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Information on hepatitis E virus (HEV) epidemiology in animal reservoirs in Bulgaria is still lacking. Herein, by screening HEV seropositive sera obtained from Bulgarian swine and wild boars, viral RNA was detected at high prevalence rate (28.2%) in industrial pigs. Sequence analysis of the partial polymerase (RdRp) region revealed the highest genetic correlation with HEVs of genotype (Gt) 3 identified in French and Dutch patients. For three such strains a 700-bp fragment of the ORF 2 gene was generated. On phylogenetic analysis, the Bulgarian strains clustered tightly (93.8-98.3% nt) with human and animal HEVs classified within the Gt3 subtype c.
Andrea Palombieri; Ilia Tsachev; Vittorio Sarchese; Paola Fruci; Federica Di Profio; Roman Pepovich; Magdalena Baymakova; Fulvio Marsilio; Vito Martella; Barbara Di Martino. A Pilot Study on Hepatitis E Virus (HEV) Epidemiology in Animal Reservoirs in Bulgaria. 2021, 1 .
AMA StyleAndrea Palombieri, Ilia Tsachev, Vittorio Sarchese, Paola Fruci, Federica Di Profio, Roman Pepovich, Magdalena Baymakova, Fulvio Marsilio, Vito Martella, Barbara Di Martino. A Pilot Study on Hepatitis E Virus (HEV) Epidemiology in Animal Reservoirs in Bulgaria. . 2021; ():1.
Chicago/Turabian StyleAndrea Palombieri; Ilia Tsachev; Vittorio Sarchese; Paola Fruci; Federica Di Profio; Roman Pepovich; Magdalena Baymakova; Fulvio Marsilio; Vito Martella; Barbara Di Martino. 2021. "A Pilot Study on Hepatitis E Virus (HEV) Epidemiology in Animal Reservoirs in Bulgaria." , no. : 1.
Since the first identification in 1989 in humans, kobuviruses (KoVs) have been identified from a wide range of animal species including carnivores, rodents, birds, ungulates, rabbits, and bats. Several studies have described the identification of genetically related KoVs in the fecal virome of domestic and wild animals suggesting a mutual exchange of viruses. By screening a total of 231 fecal samples from wild and domestic ungulates, KoVs RNA was detected in wild boars (3.2%; 2/63), chamois (4.6%; 2/43), and goats (2.6%; 2/77). On phylogenetic analysis of the partial RdRp sequence, the wild boar strains clustered within the species Aichivirus C whilst the strains identified in domestic and wild ruminants grouped into the species Aichivirus B. The complete VP1 gene was obtained for chamois and goat KoVs. Interestingly, upon phylogenetic analysis the strains grouped together with a KoV of ovine origin within a distinct genetic type (B3) of the species Aichivirus B.
Barbara Di Martino; Federica Di Profio; Serena Robetto; Paola Fruci; Vittorio Sarchese; Andrea Palombieri; Irene Melegari; Riccardo Orusa; Vito Martella; Fulvio Marsilio. Molecular Survey on Kobuviruses in Domestic and Wild Ungulates From Northwestern Italian Alps. Frontiers in Veterinary Science 2021, 8, 1 .
AMA StyleBarbara Di Martino, Federica Di Profio, Serena Robetto, Paola Fruci, Vittorio Sarchese, Andrea Palombieri, Irene Melegari, Riccardo Orusa, Vito Martella, Fulvio Marsilio. Molecular Survey on Kobuviruses in Domestic and Wild Ungulates From Northwestern Italian Alps. Frontiers in Veterinary Science. 2021; 8 ():1.
Chicago/Turabian StyleBarbara Di Martino; Federica Di Profio; Serena Robetto; Paola Fruci; Vittorio Sarchese; Andrea Palombieri; Irene Melegari; Riccardo Orusa; Vito Martella; Fulvio Marsilio. 2021. "Molecular Survey on Kobuviruses in Domestic and Wild Ungulates From Northwestern Italian Alps." Frontiers in Veterinary Science 8, no. : 1.
Viral recombination is a key mechanism in the evolution and diversity of noroviruses. In vivo, synchronous single-cell coinfection by multiple viruses, the ultimate prerequisite to viral recombination, is likely to be a rare event and delayed secondary infections are a more probable occurrence. Here, we determine the effect of a temporal separation of in vitro infections with the two homologous murine norovirus strains MNV-1 WU20 and CW1 on the composition of nascent viral populations. WU20 and CW1 were either synchronously inoculated onto murine macrophage cell monolayers (coinfection) or asynchronously applied (superinfection with varying titres of CW1 at half-hour to 24-h delays). Then, 24 h after initial co-or superinfection, quantification of genomic copy numbers and discriminative screening of plaque picked infectious progeny viruses demonstrated a time-dependent predominance of primary infecting WU20 in the majority of viral progenies. Our results indicate that a time interval from one to two hours onwards between two consecutive norovirus infections allows for the establishment of a barrier that reduces or prevents superinfection.
Louisa Ludwig-Begall; Elisabetta Di Felice; Barbara Toffoli; Chiara Ceci; Barbara Di Martino; Fulvio Marsilio; Axel Mauroy; Etienne Thiry. Analysis of Synchronous and Asynchronous In Vitro Infections with Homologous Murine Norovirus Strains Reveals Time-Dependent Viral Interference Effects. Viruses 2021, 13, 823 .
AMA StyleLouisa Ludwig-Begall, Elisabetta Di Felice, Barbara Toffoli, Chiara Ceci, Barbara Di Martino, Fulvio Marsilio, Axel Mauroy, Etienne Thiry. Analysis of Synchronous and Asynchronous In Vitro Infections with Homologous Murine Norovirus Strains Reveals Time-Dependent Viral Interference Effects. Viruses. 2021; 13 (5):823.
Chicago/Turabian StyleLouisa Ludwig-Begall; Elisabetta Di Felice; Barbara Toffoli; Chiara Ceci; Barbara Di Martino; Fulvio Marsilio; Axel Mauroy; Etienne Thiry. 2021. "Analysis of Synchronous and Asynchronous In Vitro Infections with Homologous Murine Norovirus Strains Reveals Time-Dependent Viral Interference Effects." Viruses 13, no. 5: 823.
Feline chaphamaparvovirus (FeChPV) is a novel parvovirus, first discovered in a multi‐facility feline shelter in Canada in 2019, during an outbreak of acute gastro‐enteritis (AGE) in cats, and detected at high prevalence (47.0%) in faecal samples. Whether this finding was anecdotal or similar viruses are common components of feline virome is still unclear. Also, the potential impact of this virus on feline health is uncertain. Herewith, a case‐control study was performed to investigate whether this novel parvovirus may play a role as enteric pathogen, screening samples collected from cats with and without AGE signs. Furthermore, we extended the research by testing archival paired oropharyngeal and ocular samples collected from cats with or without upper respiratory tract disease (URTD). FeChPV DNA was detected at high prevalence rate (36.8%, 14/38) in clinical cases, representing the most frequently identified enteric virus, followed by feline panleukopenia parvovirus (23.7%, 9/38), feline coronavirus (5.3%, 2/38), feline kobuvirus (5.3%, 2/38) and noroviruses (5.3%, 2/38). The different prevalence rates of FeChPV between the case and control group were statistically significant, suggesting a possible association of the virus with acute gastro‐enteric disease. The virus was also detected at low rate in the respiratory samples of cats with (3.3%, 6/183) or without URTD (4.3%, 6/140), although there was no significant association between FeChPV and URTD. The complete VP encoding gene was determined for five viruses and the nearly full‐length genome was reconstructed for three viruses, namely 313R/2019/ITA, 284R/2019/ITA and 49E/2019/ITA. In the NS1‐based tree the Italian strains clustered tightly with the two FeChPV prototypes detected in Canada, within a monophyletic cluster related to but clearly distinct from canine chaphamaparvovirus, currently classified in the species Carnivore chaphamaparvovirus 1 (CaChPV‐1).
Federica Di Profio; Vittorio Sarchese; Andrea Palombieri; Paola Fruci; Ivano Massirio; Vito Martella; Marsilio Fulvio; Barbara Di Martino. Feline chaphamaparvovirus in cats with enteritis and upper respiratory tract disease. Transboundary and Emerging Diseases 2021, 1 .
AMA StyleFederica Di Profio, Vittorio Sarchese, Andrea Palombieri, Paola Fruci, Ivano Massirio, Vito Martella, Marsilio Fulvio, Barbara Di Martino. Feline chaphamaparvovirus in cats with enteritis and upper respiratory tract disease. Transboundary and Emerging Diseases. 2021; ():1.
Chicago/Turabian StyleFederica Di Profio; Vittorio Sarchese; Andrea Palombieri; Paola Fruci; Ivano Massirio; Vito Martella; Marsilio Fulvio; Barbara Di Martino. 2021. "Feline chaphamaparvovirus in cats with enteritis and upper respiratory tract disease." Transboundary and Emerging Diseases , no. : 1.
In industrialized countries, increasing autochthonous infections of hepatitis E virus (HEV) are caused by zoonotic transmission of genotypes (Gts) 3 and 4, mainly through consumption of contaminated raw or undercooked pork meat. Although swine and wild boar are recognized as the main reservoir for Gt3 and Gt4, accumulating evidence indicates that other animal species, including domestic and wild ruminants, may harbor HEV. Herein, we screened molecularly and serologically serum and fecal samples from two domestic and four wild ruminant species collected in Valle d’Aosta and Piemonte regions (northwestern Italy. HEV antibodies were found in sheep (21.6%), goats (11.4%), red deer (2.6%), roe deer (3.1%), and in Alpine ibex (6.3%). Molecular screening was performed using different primer sets targeting highly conserved regions of hepeviruses and HEV RNA, although at low viral loads, was detected in four fecal specimens (3.0%, 4/134) collected from two HEV seropositive sheep herds. Taken together, the data obtained document the circulation of HEV in the geographical area assessed both in wild and domestic ruminants, but with the highest seroprevalence in sheep and goats. Consistently with results from other studies conducted in southern Italy, circulation of HEV among small domestic ruminants seems to occur more frequently than expected.
Andrea Palombieri; Serena Robetto; Federica Di Profio; Vittorio Sarchese; Paola Fruci; Maria Cristina Bona; Giuseppe Ru; Riccardo Orusa; Fulvio Marsilio; Vito Martella; Barbara Di Martino. Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern Italy. Animals 2020, 10, 2351 .
AMA StyleAndrea Palombieri, Serena Robetto, Federica Di Profio, Vittorio Sarchese, Paola Fruci, Maria Cristina Bona, Giuseppe Ru, Riccardo Orusa, Fulvio Marsilio, Vito Martella, Barbara Di Martino. Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern Italy. Animals. 2020; 10 (12):2351.
Chicago/Turabian StyleAndrea Palombieri; Serena Robetto; Federica Di Profio; Vittorio Sarchese; Paola Fruci; Maria Cristina Bona; Giuseppe Ru; Riccardo Orusa; Fulvio Marsilio; Vito Martella; Barbara Di Martino. 2020. "Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern Italy." Animals 10, no. 12: 2351.
Canine chaphamaparvovirus (CaChPV) is a newly recognised parvovirus discovered by metagenomic analysis during an outbreak of diarrhoea in dogs in Colorado, USA, in 2017 and more recently detected in diarrhoeic dogs in China. Whether the virus plays a role as canine pathogen and whether it is distributed elsewhere, in other geographical areas, is not known. We performed a case-control study to investigate the possible association of CaChPV with enteritis in dogs. CaChPV DNA was detected both in the stools of diarrhoeic dogs (1.9 %, 3/155) and of healthy animals (1.6 %, 2/120). All the CaChPV-infected dogs with diarrhea were mixed infected with other enteric viruses such as canine parvovirus (formerly CPV-2), canine bufavirus (CBuV) and canine coronavirus (CCoV), whilst none of the asymptomatic CaChPV positive animals resulted co-infected. The nearly full-length genome and the partial capsid protein (VP) gene of three canine strains, Te/36OVUD/19/ITA, Te/37OVUD/19/ITA and Te/70OVUD/19/ITA, were reconstructed. Upon phylogenetic analyses based on the NS1 and VP aa sequences, the Italian CaChPV strains tightly clustered with the American reference viruses. Distinctive residues could be mapped to the deduced variable regions of the VP of canine and feline chaphamaparvoviruses, considered as important markers of host range and pathogenicity for parvoviruses.
Andrea Palombieri; Federica Di Profio; Gianvito Lanave; Paolo Capozza; Fulvio Marsilio; Vito Martella; Barbara Di Martino. Molecular detection and characterization of Carnivore chaphamaparvovirus 1 in dogs. Veterinary Microbiology 2020, 251, 108878 -108878.
AMA StyleAndrea Palombieri, Federica Di Profio, Gianvito Lanave, Paolo Capozza, Fulvio Marsilio, Vito Martella, Barbara Di Martino. Molecular detection and characterization of Carnivore chaphamaparvovirus 1 in dogs. Veterinary Microbiology. 2020; 251 ():108878-108878.
Chicago/Turabian StyleAndrea Palombieri; Federica Di Profio; Gianvito Lanave; Paolo Capozza; Fulvio Marsilio; Vito Martella; Barbara Di Martino. 2020. "Molecular detection and characterization of Carnivore chaphamaparvovirus 1 in dogs." Veterinary Microbiology 251, no. : 108878-108878.
Canine bufavirus (CBuV) is a protoparvovirus, genetically related to human and non‐human primate bufaviruses and distantly related to canine parvovirus type‐2 (CPV‐2). CBuV was initially identified from young dogs with respiratory signs but subsequent studies revealed that this virus is also a common component of the canine enteric virome. In this survey, by assessing archival and recent collections of dogs faecal samples, CBuV DNA was detected with a higher prevalence rate (8.8%) in animals with enteritis than in control animals (5.0%), although this difference was not statistically significant. The rate of co‐infections with other enteric viruses in diarrhoeic dogs was high (84.6%), mostly in association with canine parvovirus CPV‐2 (90.1%). The complete ORF2 gene was determined in five samples and the nearly full‐length genome was reconstructed for three strains, 62/2017/ITA, 9AS/2005/ITA and 35/2018/ITA. Upon sequence comparison, the viruses appeared highly conserved in the NS1 (97.2‐97.9% nt and 97.5‐98.1% aa identities). In the complete VP2 coding region, three strains were similar to the prototype viruses (99.7‐99.8 nt and 99.6‐99.8% aa) whilst strains 9AS/2005/ITA and 35/2016/ITA were distantly related (87.6‐89.3% nt and 93.9‐95.1% aa identities). Interestingly, genetic diversification occurred downstream conserved regions such as the VP1/VP2 splicing signals and/or the G‐rich motif in the N terminus of the VP2, suggesting a potential recombination nature. Upon phylogenetic analysis, the two divergent CBuV strains formed a distinct cluster/genotype.
Barbara Di Martino; Vittorio Sarchese; Federica Di Profio; Andrea Palombieri; Irene Melegari; Paola Fruci; Giovanni Aste; Krisztián Bányai; Marsilio Fulvio; Vito Martella. Genetic heterogeneity of canine bufaviruses. Transboundary and Emerging Diseases 2020, 68, 802 -812.
AMA StyleBarbara Di Martino, Vittorio Sarchese, Federica Di Profio, Andrea Palombieri, Irene Melegari, Paola Fruci, Giovanni Aste, Krisztián Bányai, Marsilio Fulvio, Vito Martella. Genetic heterogeneity of canine bufaviruses. Transboundary and Emerging Diseases. 2020; 68 (2):802-812.
Chicago/Turabian StyleBarbara Di Martino; Vittorio Sarchese; Federica Di Profio; Andrea Palombieri; Irene Melegari; Paola Fruci; Giovanni Aste; Krisztián Bányai; Marsilio Fulvio; Vito Martella. 2020. "Genetic heterogeneity of canine bufaviruses." Transboundary and Emerging Diseases 68, no. 2: 802-812.
Hepatitis E virus (HEV) infections constitute a significant health problem worldwide. The burden of hepatitis E in Italy seems low when compared with other European countries. In recent years, improved surveillance activities in Italy have revealed marked geographical differences in HEV epidemiology, with some regions characterised by higher seroprevalence rates. Abruzzo Region (Southern Italy) is currently recognised as a high-risk area for HEV infection. In this study, we investigated the epidemiology of HEV in Teramo Province by monitoring four wastewater treatment plants (WWTPs). Out of 56 influent sewage specimens collected during 2016-2017, HEV RNA was detected in 13/56 (23.2%) sewage samples from all the four WWTPs. Upon sequence analysis of the partial ORF2 gene, four strains showed the highest nucleotide identity to Gt3 subtype c, being more closely related to other HEVs previously identified in human and animal hosts in Abruzzo. For one strain, sequence data were generated only for a short region of the ORF1 gene, revealing the highest identity to HEVs Gt3 of subtype f. Altogether, the findings of this study confirm that HEV largely circulates in the setting investigated.
Federica Di Profio; Irene Melegari; Andrea Palombieri; Vittorio Sarchese; Alessio Arbuatti; Paola Fruci; Fulvio Marsilio; Vito Martella; Barbara Di Martino. High prevalence of hepatitis E virus in raw sewage in Southern Italy. Virus Research 2019, 272, 197710 .
AMA StyleFederica Di Profio, Irene Melegari, Andrea Palombieri, Vittorio Sarchese, Alessio Arbuatti, Paola Fruci, Fulvio Marsilio, Vito Martella, Barbara Di Martino. High prevalence of hepatitis E virus in raw sewage in Southern Italy. Virus Research. 2019; 272 ():197710.
Chicago/Turabian StyleFederica Di Profio; Irene Melegari; Andrea Palombieri; Vittorio Sarchese; Alessio Arbuatti; Paola Fruci; Fulvio Marsilio; Vito Martella; Barbara Di Martino. 2019. "High prevalence of hepatitis E virus in raw sewage in Southern Italy." Virus Research 272, no. : 197710.
Recent advances in the diagnostic and metagenomic investigations of the feline enteric environment have allowed the identification of several novel viruses that have been associated with gastroenteritis in cats. In the last few years, noroviruses, kobuviruses, and novel parvoviruses have been repetitively detected in diarrheic cats as alone or in mixed infections with other pathogens, raising a number of questions, with particular regards to their pathogenic attitude and clinical impact. In the present article, the current available literature on novel potential feline enteric viruses is reviewed, providing a meaningful update on the etiology, epidemiologic, pathogenetic, clinical, and diagnostic aspects of the infections caused by these pathogens.
Barbara Di Martino; Federica Di Profio; Irene Melegari; Fulvio Marsilio; Di Martino; Di Profio. Feline Virome—A Review of Novel Enteric Viruses Detected in Cats. Viruses 2019, 11, 908 .
AMA StyleBarbara Di Martino, Federica Di Profio, Irene Melegari, Fulvio Marsilio, Di Martino, Di Profio. Feline Virome—A Review of Novel Enteric Viruses Detected in Cats. Viruses. 2019; 11 (10):908.
Chicago/Turabian StyleBarbara Di Martino; Federica Di Profio; Irene Melegari; Fulvio Marsilio; Di Martino; Di Profio. 2019. "Feline Virome—A Review of Novel Enteric Viruses Detected in Cats." Viruses 11, no. 10: 908.
Novel protoparvoviruses genetically related to human and non-human primate bufaviruses (BuVs) have been detected recently in respiratory and enteric specimens collected from dogs and cats. In this study, by molecular screening of archival collections of faecal samples from wolves and foxes, we detected BuVs with a rate of 17.1% (7/41) and 10.5% (9/86), respectively. Sequence analysis of a portion of the ORF2 gene region of nine positive samples showed that the viruses in these samples were closely related to BuVs (97.5–99.0% nucleotide sequence identity) found in domestic carnivores.
Irene Melegari; Federica Di Profio; Andrea Palombieri; Vittorio Sarchese; Georgia Diakoudi; Serena Robetto; Riccardo Orusa; Fulvio Marsilio; Krisztián Bányai; Vito Martella; Barbara Di Martino. Molecular detection of canine bufaviruses in wild canids. Archives of Virology 2019, 164, 2315 -2320.
AMA StyleIrene Melegari, Federica Di Profio, Andrea Palombieri, Vittorio Sarchese, Georgia Diakoudi, Serena Robetto, Riccardo Orusa, Fulvio Marsilio, Krisztián Bányai, Vito Martella, Barbara Di Martino. Molecular detection of canine bufaviruses in wild canids. Archives of Virology. 2019; 164 (9):2315-2320.
Chicago/Turabian StyleIrene Melegari; Federica Di Profio; Andrea Palombieri; Vittorio Sarchese; Georgia Diakoudi; Serena Robetto; Riccardo Orusa; Fulvio Marsilio; Krisztián Bányai; Vito Martella; Barbara Di Martino. 2019. "Molecular detection of canine bufaviruses in wild canids." Archives of Virology 164, no. 9: 2315-2320.
Hepatitis E virus (HEV) is the leading cause of human enterically transmitted viral hepatitis occurring around the world both as outbreaks and as sporadic cases. The accumulating literature indicates that domestic pigs and wild boars are the main reservoirs of genotype 3 and genotype 4 for human infections in industrialized countries. However, the recent identification of HEV from various animal species poses additional potential concerns for HEV zoonotic infection. In this study, the role of sheep as potential host of hepatitis E virus (HEV) was investigated. By screening 192 sheep from seven farms located in Abruzzo Region (Southern Italy), HEV-specific antibodies were detected in the sera of 41 animals (21.3%) whilst the RNA of HEV, genotype 3, was detected in 20 faecal (10.4%) and three serum samples (1.6%). Upon sequence analyses of a partial ORF2 gene region of eight HEV positive samples, the sheep sequences all grouped together within HEV genotype 3 subtype c, being most closely related to HEV strains identified in goat and wild boar from Abruzzo. This is the first study that demonstrates, serologically and molecularly, the presence of HEV in sheep population in a European country.
Vittorio Sarchese; Federica Di Profio; Irene Melegari; Andrea Palombieri; Sandra Bermúdez Sánchez; Alessio Arbuatti; Massimo Ciuffetelli; Fulvio Marsilio; Vito Martella; Barbara Di Martino. Hepatitis E virus in sheep in Italy. Transboundary and Emerging Diseases 2019, 66, 1120 -1125.
AMA StyleVittorio Sarchese, Federica Di Profio, Irene Melegari, Andrea Palombieri, Sandra Bermúdez Sánchez, Alessio Arbuatti, Massimo Ciuffetelli, Fulvio Marsilio, Vito Martella, Barbara Di Martino. Hepatitis E virus in sheep in Italy. Transboundary and Emerging Diseases. 2019; 66 (3):1120-1125.
Chicago/Turabian StyleVittorio Sarchese; Federica Di Profio; Irene Melegari; Andrea Palombieri; Sandra Bermúdez Sánchez; Alessio Arbuatti; Massimo Ciuffetelli; Fulvio Marsilio; Vito Martella; Barbara Di Martino. 2019. "Hepatitis E virus in sheep in Italy." Transboundary and Emerging Diseases 66, no. 3: 1120-1125.
Noroviruses (NoVs) are a major cause of epidemic gastroenteritis in children and adults. Several pieces of evidence suggest that viruses genetically and antigenically closely related to human NoVs might infect animals, raising public health concerns about potential cross‐species transmission. The natural susceptibility of non‐human primates (NPHs) to human NoV infections has already been reported, but a limited amount of data is currently available. In order to start filling this gap, we screened a total of 86 serum samples of seven different species of NPHs housed at the Zoological Garden (Bioparco) of Rome (Italy), collected between 2001 and 2017, using an enzyme‐linked immunosorbent assay (ELISA) based on virus‐like particles (VLPs) of human GII.4 and GIV.1 NoVs. Antibodies specific for both genotypes were detected with an overall prevalence of 32.6%. In detail, IgG antibodies against GII.4 NoVs were found in 18 Japanese macaques (29.0%, 18/62), a mandrill (10.0%, 1/10), a white‐crowned mangabey (16.6%, 1/6) and in an orangutan (33.3%, 1/3). Twelve macaques (19.3%, 12/62), five mandrills (50.0%, 5/10), two chimpanzees (100%, 2/2) and a white‐crowned mangabey (16.6%, 1/6) showed antibodies for GIV.1 NoVs. The findings of this study confirm the natural susceptibility of captive NHPs to GII NoV infections. In addition, IgG antibodies against GIV.1 were detected, suggesting that NHPs are exposed to GIV NoVs or to antigenically related NoV strains.
Federica Di Profio; Vittorio Sarchese; Irene Melegari; Andrea Palombieri; Ivano Massirio; Sandra Bermúdez Sánchez; Klaus Gunther Friedrich; Federico Coccia; Fulvio Marsilio; Vito Martella; Barbara Di Martino. Seroprevalence for norovirus genogroups GII and GIV in captive non‐human primates. Zoonoses and Public Health 2019, 66, 310 -315.
AMA StyleFederica Di Profio, Vittorio Sarchese, Irene Melegari, Andrea Palombieri, Ivano Massirio, Sandra Bermúdez Sánchez, Klaus Gunther Friedrich, Federico Coccia, Fulvio Marsilio, Vito Martella, Barbara Di Martino. Seroprevalence for norovirus genogroups GII and GIV in captive non‐human primates. Zoonoses and Public Health. 2019; 66 (3):310-315.
Chicago/Turabian StyleFederica Di Profio; Vittorio Sarchese; Irene Melegari; Andrea Palombieri; Ivano Massirio; Sandra Bermúdez Sánchez; Klaus Gunther Friedrich; Federico Coccia; Fulvio Marsilio; Vito Martella; Barbara Di Martino. 2019. "Seroprevalence for norovirus genogroups GII and GIV in captive non‐human primates." Zoonoses and Public Health 66, no. 3: 310-315.
Hepatitis E virus (HEV) is the leading cause of human enterically-transmitted viral hepatitis occurring around the world both as outbreaks and as sporadic cases. Non-human primates (NHPs) have been experimentally infected with HEV, but few studies have been reported about natural infection in wild-living and zoo monkeys. In order to provide a more complete picture on the epidemiology of HEV in NHPs living in controlled environment, we investigated the presence of HEV by screening serologically and molecularly a historical collection of 86 sera from seven different species of primates housed at the Zoological Garden (Bioparco) of Rome, Italy. By using an enzyme-linked immunosorbent assay (ELISA) based on the recombinant capsid protein of a Gt3 HEV strain, IgG antibodies were detected in three macaques (4.8%; 3/62) and in a white-crowned mangabey (16.6%; 1/6), with an overall prevalence of 4.6% (4/86). This positivity was confirmed when assessed the sera by western blotting. Rescreening the sera for IgM and viral RNA, all the samples resulted negative. Also, HEV RNA was not found when 17 stool samples were analyzed by RT-PCR. Although these results suggest that none of the monkeys housed at the Bioparco of Rome in the 17-year time frame spanning 2001 to 2017 developed acute or at least sub-acute HEV disease, the detection of IgG antibodies demonstrated that animals living in this setting were exposed to HEV or to antigenically related viruses.
Irene Melegari; Federica Di Profio; Fulvio Marsilio; Vittorio Sarchese; Andrea Palombieri; Klaus Gunther Friedrich; Federico Coccia; Barbara Di Martino. Serological and molecular investigation for hepatitis E virus (HEV) in captive non-human primates, Italy. Virus Research 2018, 251, 17 -21.
AMA StyleIrene Melegari, Federica Di Profio, Fulvio Marsilio, Vittorio Sarchese, Andrea Palombieri, Klaus Gunther Friedrich, Federico Coccia, Barbara Di Martino. Serological and molecular investigation for hepatitis E virus (HEV) in captive non-human primates, Italy. Virus Research. 2018; 251 ():17-21.
Chicago/Turabian StyleIrene Melegari; Federica Di Profio; Fulvio Marsilio; Vittorio Sarchese; Andrea Palombieri; Klaus Gunther Friedrich; Federico Coccia; Barbara Di Martino. 2018. "Serological and molecular investigation for hepatitis E virus (HEV) in captive non-human primates, Italy." Virus Research 251, no. : 17-21.
Caliciviruses of the Sapovirus genus have been recently detected in dogs. Canine sapoviruses (SaVs) have been identified in the stools of young or juvenile animals with gastro-enteric disease at low prevalence (2.0–2.2%), but whether they may have a role as enteric pathogens and to which extent dogs are exposed to SaVs remains unclear. Here, we report the expression in a baculovirus system of virus like-particles (VLPs) of a canine SaV strain, the prototype virus Bari/4076/2007/ITA. The recombinant antigen was used to develop an enzyme-linked immunosorbent assay (ELISA). By screening an age-stratified collection of serum samples from 516 dogs in Italy, IgG antibodies specific for the canine SaV VLPs were detected in 40.3% (208/516) of the sera. Also, as observed for SaV infection in humans, we observed a positive association between seropositivity and age, with the highest prevalence rates in dogs older than 4 years of age.
Irene Melegari; Fulvio Marsilio; Federica Di Profio; Vittorio Sarchese; Ivano Massirio; Andrea Palombieri; Anna Rita D'Angelo; Gianvito Lanave; Georgia Diakoudi; Alessandra Cavalli; Vito Martella; Barbara Di Martino. Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles. Virus Research 2018, 251, 1 -5.
AMA StyleIrene Melegari, Fulvio Marsilio, Federica Di Profio, Vittorio Sarchese, Ivano Massirio, Andrea Palombieri, Anna Rita D'Angelo, Gianvito Lanave, Georgia Diakoudi, Alessandra Cavalli, Vito Martella, Barbara Di Martino. Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles. Virus Research. 2018; 251 ():1-5.
Chicago/Turabian StyleIrene Melegari; Fulvio Marsilio; Federica Di Profio; Vittorio Sarchese; Ivano Massirio; Andrea Palombieri; Anna Rita D'Angelo; Gianvito Lanave; Georgia Diakoudi; Alessandra Cavalli; Vito Martella; Barbara Di Martino. 2018. "Seroprevalence of sapovirus in dogs using baculovirus-expressed virus-like particles." Virus Research 251, no. : 1-5.
Federica Di Profio; Irene Melegari; Vittorio Sarchese; Serena Robetto; Sandra Bermúdez Sánchez; Emanuele Carella; Riccardo Orusa; Patrizia Cavadini; Antonio Lavazza; Fulvio Marsilio; Vito Martella; Barbara Di Martino. Potential role of wolf ( Canis lupus ) as passive carrier of European brown hare syndrome virus (EBHSV). Research in Veterinary Science 2018, 117, 81 -84.
AMA StyleFederica Di Profio, Irene Melegari, Vittorio Sarchese, Serena Robetto, Sandra Bermúdez Sánchez, Emanuele Carella, Riccardo Orusa, Patrizia Cavadini, Antonio Lavazza, Fulvio Marsilio, Vito Martella, Barbara Di Martino. Potential role of wolf ( Canis lupus ) as passive carrier of European brown hare syndrome virus (EBHSV). Research in Veterinary Science. 2018; 117 ():81-84.
Chicago/Turabian StyleFederica Di Profio; Irene Melegari; Vittorio Sarchese; Serena Robetto; Sandra Bermúdez Sánchez; Emanuele Carella; Riccardo Orusa; Patrizia Cavadini; Antonio Lavazza; Fulvio Marsilio; Vito Martella; Barbara Di Martino. 2018. "Potential role of wolf ( Canis lupus ) as passive carrier of European brown hare syndrome virus (EBHSV)." Research in Veterinary Science 117, no. : 81-84.
The genus Staphylococcus consists of a variety of opportunistic pathogens of diverse relevance in veterinary medicine. Staphylococci are divided into coagulase-positive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS) based on their ability to coagulate rabbit plasma. Another property of these organisms is their capacity to become resistant to antimicrobials. Methicillin resistance is of particular relevance because it is conferred by the mecA gene, which encodes for production of an altered penicillin binding protein that has a low affinity for all beta-lactam antibiotics (penicillins, cephalosporins, carbapenems). CoNS are an important mecA reservoir that have the potential to be transferred to susceptible Staphylococcus strains, including Staphylococcus aureus. Therefore, methicillin-resistant staphylococci can be resistant to a broad range of antimicrobials. The most clinically relevant staphylococci in animals are CoPS belonging to S. aureus along with members of the Staphylococcus intermedius group, particularly Staphylococcus pseudintermedius (the group also includes S. intermedius and Staphylococcus delphini). S. aureus is mainly involved in bovine mastitis (which is considered the leading cause of economic losses in dairy cattle herds) and can occasionally inhabit the skin and mucous membrane of dogs, too. In dairy farms that have successfully controlled S. aureus-related mastitis, CoNS became a frequent cause of bovine mammary gland infections. Conversely, dogs represent the main S. pseudintermedius reservoir, with carriage rates varying based on the diverse body-sites. Although S. aureus and S. pseudintermedius are mostly recovered from cattle and dogs, their presence may be sometimes associated with human contact.
Fulvio Marsilio; Cristina E. Di Francesco; Barbara Di Martino. Coagulase-Positive and Coagulase-Negative Staphylococci Animal Diseases. Pet-To-Man Travelling Staphylococci 2018, 43 -50.
AMA StyleFulvio Marsilio, Cristina E. Di Francesco, Barbara Di Martino. Coagulase-Positive and Coagulase-Negative Staphylococci Animal Diseases. Pet-To-Man Travelling Staphylococci. 2018; ():43-50.
Chicago/Turabian StyleFulvio Marsilio; Cristina E. Di Francesco; Barbara Di Martino. 2018. "Coagulase-Positive and Coagulase-Negative Staphylococci Animal Diseases." Pet-To-Man Travelling Staphylococci , no. : 43-50.
Anna Białecka; Joanna Białecka; Emilia Bonar; Aneta Buda; Michał Bukowski; Edoardo Carretto; Danuta Czernomysy-Furowicz; Giovanni Di Bonaventura; Cristina E. Di Francesco; Barbara Di Martino; Adam Dubin; Grzegorz Dubin; Marco Favaro; Paolo Fazii; Karol Fijałkowski; Carla Fontana; Giovanni Gherardi; Stefania Giedrys-Kalemba; Weronika M. Ilczyszyn; Joanna Jursa-Kulesza; Jolanta Karakulska; Andrzej Kasprowicz; Paweł Kaszycki; Maja Kosecka-Strojek; Beata Krawczyk; Józef Kur; Aldo Lepidi; Clemencia Chaves López; Paweł Mak; Roberta Marrollo; Fulvio Marsilio; Giovanni Mazzarrino; Jacek Miedzobrodzki; Paola Nardini; Paweł Nawrotek; Antonello Paparella; Eugenio Pontieri; Chiara Rossi; Barbara Rózalska; Beata Sadowska; Vincenzo Savini; Annalisa Serio; Natalia Stach; Rosa Visiello; Benedykt Władyka. Contributors. Pet-To-Man Travelling Staphylococci 2018, 1 .
AMA StyleAnna Białecka, Joanna Białecka, Emilia Bonar, Aneta Buda, Michał Bukowski, Edoardo Carretto, Danuta Czernomysy-Furowicz, Giovanni Di Bonaventura, Cristina E. Di Francesco, Barbara Di Martino, Adam Dubin, Grzegorz Dubin, Marco Favaro, Paolo Fazii, Karol Fijałkowski, Carla Fontana, Giovanni Gherardi, Stefania Giedrys-Kalemba, Weronika M. Ilczyszyn, Joanna Jursa-Kulesza, Jolanta Karakulska, Andrzej Kasprowicz, Paweł Kaszycki, Maja Kosecka-Strojek, Beata Krawczyk, Józef Kur, Aldo Lepidi, Clemencia Chaves López, Paweł Mak, Roberta Marrollo, Fulvio Marsilio, Giovanni Mazzarrino, Jacek Miedzobrodzki, Paola Nardini, Paweł Nawrotek, Antonello Paparella, Eugenio Pontieri, Chiara Rossi, Barbara Rózalska, Beata Sadowska, Vincenzo Savini, Annalisa Serio, Natalia Stach, Rosa Visiello, Benedykt Władyka. Contributors. Pet-To-Man Travelling Staphylococci. 2018; ():1.
Chicago/Turabian StyleAnna Białecka; Joanna Białecka; Emilia Bonar; Aneta Buda; Michał Bukowski; Edoardo Carretto; Danuta Czernomysy-Furowicz; Giovanni Di Bonaventura; Cristina E. Di Francesco; Barbara Di Martino; Adam Dubin; Grzegorz Dubin; Marco Favaro; Paolo Fazii; Karol Fijałkowski; Carla Fontana; Giovanni Gherardi; Stefania Giedrys-Kalemba; Weronika M. Ilczyszyn; Joanna Jursa-Kulesza; Jolanta Karakulska; Andrzej Kasprowicz; Paweł Kaszycki; Maja Kosecka-Strojek; Beata Krawczyk; Józef Kur; Aldo Lepidi; Clemencia Chaves López; Paweł Mak; Roberta Marrollo; Fulvio Marsilio; Giovanni Mazzarrino; Jacek Miedzobrodzki; Paola Nardini; Paweł Nawrotek; Antonello Paparella; Eugenio Pontieri; Chiara Rossi; Barbara Rózalska; Beata Sadowska; Vincenzo Savini; Annalisa Serio; Natalia Stach; Rosa Visiello; Benedykt Władyka. 2018. "Contributors." Pet-To-Man Travelling Staphylococci , no. : 1.
Canine kobuviruses (CaKoVs) were first identified in diarrhoeic and asymptomatic dogs in 2011 in the USA. Subsequent studies have demonstrated a worldwide distribution of these viruses, but it is not clear if CaKoVs play a role as enteric pathogens of dogs. More recently, CaKoV RNA has been detected in wild carnivores, including red fox, golden jackal, side-striped jackal and spotted hyena. In this study, we addressed the hypothesis that wolves are susceptible to CaKoV infections. A total of 185 wolf stool samples were collected from necropsied animals and from transects in the Liguria, Piemonte and Valle D’Aosta regions of Italy, and CaKoV RNA was identified in two of these specimens. Both samples were obtained from necropsied wolves, with a prevalence rate of 4.9% (2/41). Sequence analysis of the full-length VP1 region showed that these strains displayed the highest nucleotide (nt) sequence identity (86.3-98.5%) to canine strains identified in the UK and Africa, and to kobuviruses that were previously detected in other African wild carnivores. This suggests that genetically related CaKoV strains circulate in domestic and wild carnivores, with interspecies transmission being not uncommon among carnivores of different ecosystems.
Irene Melegari; Vittorio Sarchese; Federica Di Profio; Serena Robetto; Emanuele Carella; Sandra Bermúdez Sánchez; Riccardo Orusa; Vito Martella; Fulvio Marsilio; Barbara Di Martino. First molecular identification of kobuviruses in wolves (Canis lupus) in Italy. Archives of Virology 2017, 163, 509 -513.
AMA StyleIrene Melegari, Vittorio Sarchese, Federica Di Profio, Serena Robetto, Emanuele Carella, Sandra Bermúdez Sánchez, Riccardo Orusa, Vito Martella, Fulvio Marsilio, Barbara Di Martino. First molecular identification of kobuviruses in wolves (Canis lupus) in Italy. Archives of Virology. 2017; 163 (2):509-513.
Chicago/Turabian StyleIrene Melegari; Vittorio Sarchese; Federica Di Profio; Serena Robetto; Emanuele Carella; Sandra Bermúdez Sánchez; Riccardo Orusa; Vito Martella; Fulvio Marsilio; Barbara Di Martino. 2017. "First molecular identification of kobuviruses in wolves (Canis lupus) in Italy." Archives of Virology 163, no. 2: 509-513.
Vesivirus 2117 was first discovered as a contaminant in Chinese hamster ovary (CHO) cell cultures used for human drug production. Similar vesiviruses (VeVs) have been detected recently in dogs. In order to address the hypothesis that cats may also be exposed to 2117-like VeVs, in this study, we screened 236 feline sera using an enzyme-linked immunosorbent assay (ELISA) based on a recombinant VP1 protein from the canine VeV Bari/212/07/ITA. IgG antibodies against the 2117-like VeV were detected in 37.3% of the sera tested. Also, by screening cat faecal specimens, the RNA of a 2117-like VeV was detected in a clinically healthy cat.
Barbara Di Martino; Federica Di Profio; Irene Melegari; Vittorio Sarchese; Ivano Massirio; Alessia Luciani; Gianvito Lanave; Fulvio Marsilio; Vito Martella. Serological and molecular investigation of 2117-like vesiviruses in cats. Archives of Virology 2017, 163, 197 -201.
AMA StyleBarbara Di Martino, Federica Di Profio, Irene Melegari, Vittorio Sarchese, Ivano Massirio, Alessia Luciani, Gianvito Lanave, Fulvio Marsilio, Vito Martella. Serological and molecular investigation of 2117-like vesiviruses in cats. Archives of Virology. 2017; 163 (1):197-201.
Chicago/Turabian StyleBarbara Di Martino; Federica Di Profio; Irene Melegari; Vittorio Sarchese; Ivano Massirio; Alessia Luciani; Gianvito Lanave; Fulvio Marsilio; Vito Martella. 2017. "Serological and molecular investigation of 2117-like vesiviruses in cats." Archives of Virology 163, no. 1: 197-201.
Molecular and serological data suggest that noroviruses (NoVs) might be transmitted between humans and domestic carnivores. In this study we screened an age-stratified collection of canine sera (n=516) by using an ELISA assay based on virus-like particles (VLPs) of human NoVs GII.4 and GIV.1 and carnivore NoVs GIV.2 and GVI.2. Antibodies against GII.4 and GIV.1 human NoVs and GIV.2 and GVI.2 NoVs from carnivores were identified in dog sera (13.0%, 67/516) suggesting their exposure to homologous and heterologous NoVs. Analysis of the trends of age-class prevalence showed a gradual increase in the positive rate from 9.0% and 7.0%, in young dogs <1year of age to 15.0% in dogs older than 12 years, for GII.4 and GVI.2 NoVs, respectively. A significant difference in the IgG distribution by age classes was observed for GIV.1 NoVs, with the highest rate of antibodies (7.0%) in the age group <1year and the lowest (1.0%) in the age-classes 7-9 (P=0.049). High correlation between the reactivity to GII.4 and GVI.2 NoVs was observed, likely due to conserved epitopes in the capsid structure.
Barbara Di Martino; Federica Di Profio; Irene Melegari; Vittorio Sarchese; Ivano Massirio; Giuseppe Palermo; Giovanni Romito; Eleonora Lorusso; Gianvito Lanave; Livia Bodnar; Canio Buonavoglia; Fulvio Marsilio; Kim Y. Green; Vito Martella. Seroprevalence for norovirus genogroup II, IV and VI in dogs. Veterinary Microbiology 2017, 203, 68 -72.
AMA StyleBarbara Di Martino, Federica Di Profio, Irene Melegari, Vittorio Sarchese, Ivano Massirio, Giuseppe Palermo, Giovanni Romito, Eleonora Lorusso, Gianvito Lanave, Livia Bodnar, Canio Buonavoglia, Fulvio Marsilio, Kim Y. Green, Vito Martella. Seroprevalence for norovirus genogroup II, IV and VI in dogs. Veterinary Microbiology. 2017; 203 ():68-72.
Chicago/Turabian StyleBarbara Di Martino; Federica Di Profio; Irene Melegari; Vittorio Sarchese; Ivano Massirio; Giuseppe Palermo; Giovanni Romito; Eleonora Lorusso; Gianvito Lanave; Livia Bodnar; Canio Buonavoglia; Fulvio Marsilio; Kim Y. Green; Vito Martella. 2017. "Seroprevalence for norovirus genogroup II, IV and VI in dogs." Veterinary Microbiology 203, no. : 68-72.