Aboveground biomass is a key indicator of a grassland ecosystem. Accurate estimation from remote sensing is important for understanding the response of grasslands to climate change and disturbance at a large scale. However, the precision of remote sensing inversion is limited by a lack in the ground truth and scale mismatch with satellite data. In this study, we first tried to establish a grassland aboveground biomass estimation model at 1 m² quadrat scale by conducting synchronous experiments of unmanned aerial vehicle (UAV) and field measurement in three different grassland ecosystems. Two flight modes (the new QUADRAT mode and the commonly used MOSAIC mode) were used to generate point clouds for further processing. Canopy height metrics of each quadrat were then calculated using the canopy height model (CHM). Correlation analysis showed that the mean of the canopy height model (CHM_mean) had a significant linear relationship with field height (R² = 0.90, root mean square error (RMSE) = 19.79 cm, rRMSE = 16.5%, p < 0.001) and a logarithmic relationship with field aboveground biomass (R² = 0.89, RMSE = 91.48 g/m², rRMSE = 16.11%, p < 0.001). We concluded our study by conducting a preliminary application of estimation of the aboveground biomass at a plot scale by jointly using UAV and the constructed 1 m² quadrat scale estimation model. Our results confirmed that UAV could be used to collect large quantities of ground truths and bridge the scales between ground truth and remote sensing pixels, which were helpful in improving the accuracy of remote sensing inversion of grassland aboveground biomass.

Surface soil moisture (SSM) is a key limiting factor for vegetation growth in alpine meadow on the Qinghai-Tibetan Plateau (QTP). Patches with various sizes and types may cause the redistribution of SSM by changing soil hydrological processes, and then trigger or accelerate alpine grassland degradation. Therefore, it is vital to understand the effects of patchiness on SSM at multi-scales to provide a reference for alpine grassland restoration. However, there is a lack of direct observational evidence concerning the role of the size and type of patches on SSM, and little is known about the effects of patches pattern on SSM at plot scale. Here, we first measured SSM of typical patches with different sizes and types at patch scale and investigated their patterns and SSM spatial distribution through unmanned aerial vehicle (UAV)-mounted multi-type cameras at plot scale. We then analyzed the role of the size and type of patchiness on SSM at both patch and plot scales. Results showed that: (1) in situ measured SSM of typical patches was significantly different (P < 0.01), original vegetation patch (OV) had the highest SSM, followed by isolate vegetation patch (IV), small bare patch (SP), medium bare patch (MP) and large bare patch (LP); (2) the proposed method based on UAV images was able to estimate SSM (0–40 cm) with a satisfactory accuracy (R² = 0.89, P < 0.001); (3) all landscape indices of OV, with the exception of patch density, were positively correlated with SSM at plot scale, while most of the landscape indices of LP and IV showed negative correlations (P < 0.05). Our results indicated that patchiness intensified the spatial heterogeneity of SSM and potentially accelerated the alpine meadow degradation. Preventing the development of OV into IV and the expansion of LP is a critical task for alpine meadow management and restoration.

Plateau pika (Ochotona curzoniae, hereafter pika) is considered to exert a profound impact on vegetation species diversity of alpine grasslands. Great efforts have been made at mound or quadrat scales; nevertheless, there is still controversy about the effect of pika. It is vital to monitor vegetation species composition in natural heterogeneous ecosystems at a large scale to accurately evaluate the real role of pika. In this study, we performed field survey at 55 alpine grassland sites across the Shule River Basin using combined methods of aerial photographing using an unmanned aerial vehicle (UAV) and traditional ground measurement. Based on our UAV operation system, Fragmentation Monitoring and Analysis with aerial Photography (FragMAP), aerial images were acquired. Plot-scale vegetation species were visually identified, and total pika burrow exits were automatically retrieved using the self-developed image processing software. We found that there were significant linear relationships between the vegetation species diversity indexes obtained by these two methods. Additionally, the total number of identified species by the UAV method was 71, which was higher than the Quadrat method recognition, with the quantity of 63. Our results indicate that the UAV was suitable for long-term repeated monitoring vegetation species composition of multiple alpine grasslands at plot scale. With the merits of UAV, it confirmed that pika’s disturbance belonged to the medium level, with the density ranging from 30.17 to 65.53 ha⁻¹. Under this density level, pika had a positive effect on vegetation species diversity, particularly for the species richness of sedge and forb. These findings conclude that the UAV was an efficient and economic tool for species monitoring to reveal the role of pika in the alpine grasslands.

The Kobresia pygmaea (KP) community is a key succession stage of alpine meadow degradation on the Qinghai–Tibet Plateau (QTP). However, most of the grassland classification and mapping studies have been performed at the grassland type level. The spatial distribution and impact factors of KP on the QTP are still unclear. In this study, field measurements of the grassland vegetation community in the eastern part of the QTP (Counties of Zeku, Henan and Maqu) from 2015 to 2019 were acquired using unmanned aerial vehicle (UAV) technology. The machine learning algorithms for grassland vegetation community classification were constructed by combining Gaofen satellite images and topographic indices. Then, the spatial distribution of KP community was mapped. The results showed that: (1) For all field observed sites, the alpine meadow vegetation communities demonstrated a considerable spatial heterogeneity. The traditional classification methods can hardly distinguish those communities due to the high similarity of their spectral characteristics. (2) The random forest method based on the combination of satellite vegetation indices, texture feature and topographic indices exhibited the best performance in three counties, with overall accuracy and Kappa coefficient ranged from 74.06% to 83.92% and 0.65 to 0.80, respectively. (3) As a whole, the area of KP community reached 1434.07 km², and accounted for 7.20% of the study area. We concluded that the combination of satellite remote sensing, UAV surveying and machine learning can be used for KP classification and mapping at community level.

The fractional vegetation cover (FVC) data measured on the ground is the main source for the calibration and verification of FVC remote sensing inversion, and its accuracy directly affects the accuracy of remote sensing inversion results. However, the existing research on the evaluation of the accuracy of the field quadrat survey of FVC based on the satellite remote sensing pixel scale is inadequate, especially in the alpine grassland of the Qinghai-Tibet Plateau. In this paper, five different alpine grasslands were examined, the accuracy of the FVC obtained by the photography method was analyzed, and the influence of the number of samples on the field survey results was studied. First, the results show that the threshold method could accurately extract the vegetation information in the photos and obtain the FVC with high accuracy and little subjective interference. Second, the number of samples measured on the ground was logarithmically related to the accuracy of the FVC of the sample plot (p < 0.001). When the number of samples was larger, the accuracy of the FVC of the sample plot was higher and closer to the real value, and the stability of data also increased with the increase of the number of samples. Third, the average FVC of the measured quadrats on the ground was able to represent the FVC of the sample plot, but on the basis that there were enough measured quadrats. Finally, the results revealed that the degree of fragmentation reflecting the state of ground vegetation affects the acquisition accuracy of FVC. When the degree of fragmentation of the sample plot is higher, the number of samples needed to achieve the accuracy index is higher. Our results suggest that when obtaining the FVC on the satellite remote sensing pixel scale, the number of samples measured on the ground is an important factor affecting the accuracy, which cannot be ignored.

Fractional vegetation coverage (FVC) is an important indicator of ecosystem change. At present, FVC products are mainly concentrated at low and medium spatial resolution and lack high temporal and spatial resolution, which brings certain challenges to the fine monitoring of ecological environments. In this study, we evaluated the accuracy of four remote sensing inversion models for FVC based on high-spatial-resolution Sentinel-2 imagery and unmanned aerial vehicle (UAV) field-measured FVC data in 2019. Then the inversion models were optimized by constructing a multidimensional feature dataset. Finally, the Source Region of the Yellow River (SRYR) FVC product was created using the best inversion model, and the spatial-temporal variation characteristics of the FVC in the region were analyzed. The study’s findings revealed that: (1) The accuracies of the four FVC inversion models were as follows: the Gradient Boosting Decision Tree (GBDT) model (R² = 0.967, RMSE = 0.045) > Random Forest (RF) model (R² = 0.962, RMSE = 0.049) > Support Vector Machine (SVM) model (R² = 0.925, RMSE = 0.072) > Pixel Dichotomy (PD) model (R² = 0.869, RMSE = 0.097). (2) Constructing a multidimensional feature dataset to optimize the driving data can improve the accuracy of the inversion model. NDVI and elevation are important factors affecting the accuracy of machine learning inversion algorithms, and the visible blue band is the most important feature factor of the GBDT model. (3) The FVC in the SRYR gradually increased from west to east and from north to south. The change trajectories of grassland FVC from 2017 to 2022 were not significant. The areas that tend to improve were mainly distributed in the southeast (1.31%), while the areas that tend to degrade were mainly distributed in the central and northwest (1.89%). This study provides a high-spatial-resolution FVC inversion optimization scheme, which is of great significance for the fine monitoring of alpine grassland ecological environments.