Insufficient or suboptimal dietary intake of iron (Fe) and zinc (Zn) represent a latent health issue affecting a large proportion of the global population, particularly among young children and women living in poor regions at high risk of malnutrition. Agronomic crop biofortification, which consists of increasing the accumulation of target nutrients in edible plant tissues through fertilization or other eliciting factors, has been proposed as a short-term approach to develop functional staple crops and vegetables to address micronutrient deficiency. The aim of the presented study was to evaluate the potential for biofortification of Brassicaceae microgreens through Zn and Fe enrichment. The effect of nutrient solutions supplemented with zinc sulfate (Exp-1; 0, 5, 10, 20 mg L⁻¹) and iron sulfate (Exp-2; 0, 10, 20, 40 mg L⁻¹) was tested on the growth, yield, and mineral concentration of arugula, red cabbage, and red mustard microgreens. Zn and Fe accumulation in all three species increased according to a quadratic model. However, significant interactions were observed between Zn or Fe level and the species examined, suggesting that the response to Zn and Fe enrichment was genotype specific. The application of Zn at 5 and 10 mg L⁻¹ resulted in an increase in Zn concentration compared to the untreated control ranging from 75% to 281%, while solutions enriched with Fe at 10 and 20 mg L⁻¹ increased Fe shoot concentration from 64% in arugula up to 278% in red cabbage. In conclusion, the tested Brassicaceae species grown in soilless systems are good targets to produce high quality Zn and Fe biofortified microgreens through the simple manipulation of nutrient solution composition.

The loss of methyl bromide as a soil fumigant and minimal advances in the development and registration of new chemical fumigants has resulted in a resurgence of interest in the application of organic amendments (OAs) for soilborne plant pathogen and plant-parasitic nematode management. Significant progress has been made in the characterization of OAs, application of strategies for their use, and elucidation of mechanisms by which they suppress soilborne pests. Nonetheless, their utility is limited by the variability of disease control, expense, and the logistics of introducing them into crop production systems. Recent advances in molecular techniques have led to significant progress in the elucidation of the role of bacteria and fungi and their metabolic products on disease suppression with the addition of OAs. Biosolarization and anaerobic soil disinfestation, developed to manipulate systems and favor beneficial microorganisms to maximize their impact on plant pathogens, are built on a strong historical research foundation in OAs and the physical, chemical, and biological characteristics of disease-suppressive soils. This review focuses on recent applications of OAs and their potential for the management of soilborne plant pathogens and plant-parasitic nematodes, with emphasis primarily on annual fruit and vegetable production systems.

A meta-analysis of anaerobic soil disinfestation (ASD) efficacy against Fusarium oxysporum and F. oxysporum f. sp. lycopersici was conducted emphasizing effects of environment and organic amendment characteristics and pot and field studies conducted on ASD amendment C:N ratio and soil temperature effects on F. oxysporum f. sp. lycopersici inoculum survival. In a pot study, two organic amendments, dry molasses-based or wheat bran-based, applied at 4 mg of C/g of soil, with 40:1, 30:1, 20:1, and 10:1 C:N ratios, were evaluated against F. oxysporum f. sp. lycopersici at 15 to 25°C. This study was followed by a pot study with temperature regimes of 15 to 25°C and 25 to 35°C and two C:N ratios (20:1 and 40:1), and a field study at 40:1, 30:1, 20:1, and 10:1 C:N ratios, a 30:1 C:N ratio at a lower C rate (2 mg of C/g of soil), and an anaerobic control. Soil temperature >25°C and more labile amendments increased ASD suppression of F. oxysporum/F. oxysporum f. sp. lycopersici in the meta-analysis. In pot studies, F. oxysporum f. sp. lycopersici survival was reduced for molasses-based mixtures at 20:1 and 30:1 C:N ratios compared with wheat bran-based mixtures but not compared with the anaerobic control. At 25 to 35°C, all ASD treatments suppressed F. oxysporum f. sp. lycopersici relative to controls. In the field, all ASD treatments reduced F. oxysporum f. sp. lycopersici survival compared with the anaerobic control, and 4 mg of C/g of soil amendment rates induced higher anaerobic conditions and higher F. oxysporum f. sp. lycopersici mortality compared with the 2 mg of C/g of soil rate. Although amendment C:N ratios from 10 to 40:1 were similarly suppressive of F. oxysporum, lower temperatures reduced ASD effectiveness against F. oxysporum/F. oxysporum f. sp. lycopersici and further work is warranted to enhance suppression at soil temperatures <25°C.

The adoption of anaerobic soil disinfestation (ASD), a biologically-based method for the management of soilborne pests and pathogens at the commercial scale strictly depends on the availability of effective and low-cost sources of carbon (C). A three-phase pot study was conducted to evaluate the performance of twelve cover crop species as alternative sources of C in comparison to molasses. Buckwheat produced the greatest above-ground and total plant dry biomass and accumulated the largest amount of total C. In the second phase, simulating the application of ASD in a pot-in-pot system, molasses-amended soil achieved substantially higher levels of anaerobicity, and lowered soil pH at 3 and 7 days after treatment application compared to soil amended with the cover crops tested. In the third phase of the study, after the ASD simulation, lettuce was planted to assess the impact of cover crops and molasses-based ASD on lettuce yield and quality. The treatments had limited effects on lettuce plant growth and quality as none of the treatments caused plant stunting or phytotoxicity. Tested cover crop species and molasses had a significant impact on the availability of macro and micro-elements in the soil, which in turn influenced the uptake of minerals in lettuce. Fast growing cover crops like buckwheat or oat, capable of accumulating high levels of C in a relatively short time, may represent a viable alternative to substitute or be combined with standard C sources like molasses, which could provide an on-farm C source and reduce cost of application. Further research is needed to assess the performance of cover crops at the field scale and verify their decomposability and efficacy in managing soil-borne pests and pathogens.