Spiders are a diverse group with a high eco-morphological diversity, which complicates anatomical descriptions especially with regard to its terminology. New terms are constantly proposed, and definitions and limits of anatomical concepts are regularly updated. Therefore, it is often challenging to find the correct terms, even for trained scientists, especially when the terminology has obstacles such as synonyms, disputed definitions, ambiguities, or homonyms. Here, we present the Spider Anatomy Ontology (SPD), which we developed combining the functionality of a glossary (a controlled defined vocabulary) with a network of formalized relations between terms that can be used to compute inferences. The SPD follows the guidelines of the Open Biomedical Ontologies and is available through the NCBO BioPortal (ver. 1.1). It constitutes of 757 valid terms and definitions, is rooted with the Common Anatomy Reference Ontology (CARO), and has cross references to other ontologies, especially of arthropods. The SPD offers a wealth of anatomical knowledge that can be used as a resource for any scientific study as, for example, to link images to phylogenetic datasets, compute structural complexity over phylogenies, and produce ancestral ontologies. By using a common reference in a standardized way, the SPD will help bridge diverse disciplines, such as genomics, taxonomy, systematics, evolution, ecology, and behavior.

Ninetinae is a group of small to tiny short-legged spiders largely restricted to arid habitats. Among daddy-long-legs spiders (Pholcidae) this is by far the least diverse subfamily but this may partly be a result of inadequate collecting, poor representation in collections or scientific neglect. We build on a large recent collection of the ninetine genus Papiamenta Huber, 2000 from the Leeward Antilles and use cytochrome oxidase 1 (COI) sequences, extensive scanning electron microscopy data, transmission electron microscopy data and karyotyping to analyse this geographically isolated and poorly known island genus. COI sequences support the split between the two morphologically distinct species on Curaçao but genetic distances between these are surprisingly low (7.4–9.8%; mean 8.6%). The type species P. levii (Gertsch, 1982) may include more than one species but COI and morphology suggest conflicting clade limits. A third species, P. bonay Huber sp. nov. is newly described from Bonaire. Our data on sperm ultrastructure and karyology are puzzling as these suggest different phylogenetic affinities of Papiamenta to other genera. Males transfer sperm as individual sperm (cleistosperm), agreeing with the putative closest relatives as suggested by molecular data, the North American genera Pholcophora and Tolteca. The sex chromosome system (X1X2X3Y) of P. levii, however, is as in the South American Ninetinae genera Gertschiola and Nerudia but different from the putative closest relatives. ZooBank: urn:lsid:zoobank.org:pub:7A6A2E84-3A61-4637-AF6F-0E31A9FA79A8

Background: Most arthropods pass through several molting stages (instars) before reaching sexual maturity. In spiders, very little is known about the male genital system, its development and seminal secretions. For example, it is unknown whether spermatozoa exist prior to-, or only after the final molt. Likewise, it is unclear whether sperm are produced throughout male adulthood or only once in a lifetime, as is whether seminal secretions contain factors capable of manipulating female behavior. In order to shed light on these aspects of the reproductive biology of spiders, we investigated the male genital system of the common cellar spider Pholcus phalangioides, with special emphasis on its development and seminal secretions. Results: Testes already display all stages of spermatogenesis in subadult males (about four weeks before the final molt). Their vasa deferentia possess proximally a very voluminous lumen containing dense seminal fluid and few spermatozoa, whereas the distal part is seemingly devoid of contents. Spermatoza of P. phalangioides are typical cleistospermia with individual secretion sheaths. In male stages approximately two weeks prior to the final molt, the lumina of the testes are wider and filled with a dense secretion. The wide, proximal portion of the vasa deferentia is filled with secretion and a large number of spermatozoa, and the narrow distal part also contains secretion. In adult males, the wide lumina of the testes are packed with spermatozoa and secretions. The latter are produced by the somatic cells that bear microvilli and contain many vesicles. The lumina of the vasa deferentia are narrow and filled with spermatozoa and secretions. We could identify a dense matrix of secretion consisting of mucosubstances and at least three types of secretion droplets, likely consisting of proteinaceous substances. Conclusion: This study reveals that spermatogenesis begins weeks before maturity and takes place continuously in the long-lived males of P. phalangioides. Possible functions of the various types of secretion in the seminal fluid and previously investigated female secretions are discussed in the light of sexual selection.

Once thought to be energetically cheap and easy to produce, empirical work has shown that sperm is a costly and limited resource for males. In some spider species, there is behavioral evidence that sperm are permanently depleted after a single mating. This extreme degree of mating investment appears to co-occur with other reproductive strategies common to spiders, e.g. genital mutilation and sexual cannibalism. Here we corroborate that sperm depletion in the golden orb-web spider Nephila clavipes is permanent by uncovering its mechanistic basis using light and electron microscopy. In addition, we use a phylogeny-based statistical analysis to test the evolutionary relationships between permanent sperm depletion (PSD) and other reproductive strategies in spiders. Male testes do not produce sperm during adulthood, which is unusual in spiders. Instead, spermatogenesis is nearly synchronous and ends before the maturation molt. Testis size decreases as males approach their maturation molt and reaches its lowest point after sperm is transferred into the male copulatory organs (pedipalps). As a consequence, the amount of sperm available to males for mating is limited to the sperm contained in the pedipalps, and once it is used, males lose their ability to fertilize eggs. Our data suggest that PSD has evolved independently at least three times within web-building spiders and is significantly correlated with the evolution of other mating strategies that limit males to monogamy, including genital mutilation and sexual cannibalism. We conclude that PSD may be an energy-saving adaptation in species where males are limited to monogamy. This could be particularly important in web-building spiders where extreme sexual size dimorphism results in large, sedentary females and small, searching males who rarely feed as adults and are vulnerable to starvation. Future work will explore possible energetic benefits and the evolutionary lability of PSD relative to other mate-limiting reproductive behaviors.