To provide a new way to increase water storage and retention of dryland wheat, a field study was conducted at Wenxi experimental site of Shanxi Agricultural University. The effect of subsoiling in fallow period on soil water storage, accumulation of proline, and formation of grain protein after anthesis were determined. Our results showed that subsoiling in fallow period could increase water storage in the 0–300 cm soil at pre-sowing stage and at anthesis stage with low or medium N application, especially for the 60–160 cm soil. However, the proline content, glutamine synthetase (GS) activity, glutamate dehydrogenase (GDH) activity in flag leaves and grains were all decreased by subsoiling in fallow period. In addition, the content of albumin, gliadin, and total protein in grains were also decreased while globulin content, Glu/Gli, protein yield, and glutelin content were increased. With N application increasing, water storage of soil layers from 20 to 200 cm was decreased at anthesis stage. High N application resulted in the increment of proline content and GS activity in grains. Besides, correlation analysis showed that soil storage in 40–160 cm soil was negatively correlated with proline content in grains; proline content in grains was positively correlated with GS and GDH activity in flag leaves. Contents of albumin, globulin and total protein in grains were positively correlated with proline content in grains and GDH activity in flag leaves. In conclusion, subsoiling in fallow period, together with N application at 150 kg·hm⁻², was beneficial to increase the protein yield and Glu/Gli in grains which improve the quality of wheat.

The research conducted at the Shanxi Agricultural University’s Quinoa Experimental Model Base in Jinzhong, Shanxi Province, aimed to assess agronomic traits and their correlation with yield across 32 quinoa varieties. Three distinct yield categories emerged: low (≤1500 kg ha⁻¹), middle (1500–2500 kg⁻¹), and high (>2500 kg ha⁻¹). High-yielding varieties demonstrated notable characteristics, including decreased plant height and increased leaf area per plant at maturity compared to low- and middle-yielding varieties. Moreover, the decline in leaf area per plant and root traits from flowering to maturity was less pronounced in the high-yielding varieties. The high-yielding varieties had a higher hardness of the stem base and middle stem by 12–13.7% and 6.3–11.5% compared to the medium- and low-yield varieties. Furthermore, high-yielding varieties indicated improvements in dry matter accumulation, decreased effective branch number, and increased main ear length and 1000-grain weight. Correlation analysis highlighted significant relationships between grain weight, yield, post-flowering senescence, and root and leaf characteristics. Structural equation model analysis revealed the negative impact of certain root and leaf traits on grain weight and yield, suggesting their importance in determining productivity. Notably, high-yielding varieties exhibited traits conducive to increased grain weight, including shorter plant height, slower root senescence, and enhanced post-flowering leaf resilience. These findings showed that understanding the relationship between agronomic traits and yield potential is crucial for optimizing quinoa production and promoting the sustainable development of this essential crop.

Waxy corn is a special type of maize primarily consumed as a fresh vegetable by humans. Nitrogen (N) plays an essential role in regulating the growth progression, maturation, yield, and quality of waxy maize. A reasonable N application rate is vital for boosting the accumulation of both N and carbon (C) in the grains, thereby synergistically enhancing the grain quality. However, the impact of varying N levels on the dynamic changes in N metabolism, carbohydrate formation, and anthocyanin synthesis in purple waxy corn kernels, as well as the regulatory relationships among these processes, remains unclear. To explore the effects of varying N application rates on the N metabolism, carbohydrate formation, and anthocyanin synthesis in kernels during grain filling, a two-year field experiment was carried out using the purple waxy maize variety Jinnuo20 (JN20). This study examined the different N levels, specifically 0 (N0), 120 (N1), 240 (N2), and 360 (N3) kg N ha⁻¹. The results of the analysis revealed that, for nearly all traits measured, the N application rate of N2 was the most suitable. Compared to the N0 treatment, the accumulation and content of anthocyanins, total nitrogen, soluble sugars, amylopectin, and C/N ratio in grains increased by an average of 35.62%, 11.49%, 12.84%, 23.74%, 13.00%, and 1.87% under N2 treatment over five filling stages within two years, respectively, while the harmful compound nitrite content only increased by an average of 30.2%. Correspondingly, the activities of related enzymes also significantly increased and were maintained under N2 treatment compared to N0 treatment. Regression and correlation analysis results revealed that the amount of anthocyanin accumulation was highly positively correlated with the activities of phenylalanine ammonia-lyase (PAL) and flavanone 3-hydroxylase (F3H), but negatively correlated with anthocyanidin synthase (ANS) and UDP-glycose: flavonoid-3-O-glycosyltransferase (UFGT) activity, nitrate reductase (NR), and glutamine synthetase (GS) showed significant positive correlations with the total nitrogen content and lysine content, and a significant negative correlation with nitrite, while soluble sugars were negatively with ADP-glucose pyrophosphorylase (AGPase) activity, and amylopectin content was positively correlated with the activities of soluble starch synthase (SSS), starch branching enzyme (SBE), and starch debranching enzyme (SDBE), respectively. Furthermore, there were positive or negative correlations among the detected traits. Hence, a reasonable N application rate improves purple waxy corn kernel nutritional quality by regulating N metabolism, as well as carbohydrate and anthocyanin biosynthesis.

To evaluate the effects of long-term fallow tillage on soil microbial community structure in different soil layers and winter wheat yield, we conducted a 5-year long-term field experiment in the Loess Plateau, China, using three fallow tillage methods: no-tillage (NT), subsoiling tillage (ST), and deep plowing (DP). The soil physical and chemical properties, community structure, and composition of soil bacteria and fungi in the 0–20 cm and 20–40 cm soil layers, and winter wheat yield were analyzed. The results showed that, compared with DP, NT and ST significantly increased soil moisture content (SWC), soil organic carbon (SOC) content, and dissolved organic carbon (DOC) contents in 0–20 cm soil layer (p < 0.05), and significantly increased soil microbial community Shannon and Simpson index in 0–40 cm soil layer (p < 0.05). Compared with NT, ST and DP significantly increased SWC and SOC contents in 20–40 cm soil layer (p < 0.05). Actinobacteria and Ascomycota were the most abundant bacteria and fungi in the soil of the experimental site. Redundancy analysis further showed that soil physicochemical properties (SWC, SOC, DOC, and DON) were closely related to the microbial community. PICRUSt2 prediction results showed that DP increased the metabolic functional diversity of bacteria and fungi. ST and DP significantly increased the yield of winter wheat, and DP had the best effect. In conclusion, subsoiling tillage and deep plowing were beneficial to the accumulation and utilization of natural precipitation and the improvement of soil microbial community structure. Deep plowing was beneficial to the decomposition and metabolism of straw and organic fertilizer, and improved the catabolic ability of microbial community, thus increasing the yield of winter wheat.

Research has revealed that summer fallow sowing improves the water use efficiency (WUE) and grain yield of winter wheat. However, wheat yields differ yearly with crop management. A field experiment over 8 years was established in the Loess Plateau to determine the role of precipitation and soil water storage in wheat yield formation under conservation tillage. The average WUE values were 7.8, 11.0, and 12.6 t·ha⁻¹, while the average evapotranspiration (ET) values were 334.7, 365.5, and 410 mm when the yields were 3.0, 3.0–4.5, and over 4.5 t·ha⁻¹, respectively. Compared to drill sowing (DS), high water consumption during early growth increased the spike number, grain number, and yield. In years of intermediate or low yields, wide-space sowing (WS) and furrow sowing (FS) improved the ET, WUE, spike number, grain number, and yield of wheat compared to (DS) drill sowing. When the wheat yield was 3.0–4.5 t·ha⁻¹, higher soil water intake during jointing, anthesis, and anthesis–maturity increased the tiller number, 1000-grain weight, and yield, related to the use of suitable tillers. Synchronous increases in grain number per spike and 1000-grain weight were observed with increased soil water content at jointing, maturity, and anthesis, as well as consumption of soil water in the latter part during the growing season.