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Bacterioplankton community composition has become the center of research attention in recent years. Bacteria associated with toxic cyanobacteria blooms have attracted considerable interest. However, little is known about the environmental factors driving the bacteria community, including the impact of invasive cyanobacteria. Therefore, our aim has been to determine the relationships between heterotrophic bacteria and phytoplankton community composition across 24 Polish lakes with different contributions of cyanobacteria including the invasive species Raphidiopsis raciborskii. This analysis revealed that cyanobacteria were present in 16 lakes, while R. raciborskii occurred in 14 lakes. Our results show that bacteria communities differed between lakes dominated by cyanobacteria and lakes with minor contributions of cyanobacteria but did not differ between lakes with R. raciborskii and other lakes. Physical factors, including water and Secchi depth, were the major drivers of bacteria and phytoplankton community composition. However, in lakes dominated by cyanobacteria, bacterial community composition was also influenced by biotic factors such as the amount of R. raciborskii, chlorophyll-a and total phytoplankton biomass. Thus, our study provides novel evidence on the influence of environmental factors and R. raciborskii on lake bacteria communities.
Mikołaj Kokociński; Dariusz Dziga; Adam Antosiak; Janne Soininen. Are Bacterio- and Phytoplankton Community Compositions Related in Lakes Differing in Their Cyanobacteria Contribution and Physico-Chemical Properties? Genes 2021, 12, 855 .
AMA StyleMikołaj Kokociński, Dariusz Dziga, Adam Antosiak, Janne Soininen. Are Bacterio- and Phytoplankton Community Compositions Related in Lakes Differing in Their Cyanobacteria Contribution and Physico-Chemical Properties? Genes. 2021; 12 (6):855.
Chicago/Turabian StyleMikołaj Kokociński; Dariusz Dziga; Adam Antosiak; Janne Soininen. 2021. "Are Bacterio- and Phytoplankton Community Compositions Related in Lakes Differing in Their Cyanobacteria Contribution and Physico-Chemical Properties?" Genes 12, no. 6: 855.
Background Excess loads of nutrients finding their way into waterbodies can cause rapid and excessive growth of phytoplankton species and lead to the formation of cyanobacterial harmful algal blooms (cyano-HABs). Toxic cyanobacteria produce a broad range of bioactive metabolites, some of which are known as cyanotoxins. These metabolites can negatively impact the ecosystem, and human and animal health, thus their presence needs to be closely monitored and mitigated. This study aimed to monitor St. George Lake (Athalassa National Forest Park, Cyprus) for its water quality characteristics, and initiate a new methodology to control the bloom that occurred in the lake during summer 2019, by comparing hydrogen peroxide treatment with novel metallic peroxide granules as source of hydrogen peroxide. Results Lake monitoring showed that pH, salinity, total dissolved solids and conductivity varied throughout the year, and nutrients concentration was high, indicating a eutrophic lake. The cyanobacteriumMerismopediasp. bloomed in the lake between June and September 2019, comprising up to 99% of the phytoplankton biovolume. The presence of microcystin synthase encoding gene (mcyB, mcyE) was documented, however microcystins were not detected by tandem mass spectroscopy. Treatment with liquid hydrogen peroxide in concentrations 1 to 5 mg L−1had no effect on the phycocyanin fluorescence (Ft) and quantum yield of PSII (Fv/Fm) indicating an ineffective treatment for the denseMerismopediabloom (1 million cells mL−1 ± 20%). Metallic peroxide granules tested for their H2O2releasing capacity in St. George Lake water, showing that CaO2released higher H2O2concentration and therefore have better mitigation efficiency than MgO2granules. Conclusion The present study highlights the importance of monitoring several water parameters to conclude on the different actions to be taken to limit eutrophication in the catchment area. The findings demonstrated that testing for the presence of genes involved in cyanotoxin production may not be sufficient to follow cyanotoxins in the water, therefore it should be accompanied with analytical confirmation. Treatment experiments indicated that slow release of H2O2from peroxide granules may be an alternative to liquid hydrogen peroxide when applied in appropriate doses, but further investigation is needed before it is applied at the field. Graphic Abstract
Eleni Keliri; Christia Paraskeva; Angelos Sofokleous; Assaf Sukenik; Dariusz Dziga; Ekaterina Chernova; Luc Brient; Maria G. Antoniou. Occurrence of a single-species cyanobacterial bloom in a lake in Cyprus: monitoring and treatment with hydrogen peroxide-releasing granules. Environmental Sciences Europe 2021, 33, 1 -14.
AMA StyleEleni Keliri, Christia Paraskeva, Angelos Sofokleous, Assaf Sukenik, Dariusz Dziga, Ekaterina Chernova, Luc Brient, Maria G. Antoniou. Occurrence of a single-species cyanobacterial bloom in a lake in Cyprus: monitoring and treatment with hydrogen peroxide-releasing granules. Environmental Sciences Europe. 2021; 33 (1):1-14.
Chicago/Turabian StyleEleni Keliri; Christia Paraskeva; Angelos Sofokleous; Assaf Sukenik; Dariusz Dziga; Ekaterina Chernova; Luc Brient; Maria G. Antoniou. 2021. "Occurrence of a single-species cyanobacterial bloom in a lake in Cyprus: monitoring and treatment with hydrogen peroxide-releasing granules." Environmental Sciences Europe 33, no. 1: 1-14.
Senescence is the final stage of plant development, affecting individual organs or the whole organism, and it can be induced by several environmental factors, including shading or darkness. Although inevitable, senescence is a complex and tightly regulated process, ensuring optimal remobilization of nutrients and cellular components from senescing organs. Photoreceptors such as phytochromes and cryptochromes are known to participate in the process of senescence, but the involvement of phototropins has not been studied to date. We investigated the role of these blue light photoreceptors in the senescence of individually darkened Arabidopsis thaliana leaves. We compared several physiological and molecular senescence markers in darkened leaves of wild-type plants and phototropin mutants (phot1, phot2, and phot1phot2). In general, all the symptoms of senescence (lower photochemical activity of photosystem II, photosynthetic pigment degradation, down-regulation of photosynthetic genes, and up-regulation of senescence-associated genes) were less pronounced in phot1phot2, as compared to the wild type, and some also in one of the single mutants, indicating delayed senescence. This points to different mechanisms of phototropin operation in the regulation of senescence-associated processes, either with both photoreceptors acting redundantly, or only one of them, phot1, playing a dominant role.
Aleksandra Eckstein; Joanna Grzyb; Paweł Hermanowicz; Piotr Zgłobicki; Justyna Łabuz; Wojciech Strzałka; Dariusz Dziga; Agnieszka Banaś. Arabidopsis Phototropins Participate in the Regulation of Dark-Induced Leaf Senescence. International Journal of Molecular Sciences 2021, 22, 1836 .
AMA StyleAleksandra Eckstein, Joanna Grzyb, Paweł Hermanowicz, Piotr Zgłobicki, Justyna Łabuz, Wojciech Strzałka, Dariusz Dziga, Agnieszka Banaś. Arabidopsis Phototropins Participate in the Regulation of Dark-Induced Leaf Senescence. International Journal of Molecular Sciences. 2021; 22 (4):1836.
Chicago/Turabian StyleAleksandra Eckstein; Joanna Grzyb; Paweł Hermanowicz; Piotr Zgłobicki; Justyna Łabuz; Wojciech Strzałka; Dariusz Dziga; Agnieszka Banaś. 2021. "Arabidopsis Phototropins Participate in the Regulation of Dark-Induced Leaf Senescence." International Journal of Molecular Sciences 22, no. 4: 1836.
In their life cycle, plants are exposed to various unfavorable environmental factors including ultraviolet (UV) radiation emitted by the Sun. UV-A and UV-B, which are partially absorbed by the ozone layer, reach the surface of the Earth causing harmful effects among the others on plant genetic material. The energy of UV light is sufficient to induce mutations in DNA. Some examples of DNA damage induced by UV are pyrimidine dimers, oxidized nucleotides as well as single and double‑strand breaks. When exposed to light, plants can repair major UV‑induced DNA lesions, i.e., pyrimidine dimers using photoreactivation. However, this highly efficient light‑dependent DNA repair system is ineffective in dim light or at night. Moreover, it is helpless when it comes to the repair of DNA lesions other than pyrimidine dimers. In this review, we have focused on how plants cope with deleterious DNA damage that cannot be repaired by photoreactivation. The current understanding of light‑independent mechanisms, classified as dark DNA repair, indispensable for the maintenance of plant genetic material integrity has been presented.
Wojciech Strzałka; Piotr Zgłobicki; Ewa Kowalska; Aneta Bażant; Dariusz Dziga; Agnieszka Katarzyna Banaś. The Dark Side of UV-Induced DNA Lesion Repair. Genes 2020, 11, 1450 .
AMA StyleWojciech Strzałka, Piotr Zgłobicki, Ewa Kowalska, Aneta Bażant, Dariusz Dziga, Agnieszka Katarzyna Banaś. The Dark Side of UV-Induced DNA Lesion Repair. Genes. 2020; 11 (12):1450.
Chicago/Turabian StyleWojciech Strzałka; Piotr Zgłobicki; Ewa Kowalska; Aneta Bażant; Dariusz Dziga; Agnieszka Katarzyna Banaś. 2020. "The Dark Side of UV-Induced DNA Lesion Repair." Genes 11, no. 12: 1450.
Microcystinase (MlrA) was first described in 1996. Since then MlrA peptidase activity has proven to be both the most efficient enzymatic process and the most specific catalyst of all known microcystins detoxification pathways. Furthermore, MlrA and the MlrABC degradation pathway are presently the only enzymatic processes with clear genetic and biochemical descriptions available for microcystins degradation, greatly facilitating modern applied genetics for any relevant technological development. Recently, there has been increasing interest in the potential of sustainable, biologically inspired alternatives to current industrial practice, with note that biological microcystins degradation is the primary detoxification process found in nature. While previous reviews have broadly discussed microbial biodegradation processes, here we present a review focused specifically on MlrA. Following a general overview, we briefly highlight the initial discovery and present understanding of the MlrABC degradation pathway, before discussing the genetic and biochemical aspects of MlrA. We then review the potential biotechnology applications of MlrA in the context of available literature with emphasis on the optimization of MlrA for in situ applications including (i) direct modulation of Mlr activity within naturally existing populations, (ii) bioaugmentation of systems with introduced biodegradative capacity via whole cell biocatalysts, and (iii) bioremediation via direct MlrA application.
Jason Dexter; Alistair J. McCormick; Pengcheng Fu; Dariusz Dziga. Microcystinase – a review of the natural occurrence, heterologous expression, and biotechnological application of MlrA. Water Research 2020, 189, 116646 .
AMA StyleJason Dexter, Alistair J. McCormick, Pengcheng Fu, Dariusz Dziga. Microcystinase – a review of the natural occurrence, heterologous expression, and biotechnological application of MlrA. Water Research. 2020; 189 ():116646.
Chicago/Turabian StyleJason Dexter; Alistair J. McCormick; Pengcheng Fu; Dariusz Dziga. 2020. "Microcystinase – a review of the natural occurrence, heterologous expression, and biotechnological application of MlrA." Water Research 189, no. : 116646.
Although solar light is indispensable for the functioning of plants, this environmental factor may also cause damage to living cells. Apart from the visible range, including wavelengths used in photosynthesis, the ultraviolet (UV) light present in solar irradiation reaches the Earth’s surface. The high energy of UV causes damage to many cellular components, with DNA as one of the targets. Putting together the puzzle-like elements responsible for the repair of UV-induced DNA damage is of special importance in understanding how plants ensure the stability of their genomes between generations. In this review, we have presented the information on DNA damage produced under UV with a special focus on the pyrimidine dimers formed between the neighboring pyrimidines in a DNA strand. These dimers are highly mutagenic and cytotoxic, thus their repair is essential for the maintenance of suitable genetic information. In prokaryotic and eukaryotic cells, with the exception of placental mammals, this is achieved by means of highly efficient photorepair, dependent on blue/UVA light, which is performed by specialized enzymes known as photolyases. Photolyase properties, as well as their structure, specificity and action mechanism, have been briefly discussed in this paper. Additionally, the main gaps in our knowledge on the functioning of light repair in plant organelles, its regulation and its interaction between different DNA repair systems in plants have been highlighted.
Agnieszka Banaś; Piotr Zgłobicki; Ewa Kowalska; Aneta Bażant; Dariusz Dziga; Wojciech Strzałka. All You Need Is Light. Photorepair of UV-Induced Pyrimidine Dimers. Genes 2020, 11, 1304 .
AMA StyleAgnieszka Banaś, Piotr Zgłobicki, Ewa Kowalska, Aneta Bażant, Dariusz Dziga, Wojciech Strzałka. All You Need Is Light. Photorepair of UV-Induced Pyrimidine Dimers. Genes. 2020; 11 (11):1304.
Chicago/Turabian StyleAgnieszka Banaś; Piotr Zgłobicki; Ewa Kowalska; Aneta Bażant; Dariusz Dziga; Wojciech Strzałka. 2020. "All You Need Is Light. Photorepair of UV-Induced Pyrimidine Dimers." Genes 11, no. 11: 1304.
R. raciborskii is known for growing under wide ranges of temperature and light. In temperate regions, however, low temperature and high light may serve as a stressful condition for invading tropical populations. The genetic basis of R. raciborskii’s adaptation to this combination of stresses are unknown. In this study, the growth rate and the expression of genes that may be crucial in the response to the chill/light stress of two R. raciborskii strains (differing in their climatic origin and toxicity) exposed to low temperature and high light were examined. Results showed that AMU-DH-30, a non CYN (cylindrospermopsin) producing strain isolated from the temperate region, exhibited under stress the upregulation of genes involved in the protein translation (rbp1, nusG, hflX), membrane fluidity (desA), photosynthetic activity (ccr2 and ftsH), and the accumulation of compatible solutes (asd). In contrast, a CYN producing Australian strain CS-505 was not able to adapt quickly and to continue growth during stress conditions. Intriguingly, CS-505 and AMU-DH-30 had a similar ability to resume growth when the stress conditions subsided. Moreover, in strain CS-505 the cyrB gene was significantly upregulated under the stress conditions. The presented results shed new light on the possible mechanisms involved in the response of R.raciborskii to chill/light stress.
Adam Antosiak; Nada Tokodi; Robert Maziarz; Mikołaj Kokociński; Agnieszka Brzozowska; Wojciech Strzałka; Agnieszka Katarzyna Banaś; Anusuya Willis; Dariusz Dziga. Different Gene Expression Response of Polish and Australian Raphidiopsis raciborskii Strains to the Chill/Light Stress. Applied Sciences 2020, 10, 5437 .
AMA StyleAdam Antosiak, Nada Tokodi, Robert Maziarz, Mikołaj Kokociński, Agnieszka Brzozowska, Wojciech Strzałka, Agnieszka Katarzyna Banaś, Anusuya Willis, Dariusz Dziga. Different Gene Expression Response of Polish and Australian Raphidiopsis raciborskii Strains to the Chill/Light Stress. Applied Sciences. 2020; 10 (16):5437.
Chicago/Turabian StyleAdam Antosiak; Nada Tokodi; Robert Maziarz; Mikołaj Kokociński; Agnieszka Brzozowska; Wojciech Strzałka; Agnieszka Katarzyna Banaś; Anusuya Willis; Dariusz Dziga. 2020. "Different Gene Expression Response of Polish and Australian Raphidiopsis raciborskii Strains to the Chill/Light Stress." Applied Sciences 10, no. 16: 5437.
Harmful cyanobacteria and their toxic metabolites constitute a big challenge for the production of safe drinking water. Microcystins (MC), chemically stable hepatotoxic heptapeptides, have often been involved in cyanobacterial poisoning incidents. A desirable solution for cyanobacterial management in lakes and ponds would eliminate both excess cyanobacteria and the MC that they potentially produce and release upon lysis. Hydrogen peroxide (H2O2) has recently been advocated as an efficient means of lysing cyanobacteria in lakes and ponds, however H2O2 (at least when used at typical concentrations) cannot degrade MC in environmental waters. Therefore, mesocosm experiments combining the cyanobacteria-lysing effect of H2O2 and the MC-degrading capacity of the enzyme MlrA were set up in the highly eutrophic Lake Ludoš (Serbia). The H2O2 treatment decreased the abundance of the dominant cyanobacterial taxa Limnothrix sp., Aphanizomenon flos-aquae, and Planktothrix agardhii. The intracellular concentration of MC was reduced/eliminated by H2O2, yet the reduction of the extracellular MC could only be accomplished by supplementation with MlrA. However, as H2O2 was found to induce the expression of mcyB and mcyE genes, which are involved in MC biosynthesis, the use of H2O2 as a safe cyanobacteriocide still requires further investigation. In conclusion, the experiments showed that the combined use of H2O2 and MlrA is promising in the elimination of both excess cyanobacteria and their MC in environmental waters.
Dariusz Dziga; Nada Tokodi; Damjana Drobac; Mikołaj Kokociński; Adam Antosiak; Jakub Puchalski; Wojciech Strzałka; Mariusz Madej; Zorica Svirčev; Jussi Meriluoto; Damjana Drobac Backović. The Effect of a Combined Hydrogen Peroxide-MlrA Treatment on the Phytoplankton Community and Microcystin Concentrations in a Mesocosm Experiment in Lake Ludoš. Toxins 2019, 11, 725 .
AMA StyleDariusz Dziga, Nada Tokodi, Damjana Drobac, Mikołaj Kokociński, Adam Antosiak, Jakub Puchalski, Wojciech Strzałka, Mariusz Madej, Zorica Svirčev, Jussi Meriluoto, Damjana Drobac Backović. The Effect of a Combined Hydrogen Peroxide-MlrA Treatment on the Phytoplankton Community and Microcystin Concentrations in a Mesocosm Experiment in Lake Ludoš. Toxins. 2019; 11 (12):725.
Chicago/Turabian StyleDariusz Dziga; Nada Tokodi; Damjana Drobac; Mikołaj Kokociński; Adam Antosiak; Jakub Puchalski; Wojciech Strzałka; Mariusz Madej; Zorica Svirčev; Jussi Meriluoto; Damjana Drobac Backović. 2019. "The Effect of a Combined Hydrogen Peroxide-MlrA Treatment on the Phytoplankton Community and Microcystin Concentrations in a Mesocosm Experiment in Lake Ludoš." Toxins 11, no. 12: 725.
Microcystins produced by several toxic cyanobacterial strains constitute an important problem for public health. Bacterial degradation of these hepatotoxins may play an important role in natural ecosystems, however the nature of the process is very poorly understood. The aim of our study was to investigate the possible interactions between cyanotoxin producers and degraders. Samples collected from 24 water bodies in western Poland were analysed to determine the chemo-physical parameters, phytoplankton content, bacterial community structure and microcystin-biodegradation potency. A redundancy analysis identified a positive correlation between the capacity of a community to degrade microcystin LR (MC-LR) and temperature, pH, chlorophyll a concentration and the abundance of MC-producers. The relative abundance of classes F38, TM7-3 and the order WCHB1-81c (Actinobacteria) was significantly higher in the lakes with MC-biodegradation potency. Some specific bacterial genera belonging to Acidobacteria, Chloroflexi, Gemmatimonadetes, Firmicutes and TM7 were closely correlated with the occurrence of Microcystis spp. Furthermore, the MC biodegradation process was connected with the same bacterial groups. Thus, our approach allowed us to provide a broader picture of some specific relations between microcystin producers and potential microcystin degraders. A more comprehensive analysis of the existing correlations may be helpful in our understanding of natural mechanisms of MC elimination using bacteria such as MC-degraders.
Dariusz Dziga; Mikołaj Kokociński; Jakub Barylski; Grzegorz Nowicki; Anna Maksylewicz; Adam Antosiak; Agnieszka Katarzyna Banaś; Wojciech Strzałka. Correlation between specific groups of heterotrophic bacteria and microcystin biodegradation in freshwater bodies of central Europe. FEMS Microbiology Ecology 2019, 95, 1 .
AMA StyleDariusz Dziga, Mikołaj Kokociński, Jakub Barylski, Grzegorz Nowicki, Anna Maksylewicz, Adam Antosiak, Agnieszka Katarzyna Banaś, Wojciech Strzałka. Correlation between specific groups of heterotrophic bacteria and microcystin biodegradation in freshwater bodies of central Europe. FEMS Microbiology Ecology. 2019; 95 (11):1.
Chicago/Turabian StyleDariusz Dziga; Mikołaj Kokociński; Jakub Barylski; Grzegorz Nowicki; Anna Maksylewicz; Adam Antosiak; Agnieszka Katarzyna Banaś; Wojciech Strzałka. 2019. "Correlation between specific groups of heterotrophic bacteria and microcystin biodegradation in freshwater bodies of central Europe." FEMS Microbiology Ecology 95, no. 11: 1.
In this report, we establish proof-of-principle demonstrating for the first time genetic engineering of a photoautotrophic microorganism for bioremediation of naturally occurring cyanotoxins. In model cyanobacterium Synechocystis sp. PCC 6803 we have heterologously expressed Sphingopyxis sp. USTB-05 microcystinase (MlrA) bearing a 23 amino acid N-terminus secretion peptide from native Synechocystis sp. PCC 6803 PilA (sll1694). The resultant whole cell biocatalyst displayed about 3 times higher activity against microcystin-LR compared to a native MlrA host (Sphingomonas sp. ACM 3962), normalized for optical density. In addition, MlrA activity was found to be almost entirely located in the cyanobacterial cytosolic fraction, despite the presence of the secretion tag, with crude cellular extracts showing MlrA activity comparable to extracts from MlrA expressing E. coli. Furthermore, despite approximately 9.4-fold higher initial MlrA activity of a whole cell E. coli biocatalyst, utilization of a photoautotrophic chassis resulted in prolonged stability of MlrA activity when cultured under semi-natural conditions (using lake water), with the heterologous MlrA biocatalytic activity of the E. coli culture disappearing after 4 days, while the cyanobacterial host displayed activity (3% of initial activity) after 9 days. In addition, the cyanobacterial cell density was maintained over the duration of this experiment while the cell density of the E. coli culture rapidly declined. Lastly, failure to establish a stable cyanobacterial isolate expressing native MlrA (without the N-terminus tag) via the strong cpcB560 promoter draws attention to the use of peptide tags to positively modulate expression of potentially toxic proteins.
Jason Dexter; Dariusz Dziga; Jing Lv; Junqi Zhu; Wojciech Strzalka; Anna Maksylewicz; Magdalena Maroszek; Sylwia Marek; Pengcheng Fu. Heterologous expression of mlrA in a photoautotrophic host – Engineering cyanobacteria to degrade microcystins. Environmental Pollution 2018, 237, 926 -935.
AMA StyleJason Dexter, Dariusz Dziga, Jing Lv, Junqi Zhu, Wojciech Strzalka, Anna Maksylewicz, Magdalena Maroszek, Sylwia Marek, Pengcheng Fu. Heterologous expression of mlrA in a photoautotrophic host – Engineering cyanobacteria to degrade microcystins. Environmental Pollution. 2018; 237 ():926-935.
Chicago/Turabian StyleJason Dexter; Dariusz Dziga; Jing Lv; Junqi Zhu; Wojciech Strzalka; Anna Maksylewicz; Magdalena Maroszek; Sylwia Marek; Pengcheng Fu. 2018. "Heterologous expression of mlrA in a photoautotrophic host – Engineering cyanobacteria to degrade microcystins." Environmental Pollution 237, no. : 926-935.
In some conditions the growth of toxic cyanobacteria must be controlled by treatment with algicidal compounds. Hydrogen peroxide has been proposed as an efficient and relatively safe chemical which can remove cyanobacteria from the environment selectively, without affecting other microorganisms. However, the uncontrolled release of secondary metabolites, including toxins may occur after such a treatment. Our proposal presented in this paper is fast biodegradation of microcystin released after cell lysis induced by hydrogen peroxide. The effectiveness of both Sphingomonas sp. and heterologously expressed MlrA enzyme in the removal of the toxin from Microcystis aeruginosa culture has been investigated. The results indicate that neither Sphingomonas cells nor MlrA are affected by hydrogen peroxide in the concentrations which stop the growth of cyanobacteria. A several-fold microcystin reduction was documented in the presence of these agents with biodegradation ability. Our results provide evidence that such a combined treatment of water reservoirs dominated by microcystin-producing cyanobacteria may be a promising alternative which allows fast elimination of toxins from the environment.
Dariusz Dziga; Anna Maksylewicz; Magdalena Maroszek; Sylwia Marek. Combined treatment of toxic cyanobacteria Microcystis aeruginosa with hydrogen peroxide and microcystin biodegradation agents results in quick toxins elimination. Acta Biochimica Polonica 2018, 65, 133 -140.
AMA StyleDariusz Dziga, Anna Maksylewicz, Magdalena Maroszek, Sylwia Marek. Combined treatment of toxic cyanobacteria Microcystis aeruginosa with hydrogen peroxide and microcystin biodegradation agents results in quick toxins elimination. Acta Biochimica Polonica. 2018; 65 (1):133-140.
Chicago/Turabian StyleDariusz Dziga; Anna Maksylewicz; Magdalena Maroszek; Sylwia Marek. 2018. "Combined treatment of toxic cyanobacteria Microcystis aeruginosa with hydrogen peroxide and microcystin biodegradation agents results in quick toxins elimination." Acta Biochimica Polonica 65, no. 1: 133-140.
Cyanobacterial blooms and cyanotoxins occur in freshwater lakes and reservoirs all over the world. Bacterial degradation of microcystins (MC), hepatotoxins produced by several cyanobacterial species, has also been broadly documented. However, information regarding MC biodegradation in European water bodies is very limited. In this paper, the occurrence and identification of MC biodegradation products was documented for 21 European lakes and reservoirs, many of which have well-documented cyanobacterial bloom histories. Varying cyanobacterial abundance and taxonomical composition were documented and MC producers were found in all the analysed samples. Planktothrix agardhii was the most common cyanobacterial species and it formed mass occurrences in four lakes. MC biodegradation was observed in 86% of the samples (18 out of 21), and four products of dmMC-LR decomposition were detected by HPLC and LC-MS methods. The two main products were cyclic dmMC-LR with modifications in the Arg-Asp-Leu region; additionally one product was recognized as the tetrapeptide Adda-Glu-Mdha-Ala. The composition of the detected products suggested a new biochemical pathway of MC degradation. The results confirmed the hypothesis that microcystin biodegradation is a common phenomenon in central European waters and that it may occur by a mechanism which is different from the one previously reported. Such a finding implies the necessity to develop a more accurate methodology for screening bacteria with MC biodegradation ability. Furthermore, it warrants new basic and applied studies on the characterization and utilization of new MC-degrading strains and biodegradation pathways.
Dariusz Dziga; Anna Maksylewicz; Magdalena Maroszek; Agnieszka Budzyńska; Agnieszka Napiórkowska-Krzebietke; Magdalena Toporowska; Magdalena Grabowska; Anna Kozak; Joanna Rosińska; Jussi Meriluoto. The biodegradation of microcystins in temperate freshwater bodies with previous cyanobacterial history. Ecotoxicology and Environmental Safety 2017, 145, 420 -430.
AMA StyleDariusz Dziga, Anna Maksylewicz, Magdalena Maroszek, Agnieszka Budzyńska, Agnieszka Napiórkowska-Krzebietke, Magdalena Toporowska, Magdalena Grabowska, Anna Kozak, Joanna Rosińska, Jussi Meriluoto. The biodegradation of microcystins in temperate freshwater bodies with previous cyanobacterial history. Ecotoxicology and Environmental Safety. 2017; 145 ():420-430.
Chicago/Turabian StyleDariusz Dziga; Anna Maksylewicz; Magdalena Maroszek; Agnieszka Budzyńska; Agnieszka Napiórkowska-Krzebietke; Magdalena Toporowska; Magdalena Grabowska; Anna Kozak; Joanna Rosińska; Jussi Meriluoto. 2017. "The biodegradation of microcystins in temperate freshwater bodies with previous cyanobacterial history." Ecotoxicology and Environmental Safety 145, no. : 420-430.
Bacterial degradation of toxic microcystins produced by cyanobacteria is a common phenomenon. However, our understanding of the mechanisms of these processes is rudimentary. In this paper several novel discoveries regarding the action of the enzymes of the mlr cluster responsible for microcystin biodegradation are presented using recombinant proteins. In particular, the predicted active sites of the recombinant MlrB and MlrC were analyzed using functional enzymes and their inactive muteins. A new degradation intermediate, a hexapeptide derived from linearized microcystins by MlrC, was discovered. Furthermore, the involvement of MlrA and MlrB in further degradation of the hexapeptides was confirmed and a corrected biochemical pathway of microcystin biodegradation has been proposed.
Dariusz Dziga; Gabriela Zielińska; Benedykt Wladyka; Oliwia Bochenska; Anna Maksylewicz; Wojciech Strzalka; Jussi Meriluoto. Characterization of Enzymatic Activity of MlrB and MlrC Proteins Involved in Bacterial Degradation of Cyanotoxins Microcystins. Toxins 2016, 8, 76 .
AMA StyleDariusz Dziga, Gabriela Zielińska, Benedykt Wladyka, Oliwia Bochenska, Anna Maksylewicz, Wojciech Strzalka, Jussi Meriluoto. Characterization of Enzymatic Activity of MlrB and MlrC Proteins Involved in Bacterial Degradation of Cyanotoxins Microcystins. Toxins. 2016; 8 (3):76.
Chicago/Turabian StyleDariusz Dziga; Gabriela Zielińska; Benedykt Wladyka; Oliwia Bochenska; Anna Maksylewicz; Wojciech Strzalka; Jussi Meriluoto. 2016. "Characterization of Enzymatic Activity of MlrB and MlrC Proteins Involved in Bacterial Degradation of Cyanotoxins Microcystins." Toxins 8, no. 3: 76.
The occurrence of the cyanobacterial toxin cylindrospermopsin (CYN) in freshwater reservoirs is a common phenomenon. However, the biodegradation of this toxin in environmental samples has been observed only occasionally. In this work the biodegradation ability of cylindrospermopsin was investigated based on isolates from lakes with previous cyanotoxin history. Bacterial strains were identified based on the 16S rDNA and rpoD gene comparison. CYN biodegradation was monitored using the HPLC method. The R6 strain identified as Aeromonas sp. was documented as being capable of CYN removal. This biodegradation was dependent on the pH and temperature. Additionally, the stimulation of the growth of the R6 strain in the presence of CYN was indicated. Our discovery supports the hypothesis that (in analogy to the well-known phenomenon of microcystin biodegradation) in lakes dominated by potential CYN-producing cyanobacteria, the processes of microbial utilization of this toxin may occur.
Dariusz Dziga; Mikolaj Kokocinski; Anna Maksylewicz; Urszula Czaja-Prokop; Jakub Barylski. Cylindrospermopsin Biodegradation Abilities of Aeromonas sp. Isolated from Rusałka Lake. Toxins 2016, 8, 55 .
AMA StyleDariusz Dziga, Mikolaj Kokocinski, Anna Maksylewicz, Urszula Czaja-Prokop, Jakub Barylski. Cylindrospermopsin Biodegradation Abilities of Aeromonas sp. Isolated from Rusałka Lake. Toxins. 2016; 8 (3):55.
Chicago/Turabian StyleDariusz Dziga; Mikolaj Kokocinski; Anna Maksylewicz; Urszula Czaja-Prokop; Jakub Barylski. 2016. "Cylindrospermopsin Biodegradation Abilities of Aeromonas sp. Isolated from Rusałka Lake." Toxins 8, no. 3: 55.
Cellulose is a major component of plant biomass and could be applied in the production of biofuels, especially bioethanol. An alternative approach is production of a clean fuel - hydrogen from cellulosic biomass. In this paper an innovatory model of cellulosic waste degradation has been proposed to verify the possibility of utilization of cellulose derivatives by purple non-sulfur bacteria. The concept is based on a two-step process of wheat straw conversion by bacteria in order to obtain an organic acid mixture. In the next stage such products are consumed by Rhodobacter sphaeroides, the known producer of hydrogen. It has been documented that Cellulomonas uda expresses cellulolytic activity in the presence of wheat straw as an only source of carbon. R. sphaeroides applied in this research can effectively consume organic acids released from straw by C. uda and Lactobacillus rhamnosus and is able to grow in the presence of these substrates. Additionally, an increased nitrogenase activity of R. sphaeroides has been indicated when bacteria were cultivated in the presence of cellulose derivatives which suggests that hydrogen production occurs.
Dariusz Dziga; Dominika Jagiełło-Flasińska. Wheat straw degradation and production of alternative substrates for nitrogenase of Rhodobacter sphaeroides. Acta Biochimica Polonica 2015, 62, 395 -400.
AMA StyleDariusz Dziga, Dominika Jagiełło-Flasińska. Wheat straw degradation and production of alternative substrates for nitrogenase of Rhodobacter sphaeroides. Acta Biochimica Polonica. 2015; 62 (3):395-400.
Chicago/Turabian StyleDariusz Dziga; Dominika Jagiełło-Flasińska. 2015. "Wheat straw degradation and production of alternative substrates for nitrogenase of Rhodobacter sphaeroides." Acta Biochimica Polonica 62, no. 3: 395-400.
An important aim of white (grey) biotechnology is bioremediation, where microbes are employed to remove unwanted chemicals. Microcystins (MCs) and other cyanobacterial toxins are not industrial or agricultural pollutants; however, their occurrence as a consequence of human activity and water reservoir eutrophication is regarded as anthropogenic. Microbial degradation of microcystins is suggested as an alternative to chemical and physical methods of their elimination. This paper describes a possible technique of the practical application of the biodegradation process. The idea relies on the utilization of bacteria with a significantly enhanced MC-degradation ability (in comparison with wild strains). The cells of an Escherichia coli laboratory strain expressing microcystinase (MlrA) responsible for the detoxification of MCs were immobilized in alginate beads. The degradation potency of the tested bioreactors was monitored by HPLC detection of linear microcystin LR (MC-LR) as the MlrA degradation product. An open system based on a column filled with alginate-entrapped cells was shown to operate more efficiently than a closed system (alginate beads shaken in a glass container). The maximal degradation rate calculated per one liter of carrier was 219.9 µg h−1 of degraded MC-LR. A comparison of the efficiency of the described system with other biological and chemo-physical proposals suggests that this new idea presents several advantages and is worth investigating in future studies.
Dariusz Dziga; Magdalena Lisznianska; Benedykt Wladyka. Bioreactor Study Employing Bacteria with Enhanced Activity toward Cyanobacterial Toxins Microcystins. Toxins 2014, 6, 2379 -2392.
AMA StyleDariusz Dziga, Magdalena Lisznianska, Benedykt Wladyka. Bioreactor Study Employing Bacteria with Enhanced Activity toward Cyanobacterial Toxins Microcystins. Toxins. 2014; 6 (8):2379-2392.
Chicago/Turabian StyleDariusz Dziga; Magdalena Lisznianska; Benedykt Wladyka. 2014. "Bioreactor Study Employing Bacteria with Enhanced Activity toward Cyanobacterial Toxins Microcystins." Toxins 6, no. 8: 2379-2392.
The occurrence of cyanobacterial toxic peptides, including microcystins (MCs), is an emerging health issue due to the eutrophication of water bodies. MCs have a strong influence on human cells, predominantly hepatocytes, however, toxicity was also observed in kidney, lung and dermal skin cells. Skin as the most external barrier of the human body is responsible for the maintenance of homeostasis of the whole organism. Simultaneously, skin cells may be the most exposed to MCs during recreational activity. The aim of this study was to examine the impact of MC-LR on processes indispensable for normal skin function and regeneration, namely, viability, migration and actin cytoskeleton organization of human keratinocytes. The results showed that short exposure to MC-LR does not affect proliferation of human skin keratinocytes but it is toxic after longer incubation in dose-dependent manner. Total disruption of the actin cytoskeleton was observed under the same MC-LR concentration. Furthermore, keratinocyte migration was inhibited at MC-LR concentrations of 50 μM after incubation for only 4 h. Some of the negative impacts of MC-LR on the examined cell processes may be partly reversible. The observed effects, regarding the possible high exposition of keratinocytes to toxins including MCs, are severe and may cause diverse health problems.
Marcin Kozdęba; Julia Borowczyk; Eliza Zimoląg; Marcin Wasylewski; Dariusz Dziga; Zbigniew Madeja; Justyna Drukala. Microcystin-LR affects properties of human epidermal skin cells crucial for regenerative processes. Toxicon 2014, 80, 38 -46.
AMA StyleMarcin Kozdęba, Julia Borowczyk, Eliza Zimoląg, Marcin Wasylewski, Dariusz Dziga, Zbigniew Madeja, Justyna Drukala. Microcystin-LR affects properties of human epidermal skin cells crucial for regenerative processes. Toxicon. 2014; 80 ():38-46.
Chicago/Turabian StyleMarcin Kozdęba; Julia Borowczyk; Eliza Zimoląg; Marcin Wasylewski; Dariusz Dziga; Zbigniew Madeja; Justyna Drukala. 2014. "Microcystin-LR affects properties of human epidermal skin cells crucial for regenerative processes." Toxicon 80, no. : 38-46.
Hepatotoxic microcystins that are produced by freshwater cyanobacteria pose a risk to public health. These compounds may be eliminated by enzymatic degradation. Here, we review the enzymatic pathways for the degradation of these hepatotoxins, some of which are newly discovered processes. The efficiencies of microcystin biodegradation pathways are documented in several papers and are compared here. Additionally, a comprehensive description of the microcystin enzymatic degradation scheme has been supplemented with a proposal for a new biodegradation pathway. Critical comments on less documented hypotheses are also included. The genetic aspects of biodegradation activity are discussed in detail. We also describe some methods that are useful for studying the biological decomposition of microcystins, including screening for microcystin degraders and detecting microcystin degradation products, with an emphasis on mass spectrometric methodology.
Dariusz Dziga; Marcin Wasylewski; Benedykt Wladyka; Sonja Nybom; Jussi Meriluoto. Microbial Degradation of Microcystins. Chemical Research in Toxicology 2013, 26, 841 -852.
AMA StyleDariusz Dziga, Marcin Wasylewski, Benedykt Wladyka, Sonja Nybom, Jussi Meriluoto. Microbial Degradation of Microcystins. Chemical Research in Toxicology. 2013; 26 (6):841-852.
Chicago/Turabian StyleDariusz Dziga; Marcin Wasylewski; Benedykt Wladyka; Sonja Nybom; Jussi Meriluoto. 2013. "Microbial Degradation of Microcystins." Chemical Research in Toxicology 26, no. 6: 841-852.
Dariusz Dziga; Magdalena Sworzen; Benedykt Wladyka; Marcin Wasylewski. Genetically Engineered Bacteria Immobilized in Alginate as an Option of Cyanotoxins Removal. International Journal of Environmental Science and Development 2013, 360 -364.
AMA StyleDariusz Dziga, Magdalena Sworzen, Benedykt Wladyka, Marcin Wasylewski. Genetically Engineered Bacteria Immobilized in Alginate as an Option of Cyanotoxins Removal. International Journal of Environmental Science and Development. 2013; ():360-364.
Chicago/Turabian StyleDariusz Dziga; Magdalena Sworzen; Benedykt Wladyka; Marcin Wasylewski. 2013. "Genetically Engineered Bacteria Immobilized in Alginate as an Option of Cyanotoxins Removal." International Journal of Environmental Science and Development , no. : 360-364.
The MlrC protein from Sphingomonas ACM-3962 strain was heterologously expressed in Escherichia coli strain BL21(DE3) and purified to investigate participation of this enzyme in the biodegradation of two microcystin variants. In contrast with previous reports, our results indicated that MlrC cleaves linear microcystins, thus shedding new light on the role of MlrC enzyme in microcystin biodegradation.
Dariusz Dziga; Marcin Wasylewski; Adam Szetela; Oliwia Bocheńska; Benedykt Wladyka. Verification of the Role of MlrC in Microcystin Biodegradation by Studies Using a Heterologously Expressed Enzyme. Chemical Research in Toxicology 2012, 25, 1192 -1194.
AMA StyleDariusz Dziga, Marcin Wasylewski, Adam Szetela, Oliwia Bocheńska, Benedykt Wladyka. Verification of the Role of MlrC in Microcystin Biodegradation by Studies Using a Heterologously Expressed Enzyme. Chemical Research in Toxicology. 2012; 25 (6):1192-1194.
Chicago/Turabian StyleDariusz Dziga; Marcin Wasylewski; Adam Szetela; Oliwia Bocheńska; Benedykt Wladyka. 2012. "Verification of the Role of MlrC in Microcystin Biodegradation by Studies Using a Heterologously Expressed Enzyme." Chemical Research in Toxicology 25, no. 6: 1192-1194.