Carbon assimilation is reduced by stress. Under such conditions, the trade-off between growth and non-structural carbohydrate (NSC) storage becomes crucial for plant survival and continued growth. However, growth and NSC responses to drought and shading in Pinus massoniana Lamb. remain unclear. Here, we investigated the effects of drought, shading, and combined drought and shading on leaf gas exchange parameters, stem basal diameter, plant height, biomass accumulation, and NSC concentration in 2-year old seedlings after a 2 month treatment. The results showed that (1) both drought and shading significantly reduced photosynthetic rate, increment of stem basal diameter and plant height, and biomass accumulation, while NSC concentration increased under drought but decreased under shading; (2) the combined drought-shading treatment had a stronger effect on photosynthetic rate and growth than either stress factor individually, whereas the concentration of NSC did not change significantly; and (3) drought, shading, and their combination had a lower effect on biomass than on NSC partitioning, in which case clear effects were observed. Drought increased NSC proportion in roots by 5.4%; conversely, shading increased NSC proportion in leaves by 3.7%, while the combined treatment increased NSC proportion in roots by 5.1% but decreased it in the leaves by 5.4%. These results suggest that the mechanism inhibiting P. massoniana growth is different under drought and shading conditions according to carbon partitioning. Furthermore, complex environmental stress may lead to different mechanisms of carbon partitioning compared with either dry or shaded environments. Our findings will be helpful in predicting the impact of climate change on P. massoniana growth.

Low light and drought often limit the growth and performance of Masson pines (Pinus massoniana) in the subtropical forest ecosystem of China. We speculated that stress-induced defensive secondary metabolites, such as flavonoids and terpenoids, might influence the growth of Masson pines, considering the existence of tradeoffs between growth and defense. However, the mechanisms of Masson pines responsive to low light and drought at the levels of these two metabolites remain unclear. In the present work, the compositions of flavonoids and terpenoids, as well as their biosynthetic pathways, were revealed through metabolome and transcriptome analyses, respectively, coupled with a study on carbon allocation using a ¹³CO₂-pulse-labeling experiment in two-year-old seedlings under low light (LL), drought (DR), and their combined stress (DL) compared to a control (CK). A total of 35 flavonoids and derivatives (LL vs. CK: 18; DR vs. CK: 20; and DL vs. CK: 18), as well as 29 terpenoids and derivatives (LL vs. CK: 23; DR vs. CK: 13; and DL vs. CK: 7), were differentially identified in the leaves. Surprisingly, most of them were decreased under all three stress regimes. At the transcriptomic level, most or all of the detected DEGs (differentially expressed genes) involved in the biosynthetic pathways of flavonoids and terpenoids were downregulated in phloem and xylem under stress treatments. This indicated that stress treatments limited the production of flavonoids and terpenoids. The reduction in the ¹³C allocation to stems might suggest that it is necessary for maintaining the growth of Masson pine seedlings at the whole-plant level by attenuating energetic resources to the biosynthetic pathways of flavonoids and terpenoids when facing the occurrence of adverse environments. Our results provide new insight into understanding the acclimation strategy of Masson pines or other conifers in adverse environments.

The physiological mechanisms driving treeline formation succession captured the attention of ecologists many years ago, yet they are still not fully understood. In this study, physiological parameters (soluble sugars, starch, and nitrogen) were investigated in combination with transcriptomic analysis in the treeline tree species Picea crassifolia. The study was conducted in the middle of Qilian Mountain Reserves, Gansu Province, China, within the elevation range of 2500–3300 m. The results showed that the concentrations of non-structural carbohydrates decreased with increasing elevation in the current-year needles and current-year branches, as well as in the coarse and fine roots. RNA-Seq demonstrated that 483 genes were upregulated and 681 were downregulated in the comparison of 2900 and 2500 m (2900 vs. 2500), 770 were upregulated and 1006 were downregulated in 3300 vs. 2500, and 282 were upregulated and 295 were downregulated in 3300 vs. 2900. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the differentially expressed genes were highly enriched in photosynthesis-related processes, carbon fixation and metabolism, and nitrogen metabolism. Furthermore, almost all photosynthesis-related genes were downregulated, whereas many genes involved in cuticle lipids and flavonoid biosynthesis were upregulated, contributing to the survival of P. crassifolia under the treeline condition. Thus, our study provided not only molecular evidence for carbon limitation hypothesis in treeline formation, but also a better understanding of the molecular mechanisms of treeline tree survival under adverse conditions.

Photosynthesis and the allocation of photosynthetic products are the two main factors that determine plant growth. To understand the growth and productivity of Pinus massoniana Lamb., the diurnal changes in photosynthetic rate were continuously monitored. Furthermore, the translocation and allocation of the photosynthetic products synthesized in the morning and afternoon were explored using ¹³C pulse labeling. The results showed that: (1) on sunny days, the diurnal variation of the net photosynthetic rate showed a “double peak” curve, with an obvious “a depression” when temperatures were highest and humidity lowest. On cloudy days, it showed an irregular “jagged” curve, which was curve consistent with the variations in photosynthetically active radiation (PAR). Meanwhile, the photosynthetic rate changed with the transient changes in environmental factors such as PAR, temperature, and humidity. (2) The mean value of the net photosynthetic rate in the morning was higher than in the afternoon, and the response of the net photosynthetic rate to environmental change (PAR, temperature, humidity, and CO₂ concentration) in the morning was greater than that in the afternoon. (3) The translocation of photosynthetic products synthesized in the afternoon was faster than that in the morning. Shortly after synthesis of photosynthetic products, the translocation of products synthesized in the morning tended toward upper organs (including current-year leaves and 1-year leaves), while the translocation of products synthesized in the afternoon decreased in the upper organs. However, after 15 days of ¹³C pulse labeling, the allocation of the photosynthetic products synthesized in the morning and afternoon tended to be the same. These results indicate that the differences in the photosynthetic products synthesized and the temporal differences in the translocation rates did not affect the final allocation of the photosynthetic products in the various organs of the P. massoniana. These results improve our knowledge of the functional phases of P. massoniana during the diurnal cycle.

Investigating the mechanisms by which plants adapt to low phosphorus content in ecosystems is crucial for nutrient dynamics division. Our study investigated the growth adaptation strategies of Pinus massoniana seedlings to low phosphorus conditions, including nutrient and non-structural carbohydrate (NSC) allocation, nutrient stoichiometry, and changes in nutrient resorption efficiency along a fact-based gradient. Our results showed that the total biomass and aboveground biomass proportion increased with substrate phosphorus content, reaching maximum biomass in the one-time phosphorus treatment. The nutrient concentration of components remained relatively stable, with the allocating preference to roots and needles under low phosphorus conditions. NSC was allocated as starch in fine roots and as soluble sugar in needles. Seedlings did not show signs of phosphorus limitation, even in the non-phosphorus group. The nitrogen resorption efficiency to phosphorus resorption efficiency ratio (NRE: PRE) of needles significantly varied between the high and low phosphorus treatments. In response to phosphorus deficiency, seedlings demonstrated homeostatic adjustments to maintain the relative stability of nutrient concentration. Fine roots and needles were prioritized to ensure nutrient uptake and photosynthetic product production. Additionally, it was necessary to differentiate the indicative function of nitrogen/phosphorus for various species and components, and NRE: PRE potentially provides a sensitive indicator of nutrient limitation status.