Fusarium head blight (FHB) is a devastating disease of wheat worldwide. In addition to reducing the yield of the crop, the causal pathogens also produce mycotoxins that can contaminate the grain. The development of resistant wheat varieties is one of the best ways to reduce the impact of FHB. To develop such varieties, breeders must expose germplasm lines to the pathogen in the field and assess the disease reaction. Phenotyping breeding materials for resistance to FHB is time-consuming, labor-intensive, and expensive when using conventional protocols. To develop a reliable and cost-effective high throughput phenotyping system for assessing FHB in the field, we focused on developing a method for processing color images of wheat spikes to accurately detect diseased areas using deep learning and image processing techniques. Color images of wheat spikes at the milk stage were collected in a shadow condition and processed to construct datasets, which were used to retrain a deep convolutional neural network model using transfer learning. Testing results showed that the model detected spikes very accurately in the images since the coefficient of determination for the number of spikes tallied by manual count and the model was 0.80. The model was assessed, and the mean average precision for the testing dataset was 0.9201. On the basis of the results for spike detection, a new color feature was applied to obtain the gray image of each spike and a modified region-growing algorithm was implemented to segment and detect the diseased areas of each spike. Results showed that the region growing algorithm performed better than the K-means and Otsu’s method in segmenting diseased areas. We demonstrated that deep learning techniques enable accurate detection of FHB in wheat based on color image analysis, and the proposed method can effectively detect spikes and diseased areas, which improves the efficiency of the FHB assessment in the field.

Sensors for measuring plant phenotyping: A review

Fusarium head blight (FHB) disease is extensively distributed worldwide. This disease damages grain quality and reduces yield. The detection of this disease in a high throughput way is crucial to planters and breeders. Our study focused on developing a method for processing wheat color images and accurately detecting disease areas using deep learning and image processing techniques. The color images of wheat at the milky stage were collected and processed to construct datasets, which were used to retrain a deep convolutional neural network model using transfer learning. Testing results showed that the model can detect spikes, and the coefficient of determination of the number of spikes between the manual count and the detection was 0.80. The model was assessed, and the mean average precision for the testing dataset was 0.9201. On the basis of the results of spike detection, a new color feature was applied to obtain the gray image of each spike. Then, a modified region growing algorithm was implemented to segment and detect the diseased areas of each spike. Results show that the region growing algorithm performs better than K-means and Otsu’s method in segmenting the FHB disease. Overall, this study demonstrates that deep learning techniques enable the accurate detection of FHB in wheat using color images, and the proposed method can effectively detect spikes and diseased areas, thereby improving the efficiency of FHB detection.

Fusarium head blight (FHB), a frequent disease in wheat cultivation, can lead to substantial yield losses and the production of mycotoxins in grains. There