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In the past, cats were considered resistant to influenza. Today, we know that they are susceptible to some influenza A viruses (IAVs) originating in other species. Usually, the outcome is only subclinical infection or a mild fever. However, outbreaks of feline disease caused by canine H3N2 IAV with fever, tachypnoea, sneezing, coughing, dyspnoea and lethargy are occasionally noted in shelters. In one such outbreak, the morbidity rate was 100% and the mortality rate was 40%. Recently, avian H7N2 IAV infection occurred in cats in some shelters in the USA, inducing mostly mild respiratory disease. Furthermore, cats are susceptible to experimental infection with the human H3N2 IAV that caused the pandemic in 1968. Several studies indicated that cats worldwide could be infected by H1N1 IAV during the subsequent human pandemic in 2009. In one shelter, severe cases with fatalities were noted. Finally, the highly pathogenic avian H5N1 IAV can induce a severe, fatal disease in cats, and can spread via cat-to-cat contact. In this review, the Advisory Board on Cat Diseases (ABCD), a scientifically independent board of experts in feline medicine from 11 European countries, summarises current data regarding the aetiology, epidemiology, pathogenesis, clinical picture, diagnostics, and control of feline IAV infections, as well as the zoonotic risks.
Tadeusz Frymus; Sándor Belák; Herman Egberink; Regina Hofmann-Lehmann; Fulvio Marsilio; Diane Addie; Corine Boucraut-Baralon; Katrin Hartmann; Albert Lloret; Hans Lutz; Maria Pennisi; Etienne Thiry; Uwe Truyen; Séverine Tasker; Karin Möstl; Margaret Hosie. Influenza Virus Infections in Cats. Viruses 2021, 13, 1435 .
AMA StyleTadeusz Frymus, Sándor Belák, Herman Egberink, Regina Hofmann-Lehmann, Fulvio Marsilio, Diane Addie, Corine Boucraut-Baralon, Katrin Hartmann, Albert Lloret, Hans Lutz, Maria Pennisi, Etienne Thiry, Uwe Truyen, Séverine Tasker, Karin Möstl, Margaret Hosie. Influenza Virus Infections in Cats. Viruses. 2021; 13 (8):1435.
Chicago/Turabian StyleTadeusz Frymus; Sándor Belák; Herman Egberink; Regina Hofmann-Lehmann; Fulvio Marsilio; Diane Addie; Corine Boucraut-Baralon; Katrin Hartmann; Albert Lloret; Hans Lutz; Maria Pennisi; Etienne Thiry; Uwe Truyen; Séverine Tasker; Karin Möstl; Margaret Hosie. 2021. "Influenza Virus Infections in Cats." Viruses 13, no. 8: 1435.
Viral recombination is a key mechanism in the evolution and diversity of noroviruses. In vivo, synchronous single-cell coinfection by multiple viruses, the ultimate prerequisite to viral recombination, is likely to be a rare event and delayed secondary infections are a more probable occurrence. Here, we determine the effect of a temporal separation of in vitro infections with the two homologous murine norovirus strains MNV-1 WU20 and CW1 on the composition of nascent viral populations. WU20 and CW1 were either synchronously inoculated onto murine macrophage cell monolayers (coinfection) or asynchronously applied (superinfection with varying titres of CW1 at half-hour to 24-h delays). Then, 24 h after initial co-or superinfection, quantification of genomic copy numbers and discriminative screening of plaque picked infectious progeny viruses demonstrated a time-dependent predominance of primary infecting WU20 in the majority of viral progenies. Our results indicate that a time interval from one to two hours onwards between two consecutive norovirus infections allows for the establishment of a barrier that reduces or prevents superinfection.
Louisa Ludwig-Begall; Elisabetta Di Felice; Barbara Toffoli; Chiara Ceci; Barbara Di Martino; Fulvio Marsilio; Axel Mauroy; Etienne Thiry. Analysis of Synchronous and Asynchronous In Vitro Infections with Homologous Murine Norovirus Strains Reveals Time-Dependent Viral Interference Effects. Viruses 2021, 13, 823 .
AMA StyleLouisa Ludwig-Begall, Elisabetta Di Felice, Barbara Toffoli, Chiara Ceci, Barbara Di Martino, Fulvio Marsilio, Axel Mauroy, Etienne Thiry. Analysis of Synchronous and Asynchronous In Vitro Infections with Homologous Murine Norovirus Strains Reveals Time-Dependent Viral Interference Effects. Viruses. 2021; 13 (5):823.
Chicago/Turabian StyleLouisa Ludwig-Begall; Elisabetta Di Felice; Barbara Toffoli; Chiara Ceci; Barbara Di Martino; Fulvio Marsilio; Axel Mauroy; Etienne Thiry. 2021. "Analysis of Synchronous and Asynchronous In Vitro Infections with Homologous Murine Norovirus Strains Reveals Time-Dependent Viral Interference Effects." Viruses 13, no. 5: 823.
COVID-19 is a severe acute respiratory syndrome (SARS) caused by a new coronavirus (CoV), SARS-CoV-2, which is closely related to SARS-CoV that jumped the animal–human species barrier and caused a disease outbreak in 2003. SARS-CoV-2 is a betacoronavirus that was first described in 2019, unrelated to the commonly occurring feline coronavirus (FCoV) that is an alphacoronavirus associated with feline infectious peritonitis (FIP). SARS-CoV-2 is highly contagious and has spread globally within a few months, resulting in the current pandemic. Felids have been shown to be susceptible to SARS-CoV-2 infection. Particularly in the Western world, many people live in very close contact with their pet cats, and natural infections of cats in COVID-19-positive households have been described in several countries. In this review, the European Advisory Board on Cat Diseases (ABCD), a scientifically independent board of experts in feline medicine from 11 European Countries, discusses the current status of SARS-CoV infections in cats. The review examines the host range of SARS-CoV-2 and human-to-animal transmissions, including infections in domestic and non-domestic felids, as well as mink-to-human/-cat transmission. It summarises current data on SARS-CoV-2 prevalence in domestic cats and the results of experimental infections of cats and provides expert opinions on the clinical relevance and prevention of SARS-CoV-2 infection in cats.
Margaret Hosie; Regina Hofmann-Lehmann; Katrin Hartmann; Herman Egberink; Uwe Truyen; Diane Addie; Sándor Belák; Corine Boucraut-Baralon; Tadeusz Frymus; Albert Lloret; Hans Lutz; Fulvio Marsilio; Maria Pennisi; Séverine Tasker; Etienne Thiry; Karin Möstl. Anthropogenic Infection of Cats during the 2020 COVID-19 Pandemic. Viruses 2021, 13, 185 .
AMA StyleMargaret Hosie, Regina Hofmann-Lehmann, Katrin Hartmann, Herman Egberink, Uwe Truyen, Diane Addie, Sándor Belák, Corine Boucraut-Baralon, Tadeusz Frymus, Albert Lloret, Hans Lutz, Fulvio Marsilio, Maria Pennisi, Séverine Tasker, Etienne Thiry, Karin Möstl. Anthropogenic Infection of Cats during the 2020 COVID-19 Pandemic. Viruses. 2021; 13 (2):185.
Chicago/Turabian StyleMargaret Hosie; Regina Hofmann-Lehmann; Katrin Hartmann; Herman Egberink; Uwe Truyen; Diane Addie; Sándor Belák; Corine Boucraut-Baralon; Tadeusz Frymus; Albert Lloret; Hans Lutz; Fulvio Marsilio; Maria Pennisi; Séverine Tasker; Etienne Thiry; Karin Möstl. 2021. "Anthropogenic Infection of Cats during the 2020 COVID-19 Pandemic." Viruses 13, no. 2: 185.
In industrialized countries, increasing autochthonous infections of hepatitis E virus (HEV) are caused by zoonotic transmission of genotypes (Gts) 3 and 4, mainly through consumption of contaminated raw or undercooked pork meat. Although swine and wild boar are recognized as the main reservoir for Gt3 and Gt4, accumulating evidence indicates that other animal species, including domestic and wild ruminants, may harbor HEV. Herein, we screened molecularly and serologically serum and fecal samples from two domestic and four wild ruminant species collected in Valle d’Aosta and Piemonte regions (northwestern Italy. HEV antibodies were found in sheep (21.6%), goats (11.4%), red deer (2.6%), roe deer (3.1%), and in Alpine ibex (6.3%). Molecular screening was performed using different primer sets targeting highly conserved regions of hepeviruses and HEV RNA, although at low viral loads, was detected in four fecal specimens (3.0%, 4/134) collected from two HEV seropositive sheep herds. Taken together, the data obtained document the circulation of HEV in the geographical area assessed both in wild and domestic ruminants, but with the highest seroprevalence in sheep and goats. Consistently with results from other studies conducted in southern Italy, circulation of HEV among small domestic ruminants seems to occur more frequently than expected.
Andrea Palombieri; Serena Robetto; Federica Di Profio; Vittorio Sarchese; Paola Fruci; Maria Cristina Bona; Giuseppe Ru; Riccardo Orusa; Fulvio Marsilio; Vito Martella; Barbara Di Martino. Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern Italy. Animals 2020, 10, 2351 .
AMA StyleAndrea Palombieri, Serena Robetto, Federica Di Profio, Vittorio Sarchese, Paola Fruci, Maria Cristina Bona, Giuseppe Ru, Riccardo Orusa, Fulvio Marsilio, Vito Martella, Barbara Di Martino. Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern Italy. Animals. 2020; 10 (12):2351.
Chicago/Turabian StyleAndrea Palombieri; Serena Robetto; Federica Di Profio; Vittorio Sarchese; Paola Fruci; Maria Cristina Bona; Giuseppe Ru; Riccardo Orusa; Fulvio Marsilio; Vito Martella; Barbara Di Martino. 2020. "Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern Italy." Animals 10, no. 12: 2351.
Camilla Smoglica; Cristina E. Di Francesco; Simone Angelucci; Antonio Antonucci; Marco Innocenti; Fulvio Marsilio. Occurrence of the tetracycline resistance gene tetA(P) in Apennine wolves (Canis lupus italicus) from different human–wildlife interfaces. Journal of Global Antimicrobial Resistance 2020, 23, 184 -185.
AMA StyleCamilla Smoglica, Cristina E. Di Francesco, Simone Angelucci, Antonio Antonucci, Marco Innocenti, Fulvio Marsilio. Occurrence of the tetracycline resistance gene tetA(P) in Apennine wolves (Canis lupus italicus) from different human–wildlife interfaces. Journal of Global Antimicrobial Resistance. 2020; 23 ():184-185.
Chicago/Turabian StyleCamilla Smoglica; Cristina E. Di Francesco; Simone Angelucci; Antonio Antonucci; Marco Innocenti; Fulvio Marsilio. 2020. "Occurrence of the tetracycline resistance gene tetA(P) in Apennine wolves (Canis lupus italicus) from different human–wildlife interfaces." Journal of Global Antimicrobial Resistance 23, no. : 184-185.
Feline leukaemia virus (FeLV) is a retrovirus associated with fatal disease in progressively infected cats. While testing/removal and vaccination led to a decreased prevalence of FeLV, recently, this decrease has reportedly stagnated in some countries. This study aimed to prospectively determine the prevalence of FeLV viraemia in cats taken to veterinary facilities in 32 European countries. FeLV viral RNA was semiquantitatively detected in saliva, using RT-qPCR as a measure of viraemia. Risk and protective factors were assessed using an online questionnaire to report geographic, demographic, husbandry, FeLV vaccination, and clinical data. The overall prevalence of FeLV viraemia in cats visiting a veterinary facility, of which 10.4% were shelter and rescue cats, was 2.3% (141/6005; 95% CI: 2.0%–2.8%) with the highest prevalences in Portugal, Hungary, and Italy/Malta (5.7%–8.8%). Using multivariate analysis, seven risk factors (Southern Europe, male intact, 1–6 years of age, indoor and outdoor or outdoor-only living, living in a group of ≥5 cats, illness), and three protective factors (Northern Europe, Western Europe, pedigree cats) were identified. Using classification and regression tree (CART) analysis, the origin of cats in Europe, pedigree, and access to outdoors were important predictors of FeLV status. FeLV-infected sick cats shed more viral RNA than FeLV-infected healthy cats, and they suffered more frequently from anaemia, anorexia, and gingivitis/stomatitis than uninfected sick cats. Most cats had never been FeLV-vaccinated; vaccination rates were indirectly associated with the gross domestic product (GDP) per capita. In conclusion, we identified countries where FeLV was undetectable, demonstrating that the infection can be eradicated and highlighting those regions where awareness and prevention should be increased.
Nadine Studer; Hans Lutz; Claude Saegerman; Enikö Gönczi; Marina L. Meli; Gianluca Boo; Katrin Hartmann; Margaret J. Hosie; Karin Moestl; Séverine Tasker; Sándor Belák; Albert Lloret; Corine Boucraut-Baralon; Herman F. Egberink; Maria-Grazia Pennisi; Uwe Truyen; Tadeusz Frymus; Etienne Thiry; Fulvio Marsilio; Diane Addie; Manfred Hochleithner; Filip Tkalec; Zsuzsanna Vizi; Anna Brunetti; Boyko Georgiev; Louisa F. Ludwig-Begall; Flurin Tschuor; Carmel T. Mooney; Catarina Eliasson; Janne Orro; Helle Johansen; Kirsi Juuti; Igor Krampl; Kaspars Kovalenko; Jakov Å engaut; Cristina Sobral; Petra Borska; Simona KovaÅ™Ãková; Regina Hofmann-Lehmann. Pan-European Study on the Prevalence of the Feline Leukaemia Virus Infection – Reported by the European Advisory Board on Cat Diseases (ABCD Europe). Viruses 2019, 11, 993 .
AMA StyleNadine Studer, Hans Lutz, Claude Saegerman, Enikö Gönczi, Marina L. Meli, Gianluca Boo, Katrin Hartmann, Margaret J. Hosie, Karin Moestl, Séverine Tasker, Sándor Belák, Albert Lloret, Corine Boucraut-Baralon, Herman F. Egberink, Maria-Grazia Pennisi, Uwe Truyen, Tadeusz Frymus, Etienne Thiry, Fulvio Marsilio, Diane Addie, Manfred Hochleithner, Filip Tkalec, Zsuzsanna Vizi, Anna Brunetti, Boyko Georgiev, Louisa F. Ludwig-Begall, Flurin Tschuor, Carmel T. Mooney, Catarina Eliasson, Janne Orro, Helle Johansen, Kirsi Juuti, Igor Krampl, Kaspars Kovalenko, Jakov Å engaut, Cristina Sobral, Petra Borska, Simona KovaÅ™Ãková, Regina Hofmann-Lehmann. Pan-European Study on the Prevalence of the Feline Leukaemia Virus Infection – Reported by the European Advisory Board on Cat Diseases (ABCD Europe). Viruses. 2019; 11 (11):993.
Chicago/Turabian StyleNadine Studer; Hans Lutz; Claude Saegerman; Enikö Gönczi; Marina L. Meli; Gianluca Boo; Katrin Hartmann; Margaret J. Hosie; Karin Moestl; Séverine Tasker; Sándor Belák; Albert Lloret; Corine Boucraut-Baralon; Herman F. Egberink; Maria-Grazia Pennisi; Uwe Truyen; Tadeusz Frymus; Etienne Thiry; Fulvio Marsilio; Diane Addie; Manfred Hochleithner; Filip Tkalec; Zsuzsanna Vizi; Anna Brunetti; Boyko Georgiev; Louisa F. Ludwig-Begall; Flurin Tschuor; Carmel T. Mooney; Catarina Eliasson; Janne Orro; Helle Johansen; Kirsi Juuti; Igor Krampl; Kaspars Kovalenko; Jakov Å engaut; Cristina Sobral; Petra Borska; Simona KovaÅ™Ãková; Regina Hofmann-Lehmann. 2019. "Pan-European Study on the Prevalence of the Feline Leukaemia Virus Infection – Reported by the European Advisory Board on Cat Diseases (ABCD Europe)." Viruses 11, no. 11: 993.
Recent advances in the diagnostic and metagenomic investigations of the feline enteric environment have allowed the identification of several novel viruses that have been associated with gastroenteritis in cats. In the last few years, noroviruses, kobuviruses, and novel parvoviruses have been repetitively detected in diarrheic cats as alone or in mixed infections with other pathogens, raising a number of questions, with particular regards to their pathogenic attitude and clinical impact. In the present article, the current available literature on novel potential feline enteric viruses is reviewed, providing a meaningful update on the etiology, epidemiologic, pathogenetic, clinical, and diagnostic aspects of the infections caused by these pathogens.
Barbara Di Martino; Federica Di Profio; Irene Melegari; Fulvio Marsilio; Di Martino; Di Profio. Feline Virome—A Review of Novel Enteric Viruses Detected in Cats. Viruses 2019, 11, 908 .
AMA StyleBarbara Di Martino, Federica Di Profio, Irene Melegari, Fulvio Marsilio, Di Martino, Di Profio. Feline Virome—A Review of Novel Enteric Viruses Detected in Cats. Viruses. 2019; 11 (10):908.
Chicago/Turabian StyleBarbara Di Martino; Federica Di Profio; Irene Melegari; Fulvio Marsilio; Di Martino; Di Profio. 2019. "Feline Virome—A Review of Novel Enteric Viruses Detected in Cats." Viruses 11, no. 10: 908.
Fulvio Marsilio. Vaccination against canine parvoviral enteritis in healthy dogs. Veterinary Record 2015, 177, 595 -596.
AMA StyleFulvio Marsilio. Vaccination against canine parvoviral enteritis in healthy dogs. Veterinary Record. 2015; 177 (23):595-596.
Chicago/Turabian StyleFulvio Marsilio. 2015. "Vaccination against canine parvoviral enteritis in healthy dogs." Veterinary Record 177, no. 23: 595-596.
Feline calicivirus (FCV) is considered the most common upper respiratory tract disease (URTD) associated pathogen in cats. We previously expressed FCV VP1 capsid protein in insect cells by baculovirus system and we observed that this protein self-assemble into virus-like particles (VLPs) different in size and lacking the typical cup-like depressions of caliciviruses. In the present study, VP1 and the small basic structural protein VP2 of FCV were individually expressed by baculovirus system. Coinfection of insect cells with both recombinant viruses resulted in VP1 and VP2 self-assembly to form depressions similar to native capsids in size and appearance, demonstrating that VP2 interacts with the VP1 protein in the formation of VLPs.
B. Di Martino; F. Marsilio. Feline calicivirus VP2 is involved in the self-assembly of the capsid protein into virus-like particles. Research in Veterinary Science 2010, 89, 279 -281.
AMA StyleB. Di Martino, F. Marsilio. Feline calicivirus VP2 is involved in the self-assembly of the capsid protein into virus-like particles. Research in Veterinary Science. 2010; 89 (2):279-281.
Chicago/Turabian StyleB. Di Martino; F. Marsilio. 2010. "Feline calicivirus VP2 is involved in the self-assembly of the capsid protein into virus-like particles." Research in Veterinary Science 89, no. 2: 279-281.
This article reports the results of necropsy, parasitologic, microbiologic, histopathologic, immunohistochemical, indirect immunofluorescence, biomolecular, and serologic investigations on 8 striped dolphins ( Stenella coeruleoalba) found stranded from August to December 2007 on the Ligurian Sea coast of Italy. Severe, nonsuppurative meningoencephalitis was found in 4 animals, as characterized by prominent perivascular mononuclear cell cuffing and macrophage accumulations in neuropil. These lesions were associated with mild lymphocytic–plasmacytic infiltration of choroid plexuses in 1 dolphin. Toxoplasma gondii cysts and zoites, confirmed by immunohistochemical labeling, were scattered throughout the brain parenchyma of 2 of the 4 dolphins. No viral inclusions were seen in the brain of any animal. Other findings included severe bronchointerstitial pneumonia and pulmonary atelectasis, consolidation, and emphysema. Parasites were identified in a variety of organs, including lung ( Halocerchus lagenorhynchi). Microbiologic and serologic examinations for Brucella spp were negative on all 8 dolphins. The 4 animals with meningoencephalitis had serum antibodies against T gondii (titers ranging from 1:80 to 1:320) but not against morbillivirus. In contrast, the other 4 dolphins were seropositive for morbillivirus (with titers ranging from 1:10 to 1:40) but seronegative for T gondii. No morbillivirus antigen or nucleic acid was detected in the tissues of any dolphin. It is concluded that the severe lung and brain lesions were the cause of death and that T gondii was the likely etiologic agent of the cerebral lesions. Morbillivirus infection was not considered to have contributed to death of these animals.
G. Di Guardo; U. Proietto; C. E. Di Francesco; F. Marsilio; A. Zaccaroni; D. Scaravelli; W. Mignone; F. Garibaldi; S. Kennedy; F. Forster; B. Iulini; E. Bozzetta; Cristina Casalone. Cerebral Toxoplasmosis in Striped Dolphins (Stenella coeruleoalba) Stranded Along the Ligurian Sea Coast of Italy. Veterinary Pathology 2009, 47, 245 -253.
AMA StyleG. Di Guardo, U. Proietto, C. E. Di Francesco, F. Marsilio, A. Zaccaroni, D. Scaravelli, W. Mignone, F. Garibaldi, S. Kennedy, F. Forster, B. Iulini, E. Bozzetta, Cristina Casalone. Cerebral Toxoplasmosis in Striped Dolphins (Stenella coeruleoalba) Stranded Along the Ligurian Sea Coast of Italy. Veterinary Pathology. 2009; 47 (2):245-253.
Chicago/Turabian StyleG. Di Guardo; U. Proietto; C. E. Di Francesco; F. Marsilio; A. Zaccaroni; D. Scaravelli; W. Mignone; F. Garibaldi; S. Kennedy; F. Forster; B. Iulini; E. Bozzetta; Cristina Casalone. 2009. "Cerebral Toxoplasmosis in Striped Dolphins (Stenella coeruleoalba) Stranded Along the Ligurian Sea Coast of Italy." Veterinary Pathology 47, no. 2: 245-253.
B Di Martino; C E Di Francesco; I Meridiani; F Marsilio. Etiological investigation of multiple respiratory infections in cats. The new microbiologica 2007, 30, 1 .
AMA StyleB Di Martino, C E Di Francesco, I Meridiani, F Marsilio. Etiological investigation of multiple respiratory infections in cats. The new microbiologica. 2007; 30 (4):1.
Chicago/Turabian StyleB Di Martino; C E Di Francesco; I Meridiani; F Marsilio. 2007. "Etiological investigation of multiple respiratory infections in cats." The new microbiologica 30, no. 4: 1.
Fourteen blood samples collected from dogs that were seropositive for Ehrlichia canis were examined for the presence of the citrate synthase gene using a highly specific and sensitive novel polymerase chain reaction assay. The assay detected E. canis DNA in 3 dogs. The complete nucleotide sequence of the citrate synthase gene was determined in 2 of the test-positive samples, and represents the first sequence of the gene to be derived from Italian isolates. The sequence data displayed high identity (99.2%) between the geographically separated Italian samples and the Oklahoma strain of E. canis. The high-sequence conservation revealed by molecular analysis confirmed the usefulness of the citrate synthase gene as a target for detection of E. canis.
Fulvio Marsilio; Barbara Di Martino; Ilaria Meridiani; Paolo Bianciardi. Direct Identification of Ehrlichia Canis by a Novel Polymerase Chain Reaction Method and Molecular Analysis of the Citrate Synthase (gltA) Gene from Various Italian Strains. Journal of Veterinary Diagnostic Investigation 2006, 18, 215 -217.
AMA StyleFulvio Marsilio, Barbara Di Martino, Ilaria Meridiani, Paolo Bianciardi. Direct Identification of Ehrlichia Canis by a Novel Polymerase Chain Reaction Method and Molecular Analysis of the Citrate Synthase (gltA) Gene from Various Italian Strains. Journal of Veterinary Diagnostic Investigation. 2006; 18 (2):215-217.
Chicago/Turabian StyleFulvio Marsilio; Barbara Di Martino; Ilaria Meridiani; Paolo Bianciardi. 2006. "Direct Identification of Ehrlichia Canis by a Novel Polymerase Chain Reaction Method and Molecular Analysis of the Citrate Synthase (gltA) Gene from Various Italian Strains." Journal of Veterinary Diagnostic Investigation 18, no. 2: 215-217.
F Marsilio; B Di Martino; C E Di Francesco; I Meridiani. Diagnosis of ovine chlamydial abortions by PCR-RFLP performed on vaginal swabs. Veterinary Research Communications 2005, 29, 1 .
AMA StyleF Marsilio, B Di Martino, C E Di Francesco, I Meridiani. Diagnosis of ovine chlamydial abortions by PCR-RFLP performed on vaginal swabs. Veterinary Research Communications. 2005; 29 ():1.
Chicago/Turabian StyleF Marsilio; B Di Martino; C E Di Francesco; I Meridiani. 2005. "Diagnosis of ovine chlamydial abortions by PCR-RFLP performed on vaginal swabs." Veterinary Research Communications 29, no. : 1.
Ovine enzootic abortion is an infectious and contagious disease clinically characterized by abortion and weak neonates, affecting sheep and goats. The etiological agent is Chlamydophila (C.) abortus, which is considered one of the most common animal pathogens of small ruminants; it has important economic implications and represents a significant zoonotic risk. Clinical diagnosis is often difficult because the clinical signs and the pathological lesions are not specific for C. abortus infection, in fact they can also be observed as a result of infections with other abortifacient agents. Moreover, the involvement of the laboratory is necessary to perform the definitive diagnosis. One hundred and seventeen vaginal swabs from sheep with clinical signs related to chlamydial infection were examined by a PCR-RFLP assay that demonstrated high specifity and sensitivity. Six samples were positive for C. abortus. Vaginal swabs are easy to handle and allow to deal with biohazardous material in safety conditions.
F. Marsilio; B. Martino; C. E. Francesco; I. Meridiani. Diagnosis of ovine chlamydial abortions by PCR-RFLP performed on vaginal swabs. Veterinary Research Communications 2005, 29, 99 -106.
AMA StyleF. Marsilio, B. Martino, C. E. Francesco, I. Meridiani. Diagnosis of ovine chlamydial abortions by PCR-RFLP performed on vaginal swabs. Veterinary Research Communications. 2005; 29 (1):99-106.
Chicago/Turabian StyleF. Marsilio; B. Martino; C. E. Francesco; I. Meridiani. 2005. "Diagnosis of ovine chlamydial abortions by PCR-RFLP performed on vaginal swabs." Veterinary Research Communications 29, no. 1: 99-106.
A novel nested PCR (nPCR) assay is reported on the diagnosis of the feline calicivirus (FCV) infection. The test was performed on 47 ocular and 40 pharyngeal swabs collected from 47 cats with respiratory syndrome; among the 87 samples examined, 18 ocular and 23 pharyngeal swabs were positive in nPCR. The nPCR sensitivity was compared to other diagnostic techniques such as virus isolation on cell culture and reverse transcriptase-polymerase chain reaction (RT-PCR). The nPCR was more sensitive than the virus isolation and RT-PCR; therefore, it can be used for calicivirosis diagnosis in cats.
Fulvio Marsilio; Barbara Di Martino; Nicola Decaro; Canio Buonavoglia. A novel nested PCR for the diagnosis of calicivirus infections in the cat. Veterinary Microbiology 2005, 105, 1 -7.
AMA StyleFulvio Marsilio, Barbara Di Martino, Nicola Decaro, Canio Buonavoglia. A novel nested PCR for the diagnosis of calicivirus infections in the cat. Veterinary Microbiology. 2005; 105 (1):1-7.
Chicago/Turabian StyleFulvio Marsilio; Barbara Di Martino; Nicola Decaro; Canio Buonavoglia. 2005. "A novel nested PCR for the diagnosis of calicivirus infections in the cat." Veterinary Microbiology 105, no. 1: 1-7.
F Marsilio; B Di Martino; C Di Francesco. Use of a duplex-PCR assay to screen for Feline Herpesvirus-1 and Chlamydophila spp. in mucosal swabs from cats. The new microbiologica 2004, 27, 1 .
AMA StyleF Marsilio, B Di Martino, C Di Francesco. Use of a duplex-PCR assay to screen for Feline Herpesvirus-1 and Chlamydophila spp. in mucosal swabs from cats. The new microbiologica. 2004; 27 (3):1.
Chicago/Turabian StyleF Marsilio; B Di Martino; C Di Francesco. 2004. "Use of a duplex-PCR assay to screen for Feline Herpesvirus-1 and Chlamydophila spp. in mucosal swabs from cats." The new microbiologica 27, no. 3: 1.
A Pratelli; G Elia; V Martella; Antonella Tinelli; N DeCaro; F Marsilio; D Buonavoglia; M Tempesta; C Buonavoglia. M gene evolution of canine coronavirus in naturally infected dogs. Veterinary Record 2003, 151, 1 .
AMA StyleA Pratelli, G Elia, V Martella, Antonella Tinelli, N DeCaro, F Marsilio, D Buonavoglia, M Tempesta, C Buonavoglia. M gene evolution of canine coronavirus in naturally infected dogs. Veterinary Record. 2003; 151 (25):1.
Chicago/Turabian StyleA Pratelli; G Elia; V Martella; Antonella Tinelli; N DeCaro; F Marsilio; D Buonavoglia; M Tempesta; C Buonavoglia. 2003. "M gene evolution of canine coronavirus in naturally infected dogs." Veterinary Record 151, no. 25: 1.
C Buonavoglia; F Marsilio; Maria Tempesta; Domenico Buonavoglia; A Cavalli. Persistent pestivirus infection in sheep in Apulia (southern Italy). The new microbiologica 1994, 17, 1 .
AMA StyleC Buonavoglia, F Marsilio, Maria Tempesta, Domenico Buonavoglia, A Cavalli. Persistent pestivirus infection in sheep in Apulia (southern Italy). The new microbiologica. 1994; 17 (2):1.
Chicago/Turabian StyleC Buonavoglia; F Marsilio; Maria Tempesta; Domenico Buonavoglia; A Cavalli. 1994. "Persistent pestivirus infection in sheep in Apulia (southern Italy)." The new microbiologica 17, no. 2: 1.
C Buonavoglia; L Di Trani; Domenico Buonavoglia; Maria Tempesta; F Marsilio. Characterization of Newcastle disease viruses isolated from pigeons in Italy. Microbiologica 1991, 14, 1 .
AMA StyleC Buonavoglia, L Di Trani, Domenico Buonavoglia, Maria Tempesta, F Marsilio. Characterization of Newcastle disease viruses isolated from pigeons in Italy. Microbiologica. 1991; 14 (3):1.
Chicago/Turabian StyleC Buonavoglia; L Di Trani; Domenico Buonavoglia; Maria Tempesta; F Marsilio. 1991. "Characterization of Newcastle disease viruses isolated from pigeons in Italy." Microbiologica 14, no. 3: 1.