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H. Mighri
Separation, Analysis and Valorization of Active Natural Products Laboratory, Arid Regions Institute, Gabes University, Medenine, Tunisia

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Journal article
Published: 17 March 2021 in Preparative Biochemistry & Biotechnology
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This study was carried out to evaluate the effect of extraction methods (direct maceration (DM) and successive maceration (SM)) and solvents (dichloromethane (DCM), ethyl acetate (EAc), butanol (But), and aqueous extraction (Aqu)) on the phenolic composition and biological activities of Moringa oleifera leaves cultivated for the first time in Tunisia. Total polyphenol content (TPC), total flavonoid content (TFC) and LC-MS analysis were performed for all extracts. Antioxidant potential by DPPH, metal chelating, and FRAP assays, antibacterial activity against Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922) by the minimum inhibitory concentration method (MIC) and cytotoxic properties against lung cancer cells (A549), were determined. Phenolic compounds and biological activities of M. oleifera leaves depend on the method and the solvent used for the extraction of these bioactive substances. The But extract prepared by SM method exhibited the highest amounts of TPC (103.06 ± 0.5 mg GAE/g DE) and showed the strongest potential antioxidant (CI50 = 0.26 mg/mL on DPPH test), antibacterial (MIC = 250 µg/mL) and cytotoxic activities (GI50 = 69.9 µg/mL). LC-MS analysis showed that 28 phenolic compounds of 33 tested standards were found in M. oleifera leaves. The But extract obtained by SM method exhibited the highest amounts of rutin, quercetin and syringic acid.

ACS Style

Naima Bennour; Hedi Mighri; Talel Bouhamda; Mahmoud Mabrouk; Elif Apohan; Ozfer Yesilada; Hasan Küçükbay; Ahmed Akrout. Moringa oleifera leaves: could solvent and extraction method affect phenolic composition and bioactivities? Preparative Biochemistry & Biotechnology 2021, 1 -8.

AMA Style

Naima Bennour, Hedi Mighri, Talel Bouhamda, Mahmoud Mabrouk, Elif Apohan, Ozfer Yesilada, Hasan Küçükbay, Ahmed Akrout. Moringa oleifera leaves: could solvent and extraction method affect phenolic composition and bioactivities? Preparative Biochemistry & Biotechnology. 2021; ():1-8.

Chicago/Turabian Style

Naima Bennour; Hedi Mighri; Talel Bouhamda; Mahmoud Mabrouk; Elif Apohan; Ozfer Yesilada; Hasan Küçükbay; Ahmed Akrout. 2021. "Moringa oleifera leaves: could solvent and extraction method affect phenolic composition and bioactivities?" Preparative Biochemistry & Biotechnology , no. : 1-8.

Journal article
Published: 01 October 2019 in Antioxidants
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Five Oudneya Africana (OA) leaves extracts were screened for their total phenolic (TPC), total flavonoid (TFC), condensed tannins (CTC) content, as well as their antioxidant capacity. The highest amount of TPC (661.66 ± 0.08 mg GAE/g), TFC (344.68 ± 0.44 mg QE/g) and TCT (90.18 ± 0.49 mg CE/g) was recorded to ethanol, acetone, and dichloromethane extracts, respectively. For 2,2-diphenyl-1-picrylhydrazyl (DPPH) (22.00 ± 0.03 µg/mL) and Reducing Power Assay (FRAP) (269.00 ± 0.01µg/mL) assays, ethanol extract showed the potent activity, while with ABTS test, acetone extract was the most active (761.15 ± 0.09 µg/mL). HPLC-MS analysis of acetonic and ethanolic extracts reveals the predominance of quinic acid, chlorogenic acid, 4‐O‐caffeoylquinic acid, and rutin compounds. The addition effect evaluation of OA extracts in beef burger preservation demonstrates the powerful effect (p < 0.05) of acetonic and ethanolic ones (0.03%) to inhibit lipids oxidation during storage for 10 days, given by the lowest increase in Thiobarbituric Acid-reactive Substances (TBARS) values as compared to the (−) control with a significant difference between free thiols values. In addition, these two extracts appear to be effective (p < 0.05) for pH stability, color, and sensory parameters as compared to (+) and (−) controls and aqueous extract. Hamburger odour was considered as a dependent variable in multiple linear regression analysis, where the models results showed that physicochemical parameters determine more burger odour than sensorial ones.

ACS Style

Hafedh Hajlaoui; Soumaya Arraouadi; Hedi Mighri; Mouna Chaaibia; Néji Gharsallah; Gaspar Ros; Gema Nieto; And Adel Kadri; Adel Kadri; Ros. Phytochemical Constituents and Antioxidant Activity ofOudneya Africana L. Leaves Extracts: Evaluation Effects on Fatty Acids and Proteins Oxidation of Beef Burger during Refrigerated Storage. Antioxidants 2019, 8, 442 .

AMA Style

Hafedh Hajlaoui, Soumaya Arraouadi, Hedi Mighri, Mouna Chaaibia, Néji Gharsallah, Gaspar Ros, Gema Nieto, And Adel Kadri, Adel Kadri, Ros. Phytochemical Constituents and Antioxidant Activity ofOudneya Africana L. Leaves Extracts: Evaluation Effects on Fatty Acids and Proteins Oxidation of Beef Burger during Refrigerated Storage. Antioxidants. 2019; 8 (10):442.

Chicago/Turabian Style

Hafedh Hajlaoui; Soumaya Arraouadi; Hedi Mighri; Mouna Chaaibia; Néji Gharsallah; Gaspar Ros; Gema Nieto; And Adel Kadri; Adel Kadri; Ros. 2019. "Phytochemical Constituents and Antioxidant Activity ofOudneya Africana L. Leaves Extracts: Evaluation Effects on Fatty Acids and Proteins Oxidation of Beef Burger during Refrigerated Storage." Antioxidants 8, no. 10: 442.

Journal article
Published: 10 June 2019 in South African Journal of Botany
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The aim of the study was to identify the best evaporation process that allows obtaining a dry methanol extract of Moringa oleifera leaves rich on phytochemicals and showing a significant antioxidant activity. The liquid extract, prepared by the maceration of the leaves of M. oleifera into 70% aqueous methanol at room temperature, was evaporated using three solvent evaporation methods, these included evaporation in air at room temperature, with rotary evaporator and in an oven at 40 °C. Extracts were investigated for total polyphenols content (TPC), total flavonoids content (TFC), total condensed tannins content (TCTC), identification and quantification of some phenolic compounds by high-performance liquid chromatography coupled with photodiode array and mass spectrometry detection (HPLC-PDA-ESI/MS) and evaluated for antioxidant activity. The analysis of extracts by HPLC-PDA-ESI/MS showed that the dry extract obtained in dark after oven evaporation exhibited the highest amounts of 13 out of 19 phenolic compounds; quinic acid, gallic acid, 4-O-caffeoylquinic acid, caffeic acid, trans-ferulic acid, apigenin 7-O-glucoside, quercetrin, kaempferol, naringenin, apigenin, luteolin, cirsiliol and cirsilineol. In all extracts, quinic acid, 4-O-caffeoylquinic acid, rutin and quercitrin were present as the main compounds. The chemical composition and the antioxidant potential of the methanol extract are strongly affected by the method of evaporation of the solvent used. The highest values of TPC, TCTC and the antioxidant activity were provided by the extract obtained when the solvent was evaporated in oven. These results showed that the evaporation of the solvent in the oven at 40 °C was the best evaporation method to be used for the extraction of phytochemicals from the leaves of M. oleifera by cold maceration method using 70% aqueous methanol as solvent.

ACS Style

N. Bennour; H. Mighri; H. Eljani; T. Zammouri; A. Akrout. Effect of solvent evaporation method on phenolic compounds and the antioxidant activity of Moringa oleifera cultivated in Southern Tunisia. South African Journal of Botany 2019, 129, 181 -190.

AMA Style

N. Bennour, H. Mighri, H. Eljani, T. Zammouri, A. Akrout. Effect of solvent evaporation method on phenolic compounds and the antioxidant activity of Moringa oleifera cultivated in Southern Tunisia. South African Journal of Botany. 2019; 129 ():181-190.

Chicago/Turabian Style

N. Bennour; H. Mighri; H. Eljani; T. Zammouri; A. Akrout. 2019. "Effect of solvent evaporation method on phenolic compounds and the antioxidant activity of Moringa oleifera cultivated in Southern Tunisia." South African Journal of Botany 129, no. : 181-190.

Journal article
Published: 15 May 2019 in South African Journal of Botany
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Ephedra alata is a medicinal plant with a long history of traditional use for its bronchodilator, anti-asthmatic and cytotoxic effects. In this work, we develop a sensitive and validate LC-ESI/MS method to simultaneously identify and quantify 33 standard phenolic compounds in E. alata methanol (MeOH) crude extract and its derived fractions (Dichloromethane (DCM), Ethyl acetate (EAc), butanol (BuOH) and water). The antioxidant activities were tested using total antioxidant capacity (TAC), DPPH and FRAP assays. Results showed that the validated and reliable LC-ESI/MS method used unambiguously lead to identify and quantify 24 phenolic compounds most of them are present in all extracts. The major components detected were epicatechin, quinic acid, quercetin-3-o-rhamnoside, catechin, trans-cinnamic acid, naringin and trans-ferulic acid which their amounts vary according to the solvent used for the extraction. The DCM extract showed the highest activity when the TAC test was applied, the BuOH and EAc extracts displayed the highest activities with the DPPH test, whereas the DCM and EAc extracts exhibited the strongest activities with the FRAP method. This difference could be attributed to the nature and amounts of phenolic compounds of the extracts. These realized phytochemical and biological analyses could be helpful for the potential use of this plant in novel medicament products.

ACS Style

H. Mighri; A. Akrout; N. Bennour; H. Eljeni; T. Zammouri; M. Neffati. LC/MS method development for the determination of the phenolic compounds of Tunisian Ephedra alata hydro-methanolic extract and its fractions and evaluation of their antioxidant activities. South African Journal of Botany 2019, 124, 102 -110.

AMA Style

H. Mighri, A. Akrout, N. Bennour, H. Eljeni, T. Zammouri, M. Neffati. LC/MS method development for the determination of the phenolic compounds of Tunisian Ephedra alata hydro-methanolic extract and its fractions and evaluation of their antioxidant activities. South African Journal of Botany. 2019; 124 ():102-110.

Chicago/Turabian Style

H. Mighri; A. Akrout; N. Bennour; H. Eljeni; T. Zammouri; M. Neffati. 2019. "LC/MS method development for the determination of the phenolic compounds of Tunisian Ephedra alata hydro-methanolic extract and its fractions and evaluation of their antioxidant activities." South African Journal of Botany 124, no. : 102-110.

Journal article
Published: 28 March 2012 in International Journal of Life Science and Medical Research
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ACS Style

Ahmed Akrout; Hédi Mighri; Mabrouka Krid; Fatma Thabet; Hamida Turki; Mohammed Neffati. Chemical Composition and Antioxidant Activity of Aqueous Extracts of Some Medicinal Plants Wild Growing in Southern Tunisia. International Journal of Life Science and Medical Research 2012, 2, 1 -4.

AMA Style

Ahmed Akrout, Hédi Mighri, Mabrouka Krid, Fatma Thabet, Hamida Turki, Mohammed Neffati. Chemical Composition and Antioxidant Activity of Aqueous Extracts of Some Medicinal Plants Wild Growing in Southern Tunisia. International Journal of Life Science and Medical Research. 2012; 2 (1):1-4.

Chicago/Turabian Style

Ahmed Akrout; Hédi Mighri; Mabrouka Krid; Fatma Thabet; Hamida Turki; Mohammed Neffati. 2012. "Chemical Composition and Antioxidant Activity of Aqueous Extracts of Some Medicinal Plants Wild Growing in Southern Tunisia." International Journal of Life Science and Medical Research 2, no. 1: 1-4.

Journal article
Published: 12 November 2010 in Chemistry & Biodiversity
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The intraspecific chemical variability of essential oils (50 samples) isolated from the aerial parts of Artemisia herba-alba Asso growing wild in the arid zone of Southeastern Tunisia was investigated. Analysis by GC (RI) and GC/MS allowed the identification of 54 essential oil components. The main compounds were β-thujone and α-thujone, followed by 1,8-cineole, camphor, chrysanthenone, trans-sabinyl acetate, trans-pinocarveol, and borneol. Chemometric analysis (k-means clustering and PCA) led to the partitioning into three groups. The composition of two thirds of the samples was dominated by α-thujone or β-thujone. Therefore, it could be expected that wild plants of A. herba-alba randomly harvested in the area of Kirchaou and transplanted by local farmers for the cultivation in arid zones of Southern Tunisia produce an essential oil belonging to the α-thujone/β-thujone chemotype and containing also 1,8-cineole, camphor, and trans-sabinyl acetate at appreciable amounts.

ACS Style

Hédi Mighri; Ahmed Akrout; Hajer El‐Jeni; Slah Zaidi; Félix Tomi; Joseph Casanova; Mohamed Neffati. Composition and Intraspecific Chemical Variability of the Essential Oil from Artemisia herba-alba Growing Wild in a Tunisian Arid Zone. Chemistry & Biodiversity 2010, 7, 2709 -2717.

AMA Style

Hédi Mighri, Ahmed Akrout, Hajer El‐Jeni, Slah Zaidi, Félix Tomi, Joseph Casanova, Mohamed Neffati. Composition and Intraspecific Chemical Variability of the Essential Oil from Artemisia herba-alba Growing Wild in a Tunisian Arid Zone. Chemistry & Biodiversity. 2010; 7 (11):2709-2717.

Chicago/Turabian Style

Hédi Mighri; Ahmed Akrout; Hajer El‐Jeni; Slah Zaidi; Félix Tomi; Joseph Casanova; Mohamed Neffati. 2010. "Composition and Intraspecific Chemical Variability of the Essential Oil from Artemisia herba-alba Growing Wild in a Tunisian Arid Zone." Chemistry & Biodiversity 7, no. 11: 2709-2717.

Original articles
Published: 01 January 2010 in Journal of Essential Oil Bearing Plants
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The essential oils extracted by Clavenger apparatus from leaves of Rosmarinus officinalis cultivated in different areas in Djerba (Island in the southern of Tunisia) were evaluated for their chemical composition (GC and GC-MS methods), antioxidant (DPPH method) and antibacterial activities (agar-well diffusion method). GC-MS analysis revealed the presence of three chemotypes of oils: 1,8-cineole, 1,8-cineole/camphor/α-pinene/camphene and 1,8-cineole/camphor/α-pinene/ verbenone/borneol which was not previously detected in Tunisian Rosmarinus officinalis. The three chemtypes exhibited moderate antioxidant activity with an IC50 ranged from 4.186 mg/ml for chemotype I to 7.298 for chemotype II and showed strong to moderate antibacterial activity against the six bacterial strains tested with a MIC ranged from 0.156 to 1.25 mg/mL (Chemotype I and chemotype II) and from 1.25 to 5 mg/mL (chemotype III). This study showed that the essential oil extracted from Rosmarinus officinalis cultivated in Djerba has similar chemical composition and biological activities as essential oil isolated from wild growing Rosmarinus officinalis.

ACS Style

Ahmed Akrout; Hafedh Hajlaoui; Hédi Mighri; Hanene Najjaa; Hajer El Jani; Slah Zaidi; Mohamed Neffati. Chemical and Biological Characteristics of Essential Oil ofRosmarinus officinalisCultivated in Djerba. Journal of Essential Oil Bearing Plants 2010, 13, 398 -411.

AMA Style

Ahmed Akrout, Hafedh Hajlaoui, Hédi Mighri, Hanene Najjaa, Hajer El Jani, Slah Zaidi, Mohamed Neffati. Chemical and Biological Characteristics of Essential Oil ofRosmarinus officinalisCultivated in Djerba. Journal of Essential Oil Bearing Plants. 2010; 13 (4):398-411.

Chicago/Turabian Style

Ahmed Akrout; Hafedh Hajlaoui; Hédi Mighri; Hanene Najjaa; Hajer El Jani; Slah Zaidi; Mohamed Neffati. 2010. "Chemical and Biological Characteristics of Essential Oil ofRosmarinus officinalisCultivated in Djerba." Journal of Essential Oil Bearing Plants 13, no. 4: 398-411.

Original articles
Published: 01 September 2009 in Journal of Essential Oil Research
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Seedlings of Artemisia herba-alba Asso collected from Kirchaou area were transplanted in an experimental garden near the Institut des Régions Arides of Médenine (Tunisia). During three years, the aerials parts were harvested (three levels of cutting, 25%, 50% and 75% of the plant), at full blossom and during the vegetative stage. The essential oil was isolated by hydrodistillation and its chemical composition was determined by GC(RI) and 13C-NMR. With respect to the quantity of vegetable material and the yield of hydrodistillation, it appears that the best results were obtained for plants cut at 50% of their height and during the full blossom. The chemical composition of the essential oil was dominated by β-thujone, α-thujone, 1,8-cineole, camphor and trans-sabinyl acetate, irrespective of the level of cutting and the period of harvest. It remains similar to that of plants growing wild in the same area.

ACS Style

Hédi Mighri; Ahmed Akrout; Mohamed Neffati; Félix Tomi; Joseph Casanova. The Essential Oil FromArtemisia herba-albaAsso Cultivated in Arid Land (South Tunisia). Journal of Essential Oil Research 2009, 21, 453 -456.

AMA Style

Hédi Mighri, Ahmed Akrout, Mohamed Neffati, Félix Tomi, Joseph Casanova. The Essential Oil FromArtemisia herba-albaAsso Cultivated in Arid Land (South Tunisia). Journal of Essential Oil Research. 2009; 21 (5):453-456.

Chicago/Turabian Style

Hédi Mighri; Ahmed Akrout; Mohamed Neffati; Félix Tomi; Joseph Casanova. 2009. "The Essential Oil FromArtemisia herba-albaAsso Cultivated in Arid Land (South Tunisia)." Journal of Essential Oil Research 21, no. 5: 453-456.