Biocompatible hydrotalcite nanohybrids, i.e., layered double hydroxide (LDH) based nanohybrids have attracted significant attention for biomedical functions. Benefiting from good biocompatibility, tailored drug incorporation, high drug loading capacity, targeted cellular delivery and natural pH-responsive biodegradability, hydrotalcite nanohybrids have shown great potential in drug/gene delivery, cancer therapy and bio-imaging. This review aims to summarize recent progress of hydrotalcite nanohybrids, including the history of the hydrotalcite-like compounds for application in the medical field, synthesis, functionalization, physicochemical properties, cytotoxicity, cellular uptake mechanism, as well as their related applications in biomedicine. The potential and challenges will also be discussed for further development of LDHs both as drug delivery carriers and diagnostic agents.

Biomedical investigations using layered double hydroxide (LDH) nanoparticles have attracted tremendous attentions due to their advantages such as biocompatibility, variable-chemical compositions, anion-exchange capacity, host–guest interactions, and crystallization-dissolution characters. Bio-imaging becomes more and more important since it allows theranostics to combine therapy and diagnosis, which is a concept of next-generation medicine. Based on the unique features mentioned above, LDHs create novel opportunities for bio-imaging and simultaneous therapy with LDHs-based nanohybrids. This review aims to explore the recent advances in multifunctional LDH nanohybrids ranging from synthesis to practical applications for various bio-imaging with therapeutic functions. Furthermore, their potential both as diagnostic agents and drug delivery carriers will be discussed with the improvement in noninvasive bio-imaging techniques.