This page has only limited features, please log in for full access.

Unclaimed
Isabella Muscari
Section of onco-hematology, Department of Medicine, University of Perugia, 06132 Perugia, Italy

Basic Info

Basic Info is private.

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Communication
Published: 22 July 2020 in Molecules
Reads 0
Downloads 0

In previous studies, we isolated the known compound saponin XII from the roots of Dipsacus japonicus Miq. Here, we show that this compound reduced the number of acute myeloid leukemia OCI-AML3 cells as evaluated by a hemocytometer. Flow cytometry analyses demonstrated that the reported activity was associated with a significant increase of apoptosis and of cells in the G0/G1 phase of the cell cycle, with a decrease of cells in the S and G2/M phases. Thus, the inhibition of cell growth in OCI-AML3 cells was due to antiproliferative and pro-apoptotic effects. Interestingly, the bioactivity of saponin XII exerted its effect at a concentration as low as 1 µg/mL.

ACS Style

Ba Thi Cham; Nguyen Thi Thuy Linh; Do Thi Thao; Nguyen Thi Hoang Anh; Nguyen Thanh Tam; Bui Kim Anh; Isabella Muscari; Sabrina Adorisio; Tran Van Sung; Trinh Thi Thuy; Domenico V. Delfino. Cell Growth Inhibition of Saponin XII from Dipsacus japonicus Miq. on Acute Myeloid Leukemia Cells. Molecules 2020, 25, 3325 .

AMA Style

Ba Thi Cham, Nguyen Thi Thuy Linh, Do Thi Thao, Nguyen Thi Hoang Anh, Nguyen Thanh Tam, Bui Kim Anh, Isabella Muscari, Sabrina Adorisio, Tran Van Sung, Trinh Thi Thuy, Domenico V. Delfino. Cell Growth Inhibition of Saponin XII from Dipsacus japonicus Miq. on Acute Myeloid Leukemia Cells. Molecules. 2020; 25 (15):3325.

Chicago/Turabian Style

Ba Thi Cham; Nguyen Thi Thuy Linh; Do Thi Thao; Nguyen Thi Hoang Anh; Nguyen Thanh Tam; Bui Kim Anh; Isabella Muscari; Sabrina Adorisio; Tran Van Sung; Trinh Thi Thuy; Domenico V. Delfino. 2020. "Cell Growth Inhibition of Saponin XII from Dipsacus japonicus Miq. on Acute Myeloid Leukemia Cells." Molecules 25, no. 15: 3325.

Journal article
Published: 29 August 2019 in Toxins
Reads 0
Downloads 0

Cladosporium species are endophytic fungi that grow on organic matter and are considered food contaminants. The anti-microbial and anti-tumor naphthoquinones fusarubin (FUS) and anhydrofusarubin (AFU) were isolated using column chromatography from a Cladosporium species residing inside Rauwolfia leaves. The impact of FUS and AFU on cell growth was assessed in acute myeloid leukemia (OCI-AML3) and other hematologic tumor cell lines (HL-60, U937, and Jurkat). Treatment with FUS or AFU reduced the number of OCI-AML3 cells as evaluated by a hemocytometer. Flow cytometry analyses showed that this effect was accompanied by diverse impairments in cell cycle progression. Specifically, FUS (20 or 10 μg/mL significantly decreased the percentage of cells in S phase and increased the percentage of cells in G2/M phase, whereas AFU increased the percentage of cells in G0/G1 phase (50 and 25 μg/mL) and decreased the percentage of cells in S (50 μg/mL) and G2/M (50 and 25 μg/mL) phases. Both substances significantly increased apoptosis at higher concentrations. The effects of FUS were more potent than those of AFU, with FUS up-regulating p21 expression in a p53-dependent manner, as detected by Western blot analyses, likely the consequence of decreased ERK phosphorylation and increased p38 expression (both of which increase p21 stability). FUS also decreased Akt phosphorylation and resulted in increased Fas ligand production and caspase-8/3-dependent apoptosis. These results suggest that FUS and AFU inhibit proliferation and increase apoptosis in cell lines derived from hematological cancers.

ACS Style

Sabrina Adorisio; Alessandra Fierabracci; Isabella Muscari; Anna Marina Liberati; Lorenza Cannarile; Trinh Thi Thuy; Tran Van Sung; Hossain Sohrab; Choudhury Mahmood Hasan; Emira Ayroldi; Carlo Riccardi; Abdul Mazid; Domenico V. Delfino. Fusarubin and Anhydrofusarubin Isolated from A Cladosporium Species Inhibit Cell Growth in Human Cancer Cell Lines. Toxins 2019, 11, 503 .

AMA Style

Sabrina Adorisio, Alessandra Fierabracci, Isabella Muscari, Anna Marina Liberati, Lorenza Cannarile, Trinh Thi Thuy, Tran Van Sung, Hossain Sohrab, Choudhury Mahmood Hasan, Emira Ayroldi, Carlo Riccardi, Abdul Mazid, Domenico V. Delfino. Fusarubin and Anhydrofusarubin Isolated from A Cladosporium Species Inhibit Cell Growth in Human Cancer Cell Lines. Toxins. 2019; 11 (9):503.

Chicago/Turabian Style

Sabrina Adorisio; Alessandra Fierabracci; Isabella Muscari; Anna Marina Liberati; Lorenza Cannarile; Trinh Thi Thuy; Tran Van Sung; Hossain Sohrab; Choudhury Mahmood Hasan; Emira Ayroldi; Carlo Riccardi; Abdul Mazid; Domenico V. Delfino. 2019. "Fusarubin and Anhydrofusarubin Isolated from A Cladosporium Species Inhibit Cell Growth in Human Cancer Cell Lines." Toxins 11, no. 9: 503.