafted onto vigorous rootstocks because they sustain crops for longer periods. Growers under Mediterranean conditions usually grow crops in passive greenhouses during the summer and winter season, to provide fresh products throughout the year. No information is available with regard to the effect of the environment on nitrogen-use efficiency (NUE) in tomato plants grafted onto rootstocks with different vigor. In the present study, NUE, along with its components—uptake (NUpE) and utilization (NUtE) efficiencies—were evaluated in tomato plants grafted onto two interspecific rootstocks, conferring medium (“King Kong”) or high (“Kaiser”) vigor to the plants. The evaluations were carried out during the vegetative and reproductive stage in plants subjected to different environmental conditions resulting in different plant growth rates. The grafting treatments did not affect NUE, NUpE or NUtE in young plants, but at the reproductive stage, differences were found during the summer season (high N demand) where the vigorous rootstock increased NUpE from 55%, in non-grafted plants, to 94%, with the consequent differences in NUE. During the winter crop, no differences in NUE were found between the vigorous rootstock and non-grafted plants, but the less vigorous (cold-tolerant) rootstock enhanced NUpE. Significant positive relationships were found between plant growth rate and both NUE and NUpE, while NUtE decreased with increasing growth rate.

The Apalta catchment is a wine-producing area of 1300 ha, lying south of Santiago, located in the Colchagua Valley, near Santa Cruz in the VI Region of Chile. The vineyards are planted on slopes of up to 22°, the rows being aligned mainly down the slope. This cropping system and its management lead to a potential contamination of natural resources. To assess the risk of environmental contamination due to the application of pesticides in different pest control strategies, indicators at a field level were derived according to a step-by-step procedure. Using these indicators, runoff was found to be the main process of impact. The different pest control strategies in this catchment caused different potential risks, though these in general were low.