Drought is a major abiotic stress that adversely affects the growth and productivity of plants. Malondialdehyde (MDA), a substance produced by membrane lipids in response to reactive oxygen species (ROS), can be used as a drought indicator to evaluate the degree of plasma membrane damage and the ability of plants to drought stress tolerance. Still measuring MDA is usually a labor- and time-consuming task. In this study, near-infrared (NIR) spectroscopy combined with partial least squares (PLS) was used to obtain rapid and high-throughput measurements of MDA, and the application of this technique to plant drought stress experiments was also investigated. Two exotic conifer tree species, namely, slash pine (Pinus elliottii) and loblolly pine (Pinus taeda), were used as plant material exposed to drought stress; different types of spectral preprocessing methods and important feature-selection algorithms were applied to the PLS model to calibrate it and obtain the best MDA-predicting model. The results show that the best PLS model is established via the combined treatment of detrended variable–significant multivariate correlation algorithm (DET-sMC), where latent variables (LVs) were 6. This model has a respectable predictive capability, with a correlation coefficient (R²) of 0.66, a root mean square error (RMSE) of 2.28%, and a residual prediction deviation (RPD) of 1.51, and it was successfully implemented in drought stress experiments as a reliable and non-destructive method to detect the MDA content in real time.

Background: Karst vegetation is of great significance for ecological restoration in karst areas. Vegetation Indices (VIs) are mainly related to plant yield which is helpful to understand the status of ecological restoration in karst areas. Recently, karst vegetation surveys have gradually shifted from field surveys to remote sensing-based methods. Coupled with the machine learning methods, the Unmanned Aerial Vehicle (UAV) multispectral remote sensing data can effectively improve the detection accuracy of vegetation and extract the important spectrum features.Results: In this study, UAV multispectral image data at flight altitudes of 100 m, 200 m, and 400 m were collected to be applied for vegetation detection in a karst area. The resulting ground resolutions of the 100 m, 200 m, and 400 m data are 5.29, 10.58, and 21.16 cm/pixel, respectively. Four machine learning models, including Random Forest (RF), Support Vector Machine (SVM), Gradient Boosting Machine (GBM), and Deep Learning (DL), were compared to test the performance of vegetation coverage detection. 5 spectral values (Red, Green, Blue, NIR, Red edge) and 16 VIs were selected to perform variable importance analysis on the best detection models. The results show that the best model for each flight altitude has the highest accuracy in detecting its training data (over 90%), and the GBM model constructed based on all data at all flight altitudes yields the best detection performance covering all data, with an overall accuracy of 95.66%. The variables that were significantly correlated and not correlated with the best model were the Modified Soil Adjusted Vegetation Index (MSAVI) and the Modified Anthocyanin Content Index (MACI), respectively. Finally, the best model was used to invert the complete UAV images at different flight altitudes.Conclusions: In general, the GBM_all model constructed based on UAV imaging with all flight altitudes was feasible to accurately detect karst vegetation coverage. The prediction models constructed based on data from different flight altitudes had a certain similarity in the distribution of vegetation index importance. Combined with the method of visual interpretation, the karst green vegetation predicted by the best model was in good agreement with the ground truth, and other land types including hay, rock, and soil were well predicted. This study provided a methodological reference for the detection of karst vegetation coverage in eastern China.

Rapid and accurate plant growth and biomass estimation is essential for formulating and implementing targeted forest cultivation measures. In this study, RGB-D imaging technology was used to obtain the RGB and depth imaging data for a Toona sinensis seedling canopy to estimate plant growth and aboveground biomass (AGB). Three hundred T. sinensis seedlings from 20 varieties were planted under five different drought stress treatments. The U-Net model was applied first to achieve highly accurate segmentation of plants from complex backgrounds. Simple linear regression (SLR) was used for plant height prediction, and the other three models, including multivariate linear (ML), random forest (RF) and multilayer perceptron (MLP) regression, were applied to predict the AGB and compared for optimal model selection. The results showed that the SLR model yields promising and reliable results for the prediction of plant height, with R² and RMSE values of 0.72 and 1.89 cm, respectively. All three regression methods perform well in the prediction of AGB estimation. MLP yields the highest accuracy in predicting dry and fresh aboveground biomass compared to the other two regression models, with R² values of 0.77 and 0.83, respectively. The combination of Gray, Green minus red (GMR) and Excess green index (ExG) was identified as the key predictor by RReliefF for predicting dry AGB. GMR was the most important in predicting fresh AGB. This study demonstrated that the merits of RGB-D and machine learning models are effective phenotyping techniques for plant height and AGB prediction, and can be used to assist dynamic responses to drought stress for breeding selection.