Utsala Shrestha

Dr. Utsala Shrestha

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Research Focus & Metrics

Academic Career Length

14Years (2013-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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26 August 2016 in Frontiers in Plant Science
Verified
Journal Article
Open access
USUtsala Shrestha
Utsala Shrestha
RARobert M. Augé
Robert M. Augé
DBDavid M Butler
David M Butler
Anaerobic soil disinfestation (ASD) is a proven but relatively new strategy to control soil borne pests of horticultural crops through anaerobic decomposition of organic soil amendments. The ASD technique has primarily been used to control soil borne pathogens; however, this technique has also shown potential to control plant parasitic nematodes and weeds. ASD can utilize a broad range of carbon (C) amendments and optimization may improve efficacy across environments. In this context, a meta-analysis using a random-effects model was conducted to determine effect sizes of the ASD effect on soil borne pathogens (533 studies), plant parasitic nematodes (91 studies), and weeds (88 studies) compared with unamended controls. Yield response to ASD was evaluated (123 studies) compared to unamended and fumigated controls. We also examined moderator variables for environmental conditions and amendments to explore the impact of these moderators on ASD effectiveness on pests and yield. Across all pathogen types with the exception of Sclerotinia spp., ASD studies show suppression of bacterial, oomycete and fungal pathogens (59% to 86%). Pathogen suppression was effective under all environmental conditions (50% to 94%) and amendment types (53% to 98%), except when amendments applied at rates less than 0.3 kg m-2. The ASD effect ranged from 15% to 56% for nematode suppression and 32% to 81% for weed suppression, but these differences were not significant. Significant nematode moderators included study type, soil type, sampling depth, incubation period, and use of mixed amendments. Weed suppression due to ASD showed significant heterogeneity for all environmental conditions, confirming that these studies do not share a common effect size. Total crop yield was not reduced by ASD when compared to a fumigant control and yield was significantly higher (30%) compared to an unamended control, suggesting ASD as a feasible option to maintain yield without chemical soil fumigants. We conclude ASD is effective against soil borne pathogens and while not conclusive due to a limited number of studies, we expect the same for nematodes and weeds given observed effect sizes. Findings should assist researchers in exploring ASD efficacy in particular environmental conditions and allow for development of standard treatment protocols.

1 January 2015
Verified
Book Chapter
Closed access
PDF file
ERErin Rosskopf
Erin Rosskopf
Paula Serrano-PérezPaula Serrano-Pérez
Paula Serrano-Pérez
JHJason Hong
Jason Hong
USUtsala Shrestha
Utsala Shrestha
MRMaría del Carmen Rodríguez-Molina
María del Carmen Rodríguez-Molina
DBDavid M Butler
David M Butler

1 March 2018 in Phytopathology®
Verified
Journal Article
Open access
USUtsala Shrestha
Utsala Shrestha
MDMary E. Dee
Mary E. Dee
BOBonnie H. Ownley
Bonnie H. Ownley
DBDavid M Butler
David M Butler
Growth chamber and field studies were conducted with organic amendment mixtures of carbon (C) and nitrogen (N) at C:N ratios 10:1, 20:1, 30:1, and 40:1 and amendment rates of C at 2, 4, 6, and 8 mg/g of soil (C:N ratio 30:1) to evaluate anaerobic soil disinfestation (ASD) effects on germination and colonization of Sclerotium rolfsii. In the growth chamber, sclerotial germination was reduced in all ASD treatments regardless of C:N ratio (0.6 to 8.5% germination) or amendment rate (7.5 to 46%) as compared with nonamended controls (21 to 36% and 61 to 96%, respectively). ASD treatment increased Trichoderma spp. colonization of sclerotia, with consistently higher colonization in ASD treatments with amendment rates of C at 2 or 4 mg/g of soil (>87% colonization) compared with nonamended controls (<50% colonization). In the 2014 field study, sclerotial germination was reduced by 24 to 30% in ASD treatments when compared with the nonamended control. Sclerotial colonization by Trichoderma spp. was predominant; however, other potential mycoparasites (i.e., Aspergillus spp., Fusarium spp., zygomycetes, and other fungi) were present in the field study. Amendment C:N ratios in the range of 10:1 to 40:1 were equally effective in reducing sclerotial germination and enhancing colonization by potentially beneficial mycoparasites of sclerotia.

1 August 2021 in Phytopathology®
Verified
Journal Article
Closed access
USUtsala Shrestha
Utsala Shrestha
BOBonnie H. Ownley
Bonnie H. Ownley
ABAlexander Bruce
Alexander Bruce
ERErin Rosskopf
Erin Rosskopf
DBDavid M Butler
David M Butler
A meta-analysis of anaerobic soil disinfestation (ASD) efficacy against Fusarium oxysporum and F. oxysporum f. sp. lycopersici was conducted emphasizing effects of environment and organic amendment characteristics and pot and field studies conducted on ASD amendment C:N ratio and soil temperature effects on F. oxysporum f. sp. lycopersici inoculum survival. In a pot study, two organic amendments, dry molasses-based or wheat bran-based, applied at 4 mg of C/g of soil, with 40:1, 30:1, 20:1, and 10:1 C:N ratios, were evaluated against F. oxysporum f. sp. lycopersici at 15 to 25°C. This study was followed by a pot study with temperature regimes of 15 to 25°C and 25 to 35°C and two C:N ratios (20:1 and 40:1), and a field study at 40:1, 30:1, 20:1, and 10:1 C:N ratios, a 30:1 C:N ratio at a lower C rate (2 mg of C/g of soil), and an anaerobic control. Soil temperature >25°C and more labile amendments increased ASD suppression of F. oxysporum/F. oxysporum f. sp. lycopersici in the meta-analysis. In pot studies, F. oxysporum f. sp. lycopersici survival was reduced for molasses-based mixtures at 20:1 and 30:1 C:N ratios compared with wheat bran-based mixtures but not compared with the anaerobic control. At 25 to 35°C, all ASD treatments suppressed F. oxysporum f. sp. lycopersici relative to controls. In the field, all ASD treatments reduced F. oxysporum f. sp. lycopersici survival compared with the anaerobic control, and 4 mg of C/g of soil amendment rates induced higher anaerobic conditions and higher F. oxysporum f. sp. lycopersici mortality compared with the 2 mg of C/g of soil rate. Although amendment C:N ratios from 10 to 40:1 were similarly suppressive of F. oxysporum, lower temperatures reduced ASD effectiveness against F. oxysporum/F. oxysporum f. sp. lycopersici and further work is warranted to enhance suppression at soil temperatures <25°C.


5 February 2013 in Journal of Agriculture and Environment
Verified
Journal Article
Open access
PDF file
USUtsala Shrestha
Utsala Shrestha
Community participation plays vital role in the development of capacity for the management and utilization of their resources in sustainable way. There are ample success stories for proper management of common property resources through community management. Wetland resources are widely used by the local communities for their sustenance and economic well-being. In such case, local participation is effective tool for establishing an ecologically balanced use of available land and water resources. The participation of the wetland users is crucial for extenuating the problems related to wetland. This paper includes some ideas about community participation and role of local people in wetland conservation and management, reason for conservation of wetland sites, wetland policy based on local participation, and approaches for ensuring participation in wetland conservation programs. The Journal of Agriculture and Environment Vol:12, Jun.2011, Page 140-147 DOI: http://dx.doi.org/10.3126/aej.v12i0.7574