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Xianbao Deng
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China

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Journal article
Published: 08 May 2021 in Postharvest Biology and Technology
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The rapid deteriorative quality is a major factor that currently limits storage and transport of fresh lotus seeds. However, the physiological changes and molecular mechanisms of lotus seeds during postharvest storage remains poorly understood. Here, physiological and RNA-sequencing analyses were conducted on the postharvest seeds of seed-lotus cultivar ‘Jianxuan 17’. A rapid increase in starch and protein content was observed, while soluble sugar content was continuously decreased during postharvest storage, which could explain increased hardness and reduced sweetness of lotus seeds. Transcriptome analysis identified a total of 3148 differentially expressed genes (DEGs), and functional enrichment analysis showed six pathways that included starch and sucrose metabolism were commonly enriched in all comparison groups. Most DEGs involved in energy metabolic pathways, such as glycolysis and tricarboxylic acid cycle were down-regulated. Altered starch and soluble sugar contents were associated with significant changes in activity of enzymes involved in starch and sucrose metabolism. In addition, the content of plant hormones including, auxin (IAA), jasmonoyl-isoleucine (JA-Ile) and salicylic acid (SA), increased in lotus seeds during postharvest storage, and the activation of signaling transduction pathways were demonstrated at transcriptional level. These results provide not only valuable gene expression dataset for investigating molecular mechanism underlying changes during postharvest storage, but also is a useful reference for developing further preservation technology of fresh lotus seeds.

ACS Style

Heng Sun; Yanling Liu; Junyu Ma; Yunmeng Wang; Heyun Song; Juanjuan Li; Xianbao Deng; Dong Yang; Juan Liu; Minghua Zhang; Yaqian Xiong; Mei Yang. Transcriptome analysis provides strategies for postharvest lotus seeds preservation. Postharvest Biology and Technology 2021, 179, 111583 .

AMA Style

Heng Sun, Yanling Liu, Junyu Ma, Yunmeng Wang, Heyun Song, Juanjuan Li, Xianbao Deng, Dong Yang, Juan Liu, Minghua Zhang, Yaqian Xiong, Mei Yang. Transcriptome analysis provides strategies for postharvest lotus seeds preservation. Postharvest Biology and Technology. 2021; 179 ():111583.

Chicago/Turabian Style

Heng Sun; Yanling Liu; Junyu Ma; Yunmeng Wang; Heyun Song; Juanjuan Li; Xianbao Deng; Dong Yang; Juan Liu; Minghua Zhang; Yaqian Xiong; Mei Yang. 2021. "Transcriptome analysis provides strategies for postharvest lotus seeds preservation." Postharvest Biology and Technology 179, no. : 111583.

Journal article
Published: 25 October 2020 in Plants
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The lotus (Nelumbo nucifera) is one of the most popular aquatic plants in Asia, and has emerged as a novel model for studying flower and rhizome development, and primary and secondary metabolite accumulation. Here, we developed a highly efficient callus induction system for the lotus by optimizing a series of key factors that affect callus formation. The highest efficient callus production was induced on immature cotyledon and embryo explants grown on Murashige and Skoog (MS) basal medium containing an optimized combination of 3 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/L 6-benzylaminopurine (6-BA). In addition, lotus callus induction was proven to be influenced by lotus genotypes, light conditions, the developmental stages of explants and the time of explant sampling. Collecting immature cotyledons from seeds of the genotype “Shilihe 1”, at 9 days post pollination, and to culture the explants in darkness, are proposed as the optimum conditions for lotus callus induction. Interestingly, highly efficient callus induction was also observed in explants of immature embryo derived aseptic seedlings; and a small amount of lotus benzylisoquinoline alkaloid (BIA) and obvious expression of BIA biosynthetic genes were detected in lotus callus.

ACS Style

Xianbao Deng; Yaqian Xiong; Jing Li; Dong Yang; Juan Liu; Heng Sun; Heyun Song; Yunmeng Wang; Junyu Ma; Yanling Liu; Mei Yang. The Establishment of an Efficient Callus Induction System for Lotus (Nelumbo nucifera). Plants 2020, 9, 1436 .

AMA Style

Xianbao Deng, Yaqian Xiong, Jing Li, Dong Yang, Juan Liu, Heng Sun, Heyun Song, Yunmeng Wang, Junyu Ma, Yanling Liu, Mei Yang. The Establishment of an Efficient Callus Induction System for Lotus (Nelumbo nucifera). Plants. 2020; 9 (11):1436.

Chicago/Turabian Style

Xianbao Deng; Yaqian Xiong; Jing Li; Dong Yang; Juan Liu; Heng Sun; Heyun Song; Yunmeng Wang; Junyu Ma; Yanling Liu; Mei Yang. 2020. "The Establishment of an Efficient Callus Induction System for Lotus (Nelumbo nucifera)." Plants 9, no. 11: 1436.

Journal article
Published: 06 October 2020 in BMC Plant Biology
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Background Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. Results We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed. Conclusions Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed. These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content.

ACS Style

Heng Sun; Juanjuan Li; Heyun Song; Dong Yang; Xianbao Deng; Juan Liu; Yunmeng Wang; Junyu Ma; Yaqian Xiong; Yanling Liu; Mei Yang. Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed. BMC Plant Biology 2020, 20, 1 -15.

AMA Style

Heng Sun, Juanjuan Li, Heyun Song, Dong Yang, Xianbao Deng, Juan Liu, Yunmeng Wang, Junyu Ma, Yaqian Xiong, Yanling Liu, Mei Yang. Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed. BMC Plant Biology. 2020; 20 (1):1-15.

Chicago/Turabian Style

Heng Sun; Juanjuan Li; Heyun Song; Dong Yang; Xianbao Deng; Juan Liu; Yunmeng Wang; Junyu Ma; Yaqian Xiong; Yanling Liu; Mei Yang. 2020. "Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed." BMC Plant Biology 20, no. 1: 1-15.

Journal article
Published: 10 October 2019 in International Journal of Molecular Sciences
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The WRKY family is one of the largest transcription factor (TF) families in plants and plays central roles in modulating plant stress responses and developmental processes, as well as secondary metabolic regulations. Lotus (Nelumbo nucifera) is an aquatic crop that has significant food, ornamental and pharmacological values. Here, we performed an overview analysis of WRKY TF family members in lotus, and studied their functions in environmental adaptation and regulation of lotus benzylisoquinoline alkaloid (BIA) biosynthesis. A total of 65 WRKY genes were identified in the lotus genome and they were well clustered in a similar pattern with their Arabidopsis homologs in seven groups (designated I, IIa-IIe, and III), although no lotus WRKY was clustered in the group IIIa. Most lotus WRKYs were functionally paired, which was attributed to the recently occurred whole genome duplication in lotus. In addition, lotus WRKYs were regulated dramatically by salicilic acid (SA), jasmonic acid (JA), and submergence treatments, and two lotus WRKYs, NnWRKY40a and NnWRKY40b, were significantly induced by JA and promoted lotus BIA biosynthesis through activating BIA biosynthetic genes. The investigation of WRKY TFs for this basal eudicot reveals new insights into the evolution of the WRKY family, and provides fundamental information for their functional studies and lotus breeding.

ACS Style

Jing Li; Yacen Xiong; Yi Li; Shiqi Ye; Qi Yin; Siqi Gao; Dong Yang; Mei Yang; E. Tapio Palva; Xianbao Deng. Comprehensive Analysis and Functional Studies of WRKY Transcription Factors in Nelumbo nucifera. International Journal of Molecular Sciences 2019, 20, 5006 .

AMA Style

Jing Li, Yacen Xiong, Yi Li, Shiqi Ye, Qi Yin, Siqi Gao, Dong Yang, Mei Yang, E. Tapio Palva, Xianbao Deng. Comprehensive Analysis and Functional Studies of WRKY Transcription Factors in Nelumbo nucifera. International Journal of Molecular Sciences. 2019; 20 (20):5006.

Chicago/Turabian Style

Jing Li; Yacen Xiong; Yi Li; Shiqi Ye; Qi Yin; Siqi Gao; Dong Yang; Mei Yang; E. Tapio Palva; Xianbao Deng. 2019. "Comprehensive Analysis and Functional Studies of WRKY Transcription Factors in Nelumbo nucifera." International Journal of Molecular Sciences 20, no. 20: 5006.