Background: Breeding for new maize varieties with propitious root systems has tremendous potential in improving water and nutrients use efficiency and plant adaptation under suboptimal conditions. To date, most of the previously detected root-related trait genes in maize were new without functional verification. In this study, seven seedling root architectural traits were examined at three developmental stages in a recombinant inbred line population (RIL) of 179 RILs and a genome-wide association study (GWAS) panel of 80 elite inbred maize lines through quantitative trait loci (QTL) mapping and genome-wide association study. Results: Using inclusive composite interval mapping, 8 QTLs accounting for 6.44–8.83 % of the phenotypic variation in root traits, were detected on chromosomes 1 (qRDW_v3-1-1 and qRDW/SDW_v3-1-1), 2 (qRBN_v1-2-1), 4 (qSUA_v1-4-1, qSUA_v2-4-1, and qROV_v2-4-1), and 10 (qTRL_v1-10-1, qRBN_v1-10-1). GWAS analysis involved three models (EMMAX, FarmCPU, and MLM) for a set of 1,490,007 high-quality single nucleotide polymorphisms (SNPs) obtained via whole genome next-generation sequencing (NGS). Overall, 53 significant SNPs with a phenotypic contribution rate ranging from 5.10 to 30.2 % and spread all over the ten maize chromosomes exhibited associations with the seven root traits. 17 SNPs were repeatedly detected from at least two growth stages, with several SNPs associated with multiple traits stably identified at all evaluated stages. Within the average linkage disequilibrium (LD) distance of 5.2 kb for the significant SNPs, 46 candidate genes harboring substantial SNPs were identified. Five potential genes viz. Zm00001d038676, Zm00001d015379, Zm00001d018496, Zm00001d050783, and Zm00001d017751 were verified for expression levels using maize accessions with extreme root branching differences from the GWAS panel and the RIL population. The results showed significantly (P < 0.001) different expression levels between the outer materials in both panels and at all considered growth stages. Conclusions: This study provides a key reference for uncovering the complex genetic mechanism of root development and genetic enhancement of maize root system architecture, thus supporting the breeding of high-yielding maize varieties with propitious root systems.

Root systems can display variable genetic architectures leading to nutrient foraging or improving abiotic stress tolerance. Breeding for new soybean varieties with efficient root systems has tremendous potential in enhancing resource use efficiency and plant adaptation for challenging climates. In this study, root related traits were analyzed in a panel of 260 spring soybean with genome-wide association study (GWAS). Genotyping was done with specific locus amplified fragment sequencing (SLAF-seq), and five GWAS models (GLM, MLM, CMLM, FaST-LMM, and EMMAX) were used for analysis. A total of 179,960 highly consistent SNP markers distributed over the entire genome with an inter-marker distance of 2.36 kb was used for GWAS analysis. Overall, 27 significant SNPs with a phenotypic contribution ranging from 20 to 72% and distributed on chromosomes 2, 6, 8, 9, 13, 16 and 18 were identified and two of them were found to be associated with multiple root-related traits. Based on the linkage disequilibrium (LD) distance of 9.5 kb for the different chromosomes, 11 root and shoot regulating genes were detected based on LD region of a maximum 55-bp and phenotypic contribution greater than 22%. Expression analysis revealed an association between expression levels of those genes and the degree of root branching number. The current study provides new insights into the genetic architecture of soybean roots, and the underlying SNPs/genes could be critical for future breeding of high-efficient root system in soybean.

The results of numerous scientific researches in developed countries shows that the content produced by social media has a significant impact on the behavior and lifestyle of young people, especially in developing countries such as Afghanistan; But the main problem is that there has been no serious awareness and cognition about the harmful impact of social media content on young people on developing countries, which is comprehensively discussed in this research paper. The general purpose of this paper is to provide information and create awareness about the negative impact of social media on youth's social behavior through content analyses and qualitative research. Social Media refers to different types of platforms or applications such as WhatsApp, Facebook, TikTok, Twitter, LinkedIn, YouTube, etc. through which people share information and use it as a communication resource.This study has specifically addressed the basic questions that How social media changes youth's social behavior and what are the main factors which because social behavior changes in youths by using social media and what are the negative impact of social media on youth's social lives by using reliable resources.  Even though social media sites and platforms have more advantages and positive impacts and provide a lot of communication, business, and entertainment opportunities, it leads to social isolation, anxiety, depression, cyberbullying, sexuality, loss of privacy, terrorism, etc.  It reduces the number of face-to-face interactions among the youth because they normally spend most of their time on online social platforms. The result of this research shows that social media leads to social isolation and this can cause several effects such as physical, emotional, mental, and psychological issues on youths. This can lead to depression, anxiety, and many other problems.