The aim of this study was to explore the phytochemical composition, heavy metals analysis and the antibacterial activity of six medicinal plants i.e., Terminalia chebula Retz (fruits), Aegle marmelos L., (fruits), Curcuma longa L., (rhizomes), Syzygium aromaticum L., (flower buds), Piper nigrum L., (seeds), Cinnamomum cassia L., (barks) and its two remedial recipes (recipe 1 and 2) used against diarrhea obtained from the local herbal practitioners (Hakeems). A preliminary phytochemical screening of the above-mentioned plants extract in methanol, chloroform, n-hexane and distilled water revealed the presence of various constituents such as alkaloids, flavonoids, tannins and saponins by using standard procedures. The quantitative phytochemical studies shows that alkaloids, flavonoid and saponins were in maximum amount in Terminalia chebula. The concentration of Cd, Ni, Pb, Fe, Cr, Cu and Zn were investigated by using an atomic absorption spectrometer. The obtained analysis shows that Cr, Fe and Pb were present in the highest concentration in medicinal plants and their recipes. The antibacterial activities of the crude extract found in the recipes of methanol, chloroform, n-hexane and distilled water were analyzed by using agar well disc diffusion assay and minimum inhibitory concentration (MIC) by broth dilution method against four bacterial strains, namely, E. coli, Salmonella, Shigella and Methicillin-resistant Staphylococcus aureus (MRSA), respectively. The maximum zones of inhibition in methanol, water, chloroform and n-hexane extracts were seen in recipe 2 against Shigella (22.16 ± 0.47 mm), recipe 2 against Shigella (20.33 ± 0.24 mm), recipe 1 against Shigella (20.30 ± 0.29 mm) and recipe 2 against E. coli (30.23 ± 0.12 mm), respectively. Furthermore, the recipe extracts are more active against the tested bacterial strains than the extracts from individual plants. Therefore, it is concluded that the use of herbal plants and their recipes are the major source of drugs in a traditional medicinal system to cure different diseases.

Plants are a rich source of secondary metabolites that have been found to have medicinal properties. The present study was conducted to evaluate the phytochemical screening, antibacterial activities and heavy metal analysis of seven medicinal plants i.e., Nigella sativa (seeds), Trigonella foenum-graecum (seeds), Brassica campestris (seeds), Pistacia integerrima (galls), Linum usitatissimum (seeds), Hyssopus officinalis (flowers), Ephedra vulgaris (dry branches) and its two recipes which are used by hakims (Practitioners of local herbal medicines), against different diseases particularly respiratory tract infections. The obtained results revealed that alkaloids (30%) and flavonoids (41%) were in maximum quantity in P. integerrima (galls) while saponins (10.9%) were in maximum quantity in Recipe 1. The antibacterial activity was determined by the agar well disc diffusion method using methanol, ethanol, chloroform and deionized water extracts. Each plant extract was tested against one Gram-positive (Streptococcus pneumonia) and two Gram-negative (Pseudomonas aeruginosa and Klebsiella pneumonia) bacteria. Maximum zones of inhibition in methanol, ethanol, chloroform and aqueous extract were seen in T. foenum-graecum against S. pneumonia (20.06 ± 0.16 mm), B. campestris against S. pneumonia (22.40 ± 0.24 mm), Recipe 2 against K. pneumonia (20.06 ± 0.16 mm) and N. sativa against S. pneumonia (20.23 ± 0.16 mm), respectively. The concentrations of heavy metals were determined by atomic absorption spectrophotometer and showed the existence of high concentration of Iron (Fe), Lead (Pb) and Chromium (Cr). Thus, it has been found that medicinal plants individually as well as their recipes are potentially active against various diseases particularly respiratory tract infections