Cladosporium species are active in protecting plants against different biotic and abiotic stresses. Since these species produced a wide range of secondary metabolites responsible for the adaptation to new habitats, plant health and performance, they are of great interest, especially for biostimulants in agriculture. Cladosporium sp. produces protein hydrolysates (PHs), a class of biostimulants, by cultivation on medium with keratin wastes (feathers) as carbon and energy sources. The aim of this study was to select a Cladosporium isolate with potential to be used as plant growth promoting agent. The characteristics of Cladosporium isolates as plants biostimulants were evaluated through several tests, such as: antagonism versus plants pathogens, effect on plant growth of secreted volatiles produced by isolates, secretion of hydrolytic enzymes, production of 3-indole acetic acid, zinc and phosphorous solubilization, capacity to promote tomato seedlings growth (pot experiments). Cladosporium isolate T2 presented positive results to all tests. Encouraging results were obtained treating tomato seedlings with PHs from isolate Cladosporium T2 cultured on medium supplemented with 1% (w/w) chicken feathers, for which growth parameters, such as stem weight, stem height, and root weight were significantly higher by 65%, 32%, and 55%, respectively, compared to those treated with water.

Background: The hydrolysis of keratin wastes by microorganisms is considered a biotechnological alternative for recycling and valorization through keratinolytic microorganisms. Despite their resistant structure, keratin wastes can be efficiently degraded by various microorganisms through the secretion of keratinases, which are promising enzymes for several applications, including detergents, fertilizers, and leather and textile industry. In an attempt to isolate keratinolytic microorganisms that can reach commercial exploitation as keratinase producers, the current work assesses the dynamics of keratin biodegradation by several keratinolytic fungal strains isolated from soil. The activity of fungal strains to degrade keratin substrates was evaluated by SEM, FTRIR-ATR spectra and TGA analysis. Results: SEM observations offered relevant information on interactions between microorganism and structural elements of hair strands. FTIR spectra of the bands at 1035–1075 cm-1 assigned to sulfoxide bond appeared because of S–S bond breaking, which demonstrated the initiation of keratin biodegradation. According to TGA, in the second zone of thermal denaturation, where keratin degradation occurs, the highest weight loss of 71.10% was obtained for sample incubated with Fusarium sp. 1A. Conclusions: Among the tested strains, Fusarium sp. 1A was the most active organism in the degradation process with the strongest denaturation of polypeptide chains. Because keratinolytic microorganisms and their enzymes keratinases represent a subject of scientific and economic interest because of their capability to hydrolyze keratin, Fusarium sp. 1A was selected for further studies

This paper presents an overview of the principal structural and dynamics characteristics of reverse micelles (RMs) in order to highlight their structural flexibility and versatility, along with the possibility to modulate their parameters in a controlled manner. The multifunctionality in a large range of different scientific fields is exemplified in two distinct directions: a theoretical model for mimicry of the biological microenvironment and practical application in the field of nanotechnology and nano-based sensors. RMs represent a convenient experimental approach that limits the drawbacks of the conventionally biological studies in vitro, while the particular structure confers them the status of simplified mimics of cells by reproducing a complex supramolecular organization in an artificial system. The biological relevance of RMs is discussed in some particular cases referring to confinement and a crowded environment, as well as the molecular dynamics of water and a cell membrane structure. The use of RMs in a range of applications seems to be more promising due to their structural and compositional flexibility, high efficiency, and selectivity. Advances in nanotechnology are based on developing new methods of nanomaterial synthesis and deposition. This review highlights the advantages of using RMs in the synthesis of nanoparticles with specific properties and in nano (bio)sensor design.

Environmental contamination, extensive exploitation of fuel sources and accessibility of natural renewable resources represent the premises for the development of composite biomaterials. These materials have controlled properties, being obtained through processes operated in mild conditions with low costs, and contributing to the valorization of byproducts from agriculture and industry fields. A novel board composite including lignocelullosic substrate as wheat straws, fungal mycelium and polypropylene embedded with bacterial spores was developed and investigated in the present study. The bacterial spores embedded in polymer were found to be viable even after heat exposure, helping to increase the compatibility of polymer with hydrophilic microorganisms. Fungal based biopolymer composite was obtained after cultivation of Ganoderma lucidum macromycetes on a mixture including wheat straws and polypropylene embedded with spores from Bacillus amyloliquefaciens. Scanning electron microscopy (SEM) and light microscopy images showed the fungal mycelium covering the substrates with a dense network of filaments. The resulted biomaterial is safe, inert, renewable, natural, biodegradable and it can be molded in the desired shape. The fungal biocomposite presented similar compressive strength and improved thermal insulation capacity compared to polystyrene with high potential to be used as thermal insulation material for applications in construction sector.

Decades of technological advancements have led to major environmental concerns, particularly the bioaccumulation of heavy metals, which pose persistent risks to ecosystems and human health. Consequently, research has increasingly shifted from conventional remediation techniques toward more sustainable, environmentally friendly solutions. This review explores recent advancements, ongoing challenges, and future perspectives in the field of bioremediation, emphasizing its potential as a green technology for heavy metal decontamination. Despite significant progress, key challenges remain, including scalability limitations and the management of bioremediation by-products, along with the influence of regulatory policies and public perception on its large-scale implementation. Emerging approaches such as genetic engineering and nanotechnology show promise in overcoming these limitations. Gene editing allows the tailoring of specific metabolic traits for bioprocesses targeted towards increased tolerance to pollutants and higher biodegradation efficiency, higher enzymatic specificity and affinity, and improved yield and fitness in plants. Nanotechnologies, particularly biogenic nanostructures, open up the possibility of repurposing waste materials as well as harnessing the advantages of the biosynthesis of NPs with higher stability, biocompatibility, and biostimulant capacities. Furthermore, biopolymers and bio-based nanocomposites can improve the efficiency and costs of bioremediation protocols. Even so, further research is essential to evaluate their long-term risks and feasibility.

Endometrial cancer is one the most prevalent gynecological cancers and, unfortunately, has a poor prognosis due to low response rates to traditional treatments. However, the progress in molecular biology and understanding the genetic mechanisms involved in tumor processes offers valuable information that has led to the current classification that describes four molecular subtypes of endometrial cancer. This review focuses on the molecular mechanisms involved in the pathogenesis of endometrial cancers, such as genetic mutations, defects in the DNA mismatch repair pathway, epigenetic changes, or dysregulation in angiogenic or hormonal signaling pathways. The preclinical genomic and molecular investigations presented allowed for the identification of some molecules that could be used as biomarkers to diagnose, predict, and monitor the progression of endometrial cancer. Besides the therapies known in clinical practice, targeted therapy is described as a new cancer treatment that involves identifying specific molecular targets in tumor cells. By selectively inhibiting these targets, key signaling pathways involved in cancer progression can be disrupted while normal cells are protected. The connection between molecular biomarkers and targeted therapy is vital in the fight against cancer. Ongoing research and clinical trials are exploring the use of standard therapy agents in combination with other treatment strategies like immunotherapy and anti-angiogenesis therapy to improve outcomes and personalize treatment for patients with endometrial cancer. This approach has the potential to transform the management of cancer patients. In conclusion, enhancing molecular tools is essential for stratifying the risk and guiding surgery, adjuvant therapy, and cancer treatment for women with endometrial cancer. In addition, the information from this review may have an essential value in the personalized therapy approach for endometrial cancer to improve the patient’s life.