We investigated the removal of nitrogen and phosphate from the effluent of a sewage treatment plant over a long-term operation in bioreactors packed with different combinations of wood and iron, with a trickling filter packed with foam ceramics for nitrification. The average nitrification rate in the trickling filter was 0.17 kg N/m³∙day and remained at 0.11 kg N/m³∙day even when the water temperature was below 15 °C. The denitrification and phosphate removal rates in the bioreactor packed with aspen wood and iron were higher than those in the bioreactor packed with cedar chips and iron. The bioreactor packed with aspen wood and iron continued to remove nitrate and phosphate for >1200 days of operation. The nitrate removal activity of a biofilm attached to the aspen wood from the bioreactor after 784 days of operation was 0.42 g NO₃-N/kg dry weight wood∙ day. There was no increase in the amount of dissolved organic matter in the outflow from the bioreactors.

Metals are considered a suitable anode material for microbial fuel cells (MFCs) because of their high electrical conductivity. However, only a few types of metals have been used as anodes, and an extensive screening of metals has not yet been conducted. In this study, to develop a new metal anode for increased electricity generation in MFCs, 14 different metals (Al, Ti, Fe, Ni, Cu, Zn, Zr, Nb, Mo, Ag, In, Sn, Ta, and W) and 31 of their oxidized forms were comprehensively tested. Oxidized-metal anodes were prepared using flame oxidation, heat treatment, and electrochemical oxidation. The selected anodes were further evaluated in detail using air–cathode single-chambered MFCs. The untreated Mo and electrochemically oxidized Mo anodes showed high averages of maximum power densities in the screening test, followed by flame-oxidized (FO) W, FO-Fe, FO-Mo, and Sn-based anodes. The untreated Mo and FO-W anodes were selected for further evaluation. X-ray analyses revealed that the surface of the Mo anode was naturally oxidized in the presence of air, forming a layer of MoO3, a known oxidation catalyst. A high maximum power density (1296 mW/m2) was achieved using the Mo anode in the MFCs, which was superior to that obtained using the FO-W anode (1036 mW/m2). The Mo anode, but not the FO-W anode, continued to produce current without detectable corrosion until the end of operation (350 days). Geobacter was abundant in both biofilms on the Mo and FO-W anodes, as analyzed by high-throughput sequencing of the 16S rRNA gene. The screening test revealed that Mo, W, Fe, and Sn are useful MFC anode materials. The detailed analyses demonstrated that the Mo anode is a high-performance electrode with structural simplicity and long-term stability in MFCs. The anode can be easily prepared by merely shaping Mo materials to the desired forms. These properties would enable the large-scale preparation of the anode, required for practical MFC applications. This study also implies the potential involvement of Geobacter in the Mo and W cycles on Earth.

Livestock production is indicated to be one of the major emitters of greenhouse gases (GHG), particularly methane (CH₄) and nitrous oxide (N₂O), around the globe, and the reduction of these emissions is an important goal. GHG emissions as well as other environmental impacts of two pig (Sus scrofa domesticus) farming systems, one using conventional diets (CNV) and the other using low-protein diets supplemented with crystalline amino acids (LOW), were therefore evaluated by comparative life cycle assessment (LCA) focusing on manure management and by cradle-to-farm gate LCA. The functional unit was defined as one marketed pig. For the comparative LCA of manure management, the CH₄ and N₂O emissions from manure management of CNV were set as a baseline, and the system boundary of LOW included the CH₄ and N₂O emissions from manure management, and changes in the GHG emissions from feed production including amino acid manufacturing, feed transport, and the materials and energy consumed in manure management. For the cradle-to-farm gate LCA of pig farming, the evaluated system included the processes of feed production including amino acid manufacturing for LOW, feed transport, animal housing including the biological activity of the animal, and manure management. The results of the comparative LCA showed that the GHG emissions from manure management of LOW were 20% less than those of CNV, and the GHG reduction rate of LOW compared to CNV was even greater in the case of a stricter target of effluent nitrogen content. The results of cradle-to-farm gate LCA showed that LOW had lower GHG emissions, acidification potential, eutrophication potential and overall environmental impact, and slightly larger energy consumption, than CNV. The sensitivity analysis showed that LOW still had less GHG emissions than CNV, even in the least preferable case assuming a 40% lower reduction rate of nitrogen excretion.

Biochemical oxygen demand (BOD) is a widely used index of water-quality assessment. Since bioelectrochemical BOD biosensors require anaerobic conditions for anodic reactions, they are not directly used in aerobic environments such as aeration tanks. Normally, the BOD biosensors are closed-type, where the anode is packed inside a closed chamber to avoid exposure to oxygen. In this study, a novel bioelectrochemical open-type biosensor was designed for in-situ monitoring of BOD during intermittent aeration. The open-type anode, without any protection against exposure to oxygen, was directly inserted into an intermittently aerated tank filled with livestock wastewater. Anodic potential was controlled using a potentiostat. Interestingly, this novel biosensor generated similar levels of current under both aerating and non-aerating conditions, and showed a logarithmic correlation (R² > 0.9) of current with BOD concentrations up to 250 mg/L. Suspended solids in the wastewater attached to and covered the whole anode, presumably leading to the production of anaerobic conditions inside the covered anode via biological oxygen removal. Exoelectrogenic anaerobes (Geobacter spp.) were detected inside the covered anode using the 16S-rRNA gene. This biosensor will have various practical applications, such as the automatic control of aeration intensity and the in-situ monitoring of natural water environments.