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Bhartendu Nath Mishra
Department of Biotechnology, Institute of Engineering and Technology, Dr. A.P.J. Abdul Kalam Technical University, Lucknow 226021, India

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Journal article
Published: 29 July 2021 in Sustainability
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The members of the Liliaceae family are considered an excellent source of biologically active compounds. However, work on antimicrobial potential and characterization of the bioactive fractions of the Lilium philadelphicum flower is limited and needs to be explored. The present study reports the antimicrobial potential of the bioactive fraction extracted from the flower of L. philadelphicum (red lily) and partial characterization of the bioactive compound(s). The antimicrobial activity was tested against nine different Gram-positive and Gram-negative bacterial strains. The minimum inhibitory concentration (MIC) values of methanolic extract of the L.philadelphicum flower against Acinetobacterbouvetii, Achromobacterxylosoxidans, Bacillussubtilis MTCC 121, Candidaalbicans MTCC 183, Klebsiellapneumoniae MTCC 3384, and Salmonellatyphi MTCC 537 were 25, 50, 12.5, 50, 100, and 50 μg/mL, respectively. The phytochemical analysis of the extract revealed the presence of phenols, flavonoids, tannins, terpenoids, glycosides, coumarins, and quinones. The cytotoxicity of the partially purified compound against the HepG2 cell line using MTT assay demonstrated up to 90% cell viability with a bioactive compound concentration of 50 μg/mL. However, the increase in the bioactive compound’s concentration up to 1000 μg/mL resulted in nearly 80% cell viability. This minor decline in cell viability suggests the importance and suitability of the bioactive compound for therapeutic applications. Spectroscopic studies of the bioactive compound by UV-visible spectroscopy, FT-infrared spectroscopy, gas chromatography-mass spectrometry (GC-MS), as well as phytochemical analysis, suggested the presence of a terpenoid moiety, which may be responsible for the antimicrobial property of the L. philadelphicum flower.

ACS Style

Shefali Singh; Vineeta Singh; Alaa Alhazmi; Bhartendu Mishra; Shafiul Haque; R. Sayyed; Kumari Sunita. Lilium philadelphicum Flower as a Novel Source of Antimicrobial Agents: A Study of Bioactivity, Phytochemical Analysis, and Partial Identification of Antimicrobial Metabolites. Sustainability 2021, 13, 8471 .

AMA Style

Shefali Singh, Vineeta Singh, Alaa Alhazmi, Bhartendu Mishra, Shafiul Haque, R. Sayyed, Kumari Sunita. Lilium philadelphicum Flower as a Novel Source of Antimicrobial Agents: A Study of Bioactivity, Phytochemical Analysis, and Partial Identification of Antimicrobial Metabolites. Sustainability. 2021; 13 (15):8471.

Chicago/Turabian Style

Shefali Singh; Vineeta Singh; Alaa Alhazmi; Bhartendu Mishra; Shafiul Haque; R. Sayyed; Kumari Sunita. 2021. "Lilium philadelphicum Flower as a Novel Source of Antimicrobial Agents: A Study of Bioactivity, Phytochemical Analysis, and Partial Identification of Antimicrobial Metabolites." Sustainability 13, no. 15: 8471.

Journal article
Published: 26 June 2021 in Seminars in Cancer Biology
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The role of epigenetics in the etiology of cancer progression is being emphasized for the past two decades to check the impact of chromatin modifiers and remodelers. Histone modifications, DNA methylation, chromatin remodeling, nucleosome positioning, regulation by non-coding RNAs and precisely microRNAs are influential epigenetic marks in the field of progressive cancer sub-types. Furthermore, constant epigenetic changes due to hyper or hypomethylation could efficiently serve as effective biomarkers of cancer diagnosis and therapeutic development. Ongoing research in the field of epigenetics has resulted in the resolutory role of various epigenetic markers and their inhibition using specific inhibitors to arrest their key cellular functions in in-vitro and pre-clinical studies. Although, the mechanism of epigenetics in cancer largely remains unexplored. Nevertheless, various advancements in the field of epigenetics have been made through transcriptome analysis and in-vitro genome targeting technologies to unravel the applicability of epigenetic markers for future cancer therapeutics and management. Therefore, this review emphasizes on recent advances in epigenetic landscapes that could be targeted/explored using novel approaches as personalized treatment modalities for cancer containment.

ACS Style

Showket Hussain; Sonam Tulsyan; Sajad Ahmad Dar; Sandeep Sisodiya; Umme Abiha; Rakesh Kumar; Bhartendu Nath Mishra; Shafiul Haque. Role of Epigenetics in carcinogenesis: Recent Advancements in Anticancer Therapy. Seminars in Cancer Biology 2021, 1 .

AMA Style

Showket Hussain, Sonam Tulsyan, Sajad Ahmad Dar, Sandeep Sisodiya, Umme Abiha, Rakesh Kumar, Bhartendu Nath Mishra, Shafiul Haque. Role of Epigenetics in carcinogenesis: Recent Advancements in Anticancer Therapy. Seminars in Cancer Biology. 2021; ():1.

Chicago/Turabian Style

Showket Hussain; Sonam Tulsyan; Sajad Ahmad Dar; Sandeep Sisodiya; Umme Abiha; Rakesh Kumar; Bhartendu Nath Mishra; Shafiul Haque. 2021. "Role of Epigenetics in carcinogenesis: Recent Advancements in Anticancer Therapy." Seminars in Cancer Biology , no. : 1.

Original paper
Published: 30 January 2021 in Journal of Molecular Modeling
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Alzheimer’s disease (AD) is a type of brain disorder, wherein a person experiences gradual memory loss, state of confusion, hallucination, agitation, and personality change. AD is marked by the presence of extracellular amyloid plaques and intracellular neurofibrillary tangles (NFTs) and synaptic losses. Increased cases of AD in recent times created a dire need to discover or identify chemical compounds that can cease the development of AD. This study focuses on finding potential drug molecule(s) active against β-secretase, also known as β-site amyloid precursor protein cleaving enzyme 1 (BACE1). Clustering analysis followed by phylogenetic studies on microarray datasets retrieved from GEO browser showed that BACE1 gene has genetic relatedness with the RCAN1 gene. A ligand library comprising 60 natural compounds retrieved from literature and 25 synthetic compounds collected from DrugBank were screened. Further, 350 analogues of potential parent compounds were added to the library for the docking purposes. Molecular docking studies identified 11-oxotigogenin as the best ligand molecule. The compound showed the binding affinity of − 11.1 Kcal/mole and forms three hydrogen bonds with Trp124, Ile174, and Arg176. The protein-ligand complex was subjected to 25 ns molecular dynamics simulation and the potential energy of the complex was found to be − 1.24579e+06 Kcal/mole. In this study, 11-oxotigogenin has shown promising results against BACE1, which is a leading cause of AD, hence warrants for in vitro and in vivo validation of the same. In addition, in silico identification of 11-oxotigogenin as a potential anti-AD compound paves the way for designing of chemical scaffolds to discover more potent BACE1 inhibitors. Graphical abstract

ACS Style

Pragya Kushwaha; Vineeta Singh; Pallavi Somvanshi; Tulika Bhardwaj; George E. Barreto; Ghulam Md. Ashraf; Bhartendu Nath Mishra; Rajendra Singh Chundawat; Shafiul Haque. Identification of new BACE1 inhibitors for treating Alzheimer’s disease. Journal of Molecular Modeling 2021, 27, 1 -15.

AMA Style

Pragya Kushwaha, Vineeta Singh, Pallavi Somvanshi, Tulika Bhardwaj, George E. Barreto, Ghulam Md. Ashraf, Bhartendu Nath Mishra, Rajendra Singh Chundawat, Shafiul Haque. Identification of new BACE1 inhibitors for treating Alzheimer’s disease. Journal of Molecular Modeling. 2021; 27 (2):1-15.

Chicago/Turabian Style

Pragya Kushwaha; Vineeta Singh; Pallavi Somvanshi; Tulika Bhardwaj; George E. Barreto; Ghulam Md. Ashraf; Bhartendu Nath Mishra; Rajendra Singh Chundawat; Shafiul Haque. 2021. "Identification of new BACE1 inhibitors for treating Alzheimer’s disease." Journal of Molecular Modeling 27, no. 2: 1-15.

Review article
Published: 28 January 2021 in Journal of Environmental Chemical Engineering
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Nanotechnology, in terms of nanomaterials e.g. nanoparticles, polymeric nanomaterials, 2D nanosheets, or electrospun nanofibrous membranes, nanocomposites for desalination or water-treatment applications, is an emerging concept that has rapidly grown in interest as a method for improving the water quality. With the increasing knowledge of nanomaterial fabrication techniques, researchers throughout the globe are targeting more on the synthesis and manufacturing processes of nanomaterials for various applications. The process of electrospinning for making nanofibers completely allows synthetic and natural polymers incorporating different functional materials. Recently, scientists have proposed various types of electrospun membranes using fluorinated polymers and hydrocarbon polymers because of their intrinsic hydrophobicity. Electrospun membranes have been researched in several types of membrane separation and water purification processes. The application of electrospun nanofiber membranes in water purification constituting a primary research axis and experiments that have already been conducted in various laboratories are reviewed in this paper. In this review, recent updates are reviewed concerning the different applications of desalination and water treatment. Additionally, critical comments and the future of nanofiber applications in desalination and water treatment have been given in this review.

ACS Style

Vaidhegi Kugarajah; Atul Kumar Ojha; Shivendu Ranjan; Nandita Dasgupta; Mahesh Ganesapillai; Sangeetha Dharmalingam; Ahmad Elmoll; Seyed Ali Hosseini; Lakshmanan Muthulakshmi; Sekar Vijayakumar; Bhartendu Nath Mishra. Future applications of electrospun nanofibers in pressure driven water treatment: A brief review and research update. Journal of Environmental Chemical Engineering 2021, 9, 105107 .

AMA Style

Vaidhegi Kugarajah, Atul Kumar Ojha, Shivendu Ranjan, Nandita Dasgupta, Mahesh Ganesapillai, Sangeetha Dharmalingam, Ahmad Elmoll, Seyed Ali Hosseini, Lakshmanan Muthulakshmi, Sekar Vijayakumar, Bhartendu Nath Mishra. Future applications of electrospun nanofibers in pressure driven water treatment: A brief review and research update. Journal of Environmental Chemical Engineering. 2021; 9 (2):105107.

Chicago/Turabian Style

Vaidhegi Kugarajah; Atul Kumar Ojha; Shivendu Ranjan; Nandita Dasgupta; Mahesh Ganesapillai; Sangeetha Dharmalingam; Ahmad Elmoll; Seyed Ali Hosseini; Lakshmanan Muthulakshmi; Sekar Vijayakumar; Bhartendu Nath Mishra. 2021. "Future applications of electrospun nanofibers in pressure driven water treatment: A brief review and research update." Journal of Environmental Chemical Engineering 9, no. 2: 105107.

Review
Published: 05 January 2021 in Archives of Medical Science
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IntroductionThe role of interferon gamma (IFN-γ) +874 A>T (rs2430561) gene polymorphism has been evaluated in different ethnicities with pulmonary tuberculosis (PTB) infection, and inconsistent results have been reported. In this study, a meta-analysis was performed to determine the precise association between IFN-γ +874 A>T gene polymorphism and PTB susceptibility.Material and methodsA total of 21 studies comprising 4281 confirmed PTB cases and 5186 healthy controls were included in this meta-analysis by searching the PubMed (Medline), EMBASE, and Google Scholar web-databases.ResultsWe observed reduced risk of PTB in allelic contrast (T vs. A: p = 0.001; OR = 0.818, 95% CI: 0.723–0.926), homozygous (TT vs. AA: p = 0.017; OR = 0.715, 95% CI: 0.543–0.941), heterozygous (AT vs. AA: p = 0.002; OR = 0.782, 95% CI: 0.667–0.917), dominant (TT+AT vs. AA: p = 0.002; OR = 0.768, 95% CI: 0.652–0.906), and recessive (TT vs. AA+AT: p = 0.042; OR = 0.802, 95% CI: 0.649–0.992) genetic models. In ethnicity-wise subgroup analysis, reduced risk of PTB was found in the Caucasian population. However, we did not find an association with any of the genetic models in the Asian population.ConclusionsIn conclusion, the IFN-γ +874 A>T gene polymorphism is significantly associated with reduced risk of PTB, showing a protective effect in the overall and in the Caucasian population. However, this polymorphism is not associated with PTB risk in the Asian population.

ACS Style

Mohammed Y. Areeshi; Raju K. Mandal; Sajad A. Dar; Arshad Jawed; Mohd Wahid; Mohtashim Lohani; Aditya K. Panda; Bhartendu Nath Mishra; Naseem Akhter; Shafiul Haque. IFN-γ +874 A>T (rs2430561) gene polymorphism and risk of pulmonary tuberculosis: a meta-analysis. Archives of Medical Science 2021, 17, 177 -188.

AMA Style

Mohammed Y. Areeshi, Raju K. Mandal, Sajad A. Dar, Arshad Jawed, Mohd Wahid, Mohtashim Lohani, Aditya K. Panda, Bhartendu Nath Mishra, Naseem Akhter, Shafiul Haque. IFN-γ +874 A>T (rs2430561) gene polymorphism and risk of pulmonary tuberculosis: a meta-analysis. Archives of Medical Science. 2021; 17 (1):177-188.

Chicago/Turabian Style

Mohammed Y. Areeshi; Raju K. Mandal; Sajad A. Dar; Arshad Jawed; Mohd Wahid; Mohtashim Lohani; Aditya K. Panda; Bhartendu Nath Mishra; Naseem Akhter; Shafiul Haque. 2021. "IFN-γ +874 A>T (rs2430561) gene polymorphism and risk of pulmonary tuberculosis: a meta-analysis." Archives of Medical Science 17, no. 1: 177-188.

Journal article
Published: 23 December 2020 in Current Pharmaceutical Design
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Background: The global health emergency due to SARS-CoV-2 causing the COVID-19 pandemic emphasized the scientific community to intensify their research work for its therapeutic solution. In this study, Indian traditional spices owing to various medicinal properties were tested in silico for their inhibitory activity against SARS-CoV-2 proteins. SARS-CoV-2 spike proteins (SP) and main proteases (Mpro) play a significant role in infection development were considered as potential drug targets. Methods: A total of 75 phytochemicals present in traditional Indian spices retrieved from the published literature and Dr. Duke’s Phytochemical and Ethnobotanical Database, were docked with Mpro (PDB IDs: 6YNQ), and the SP (PDB IDs: 6LXT and 6YOR). Results: Through the screening process, 75 retrieved phytochemicals were docked with spike protein (PDB IDs: 6LXT and 6YOR) and main protease (PDB ID: 6YNQ) of SARS-CoV-2. Among them, myricetin, a flavonoid (rank score: 6LXT: -11.72383; 6YOR: -9.87943; 6YNQ: -11.68164) from Allium sativumL and Isovitexin, an example of flavone (rank score:6LXT: -12.14922; 6YOR: -10.19443; 6YNQ: - 12.60603) from Pimpinella anisumL were the most potent ligands against SP and Mproof SARS-CoV-2. Whereas, Astragalin from Crocus sativusL.; Rutin from Illicium verum, Oxyguttiferone from Garcinia cambogia; Scopolin from Apium graveolens L, Luteolin from Salvia officinalis, Emodin, Aloe-emodin from Cinnamomum zeylanicium and Apigenin from Allium sativumL showed better inhibition against Mpro than SP of SARS-CoV-2. The amino acid residues like SER, LYS, ASP and TYR were found playing important role in protein-ligand interactions via hydrogen bonding and Vander Waals forces. Conclusion: Optimal use of traditional spices in our daily meals may help fight against COVID-19. This study also paves the path for herbal drug formulation against SARS-CoV-2 after wet lab validation.

ACS Style

Brijesh Kumar; Sama Zaidi; Shafiul Haque; Nandita Dasgupta; Arif Hussain; Sreepoorna Unni; Vineeta Singh; Bhartendu Nath Mishra. In silico studies reveal antiviral effects of traditional Indian spices on COVID-19. Current Pharmaceutical Design 2020, 26, 1 -20.

AMA Style

Brijesh Kumar, Sama Zaidi, Shafiul Haque, Nandita Dasgupta, Arif Hussain, Sreepoorna Unni, Vineeta Singh, Bhartendu Nath Mishra. In silico studies reveal antiviral effects of traditional Indian spices on COVID-19. Current Pharmaceutical Design. 2020; 26 ():1-20.

Chicago/Turabian Style

Brijesh Kumar; Sama Zaidi; Shafiul Haque; Nandita Dasgupta; Arif Hussain; Sreepoorna Unni; Vineeta Singh; Bhartendu Nath Mishra. 2020. "In silico studies reveal antiviral effects of traditional Indian spices on COVID-19." Current Pharmaceutical Design 26, no. : 1-20.

Journal article
Published: 10 December 2020 in Current Pharmaceutical Design
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Background: The main proteases (Mpro) and Spike Proteins (SP) of Severe Acute Respiratory Syndrome Coronavirus 2 (SARSCoV-2) play a major role in viral infection development by producing several non-structural proteins (nsPs) and penetrating the host cells respectively. In this study, the potential of in silico molecular docking-based drug repositioning approach was exploited for identifying the inhibitors of Mpro and SP of SARS-CoV-2. Methods: A total of 196 compounds including various US-FDA-approved drugs, vitamins and their analogs were docked with Mpro (PDB IDs: 6YB7 and 6Y84), and the top six ligands were further tested for ADME properties followed by docking with SP (PDB IDs: 6LXT and 6W41). Results: Out of 196 compounds, binding energy (DE) of Silybin B (6YB7: DE: -11.20 kcal/mol; 6Y84: DE: -10.18 kcal/mol; 6LXT:DE: -10.47 kcal/mol; 6W41:DE: -10.96 kcal/mol) and Cianidanol (6YB7:DE: -8.85 kcal/mol; 6Y84:DE:-10.02 kcal/mol; 6LXT:DE:-9.36 kcal/mol; 6W41:DE: -9.52 kcal/mol) demonstrated better binding and ADME properties compared with the currently endeavored drugs like Hydroxychloroquine and Lopinavir. Additionally, Elliptinone, Diospyirin, SCHEMBL94263 and Fiboflavin have shown encouraging results. Fiboflavin, an immunity booster, was found to inhibit both the Mpro and spike protein of SARS-CoV-2. It was observed that amino acid residues MET6, ALA7, PHE8, PRO9, ASP295, GLY302, VAL303 and THR304 play significant roles in protein-ligand interactions through hydrogen bonds and Vander Waals forces. Conclusion: Silybin B and Cianidanol showed excellent binding and ADME properties compared with the currently endeavored drugs and can be exploited as therapeutic options against SARS-CoV-2 infection after experimental validation and clinical trials.

ACS Style

Rashi Srivastava; Shubham Tripathi; Sreepoorna Unni; Arif Hussain; Shafiul Haque; Nandita Dasgupta; Vineeta Singh; Bhartendu Nath Mishra. Silybin B and Cianidanol Inhibit M pro and Spike Protein of SARS-CoV-2: Evidence from in Silico Molecular Docking Studies. Current Pharmaceutical Design 2020, 26, 1 -16.

AMA Style

Rashi Srivastava, Shubham Tripathi, Sreepoorna Unni, Arif Hussain, Shafiul Haque, Nandita Dasgupta, Vineeta Singh, Bhartendu Nath Mishra. Silybin B and Cianidanol Inhibit M pro and Spike Protein of SARS-CoV-2: Evidence from in Silico Molecular Docking Studies. Current Pharmaceutical Design. 2020; 26 ():1-16.

Chicago/Turabian Style

Rashi Srivastava; Shubham Tripathi; Sreepoorna Unni; Arif Hussain; Shafiul Haque; Nandita Dasgupta; Vineeta Singh; Bhartendu Nath Mishra. 2020. "Silybin B and Cianidanol Inhibit M pro and Spike Protein of SARS-CoV-2: Evidence from in Silico Molecular Docking Studies." Current Pharmaceutical Design 26, no. : 1-16.

Journal article
Published: 18 September 2020 in LWT
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Fumonisins are an important class of mycotoxin due to its wide distribution, health hazard, and economic significance. In the current study, clove oil nanoemulsion was investigated to reduce the load of fumonisin from the maize kernels. The fabricated nanoemulsion of 103nm size range efficiently reduced the growth of Fusarium proliferatum sp. and HPLC data confirms the reduction in the load of fumonisin B1 and B2 to the maximum recommended level by European Union.Further, clove oil nanoemulsion was electrosprayed to form a ready to use formulation with a core shell matrix. Denatured whey protein has been used as wall material and 96.25% encapsulation efficiencywas achieved. . The improved antifungal activity on maize kernel and ready-to-use prototype has future possibilities to be scaled up at industrial scale.Thus, the encapsulated material can be used as a preventive measure to reduce the fumonisin load during storage conditions.

ACS Style

Priyanka Singh; Nandita Dasgupta; Vineeta Singh; Narayan Chandra Mishra; Hemant Singh; Shiv Dutt Purohit; Nidhi Srivastava; Shivendu Ranjan; Narayan Prasad Yadav; Bhartendu Nath Mishra. Inhibitory effect of clove oil nanoemulsion on fumonisin isolated from maize kernels. LWT 2020, 134, 110237 .

AMA Style

Priyanka Singh, Nandita Dasgupta, Vineeta Singh, Narayan Chandra Mishra, Hemant Singh, Shiv Dutt Purohit, Nidhi Srivastava, Shivendu Ranjan, Narayan Prasad Yadav, Bhartendu Nath Mishra. Inhibitory effect of clove oil nanoemulsion on fumonisin isolated from maize kernels. LWT. 2020; 134 ():110237.

Chicago/Turabian Style

Priyanka Singh; Nandita Dasgupta; Vineeta Singh; Narayan Chandra Mishra; Hemant Singh; Shiv Dutt Purohit; Nidhi Srivastava; Shivendu Ranjan; Narayan Prasad Yadav; Bhartendu Nath Mishra. 2020. "Inhibitory effect of clove oil nanoemulsion on fumonisin isolated from maize kernels." LWT 134, no. : 110237.

Journal article
Published: 12 December 2019 in Biomolecules
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The inexhaustible nature and biodegradability of bioplastics like polyhydroxyalkanoates (PHAs) make them suitable assets to replace synthetic plastics. The eventual fate of these eco-friendly and non-toxic bioplastics relies upon the endeavors towards satisfying cost and, in addition, execution necessity. In this study, we utilized and statistically optimized different food (kitchen-/agro-) waste as a sole carbon/nitrogen source for the production of PHA at a reduced cost, indicating a proficient waste administration procedure. Seven different types of kitchen-/agro-waste were used as unique carbon source and four different types of nitrogen source were used to study their impact on PHA production by Bacillus subtilis MTCC 144. Among four different studied production media, mineral salt medium (MSM) (biomass: 37.7 g/L; cell dry weight: 1.8 g/L; and PHA: 1.54 g/L) was found most suitable for PHA production. Further, carbon and nitrogen components of MSM were optimized using one-factor-at-a-time experiments, and found that watermelon rind (PHA = 12.97 g/L) and pulse peel (PHA = 13.5 g/L) were the most suitable carbon and nitrogen sources, respectively, in terms of PHA (78.60%) recovery. The concentrations of these factors (sources) were statistically optimized using response surface methodology coupled with the genetic algorithm approach. Additionally, in order to enhance microbial PHA production, the interaction of citrate synthase, a key enzyme in the TCA cycle, with different known inhibitors was studied using in silico molecular docking approach. The inhibition of citrate synthase induces the blockage of the tricarboxylic cycle (TCA), thereby increasing the concentration of acetyl-CoA that helps in enhanced PHA production. Molecular docking of citrate synthase with different inhibitors of PubChem database revealed that hesperidin (PubChem compound CID ID 10621), generally present in citrus fruits, is the most efficient inhibitor of the TCA cycle with the binding score of –11.4 and warrants experimental validation. Overall, this study provides an efficient food waste management approach by reducing the production cost and enhancing the production of PHA, thereby lessening our reliance on petroleum-based plastics.

ACS Style

Apoorva Rao; Shafiul Haque; Hesham A. El-Enshasy; Vineeta Singh; Bhartendu Nath Mishra. RSM–GA Based Optimization of Bacterial PHA Production and In Silico Modulation of Citrate Synthase for Enhancing PHA Production. Biomolecules 2019, 9, 872 .

AMA Style

Apoorva Rao, Shafiul Haque, Hesham A. El-Enshasy, Vineeta Singh, Bhartendu Nath Mishra. RSM–GA Based Optimization of Bacterial PHA Production and In Silico Modulation of Citrate Synthase for Enhancing PHA Production. Biomolecules. 2019; 9 (12):872.

Chicago/Turabian Style

Apoorva Rao; Shafiul Haque; Hesham A. El-Enshasy; Vineeta Singh; Bhartendu Nath Mishra. 2019. "RSM–GA Based Optimization of Bacterial PHA Production and In Silico Modulation of Citrate Synthase for Enhancing PHA Production." Biomolecules 9, no. 12: 872.

Forum
Published: 05 December 2019 in Trends in Plant Science
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Here we highlight advances and opportunities for using 3D bioprinting in plant biology research that could lead to low-cost solutions for biomedical and other applications. For example, the development of plant cell-based and plant-inspired 3D-printed constructs could provide information about single-cell, tissue, and whole-plant interactions with the surrounding environment.

ACS Style

Shakti Mehrotra; Smita Kumar; Vikas Srivastava; Taijshee Mishra; Bhartendu Nath Mishra. 3D Bioprinting in Plant Science: An Interdisciplinary Approach. Trends in Plant Science 2019, 25, 9 -13.

AMA Style

Shakti Mehrotra, Smita Kumar, Vikas Srivastava, Taijshee Mishra, Bhartendu Nath Mishra. 3D Bioprinting in Plant Science: An Interdisciplinary Approach. Trends in Plant Science. 2019; 25 (1):9-13.

Chicago/Turabian Style

Shakti Mehrotra; Smita Kumar; Vikas Srivastava; Taijshee Mishra; Bhartendu Nath Mishra. 2019. "3D Bioprinting in Plant Science: An Interdisciplinary Approach." Trends in Plant Science 25, no. 1: 9-13.

Journal article
Published: 21 November 2019 in Biomolecules
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Nanoparticles (NPs) possessing antibacterial activity represent an effective way of overcoming bacterial resistance. In the present work, we report a novel formulation of a nanoantibiotic formed using Ampicillin/sulbactam (Ams) and a zinc oxide nanoparticle (ZnO NP). ‘ZnO NP–Ams’ nanoantibiotic formulation is optimized using response surface methodology coupled genetic algorithm approach. The optimized formulation of nanoantibiotic (ZnO NP: 49.9 μg/mL; Ams: 33.6 μg/mL; incubation time: 27 h) demonstrated 15% enhanced activity compared to the unoptimized formulation against K. pneumoniae. The reactive oxygen species (ROS) generation was directly proportional to the interaction time of nanoantibiotic and K. pneumoniae after the initial lag phase of ~18 h as evident from 2s’-7’-Dichlorodihydrofluorescein diacetate assay. A low minimum inhibitory concentration (6.25 μg/mL) of nanoantibiotic formulation reveals that even a low concentration of nanoantibiotic can prove to be effective against K. pneumoniae. The importance of nanoantibiotic formulation is also evident by the fact that the 100 μg/mL of Ams and 25 µg of ZnO NP was required individually to inhibit the growth of K. pneumonia, whereas only 6.25 μg/mL of optimized nanoantibiotic formulation (ZnO NP and Ams in the ratio of 49.9: 33.6 in μg/mL and conjugation time of 27 h) was needed for the same.

ACS Style

Nidhi Sharma; Vineeta Singh; Asheesh Kumar Pandey; Bhartendu Nath Mishra; Maria Kulsoom; Nandita Dasgupta; Saif Khan; Hesham A. El-Enshasy; Shafiul Haque; Khan; El- Enshasy. Preparation and Evaluation of the ZnO NP–Ampicillin/Sulbactam Nanoantibiotic: Optimization of Formulation Variables Using RSM Coupled GA Method and Antibacterial Activities. Biomolecules 2019, 9, 764 .

AMA Style

Nidhi Sharma, Vineeta Singh, Asheesh Kumar Pandey, Bhartendu Nath Mishra, Maria Kulsoom, Nandita Dasgupta, Saif Khan, Hesham A. El-Enshasy, Shafiul Haque, Khan, El- Enshasy. Preparation and Evaluation of the ZnO NP–Ampicillin/Sulbactam Nanoantibiotic: Optimization of Formulation Variables Using RSM Coupled GA Method and Antibacterial Activities. Biomolecules. 2019; 9 (12):764.

Chicago/Turabian Style

Nidhi Sharma; Vineeta Singh; Asheesh Kumar Pandey; Bhartendu Nath Mishra; Maria Kulsoom; Nandita Dasgupta; Saif Khan; Hesham A. El-Enshasy; Shafiul Haque; Khan; El- Enshasy. 2019. "Preparation and Evaluation of the ZnO NP–Ampicillin/Sulbactam Nanoantibiotic: Optimization of Formulation Variables Using RSM Coupled GA Method and Antibacterial Activities." Biomolecules 9, no. 12: 764.

Articles
Published: 23 September 2019 in Journal of Biomolecular Structure and Dynamics
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The abnormal ubiquitin-proteasome is found as an important target in various human diseases, especially in cancer, and recently it has received prevalent attention as a challenging therapeutic target. The current work is designed to derive a predictive two- dimensional quantitative structure-activity relationship model for anticancer human proteasome target of NF-κB signaling pathway. The established 2D-QSAR is dependent on multiple linear regression approach and validated through leave-One-Out and external test set prediction method. The robust QSAR model showed the r2 of 0.83 and q2 of 0.80 and pred_r2 of 0.77. Three chemical properties, electronegativity count, average potential, and T_2_N_6 was identified as significant descriptors to predict the anticancer activities of the proteasome antagonists. Besides, the predicted top hit compounds were considered to check out the compliance with Rule of five and pharmacokinetic parameters for oral bioavailability in the human body. The molecular docking was accomplished to unravel the molecular mode of action of best-predicted compounds which was compatible with the standard drug. Following this approach, lastly two compounds NP and AP were recognized as the best candidates since these top compounds follow all the standard limit point of entire filters and indicated effective and decent docking score. The outcomes of the study sturdily suggested that the developed model and top hit compound’s binding conformation are rational in the exploration of unknown antagonist’s anticancer activity. The research would be of great support and is supposed to be of immense significance in the development and designing of drug-like candidates in preliminary drug discovery.

ACS Style

Deepika Yadav; Bhartendu Nath Mishra; Feroz Khan. Quantitative structure-activity relationship and molecular docking studies on human proteasome inhibitors for anticancer activity targeting NF-κB signaling pathway. Journal of Biomolecular Structure and Dynamics 2019, 1 -12.

AMA Style

Deepika Yadav, Bhartendu Nath Mishra, Feroz Khan. Quantitative structure-activity relationship and molecular docking studies on human proteasome inhibitors for anticancer activity targeting NF-κB signaling pathway. Journal of Biomolecular Structure and Dynamics. 2019; ():1-12.

Chicago/Turabian Style

Deepika Yadav; Bhartendu Nath Mishra; Feroz Khan. 2019. "Quantitative structure-activity relationship and molecular docking studies on human proteasome inhibitors for anticancer activity targeting NF-κB signaling pathway." Journal of Biomolecular Structure and Dynamics , no. : 1-12.

Journal article
Published: 31 August 2019 in Process Biochemistry
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In this study, one bioactive coumarin analog was obtained as a result of biotransformation of three inactive coumarin derivatives by free cells of Candida albicans. The bioactive analog was purified by Column chromatography and HPLC. The presence of coumarin moiety in the biotrasformed product was confirmed by λmax at 350-400 nm and FT-IR spectrum. The structure of the purified compound established by LC-MS and 1H-NMR suggests the chances of biotransformation of 7-(3-(cyclopropylamino)-2-hydroxypropoxy)-4-methyl-2H-chromen-2-one (MW 289 Da) into 7-(3-Cyclopropylamino-2-hydroxy-propoxy)-4-methoxymethyl-chromen-2-one or 7-(3-Cyclopropylamino-2-methoxy-propoxy)-4-hydroxymethyl-chromen-2-one as a main product (MW 318 Da). The extra peak of 332 Da in LC-MS further confirms the presence of small proportion of 7-(3-Cyclopropylamino-2-methoxy-propoxy)-4-methoxymethyl-chromen-2-one apart from the main product. Oxidation followed by methylation reaction might be responsible for this conversion. The biotransformed product showed antimicrobial activity against Bacillus pumilus, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae and Salmonella typhi followed by decent antioxidant activity (6.756 µg IC50). The efficacy of coumarin-analog on cellular proliferation was found at 40 µM concentration against human breast cancer MDA-MB-231 cells in MTT assay, which is insignificant against normal breast tissue MCF-10A cells at the same concentration. These findings suggest the potential use of C. albicans for achieving pharmacologically active coumarin analogs showing antibacterial, antioxidant and cytotoxic activity.

ACS Style

Ambreen; Shafiul Haque; Vineeta Singh; Diksha Katiyar; Mohd Tariq Ali Khan; Vikash Chandra Tripathi; Hesham El Enshasy; Mukesh Pasupuleti; Bhartendu Nath Mishra. Biotransformation of newly synthesized coumarin derivatives by Candida albicans as potential antibacterial, antioxidant and cytotoxic agents. Process Biochemistry 2019, 87, 138 -144.

AMA Style

Ambreen, Shafiul Haque, Vineeta Singh, Diksha Katiyar, Mohd Tariq Ali Khan, Vikash Chandra Tripathi, Hesham El Enshasy, Mukesh Pasupuleti, Bhartendu Nath Mishra. Biotransformation of newly synthesized coumarin derivatives by Candida albicans as potential antibacterial, antioxidant and cytotoxic agents. Process Biochemistry. 2019; 87 ():138-144.

Chicago/Turabian Style

Ambreen; Shafiul Haque; Vineeta Singh; Diksha Katiyar; Mohd Tariq Ali Khan; Vikash Chandra Tripathi; Hesham El Enshasy; Mukesh Pasupuleti; Bhartendu Nath Mishra. 2019. "Biotransformation of newly synthesized coumarin derivatives by Candida albicans as potential antibacterial, antioxidant and cytotoxic agents." Process Biochemistry 87, no. : 138-144.

Journal article
Published: 28 June 2019 in Current Bioinformatics
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Background: Neurodevelopmental Disorders (NDDs) are impairment of the growth and development of the brain or central nervous system, which occurs at the developmental stage. This can include developmental brain dysfunction, which can manifest as neuropsychiatric problems or impaired motor function, learning, language or non-verbal communication. These include the array of disorder, including: Autism Spectrum Disorders (ASD), Attention Deficit Hyperactivity Disorders (ADHD) etc. There is no particular diagnosis and cure for NDDs. These disorders seem to be result from a combination of genetic, biological, psychosocial and environmental risk factors. Diverse scientific literature reveals the adverse effect of environmental factors specifically, exposure of pesticides, which leads to growing number of human pathological conditions; among these, neurodevelopmental disorder is an emerging issue nowadays. Objective: The current study focused on in silico identification of potential drug targets for pesticides induced neurodevelopmental disorder including Attention Deficit Hyperactivity Disorder (ADHD) and Autism Spectrum Disorder (ASD) and to design potential drug molecule for the target through drug discovery approaches. Methods: We identified 139 candidate genes for ADHD and 206 candidate genes for ASD from the NCBI database for detailed study. Protein-protein interaction network analysis was performed to identify key genes/proteins in the network by using STRING 10.0 database and Cytoscape 3.3.0 software. The 3D structure of target protein was built and validated. Molecular docking was performed against twenty seven possible phytochemicals i.e. beta amyrin, ajmaline, serpentine, urosolic, huperzine A etc. having neuroprotective activity. The best-docked compound was identified by the lowest Binding Energy (BE). Further, the prediction of drug-likeness and bioactivity analysis of leads were performed by using molinspiration cheminformatics software. Result & Conclusion: Based on betweenness centrality and node degree as a network topological parameter, solute carrier family 6 member 4 (SLC6A4) was identified as a common key protein in both the networks. 3-D structure of SLC6A4 protein was designed and validated respectively. Based on the lowest binding energy, beta amyrin (B.E = -8.54 kcal/mol) was selected as a potential drug candidate against SLC6A4 protein. Prediction of drug-likeness and bioactivity analysis of leads showed drug candidate as a potential inhibitor. Beta amyrin (CID: 73145) was obtained as the most potential therapeutic inhibitor for ASD & ADHD in human.

ACS Style

Shifa Ansari And Prachi Srivastava Neha Srivastava; Bhartendu Nath Mishra; Prachi Srivastava. In-Silico Identification of Drug Lead Molecule Against Pesticide Exposed-neurodevelopmental Disorders Through Network-Based Computational Model Approach. Current Bioinformatics 2019, 14, 460 -467.

AMA Style

Shifa Ansari And Prachi Srivastava Neha Srivastava, Bhartendu Nath Mishra, Prachi Srivastava. In-Silico Identification of Drug Lead Molecule Against Pesticide Exposed-neurodevelopmental Disorders Through Network-Based Computational Model Approach. Current Bioinformatics. 2019; 14 (5):460-467.

Chicago/Turabian Style

Shifa Ansari And Prachi Srivastava Neha Srivastava; Bhartendu Nath Mishra; Prachi Srivastava. 2019. "In-Silico Identification of Drug Lead Molecule Against Pesticide Exposed-neurodevelopmental Disorders Through Network-Based Computational Model Approach." Current Bioinformatics 14, no. 5: 460-467.

Journal article
Published: 24 April 2019 in Scientific Reports
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Arterial/venous thrombosis is the major cardiovascular disorder accountable for substantial mortality; and the current demand for antithrombotic agents is extensive. Heparinases depolymerize unfractionated heparin (UFH) for the production of low molecular-weight heparins (LMWHs; used as anticoagulants against thrombosis). A microbial strain of Streptomyces sp. showing antithrombotic activity was isolated from the soil sample collected from north India. The strain was characterized by using 16S rRNA homology technique and identified as Streptomyces variabilis MTCC 12266 capable of producing heparinase enzyme. This is the very first communication reporting Streptomyces genus as the producer of heparinase. It was observed that the production of intracellular heparinase was [63.8 U/mg protein (specific activity)] 1.58 folds higher compared to extracellular heparinase [40.28 U/mg protein]. DEAE-Sephadex A-50 column followed by Sepharose-6B column purification of the crude protein resulted 19.18 folds purified heparinase. SDS-PAGE analysis of heparinase resulted an estimated molecular-weight of 42 kDa. It was also found that intracellular heparinase has the ability to depolymerize heparin to generate LMWHs. Further studies related to the mechanistic action, structural details, and genomics involved in heparinase production from Streptomyces variabilis are warranted for large scale production/purification optimization of heparinase for antithrombotic applications.

ACS Style

Vineeta Singh; Shafiul Haque; Vibha Kumari; Hesham A. El-Enshasy; Bhartendu Nath Mishra; Pallavi Somvanshi; C. K. M. Tripathi. Isolation, Purification, and Characterization of Heparinase from Streptomyces variabilis MTCC 12266. Scientific Reports 2019, 9, 6482 .

AMA Style

Vineeta Singh, Shafiul Haque, Vibha Kumari, Hesham A. El-Enshasy, Bhartendu Nath Mishra, Pallavi Somvanshi, C. K. M. Tripathi. Isolation, Purification, and Characterization of Heparinase from Streptomyces variabilis MTCC 12266. Scientific Reports. 2019; 9 (1):6482.

Chicago/Turabian Style

Vineeta Singh; Shafiul Haque; Vibha Kumari; Hesham A. El-Enshasy; Bhartendu Nath Mishra; Pallavi Somvanshi; C. K. M. Tripathi. 2019. "Isolation, Purification, and Characterization of Heparinase from Streptomyces variabilis MTCC 12266." Scientific Reports 9, no. 1: 6482.

Review
Published: 27 February 2019 in Microbial Pathogenesis
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Interferon-γ (IFN-γ) plays a crucial role in immunological responses against Mycobacterium tuberculosis (M.tb) infection. The polymorphism at +874 A > T (rs2430561) influences the levels of IFN-γ, which may further influence the susceptibility to extrapulmonary tuberculosis (EPTB). This polymorphism has been investigated with respect to EPTB occurrence in different populations and provided contradictory and conflicting results. This study was performed to meta-statistically analyze the data and draw a more accurate conclusion regarding the association of IFN-γ +874 A > T gene polymorphism and EPTB susceptibility. A quantitative synthesis was executed for the pertinent studies retrieved from online web-databases viz. Google Scholar, PubMed/Medline and EMBASE. The pooled odds ratios (ORs) and confidence intervals (95% CIs) were estimated for all the genetic models by meta-analysis. A total of eight studies were retrieved which included 762 confirmed EPTB cases and 1341 controls. The meta-analysis results revealed reduced association of EPTB in allelic contrast (T vs. A: p = 0.001; OR = 0.668, 95% CI = 0.524 to 0.850), homozygous (TT vs. AA: p = 0.017; OR = 0.450, 95% CI = 0.234 to 0.868), heterozygous (AT vs. AA: p p = 0.004; OR = 0.574, 95% CI = 0.395 to 0.835), dominant (TT + AT vs. AA: p = 0.003; OR = 0.536, 95% CI = 0.354 to 0.810) and recessive (TT vs. AA + AT: p = 0.039; OR = 0.662, 95% CI = 0.448 to 0.980) genetic models. Furthermore, re-sampling statistics also revealed reduced risk of EPTB in overall population and Asian subgroup. This meta-analysis concluded that IFN-γ +874 A > T gene polymorphism is meaningfully related with the reduced EPTB risk in overall and Asian population, and further necessitates larger studies to be conducted on this topic in other races.

ACS Style

Raju K. Mandal; Mohd Wahid; Arshad Jawed; Sajad A. Dar; Aditya K. Panda; Naseem Akhter; Mohtashim Lohani; Bhartendu Nath Mishra; Saif Khan; Mohammed Y. Areeshi; Shafiul Haque. A trial sequential meta-analysis of IFN-γ +874 A>T (rs2430561) gene polymorphism and extrapulmonary tuberculosis risk. Microbial Pathogenesis 2019, 130, 1 -9.

AMA Style

Raju K. Mandal, Mohd Wahid, Arshad Jawed, Sajad A. Dar, Aditya K. Panda, Naseem Akhter, Mohtashim Lohani, Bhartendu Nath Mishra, Saif Khan, Mohammed Y. Areeshi, Shafiul Haque. A trial sequential meta-analysis of IFN-γ +874 A>T (rs2430561) gene polymorphism and extrapulmonary tuberculosis risk. Microbial Pathogenesis. 2019; 130 ():1-9.

Chicago/Turabian Style

Raju K. Mandal; Mohd Wahid; Arshad Jawed; Sajad A. Dar; Aditya K. Panda; Naseem Akhter; Mohtashim Lohani; Bhartendu Nath Mishra; Saif Khan; Mohammed Y. Areeshi; Shafiul Haque. 2019. "A trial sequential meta-analysis of IFN-γ +874 A>T (rs2430561) gene polymorphism and extrapulmonary tuberculosis risk." Microbial Pathogenesis 130, no. : 1-9.

Meta analysis
Published: 15 January 2019 in Bioscience Reports
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Hepatotoxicity is a severe problem generally faced by tuberculosis (TB) patients. It is a well-known adverse reaction due to anti-TB drugs in TB patients undergoing long-term treatment. The studies published previously have explored the connection of N-acetyltransferase 2 (NAT2) gene polymorphisms with isoniazid-induced hepatotoxicity, but the results obtained were inconsistent and inconclusive. A comprehensive trial sequence meta-analysis was conducted employing 12 studies comprising 3613 controls and 933 confirmed TB cases using the databases namely, EMBASE, PubMed (Medline) and Google Scholar till December 2017. A significant association was observed with individuals carrying variant allele at position 481C>T (T vs. C: P = 0.001; OR = 1.278, 95% CI = 1.1100–1.484), at position 590G>A (A vs. G: P = 0.002; OR = 1.421, 95% CI = 1.137–1.776) and at position 857G>A (A vs. G: P = 0.0022; OR = 1.411, 95% CI = 1.052–1.894) to higher risk of hepatotoxicity vis-à-vis wild-type allele. Likewise, the other genetic models of NAT2 gene polymorphisms have also shown increased risk of hepatotoxicity. No evidence of publication bias was observed. These results suggest that genetic variants of NAT2 gene have significant role in isoniazid induced hepatotoxicity. Thus, NAT2 genotyping has the potential to improve the understanding of the drug–enzyme metabolic capacity and help in early predisposition of isoniazid-induced hepatotoxicity.

ACS Style

Saif Khan; Raju K. Mandal; Abdulbaset Mohamed Elasbali; Sajad Dar; Arshad Jawed; Mohd Wahid; Harishankar Mahto; Mohtashim Lohani; Bhartendu Nath Mishra; Naseem Akhter; Ali Rabaan; Shafiul Haque. Pharmacogenetic association between NAT2 gene polymorphisms and isoniazid induced hepatotoxicity: trial sequence meta-analysis as evidence. Bioscience Reports 2019, 39, 1 .

AMA Style

Saif Khan, Raju K. Mandal, Abdulbaset Mohamed Elasbali, Sajad Dar, Arshad Jawed, Mohd Wahid, Harishankar Mahto, Mohtashim Lohani, Bhartendu Nath Mishra, Naseem Akhter, Ali Rabaan, Shafiul Haque. Pharmacogenetic association between NAT2 gene polymorphisms and isoniazid induced hepatotoxicity: trial sequence meta-analysis as evidence. Bioscience Reports. 2019; 39 (1):1.

Chicago/Turabian Style

Saif Khan; Raju K. Mandal; Abdulbaset Mohamed Elasbali; Sajad Dar; Arshad Jawed; Mohd Wahid; Harishankar Mahto; Mohtashim Lohani; Bhartendu Nath Mishra; Naseem Akhter; Ali Rabaan; Shafiul Haque. 2019. "Pharmacogenetic association between NAT2 gene polymorphisms and isoniazid induced hepatotoxicity: trial sequence meta-analysis as evidence." Bioscience Reports 39, no. 1: 1.

Review
Published: 15 January 2019 in Bioscience Reports
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Purpose: Tumor necrosis factor-α (TNF-α), secreted by the activated macrophages, may participate in the onset and progression of colorectal cancer (CRC). The association of TNF-α –308 G>A (rs1800629) single-nucleotide polymorphism (SNP) with CRC risk has been investigated by many studies but the results are inconclusive. A trial sequential meta-analysis was performed for precise estimation of the relationship between TNF-α –308 G>A gene polymorphism with CRC risk. Methods: Medline (PubMed), EMBASE (Excerpta-Medica) and Google Scholar were mined for relevant articles. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to estimate the significance of association. Results: The pooled analysis indicated no risk associated with TNF-α –308 G>A SNP and overall CRC risk in five genetic comparison models, i.e. allelic (A vs. G: P = 0.524; OR = 1.074, 95% CI = 0.863–1.335), homozygous (AA vs. GG: P = 0.489; OR = 1.227, 95% CI = 0.688–2.188), heterozygous (AG vs. GG: P = 0.811; OR = 1.024, 95% CI = 0.843–1.244), dominant (AA+AG vs. GG: P = 0.630; OR = 1.055, 95% CI = 0.849–1.311) and recessive (AA vs. AG+GG: P = 0.549; OR = 1.181, 95% CI = 0.686–2.033). Subgroup analysis revealed that TNF-α –308 G>A SNP is associated with reduced risk of CRC in Asian ethnicity. The study showed no publication bias. Conclusions: No association of TNF-α –308 G>A SNP with overall CRC risk was found. This SNP is likely to be protective against CRC in Asian population when compared with Caucasian population. Larger prospective-epidemiological studies are warranted to elucidate the roles of TNF-α –308 G>A SNP in the etiology of CRC and to endorse the present findings.

ACS Style

Raju K. Mandal; Munawwar Ali Khan; Arif Hussain; Naseem Akhter; Arshad Jawed; Sajad Dar; Mohd Wahid; Aditya K Panda; Mohtashim Lohani; Bhartendu Nath Mishra; Shafiul Haque. A trial sequential meta-analysis of TNF-α –308G>A (rs800629) gene polymorphism and susceptibility to colorectal cancer. Bioscience Reports 2019, 39, 1 .

AMA Style

Raju K. Mandal, Munawwar Ali Khan, Arif Hussain, Naseem Akhter, Arshad Jawed, Sajad Dar, Mohd Wahid, Aditya K Panda, Mohtashim Lohani, Bhartendu Nath Mishra, Shafiul Haque. A trial sequential meta-analysis of TNF-α –308G>A (rs800629) gene polymorphism and susceptibility to colorectal cancer. Bioscience Reports. 2019; 39 (1):1.

Chicago/Turabian Style

Raju K. Mandal; Munawwar Ali Khan; Arif Hussain; Naseem Akhter; Arshad Jawed; Sajad Dar; Mohd Wahid; Aditya K Panda; Mohtashim Lohani; Bhartendu Nath Mishra; Shafiul Haque. 2019. "A trial sequential meta-analysis of TNF-α –308G>A (rs800629) gene polymorphism and susceptibility to colorectal cancer." Bioscience Reports 39, no. 1: 1.

Original research
Published: 01 January 2019 in Infection and Drug Resistance
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Association of MBL2 gene polymorphisms with pulmonary tuberculosis susceptibility: trial sequence meta-analysis as evidence Raju K Mandal,1,* Munawwar Ali Khan,2,* Arif Hussain,3 Sajad A Dar,1 Sultan Aloufi,4 Arshad Jawed,1 Mohd Wahid,1 Aditya K Panda,5 Mohtashim Lohani,6 Naseem Akhter,7 Saif Khan,8 Bhartendu Nath Mishra,9 Shafiul Haque1 1Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan, Saudi Arabia; 2Department of Life and Environmental Sciences, College of Natural and Health Sciences, Zayed University, Dubai, United Arab Emirates; 3School of Life Sciences, Manipal Academy of Higher Education, Dubai, United Arab Emirates; 4Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Ha’il, Ha’il, Saudi Arabia; 5Centre for Life Sciences, Central University of Jharkhand, Ranchi, Jharkhand, India; 6Department of Emergency Medical Services, College of Applied Medical Sciences, Jazan University, Jazan, Saudi Arabia; 7Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Al Baha University, Al Baha, Saudi Arabia; 8Department of Basic Sciences, College of Dentistry, University of Ha’il, Ha’il, Saudi Arabia; 9Department of Biotechnology, Institute of Engineering and Technology, Lucknow, Uttar Pradesh, India *These authors contributed equally to this work Background: Mannose-binding lectin (MBL) or mannose-binding protein (MBP), encoded by MBL2 gene and secreted by the liver, activates complement system through lectin pathway in innate immunity against the host’s infection. Conflictingly, a number of MBL2 variants, rs1800450 (A>B), rs1800451 (A>C), rs5030737 (A>D), rs7096206 (Y>X), rs11003125 (H>L), and rs7095891 (P>Q) allele, have been found to be associated with compromised serum levels and pulmonary tuberculosis (PTB) susceptibility. The present meta-analysis study was performed to evaluate the potential association of these MBL2 gene variants with PTB susceptibility.Materials and methods: A quantitative synthesis was performed on PubMed (Medline), EMBASE, and Google Scholar web database searches. A meta-analysis was performed to calculate the pooled odds ratios and 95% CIs for all the genetic models.Results: A total of 14 eligible studies were included to analyze their pooled data for associations between alleles, genotypes, and minor allele carriers. The statistical analysis revealed the significant reduced PTB risk with homozygous variant genotype of rs1800451 polymorphism (CC vs AA: P=0.043; OR =0.828, 95% CI =0.689–0.994). Contrary to this, the variant allele of rs5030737 polymorphism showed association with increased PTB risk (D vs A: P=0.026; OR =1.563, 95% CI =1.054–2.317). However, the other genetic models of rs1800450 (A>B), rs7096206 (Y>X), and rs11003125 (H>L) MBL2 gene polymorphisms did not divulge any association with PTB susceptibility.Conclusion: The current meta-analysis concludes that rs1800451 (A>C) and rs5030737 (A>D) polymorphisms of MBL2 gene play a significant role in PTB susceptibility. Further, well-designed epidemiological studies with larger sample size including consideration of environmental factors are warranted for the future. Keywords: meta-analysis, mannose-binding lectin, MBL2, pulmonary tuberculosis, PTB, polymorphism

ACS Style

Raju K Mandal; Munawwar Ali Khan; Arif Hussain; Sajad A. Dar; Sultan Aloufi; Arshad Jawed; Mohd Wahid; Aditya K Panda; Mohtashim Lohani; Naseem Akhter; Saif Khan; Bhartendu Nath Mishra; Shafiul Haque. Association of MBL2 gene polymorphisms with pulmonary tuberculosis susceptibility: trial sequence meta-analysis as evidence. Infection and Drug Resistance 2019, ume 12, 185 -210.

AMA Style

Raju K Mandal, Munawwar Ali Khan, Arif Hussain, Sajad A. Dar, Sultan Aloufi, Arshad Jawed, Mohd Wahid, Aditya K Panda, Mohtashim Lohani, Naseem Akhter, Saif Khan, Bhartendu Nath Mishra, Shafiul Haque. Association of MBL2 gene polymorphisms with pulmonary tuberculosis susceptibility: trial sequence meta-analysis as evidence. Infection and Drug Resistance. 2019; ume 12 ():185-210.

Chicago/Turabian Style

Raju K Mandal; Munawwar Ali Khan; Arif Hussain; Sajad A. Dar; Sultan Aloufi; Arshad Jawed; Mohd Wahid; Aditya K Panda; Mohtashim Lohani; Naseem Akhter; Saif Khan; Bhartendu Nath Mishra; Shafiul Haque. 2019. "Association of MBL2 gene polymorphisms with pulmonary tuberculosis susceptibility: trial sequence meta-analysis as evidence." Infection and Drug Resistance ume 12, no. : 185-210.

Retraction of publication
Published: 31 December 2018 in Bioinformation
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[This retracts the article on p. 516 in vol. 11, PMID: 26912955.].

ACS Style

Feroz Khan; Bhartendu Nath Mishra. Retraction Note for Singh et al. Performance evaluation of DNA Motif discovery programs Published on December 31, 2018. Bioinformation 2018, 14, 594 -594.

AMA Style

Feroz Khan, Bhartendu Nath Mishra. Retraction Note for Singh et al. Performance evaluation of DNA Motif discovery programs Published on December 31, 2018. Bioinformation. 2018; 14 (9):594-594.

Chicago/Turabian Style

Feroz Khan; Bhartendu Nath Mishra. 2018. "Retraction Note for Singh et al. Performance evaluation of DNA Motif discovery programs Published on December 31, 2018." Bioinformation 14, no. 9: 594-594.