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Alyona Sukhanova
Laboratoire de Recherche en Nanosciences (LRN-EA4682), Université de Reims Champagne-Ardenne, 51100 Reims, France

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Research article
Published: 14 July 2020 in ACS Applied Materials & Interfaces
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The layer-by-layer (LbL) deposition approach allows combined incorporation of fluorescent, magnetic, and plasmonic nanoparticles into the shell of polyelectrolyte microcapsules to obtain stimulus-responsive systems whose imaging and drug release functions can be triggered by external stimuli. The combined use of fluorescent quantum dots (QDs) and magnetic nanoparticles (MNPs) yields magnetic-field-driven imaging tools that can be tracked and imaged even deep in tissue when the appropriate type of QDs and wavelength of their excitation are used. QDs are excellent photonic labels for microcapsule encoding due to their close-to-unity photoluminescence (PL) quantum yields, narrow PL emission bands, and tremendous one- and two-photon extinction coefficients. However, the presence of MNPs and electrically charged polyelectrolyte molecules used for the LbL fabrication of magneto-optical microcapsules provokes alterations of the QD optical properties because of the photoinduced charge and energy transfer resulting in QD photodarkening or photobrightening. These lead to variation of the microcapsule PL signal under illumination, which hampers their tracking and quantitative analysis in cells and tissues. Here, we have studied the effects of the structure and spatial arrangement of the nanoparticles within the microcapsule polyelectrolyte shell, the total shell thickness, and the shell surface charge on their PL properties under continuous illumination. The roles of the charge transfer and its main driving forces in the stability of the microcapsules PL signal have been established, and the design of the microcapsules dually encoded with QDs and MNPs providing the strongest and most stable PL has been determined. Controlling the energy transfer from the QDs and MNPs and the charge transfer from QDs to polyelectrolyte layers in the engineering of magneto-optical microcapsules with a bright and stable PL signal extends their applications to long-lasting quantitative fluorescence imaging.

ACS Style

Galina Nifontova; Victor Krivenkov; Maria Zvaigzne; Pavel S. Samokhvalov; Anton E. Efimov; Olga I. Agapova; Igor I. Agapov; Evgeny Korostylev; Sergei Zarubin; Alexander Karaulov; Igor R. Nabiev; Alyona Sukhanova. Controlling Charge Transfer from Quantum Dots to Polyelectrolyte Layers Extends Prospective Applications of Magneto-Optical Microcapsules. ACS Applied Materials & Interfaces 2020, 12, 35882 -35894.

AMA Style

Galina Nifontova, Victor Krivenkov, Maria Zvaigzne, Pavel S. Samokhvalov, Anton E. Efimov, Olga I. Agapova, Igor I. Agapov, Evgeny Korostylev, Sergei Zarubin, Alexander Karaulov, Igor R. Nabiev, Alyona Sukhanova. Controlling Charge Transfer from Quantum Dots to Polyelectrolyte Layers Extends Prospective Applications of Magneto-Optical Microcapsules. ACS Applied Materials & Interfaces. 2020; 12 (32):35882-35894.

Chicago/Turabian Style

Galina Nifontova; Victor Krivenkov; Maria Zvaigzne; Pavel S. Samokhvalov; Anton E. Efimov; Olga I. Agapova; Igor I. Agapov; Evgeny Korostylev; Sergei Zarubin; Alexander Karaulov; Igor R. Nabiev; Alyona Sukhanova. 2020. "Controlling Charge Transfer from Quantum Dots to Polyelectrolyte Layers Extends Prospective Applications of Magneto-Optical Microcapsules." ACS Applied Materials & Interfaces 12, no. 32: 35882-35894.

Protocol
Published: 04 April 2020 in Cardiovascular Development
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A quantum dot (QD)-based lab-on-bead system is a unique tool for multiple analysis of cancer markers in human serum samples by using a flow cytometer. In terms of specificity and sensitivity, this method is comparable with ELISA, the “gold standard” of serological in-clinic detection of single analytes. Fluorescent microspheres encoded with QDs have been used for the quantitative detection of free and total prostate-specific antigen in human serum samples. Developed multiplex assay demonstrates a clear discrimination between serum samples from control subjects and cancer patients. The proposed QD-based method is adaptable and makes it possible to develop numerous clinical tests with decreased duration and cost for early diagnosis of various diseases.

ACS Style

Tatiana Tsoy; Alexander Karaulov; Igor Nabiev; Alyona Sukhanova. Multiplexed Detection of Cancer Serum Antigens with a Quantum Dot-Based Lab-on-Bead System. Cardiovascular Development 2020, 2135, 225 -236.

AMA Style

Tatiana Tsoy, Alexander Karaulov, Igor Nabiev, Alyona Sukhanova. Multiplexed Detection of Cancer Serum Antigens with a Quantum Dot-Based Lab-on-Bead System. Cardiovascular Development. 2020; 2135 ():225-236.

Chicago/Turabian Style

Tatiana Tsoy; Alexander Karaulov; Igor Nabiev; Alyona Sukhanova. 2020. "Multiplexed Detection of Cancer Serum Antigens with a Quantum Dot-Based Lab-on-Bead System." Cardiovascular Development 2135, no. : 225-236.

Protocol
Published: 04 April 2020 in Cardiovascular Development
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Fluorescent semiconductor nanocrystals, known as quantum dots (QDs), and magnetic nanoparticles (MNPs) are extensively studied perspective tools for optical (fluorescence) and magnetic resonance imaging techniques. The unique optical properties, high photostability, and bright luminescence of QDs make them more promising fluorophores than the classical organic dyes. Encoding polyelectrolyte microcapsules with QDs and MNPs ensures their sensitivity to both photoexcitation and magnetic field. This chapter presents the protocol for obtaining a stimulus-sensitive delivery system based on QD- and MNP-encoded polyelectrolyte microcapsules by means of layer-by-layer self-assembly. The resultant fluorescent magnetic polyelectrolyte microcapsules are 3.4–5.5 μm in size, have a hollow structure, and are brightly fluorescent to be detected with the standard imaging equipment. Polyelectrolyte microcapsule surface bears functional groups for subsequent functionalization with vector capture molecules. The polyelectrolyte microcapsules containing combination of QDs and MNPs are advanced visualization tools, since they can be sorted in a magnetic field and at the same time are suitable for fluorescent imaging what can be applied within a wide range of diagnostic and therapeutic protocols.

ACS Style

Galina Nifontova; Fernanda Ramos-Gomes; Frauke Alves; Igor Nabiev; Alyona Sukhanova. Stimulus-Sensitive Theranostic Delivery Systems Based on Microcapsules Encoded with Quantum Dots and Magnetic Nanoparticles. Cardiovascular Development 2020, 2135, 199 -212.

AMA Style

Galina Nifontova, Fernanda Ramos-Gomes, Frauke Alves, Igor Nabiev, Alyona Sukhanova. Stimulus-Sensitive Theranostic Delivery Systems Based on Microcapsules Encoded with Quantum Dots and Magnetic Nanoparticles. Cardiovascular Development. 2020; 2135 ():199-212.

Chicago/Turabian Style

Galina Nifontova; Fernanda Ramos-Gomes; Frauke Alves; Igor Nabiev; Alyona Sukhanova. 2020. "Stimulus-Sensitive Theranostic Delivery Systems Based on Microcapsules Encoded with Quantum Dots and Magnetic Nanoparticles." Cardiovascular Development 2135, no. : 199-212.

Book chapter
Published: 04 April 2020 in Cardiovascular Development
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Antibody microarrays have become a powerful tool in multiplexed immunoassay technologies. The advantage of microarray technology is the possibility of rapid analysis of multiple targets in a single sample with a high sensitivity, which makes them ideal for high throughput screening. Usually these microarrays contain biological recognition molecules, such as full-size antibodies, antigen-binding fragments, and single-domain antibodies, and a label for detection. Organic fluorophores are the most popular labels, but they suffer from low sensitivity and instability due to their photodegradation. Here, we describe a protocol for fabricating an antibody microarray with highly fluorescent semiconductor nanocrystals or quantum dots (QDs) as the source of fluorescent signals, which may significantly improve the properties of microarrays, including their sensitivity and specificity. Our approach to analyte detection is based on the use of sandwich approach with streptavidin-biotin to assess and monitor the fluorescence signal instead of direct labeling of samples, which helps improve the reproducibility of results and sensitivity of the microarrays. The antibody microarray developed has been tested for its capacity of detecting DNA-PKcs in glial cell lines and measuring cell protein phosphorylation changes caused by camptothecin-induced DNA damage with different protein kinase inhibitors in HeLa cells.

ACS Style

Nizar Ayadi; Florian Lafont; Cathy Charlier; Houda Benhelli-Mokrani; Pavel Sokolov; Alyona Sukhanova; Fabrice Fleury; Igor Nabiev. Comparative Advantages and Limitations of Quantum Dots in Protein Array Applications. Cardiovascular Development 2020, 2135, 259 -273.

AMA Style

Nizar Ayadi, Florian Lafont, Cathy Charlier, Houda Benhelli-Mokrani, Pavel Sokolov, Alyona Sukhanova, Fabrice Fleury, Igor Nabiev. Comparative Advantages and Limitations of Quantum Dots in Protein Array Applications. Cardiovascular Development. 2020; 2135 ():259-273.

Chicago/Turabian Style

Nizar Ayadi; Florian Lafont; Cathy Charlier; Houda Benhelli-Mokrani; Pavel Sokolov; Alyona Sukhanova; Fabrice Fleury; Igor Nabiev. 2020. "Comparative Advantages and Limitations of Quantum Dots in Protein Array Applications." Cardiovascular Development 2135, no. : 259-273.

Journal article
Published: 20 January 2020 in Scientific Reports
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Semiconductor quantum dots (QDs) embedded into polymer microbeads are known to be very attractive emitters for spectral multiplexing and colour encoding. Their luminescence lifetimes or decay kinetics have been, however, rarely exploited as encoding parameter, although they cover time ranges which are not easily accessible with other luminophores. We demonstrate here the potential of QDs made from II/VI semiconductors with luminescence lifetimes of several 10 ns to expand the lifetime range of organic encoding luminophores in multiplexing applications using time-resolved flow cytometry (LT-FCM). For this purpose, two different types of QD-loaded beads were prepared and characterized by photoluminescence measurements on the ensemble level and by single-particle confocal laser scanning microscopy. Subsequently, these lifetime-encoded microbeads were combined with dye-encoded microparticles in systematic studies to demonstrate the potential of these QDs to increase the number of lifetime codes for lifetime multiplexing and combined multiplexing in the time and colour domain (tempo-spectral multiplexing). These studies were done with a recently developed novel luminescence lifetime flow cytometer (LT-FCM setup) operating in the time-domain, that presents an alternative to reports on phase-sensitive lifetime detection in flow cytometry.

ACS Style

Daniel Kage; Katrin Hoffmann; Galina Nifontova; Victor Krivenkov; Alyona Sukhanova; Igor Nabiev; Ute Resch-Genger. Tempo-spectral multiplexing in flow cytometry with lifetime detection using QD-encoded polymer beads. Scientific Reports 2020, 10, 1 -11.

AMA Style

Daniel Kage, Katrin Hoffmann, Galina Nifontova, Victor Krivenkov, Alyona Sukhanova, Igor Nabiev, Ute Resch-Genger. Tempo-spectral multiplexing in flow cytometry with lifetime detection using QD-encoded polymer beads. Scientific Reports. 2020; 10 (1):1-11.

Chicago/Turabian Style

Daniel Kage; Katrin Hoffmann; Galina Nifontova; Victor Krivenkov; Alyona Sukhanova; Igor Nabiev; Ute Resch-Genger. 2020. "Tempo-spectral multiplexing in flow cytometry with lifetime detection using QD-encoded polymer beads." Scientific Reports 10, no. 1: 1-11.

Journal article
Published: 08 November 2019 in Photonics
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Fluorescent semiconductor nanocrystals or quantum dots (QDs) are characterized by unique optical properties, including a high photostability, wide absorption spectrum, and narrow, symmetric fluorescence spectrum. This makes them attractive fluorescent nanolabels for the optical encoding of microcarriers intended for targeted drug delivery, diagnosis, and imaging of transport processes on the body, cellular, and subcellular levels. Incorporation of QDs into carriers in the form of polyelectrolyte microcapsules through layer-by-layer adsorption of oppositely charged polyelectrolyte polymers yields microcapsules with a bright fluorescence signal and adaptable size, structure, and surface characteristics without using organic solvents. The easily modifiable surface of the microcapsules allows for its subsequent functionalization with capture molecules, such as antibodies, which ensures specific and selective interaction with cells, including tumor cells, with the use of the bioconjugation technique developed here. We obtained stable water-soluble nanolabels based on QDs whose surface was modified with polyethylene glycol (PEG) derivatives and determined their colloidal and optical characteristics. The obtained nanocrystals were used to encode polyelectrolyte microcapsules optically. The microcapsule surface was modified with humanized monoclonal antibodies (Abs) recognizing a cancer marker, epidermal growth factor receptor (EGFR). The possibility of effective, specific, and selective delivery of the microcapsules to tumor cells expressing EGFR has been demonstrated. The results show that the QD-encoded polyelectrolyte microcapsules functionalized with monoclonal Abs against EGFR can be used for targeted imaging and diagnosis.

ACS Style

Galina Nifontova; Daria Kalenichenko; Maria Baryshnikova; Fernanda Ramos Gomes; Frauke Alves; Alexander Karaulov; Igor Nabiev; Alyona Sukhanova. Biofunctionalized Polyelectrolyte Microcapsules Encoded with Fluorescent Semiconductor Nanocrystals for Highly Specific Targeting and Imaging of Cancer Cells. Photonics 2019, 6, 117 .

AMA Style

Galina Nifontova, Daria Kalenichenko, Maria Baryshnikova, Fernanda Ramos Gomes, Frauke Alves, Alexander Karaulov, Igor Nabiev, Alyona Sukhanova. Biofunctionalized Polyelectrolyte Microcapsules Encoded with Fluorescent Semiconductor Nanocrystals for Highly Specific Targeting and Imaging of Cancer Cells. Photonics. 2019; 6 (4):117.

Chicago/Turabian Style

Galina Nifontova; Daria Kalenichenko; Maria Baryshnikova; Fernanda Ramos Gomes; Frauke Alves; Alexander Karaulov; Igor Nabiev; Alyona Sukhanova. 2019. "Biofunctionalized Polyelectrolyte Microcapsules Encoded with Fluorescent Semiconductor Nanocrystals for Highly Specific Targeting and Imaging of Cancer Cells." Photonics 6, no. 4: 117.

Conference paper
Published: 03 October 2019 in Fourth International Conference on Applications of Optics and Photonics
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Quantum dots (QDs) are fluorescent semiconductor nanocrystals with a high photostability, wide absorption spectra, and narrow, size-tunable emission spectra, which make them promising nanolabels to be encapsulated in microcarriers used as bioimaging and theranostic tools. Here, we describe an approach to the optical encoding of polyelectrolyte microcapsules with the prepared stable water-soluble QDs and key stages of surface functionalization of these microcapsules with cetuximab, humanised monoclonal anticancer antibody. Obtained conjugates demonstrate the specificity and efficiency of the engineered system as a cancer cell–targeted tracing tool that could be used for cancer diagnosis, treatment and monitoring of cancer therapy.

ACS Style

Galina Nifontova; Maria Baryshnikova; Fernanda Ramos-Gomes; Frauke Alves; Igor Nabiev; Alyona Sukhanova. Engineering of fluorescent biomaging tools for cancer cell targeting based on polyelectrolyte microcapsules encoded with quantum dots. Fourth International Conference on Applications of Optics and Photonics 2019, 11207, 112072D .

AMA Style

Galina Nifontova, Maria Baryshnikova, Fernanda Ramos-Gomes, Frauke Alves, Igor Nabiev, Alyona Sukhanova. Engineering of fluorescent biomaging tools for cancer cell targeting based on polyelectrolyte microcapsules encoded with quantum dots. Fourth International Conference on Applications of Optics and Photonics. 2019; 11207 ():112072D.

Chicago/Turabian Style

Galina Nifontova; Maria Baryshnikova; Fernanda Ramos-Gomes; Frauke Alves; Igor Nabiev; Alyona Sukhanova. 2019. "Engineering of fluorescent biomaging tools for cancer cell targeting based on polyelectrolyte microcapsules encoded with quantum dots." Fourth International Conference on Applications of Optics and Photonics 11207, no. : 112072D.

Conference paper
Published: 03 October 2019 in Fourth International Conference on Applications of Optics and Photonics
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Semiconductor quantum dots (QDs) are characterized by orders of magnitude higher multiphoton linear absorption cross-sections compared with conventional organic dyes. Combined with the QD photoluminescence quantum yield approaching 100%, this fact opens great prospects for the twophoton functional tumor imaging with QDs tagged with highly specific recognition molecules. Single-domain antibodies (sdAbs) or “nanobodies” derived from lamas are the smallest high-affinity recognition molecules, which may be tagged with the QDs thus permitting not only solid tumors multiphoton imaging but also rare disseminated cancer cells and micrometastases in the depth of the tissue to be detected. Additionally, unique photostability of QDs enables signal accumulation and significant enhancement of the sensitivity of routine biochemical and immunohistochemical assays to be obtained when the conjugates of QDs, instead of organic dyes, are used.

ACS Style

Alyona Sukhanova; Fernanda Ramos-Gomes; Patrick Chames; Daniel Baty; Frauke Alves; Pavel Samokhvalov; Igor R. Nabiev. Nanophotonic tools based on the conjugates of nanoparticles with the single-domain antibodies for multi-photon micrometastases detection and ultrasensitive biochemical assays. Fourth International Conference on Applications of Optics and Photonics 2019, 11207, 112072A .

AMA Style

Alyona Sukhanova, Fernanda Ramos-Gomes, Patrick Chames, Daniel Baty, Frauke Alves, Pavel Samokhvalov, Igor R. Nabiev. Nanophotonic tools based on the conjugates of nanoparticles with the single-domain antibodies for multi-photon micrometastases detection and ultrasensitive biochemical assays. Fourth International Conference on Applications of Optics and Photonics. 2019; 11207 ():112072A.

Chicago/Turabian Style

Alyona Sukhanova; Fernanda Ramos-Gomes; Patrick Chames; Daniel Baty; Frauke Alves; Pavel Samokhvalov; Igor R. Nabiev. 2019. "Nanophotonic tools based on the conjugates of nanoparticles with the single-domain antibodies for multi-photon micrometastases detection and ultrasensitive biochemical assays." Fourth International Conference on Applications of Optics and Photonics 11207, no. : 112072A.

Journal article
Published: 01 October 2019 in Bulletin of the Lebedev Physics Institute
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Using fluorescence resonance energy transfer, the physical interaction of nanoparticles with biopolymers is studied by the example of the interaction of quantum dots (energy donor) with two-chain oligonucleotides labeled with a fluorescent organic dye BDP TR (energy acceptor). The high-efficiency resonance energy transfer from quantum dots to an organic dye not only makes it possible to study the dependence of the transfer efficiency on the donor-acceptor molar ratio but also represents an efficient tool for studying the interactions of oligonucleotides of different structures with nanoscale objects.

ACS Style

A. V. Kosmyntseva; Irina Petrova; Yu. P. Rakovich; Alyona Sukhanova; I. R. Nabiev. Physical Interactions of Biopolymers with Nanoparticles. Bulletin of the Lebedev Physics Institute 2019, 46, 306 -308.

AMA Style

A. V. Kosmyntseva, Irina Petrova, Yu. P. Rakovich, Alyona Sukhanova, I. R. Nabiev. Physical Interactions of Biopolymers with Nanoparticles. Bulletin of the Lebedev Physics Institute. 2019; 46 (10):306-308.

Chicago/Turabian Style

A. V. Kosmyntseva; Irina Petrova; Yu. P. Rakovich; Alyona Sukhanova; I. R. Nabiev. 2019. "Physical Interactions of Biopolymers with Nanoparticles." Bulletin of the Lebedev Physics Institute 46, no. 10: 306-308.

Original research article
Published: 30 July 2019 in Frontiers in Chemistry
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Nanoparticles attract much interest as fluorescent labels for diagnostic and therapeutic tools, although their applications are often hindered by size- and shape-dependent cytotoxicity. This cytotoxicity is related not only to the leak of toxic metals from nanoparticles into a biological solution, but also to molecular cytotoxicity effects determined by the formation of a protein corona, appearance of an altered protein conformation leading to exposure of cryptic epitopes and cooperative effects involved in the interaction of proteins and peptides with nanoparticles. In the last case, nanoparticles may serve, depending on their nature, as centers of self-association or fibrillation of proteins and peptides, provoking amyloid-like proteinopathies, or as inhibitors of self-association of proteins, or they can self-assemble on biopolymers as on templates. In this study, human insulin protein was used to analyze nanoparticle-induced proteinopathy in physiological conditions. It is known that human insulin may form amyloid fibers, but only under extreme experimental conditions (very low pH and high temperatures). Here, we have shown that the quantum dots (QDs) may induce amyloid-like fibrillation of human insulin under physiological conditions through a complex process strongly dependent on the size and surface charge of QDs. The insulin molecular structure and fibril morphology have been shown to be modified at different stages of its fibrillation, which has been proved by comparative analysis of the data obtained using circular dichroism, dynamic light scattering, amyloid-specific thioflavin T (ThT) assay, transmission electron microscopy, and high-speed atomic force microscopy. We have found important roles of the QD size and surface charge in the destabilization of the insulin structure and the subsequent fibrillation. Remodeling of the insulin secondary structure accompanied by remarkable increase in the rate of formation of amyloid-like fibrils under physiologically normal conditions was observed when the protein was incubated with QDs of exact specific diameter coated with slightly negative specific polyethylene glycol (PEG) derivatives. Strongly negatively or slightly positively charged PEG-modified QDs of the same specific diameter or QDs of bigger or smaller diameters had no effect on insulin fibrillation. The observed effects pave the way to the control of amyloidosis proteinopathy by varying the nanoparticle size and surface charge.

ACS Style

Alyona Sukhanova; Simon Poly; Svetlana Bozrova; Éléonore Lambert; Maxime Ewald; Alexander Karaulov; Michael Molinari; Igor Nabiev. Nanoparticles With a Specific Size and Surface Charge Promote Disruption of the Secondary Structure and Amyloid-Like Fibrillation of Human Insulin Under Physiological Conditions. Frontiers in Chemistry 2019, 7, 480 .

AMA Style

Alyona Sukhanova, Simon Poly, Svetlana Bozrova, Éléonore Lambert, Maxime Ewald, Alexander Karaulov, Michael Molinari, Igor Nabiev. Nanoparticles With a Specific Size and Surface Charge Promote Disruption of the Secondary Structure and Amyloid-Like Fibrillation of Human Insulin Under Physiological Conditions. Frontiers in Chemistry. 2019; 7 ():480.

Chicago/Turabian Style

Alyona Sukhanova; Simon Poly; Svetlana Bozrova; Éléonore Lambert; Maxime Ewald; Alexander Karaulov; Michael Molinari; Igor Nabiev. 2019. "Nanoparticles With a Specific Size and Surface Charge Promote Disruption of the Secondary Structure and Amyloid-Like Fibrillation of Human Insulin Under Physiological Conditions." Frontiers in Chemistry 7, no. : 480.

Journal article
Published: 19 June 2019 in Scientific Reports
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Circulating cancer markers are metabolic products found in body fluids of cancer patients, which are specific for a certain type of malignant tumors. Cancer marker detection plays a key role in cancer diagnosis, treatment, and disease monitoring. The growing need for early cancer diagnosis requires quick and sensitive analytical approaches to detection of cancer markers. The approach based on the photonic crystal surface mode (PC SM) detection has been developed as a label-free high-precision biosensing technique. It allows real-time monitoring of molecular and cellular interactions using independent recording of the total internal reflection angle and the excitation angle of the PC surface wave. We used the PC SM technique for simultaneous detection of the ovarian cancer marker cancer antigen 125 and two breast cancer markers, human epidermal growth factor receptor 2 and cancer antigen 15-3. The new assay is based on the real-time flow detection of specific interaction between the antigens and capture antibodies. Its particular advantage is the possibility of multichannel recording with the same chip, which can be used for multiplexed detection of several cancer markers in a single experiment. The developed approach demonstrates high specificity and sensitivity for detection of all three biomarkers.

ACS Style

Irina Petrova; Valery Konopsky; Igor Nabiev; Alyona Sukhanova. Label-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection. Scientific Reports 2019, 9, 1 -9.

AMA Style

Irina Petrova, Valery Konopsky, Igor Nabiev, Alyona Sukhanova. Label-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection. Scientific Reports. 2019; 9 (1):1-9.

Chicago/Turabian Style

Irina Petrova; Valery Konopsky; Igor Nabiev; Alyona Sukhanova. 2019. "Label-Free Flow Multiplex Biosensing via Photonic Crystal Surface Mode Detection." Scientific Reports 9, no. 1: 1-9.

Short communication
Published: 24 April 2019 in Materials Science and Engineering: C
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Graphene is one of the crystalline forms of carbon, along with diamond, graphite, carbon nanotubes, and fullerenes, and is considered as a revolutionary and innovating product. The use of a graphene-based nanolabels is one of the latest and most prominent application of graphene, especially in the field of diagnosis and, recently, in loco radiotherapy when coupled with radioisotopes. However, its biological behavior and mutagenicity in different cell or animal models, as well as the in vivo functional activities, are still unrevealed. In this study we have developed by a green route of synthesizing graphene quantum dots (GQDs) and characterized them. We have also developed a methodology for direct radiolabeling of GQDs with radioisotopes.Finally; we have evaluated in vivo biological behavior of GQDs using two different mice models and tested in vitro mutagenicity of GQDs. The results have shown that GQDs were formed with a size range of 160-280 nm, which was confirmed by DRX and Raman spectroscopy analysis, corroborating that the green synthesis is an alternative, environmentally friendly way to produce graphene. The radiolabeling test has shown that stable radiolabeled GQDs can be produced with a high yield (>90%). The in vivo test has demonstrated a ubiquitous behavior when administered to healthy animals, with a high uptake by liver (>26%) and small intestine (>25%). Otherwise, in an inflammation/VEGF hyperexpression animal model (endometriosis), a very peculiar behavior of GQDs was observed, with a high uptake by kidneys (over 85%). The mutagenicity test has demonstrated A:T to G:C substitutions suggesting that GQDs exhibits mutagenic activity.

ACS Style

Frederico Duarte de Menezes; Sara Rhaissa Rezende dos Reis; Suyene Rocha Pinto; Filipe Leal Portilho; Francisco Do Vale Chaves e Mello; Edward Helal-Neto; Aline Oliveira Da Silva De Barros; Luciana Magalhães Rebêlo Alencar; Alan Silva de Menezes; Clenilton Santos; Aldilene Saraiva-Souza; Jamila Alessandra Perini; Daniel Escorsim Machado; Israel Felzenswalb; Carlos Fernando Araujo-Lima; Alyona Sukhanova; Igor Nabiev; Ralph Santos-Oliveira. Graphene quantum dots unraveling: Green synthesis, characterization, radiolabeling with 99mTc, in vivo behavior and mutagenicity. Materials Science and Engineering: C 2019, 102, 405 -414.

AMA Style

Frederico Duarte de Menezes, Sara Rhaissa Rezende dos Reis, Suyene Rocha Pinto, Filipe Leal Portilho, Francisco Do Vale Chaves e Mello, Edward Helal-Neto, Aline Oliveira Da Silva De Barros, Luciana Magalhães Rebêlo Alencar, Alan Silva de Menezes, Clenilton Santos, Aldilene Saraiva-Souza, Jamila Alessandra Perini, Daniel Escorsim Machado, Israel Felzenswalb, Carlos Fernando Araujo-Lima, Alyona Sukhanova, Igor Nabiev, Ralph Santos-Oliveira. Graphene quantum dots unraveling: Green synthesis, characterization, radiolabeling with 99mTc, in vivo behavior and mutagenicity. Materials Science and Engineering: C. 2019; 102 ():405-414.

Chicago/Turabian Style

Frederico Duarte de Menezes; Sara Rhaissa Rezende dos Reis; Suyene Rocha Pinto; Filipe Leal Portilho; Francisco Do Vale Chaves e Mello; Edward Helal-Neto; Aline Oliveira Da Silva De Barros; Luciana Magalhães Rebêlo Alencar; Alan Silva de Menezes; Clenilton Santos; Aldilene Saraiva-Souza; Jamila Alessandra Perini; Daniel Escorsim Machado; Israel Felzenswalb; Carlos Fernando Araujo-Lima; Alyona Sukhanova; Igor Nabiev; Ralph Santos-Oliveira. 2019. "Graphene quantum dots unraveling: Green synthesis, characterization, radiolabeling with 99mTc, in vivo behavior and mutagenicity." Materials Science and Engineering: C 102, no. : 405-414.

Journal article
Published: 01 April 2019 in Technical Physics Letters
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Plasmonic nanoparticles have become a popularly accepted research tool in optoelectronics, photonics, and biomedical applications. The relatively recently appearing semiconductor plasmonic nanoparticles, as opposed to metal ones, are characterized by infrared plasmonic optical transitions and their application has a great future. In this work, the possibility of conversion of semiconductor (excitonic) fluorescence nanocrystals, i.e., quantum dots of the CuInS2 composition, to plasmonic nanoparticles by postsynthetic treatment without changes in the chemical composition of inorganic part of the nanocrystals was demonstrated for the first time ever.

ACS Style

P. S. Samokhvalov; D. O. Volodin; S. V. Bozrova; D. S. Dovzhenko; M. A. Zvaigzne; P. A. Lin’Kov; G. O. Nifontova; Irina Petrova; Alyona Sukhanova; I. R. Nabiev. Conversion of Semiconductor Nanoparticles to Plasmonic Materials by Targeted Substitution of Surface-Bound Organic Ligands. Technical Physics Letters 2019, 45, 317 -320.

AMA Style

P. S. Samokhvalov, D. O. Volodin, S. V. Bozrova, D. S. Dovzhenko, M. A. Zvaigzne, P. A. Lin’Kov, G. O. Nifontova, Irina Petrova, Alyona Sukhanova, I. R. Nabiev. Conversion of Semiconductor Nanoparticles to Plasmonic Materials by Targeted Substitution of Surface-Bound Organic Ligands. Technical Physics Letters. 2019; 45 (4):317-320.

Chicago/Turabian Style

P. S. Samokhvalov; D. O. Volodin; S. V. Bozrova; D. S. Dovzhenko; M. A. Zvaigzne; P. A. Lin’Kov; G. O. Nifontova; Irina Petrova; Alyona Sukhanova; I. R. Nabiev. 2019. "Conversion of Semiconductor Nanoparticles to Plasmonic Materials by Targeted Substitution of Surface-Bound Organic Ligands." Technical Physics Letters 45, no. 4: 317-320.

Original research article
Published: 30 January 2019 in Frontiers in Chemistry
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Imaging agents and drug carriers are commonly targeted toward cancer cell through functionalization with specific recognition molecules. Quantum dots (QDs) are fluorescent semiconductor nanocrystals whose extraordinary brightness and photostability make them attractive for direct fluorescent labeling of biomolecules or optical encoding of the membranes and cells. Here, we analyse the cytotoxicity of QD-encoded microcapsules, validate an approach to the activation of the microcapsule's surface for further functionalization with monoclonal antibody Trastuzumab, a humanized monoclonal antibody targeting the extracellular domain of the human epidermal growth factor receptor 2 (HER2) and already in clinical use for the treatment of HER2 positive breast cancer. In addition, we characterize the cell-specific targeting activity of the resultant bio-conjugate by immunofluorescence assay (IFA) and real-time analysis of interaction of the conjugates with live HER2 overexpressing human breast cancer cells. We demonstrate, that encapsulation of QDs into the polymer shell using the layer-by-layer deposition method yields highly fluorescent polyelectrolyte microcapsules with a homogeneous size distribution and biocompatibility upon in vitro treatment of cancer cells. Carbodiimide surface activation ensures optimal disperse and optical characteristics of the QD-encoded microcapsules before antibody conjugation. The prepared conjugates of the microcapsules with cancer-specific monoclonal antibody targeting HER2 provide sufficiently sensitive and specific antibody-mediated binding of the microcapsules with live cancer cells, which demonstrated their potential as prospective cancer cell–targeting agents.

ACS Style

Galina Nifontova; Fernanda Ramos-Gomes; Maria Baryshnikova; Frauke Alves; Igor Nabiev; Alyona Sukhanova. Cancer Cell Targeting With Functionalized Quantum Dot-Encoded Polyelectrolyte Microcapsules. Frontiers in Chemistry 2019, 7, 1 .

AMA Style

Galina Nifontova, Fernanda Ramos-Gomes, Maria Baryshnikova, Frauke Alves, Igor Nabiev, Alyona Sukhanova. Cancer Cell Targeting With Functionalized Quantum Dot-Encoded Polyelectrolyte Microcapsules. Frontiers in Chemistry. 2019; 7 ():1.

Chicago/Turabian Style

Galina Nifontova; Fernanda Ramos-Gomes; Maria Baryshnikova; Frauke Alves; Igor Nabiev; Alyona Sukhanova. 2019. "Cancer Cell Targeting With Functionalized Quantum Dot-Encoded Polyelectrolyte Microcapsules." Frontiers in Chemistry 7, no. : 1.

Nano express
Published: 18 January 2019 in Nanoscale Research Letters
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Fluorescent imaging is a widely used technique for detecting and monitoring the distribution, interaction, and transformation processes at molecular, cellular, and tissue level in modern diagnostic and other biomedical applications. Unique photophysical properties of fluorescent semiconductor nanocrystals “quantum dots” (QDs) make them advanced fluorophores for fluorescent labeling of biomolecules or optical encoding of microparticles to be used as bioimaging and theranostic agents in targeted delivery, visualization, diagnostics, and imaging. This paper reports on the results of development of an improved approach to the optical encoding of polyelectrolyte microcapsules with stable, covered with the multifunctional polyethyleneglycol derivatives water-soluble QDs, as well as characterization of the optical properties, morphological and structural properties of the encoded microcapsules. The embedding of QDs into the polymer microcapsule membrane through layer-by-layer deposition on a preliminarily formed polymeric polyelectrolyte shell makes it possible to obtain bright fluorescent particles with an adapted charge and size distribution that are distinctly discernible by flow cytometry as individual homogeneous populations. The fluorescent microcapsules developed can be used in further designing bioimaging and theranostic agents sensitive to various external stimuli along with photoexcitation.

ACS Style

Galina Nifontova; Anton Efimov; Olga Agapova; Igor Agapov; Igor Nabiev; Alyona Sukhanova. Bioimaging Tools Based on Polyelectrolyte Microcapsules Encoded with Fluorescent Semiconductor Nanoparticles: Design and Characterization of the Fluorescent Properties. Nanoscale Research Letters 2019, 14, 29 .

AMA Style

Galina Nifontova, Anton Efimov, Olga Agapova, Igor Agapov, Igor Nabiev, Alyona Sukhanova. Bioimaging Tools Based on Polyelectrolyte Microcapsules Encoded with Fluorescent Semiconductor Nanoparticles: Design and Characterization of the Fluorescent Properties. Nanoscale Research Letters. 2019; 14 (1):29.

Chicago/Turabian Style

Galina Nifontova; Anton Efimov; Olga Agapova; Igor Agapov; Igor Nabiev; Alyona Sukhanova. 2019. "Bioimaging Tools Based on Polyelectrolyte Microcapsules Encoded with Fluorescent Semiconductor Nanoparticles: Design and Characterization of the Fluorescent Properties." Nanoscale Research Letters 14, no. 1: 29.

Journal article
Published: 01 November 2018 in Optics and Spectroscopy
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Semiconductor quantum dots (QDs) are characterized by orders of magnitude higher multiphoton linear absorption cross-sections compared with conventional organic dyes. Combined with the QD photoluminescence quantum yield approaching 100% and their rock-solid photostability, this fact opens great prospects for the two-photon functional tumor imaging with QDs tagged with highly specific recognition molecules. Single-domain antibodies (sdAbs) or “nanobodies” derived from lamas are the smallest high-affinity recognition molecules, which may be tagged with the QDs thus permitting not only solid tumors multiphoton imaging but also rare disseminated cancer cells and micrometastases in the depth of the tissue to be detected.

ACS Style

Alyona Sukhanova; F. Ramos-Gomes; F. Alves; P. Chames; D. Baty; I. Nabiev. Advanced Nanotools for Imaging of Solid Tumors and Circulating and Disseminated Cancer Cells. Optics and Spectroscopy 2018, 125, 703 -707.

AMA Style

Alyona Sukhanova, F. Ramos-Gomes, F. Alves, P. Chames, D. Baty, I. Nabiev. Advanced Nanotools for Imaging of Solid Tumors and Circulating and Disseminated Cancer Cells. Optics and Spectroscopy. 2018; 125 (5):703-707.

Chicago/Turabian Style

Alyona Sukhanova; F. Ramos-Gomes; F. Alves; P. Chames; D. Baty; I. Nabiev. 2018. "Advanced Nanotools for Imaging of Solid Tumors and Circulating and Disseminated Cancer Cells." Optics and Spectroscopy 125, no. 5: 703-707.

Journal article
Published: 29 September 2018 in Bulletin of Russian State Medical University
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Conventional techniques for food and water quality control and environmental monitoring in general have a number of drawbacks. Below we propose a label-free highly accurate analytical technique for multiplex detection of biomarkers based on the analysis of propagation of Bloch waves on the surface of a photonic crystal. The technique can be used to measure molecular and cell affinity interactions in real time by recording critical and excitation angles of the surface wave on the surface of a photonic crystal. Based on the analysis of photonic crystal surface modes, we elaborated a protocol for the detection of the exotoxin A of Pseudomonas aeruginosa and the heat-labile toxin LT of Escherichia coli. The protocol exploits detection of affinity interactions between antigens pumped through a microfluidic cell and detector antibodies conjugated to the chemically activated silica chip. The proposed technique is highly sensitive, cheap and less time-consuming in comparison with surface plasmon resonance.

ACS Style

I. O. Petrova; V. N. Konopsky; A. V. Sukhanova; I. R. Nabiev. Multiparametric detection of bacterial contamination based on the photonic crystal surface mode detection. Bulletin of Russian State Medical University 2018, 19 -24.

AMA Style

I. O. Petrova, V. N. Konopsky, A. V. Sukhanova, I. R. Nabiev. Multiparametric detection of bacterial contamination based on the photonic crystal surface mode detection. Bulletin of Russian State Medical University. 2018; ((4)2018):19-24.

Chicago/Turabian Style

I. O. Petrova; V. N. Konopsky; A. V. Sukhanova; I. R. Nabiev. 2018. "Multiparametric detection of bacterial contamination based on the photonic crystal surface mode detection." Bulletin of Russian State Medical University , no. (4)2018: 19-24.

Conference paper
Published: 01 June 2018 in 2018 International Conference Laser Optics (ICLO)
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Early detection of the micrometastases is still a challenge. Here, we demonstrate the use of nanoprobes engineered from the single-domain antibodies and fluorescent quantum dots for single- and two-photon detection and imaging of human micrometastases in ex vivo biological samples of breast and pancreatic metastatic tumour models and analyze the nanotoxicity issues related to their potential in vivo applications.

ACS Style

Alyona Sukhanova; P. Chames; D. Baty; F. Ramos-Gomes; F. Alves; I. Nabiev. Nanophotonic Functional Imaging and Related Nanotoxicity Issues. 2018 International Conference Laser Optics (ICLO) 2018, 531 -531.

AMA Style

Alyona Sukhanova, P. Chames, D. Baty, F. Ramos-Gomes, F. Alves, I. Nabiev. Nanophotonic Functional Imaging and Related Nanotoxicity Issues. 2018 International Conference Laser Optics (ICLO). 2018; ():531-531.

Chicago/Turabian Style

Alyona Sukhanova; P. Chames; D. Baty; F. Ramos-Gomes; F. Alves; I. Nabiev. 2018. "Nanophotonic Functional Imaging and Related Nanotoxicity Issues." 2018 International Conference Laser Optics (ICLO) , no. : 531-531.

Journal article
Published: 15 March 2018 in Scientific Reports
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Early detection of malignant tumours and, especially, micrometastases and disseminated tumour cells is still a challenge. In order to implement highly sensitive diagnostic tools we demonstrate the use of nanoprobes engineered from nanobodies (single-domain antibodies, sdAbs) and fluorescent quantum dots (QDs) for single- and two-photon detection and imaging of human micrometastases and disseminated tumour cells in ex vivo biological samples of breast and pancreatic metastatic tumour mouse models expressing human epidermal growth factor receptor 2 (HER2) or carcinoembryonic antigen (CEA). By staining thin (5–10 µm) paraffin and thick (50 µm) agarose tissue sections, we detected HER2- and CEA-positive human tumour cells infiltrating the surrounding tissues or metastasizing to different organs, including the brain, testis, lung, liver, and lymph nodes. Compared to conventional fluorescently labelled antibodies the sdAb-HER2-QD and sdAb-CEA-QD nanoprobes are superior in detecting micrometastases in tissue sections by lower photobleaching and higher brightness of fluorescence signals ensuring much better discrimination of positive signals versus background. Very high two-photon absorption cross-sections of QDs and small size of the nanoprobes ensure efficient imaging of thick tissue sections unattainable with conventional fluorescent probes. The nanobody–QD probes will help to improve early cancer diagnosis and prognosis of progression by assessing metastasis.

ACS Style

Fernanda Ramos-Gomes; Julia Bode; Alyona Sukhanova; Svetlana V. Bozrova; Mara Saccomano; Miso Mitkovski; Julia Eva Krueger; Anja K. Wege; Walter Stuehmer; Pavel S. Samokhvalov; Daniel Baty; Patrick Chames; Igor Nabiev; Frauke Alves. Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots. Scientific Reports 2018, 8, 1 -12.

AMA Style

Fernanda Ramos-Gomes, Julia Bode, Alyona Sukhanova, Svetlana V. Bozrova, Mara Saccomano, Miso Mitkovski, Julia Eva Krueger, Anja K. Wege, Walter Stuehmer, Pavel S. Samokhvalov, Daniel Baty, Patrick Chames, Igor Nabiev, Frauke Alves. Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots. Scientific Reports. 2018; 8 (1):1-12.

Chicago/Turabian Style

Fernanda Ramos-Gomes; Julia Bode; Alyona Sukhanova; Svetlana V. Bozrova; Mara Saccomano; Miso Mitkovski; Julia Eva Krueger; Anja K. Wege; Walter Stuehmer; Pavel S. Samokhvalov; Daniel Baty; Patrick Chames; Igor Nabiev; Frauke Alves. 2018. "Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots." Scientific Reports 8, no. 1: 1-12.

Nano review
Published: 07 February 2018 in Nanoscale Research Letters
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Studies on the methods of nanoparticle (NP) synthesis, analysis of their characteristics, and exploration of new fields of their applications are at the forefront of modern nanotechnology. The possibility of engineering water-soluble NPs has paved the way to their use in various basic and applied biomedical researches. At present, NPs are used in diagnosis for imaging of numerous molecular markers of genetic and autoimmune diseases, malignant tumors, and many other disorders. NPs are also used for targeted delivery of drugs to tissues and organs, with controllable parameters of drug release and accumulation. In addition, there are examples of the use of NPs as active components, e.g., photosensitizers in photodynamic therapy and in hyperthermic tumor destruction through NP incorporation and heating. However, a high toxicity of NPs for living organisms is a strong limiting factor that hinders their use in vivo. Current studies on toxic effects of NPs aimed at identifying the targets and mechanisms of their harmful effects are carried out in cell culture models; studies on the patterns of NP transport, accumulation, degradation, and elimination, in animal models. This review systematizes and summarizes available data on how the mechanisms of NP toxicity for living systems are related to their physical and chemical properties.

ACS Style

Alyona Sukhanova; Svetlana Bozrova; Pavel Sokolov; Mikhail Berestovoy; Alexander Karaulov; Igor Nabiev. Dependence of Nanoparticle Toxicity on Their Physical and Chemical Properties. Nanoscale Research Letters 2018, 13, 1 -21.

AMA Style

Alyona Sukhanova, Svetlana Bozrova, Pavel Sokolov, Mikhail Berestovoy, Alexander Karaulov, Igor Nabiev. Dependence of Nanoparticle Toxicity on Their Physical and Chemical Properties. Nanoscale Research Letters. 2018; 13 (1):1-21.

Chicago/Turabian Style

Alyona Sukhanova; Svetlana Bozrova; Pavel Sokolov; Mikhail Berestovoy; Alexander Karaulov; Igor Nabiev. 2018. "Dependence of Nanoparticle Toxicity on Their Physical and Chemical Properties." Nanoscale Research Letters 13, no. 1: 1-21.