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Marion Langeheine
Institute of Anatomy, University of Veterinary Medicine Foundation, 30173 Hanover, Germany

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Journal article
Published: 25 July 2021 in International Journal of Molecular Sciences
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Testicular Connexin43 (Cx43) connects adjacent Sertoli cells (SC) and SC to germ cells (GC) in the seminiferous epithelium and plays a crucial role in spermatogenesis. However, the distinction whether this results from impaired inter-SC communication or between GC and SC is not possible, so far. Thus, the question arises, whether a GC-specific Cx43 KO has similar effects on spermatogenesis as it is general or SC-specific KO. Using the Cre/loxP recombinase system, two conditional KO mouse lines lacking Cx43 in premeiotic (pGCCx43KO) or meiotic GC (mGCCx43KO) were generated. It was demonstrated by qRT-PCR that Cx43 mRNA was significantly decreased in adult pGCCx43KO mice, while it was also reduced in mGCCx43KO mice, yet not statistically significant. Body and testis weights, testicular histology, tubular diameter, numbers of intratubular cells and Cx43 protein synthesis and localization did not show any significant differences in semi-quantitative Western blot analysis and immunohistochemistry comparing adult male KO and WT mice of both mouse lines. Male KO mice were fertile. These results indicate that Cx43 in spermatogonia/spermatids does not seem to be essential for successful termination of spermatogenesis and fertility as it is known for Cx43 in somatic SC, but SC-GC communication might rather occur via heterotypic GJ channels.

ACS Style

Kristina Rode; Marion Langeheine; Bettina Seeger; Ralph Brehm. Connexin43 in Germ Cells Seems to Be Dispensable for Murine Spermatogenesis. International Journal of Molecular Sciences 2021, 22, 7924 .

AMA Style

Kristina Rode, Marion Langeheine, Bettina Seeger, Ralph Brehm. Connexin43 in Germ Cells Seems to Be Dispensable for Murine Spermatogenesis. International Journal of Molecular Sciences. 2021; 22 (15):7924.

Chicago/Turabian Style

Kristina Rode; Marion Langeheine; Bettina Seeger; Ralph Brehm. 2021. "Connexin43 in Germ Cells Seems to Be Dispensable for Murine Spermatogenesis." International Journal of Molecular Sciences 22, no. 15: 7924.

Journal article
Published: 19 September 2020 in Sustainability
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Rye could offer diverse benefits in terms of sustainability if it could replace parts of the main cereals, corn and wheat, in broiler diets. A total of 256 broilers, Ross 308, were randomly allocated into 32 pens. From day 14 till day 42, the birds were divided into four feeding groups (eight replicates each). The control group received a conventional finisher diet “control”, whereas in the other groups, a pelleted supplementary feed was offered (SFI to corn and SFII to rye), to which crushed corn (SFI-Corn) or squashed rye (SFII-Rye) was added. The fourth group received a mixture of 50% SFI-Corn and 50% SFII-Rye. The cereal level was increased weekly (5%, 10%, 20%, 30%) at the expense of the supplementary feeds. No significant effects were observed for body weight at d 42 and excreta viscosity between all groups. Overall, foot pad health was excellent. Compared to the control group, birds fed SFI-Corn displayed a significant increase in gizzard relative weight, whereas, in contrast to all other groups, ileal villus height was significantly lower. In conclusion, feeding SFI-Corn or SFII-Rye diets had no negative influences on performance, litter quality and digesta viscosity, whereas SFI-Corn partially affected ileal morphology.

ACS Style

Amr El-Wahab; Jan Lingens; Bussarakam Chuppava; Marwa Ahmed; Ahmed Osman; Marion Langeheine; Ralph Brehm; Venja Taube; Richard Grone; Andreas Von Felde; Josef Kamphues; Christian Visscher. Impact of Rye Inclusion in Diets for Broilers on Performance, Litter Quality, Foot Pad Health, Digesta Viscosity, Organ Traits and Intestinal Morphology. Sustainability 2020, 12, 7753 .

AMA Style

Amr El-Wahab, Jan Lingens, Bussarakam Chuppava, Marwa Ahmed, Ahmed Osman, Marion Langeheine, Ralph Brehm, Venja Taube, Richard Grone, Andreas Von Felde, Josef Kamphues, Christian Visscher. Impact of Rye Inclusion in Diets for Broilers on Performance, Litter Quality, Foot Pad Health, Digesta Viscosity, Organ Traits and Intestinal Morphology. Sustainability. 2020; 12 (18):7753.

Chicago/Turabian Style

Amr El-Wahab; Jan Lingens; Bussarakam Chuppava; Marwa Ahmed; Ahmed Osman; Marion Langeheine; Ralph Brehm; Venja Taube; Richard Grone; Andreas Von Felde; Josef Kamphues; Christian Visscher. 2020. "Impact of Rye Inclusion in Diets for Broilers on Performance, Litter Quality, Foot Pad Health, Digesta Viscosity, Organ Traits and Intestinal Morphology." Sustainability 12, no. 18: 7753.

Journal article
Published: 25 May 2020 in Animals
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The aim of this study was to test whether a single testicular needle biopsy could provide histological results comparable to en bloc resection histology and whether one biopsy was sufficient to reflect the histology of an entire pair of testicles. Two methods of sample collection were tested on 32 bull calves aged five to eight months to compare histological parameters of needle biopsy with those of en bloc resection samples. One testicular needle biopsy of the right and three en bloc samples of both testicles were collected and compared for the number of tubular cross sections, tubules with elongated spermatids (ES), outer/inner diameter of tubules, thickness of tubular wall, and number of Sertoli cells (SC). Additionally, animal data were considered. No significant differences were found between the left and right testis or among the individual locations of en bloc samples. However, histologically significant differences (Bonferroni-adjusted significance level: p < 0.05/6 = 0.0083) were found between the needle biopsy and en bloc resection regarding the tubular cross sections per visual field (p < 0.05), the outer (p = 0.01) and inner diameter and the thickness of the tubular wall (both p < 0.01). In the SOX9 immunohistochemical staining, no significant differences (p > 0.05) could be observed for SC numbers between needle biopsy and en bloc samples. In conclusion, results of testicular needle biopsy do not have the same validity as the en bloc resection histology. Furthermore, one biopsy is insufficient to reflect the histology of the entire testicular pair.

ACS Style

Maike Rohländer; Henning Otzen; Kristina Rode; Klaus Jung; Marion Schmicke; Teresa Harborth; Marion Langeheine; Ralph Brehm; Árpád Csaba Bajcsy. Histological Comparison of Testicular Needle Biopsy and En Bloc Samples in Abattoir Calves. Animals 2020, 10, 918 .

AMA Style

Maike Rohländer, Henning Otzen, Kristina Rode, Klaus Jung, Marion Schmicke, Teresa Harborth, Marion Langeheine, Ralph Brehm, Árpád Csaba Bajcsy. Histological Comparison of Testicular Needle Biopsy and En Bloc Samples in Abattoir Calves. Animals. 2020; 10 (5):918.

Chicago/Turabian Style

Maike Rohländer; Henning Otzen; Kristina Rode; Klaus Jung; Marion Schmicke; Teresa Harborth; Marion Langeheine; Ralph Brehm; Árpád Csaba Bajcsy. 2020. "Histological Comparison of Testicular Needle Biopsy and En Bloc Samples in Abattoir Calves." Animals 10, no. 5: 918.

Journal article
Published: 10 March 2020 in Cells
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Male factor infertility is a problem in today’s society but many underlying causes are still unknown. The generation of a conditional Sertoli cell (SC)-specific connexin 43 (Cx43) knockout mouse line (SCCx43KO) has provided a translational model. Expression of the gap junction protein Cx43 between adjacent SCs as well as between SCs and germ cells (GCs) is known to be essential for the initiation and maintenance of spermatogenesis in different species and men. Adult SCCx43KO males show altered spermatogenesis and are infertile. Thus, the present study aims to identify molecular mechanisms leading to testicular alterations in prepubertal SCCx43KO mice. Transcriptome analysis of 8-, 10- and 12-day-old mice was performed by next-generation sequencing (NGS). Additionally, candidate genes were examined by qRT-PCR and immunohistochemistry. NGS revealed many significantly differentially expressed genes in the SCCx43KO mice. For example, GC-specific genes were mostly downregulated and found to be involved in meiosis and spermatogonial differentiation (e.g., Dmrtb1, Sohlh1). In contrast, SC-specific genes implicated in SC maturation and proliferation were mostly upregulated (e.g., Amh, Fshr). In conclusion, Cx43 in SCs appears to be required for normal progression of the first wave of spermatogenesis, especially for the mitosis-meiosis switch, and also for the regulation of prepubertal SC maturation.

ACS Style

Erika Hilbold; Ottmar Distl; Martina Hoedemaker; Sandra Wilkening; Rüdiger Behr; Aleksandar Rajkovic; Marion Langeheine; Kristina Rode; Klaus Jung; Julia Metzger; Ralph H. J. Brehm. Loss of Cx43 in Murine Sertoli Cells Leads to Altered Prepubertal Sertoli Cell Maturation and Impairment of the Mitosis-Meiosis Switch. Cells 2020, 9, 676 .

AMA Style

Erika Hilbold, Ottmar Distl, Martina Hoedemaker, Sandra Wilkening, Rüdiger Behr, Aleksandar Rajkovic, Marion Langeheine, Kristina Rode, Klaus Jung, Julia Metzger, Ralph H. J. Brehm. Loss of Cx43 in Murine Sertoli Cells Leads to Altered Prepubertal Sertoli Cell Maturation and Impairment of the Mitosis-Meiosis Switch. Cells. 2020; 9 (3):676.

Chicago/Turabian Style

Erika Hilbold; Ottmar Distl; Martina Hoedemaker; Sandra Wilkening; Rüdiger Behr; Aleksandar Rajkovic; Marion Langeheine; Kristina Rode; Klaus Jung; Julia Metzger; Ralph H. J. Brehm. 2020. "Loss of Cx43 in Murine Sertoli Cells Leads to Altered Prepubertal Sertoli Cell Maturation and Impairment of the Mitosis-Meiosis Switch." Cells 9, no. 3: 676.

Journal article
Published: 19 November 2019 in Animals
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Sustainably produced poultry meat with consideration of animal health poses a challenge for broiler production. Low protein diets with high amounts of synthetic amino acids (AAs) like methionine (Met) are the consequence. In a five-week feeding trial, 360 broilers (Ross 308) assigned to four feeding groups were offered protein-reduced complete diets (starter: 20% crude protein (CP); grower: 18.5% CP; finisher: 17.5% CP), supplemented with essential AAs. The “MHA” group received DL-2-hydroxy-4-(methylthio) butanoic acid (DL-HMTBA; trade name: MHA®), groups “L” and “DL” the respective Met source in equivalent concentrations each exceeding the nutritional recommendations. “R-MHA” (“R” for “reduced”) received the minimum required level (using MHA as Met source). Performance exceeded performance goals without differences between the groups. The average feed conversion ratio (FCR) amounted to 1.35. The carcass/body weight ratio of R-MHA was significantly lower (0.782) compared to DL (0.808) and L (0.809). Breast meat of R-MHA contained significantly more fat (144 g/kg dry matter (DM)) compared to L (104 g/kg DM) and significantly lower CP content (R-MHA: 838 g/kg DM; L: 875 g/kg DM). The results indicated possible improvement in slaughter yield by protein-reduced diets supplemented with L-Met, thus recommending further research focusing on the Met influence.

ACS Style

Cristina Ullrich; Marion Langeheine; Ralph Brehm; Venja Taube; Mercedes Rosillo Galera; Karl Rohn; Johanna Popp; Christian Visscher. Influence of Different Methionine Sources on Performance and Slaughter Characteristics of Broilers. Animals 2019, 9, 984 .

AMA Style

Cristina Ullrich, Marion Langeheine, Ralph Brehm, Venja Taube, Mercedes Rosillo Galera, Karl Rohn, Johanna Popp, Christian Visscher. Influence of Different Methionine Sources on Performance and Slaughter Characteristics of Broilers. Animals. 2019; 9 (11):984.

Chicago/Turabian Style

Cristina Ullrich; Marion Langeheine; Ralph Brehm; Venja Taube; Mercedes Rosillo Galera; Karl Rohn; Johanna Popp; Christian Visscher. 2019. "Influence of Different Methionine Sources on Performance and Slaughter Characteristics of Broilers." Animals 9, no. 11: 984.

Journal article
Published: 23 October 2018 in Sustainability
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The current discussion concerning resource-efficient broiler production inevitably leads to diets with lowered crude protein (CP) levels. Therefore, the hypothesis was formed that crude protein reduction far below the recommended levels can significantly lower the nitrogen (N) content in litter, if essential amino acids are added and a constant lysine-arginine ratio is guaranteed. In a five-week feeding trial, 360 ROSS 308 broilers of both sexes were randomly assigned to four feeding groups with six replicates each with a standard three-phase feeding program (d 1–7, d 8–14, d 15–35). The control group was offered a complete diet with a common protein content found in practice (CP-% as fed; starter: 21.5, grower: 20.5, finisher: 20.0; lysine/arginine: 100/115). In the experimental diets the lysine/arginine ratio was constant, whereas the protein content was lowered in steps of 1.00 percent each with simultaneous supplementation of growth limiting amino acids. Feeding a diet with a 2.00 percent reduced protein content led to higher body weights after 34 days compared to the control (2329 g vs. 2192 g). The N content in the total litter decreased significantly with a 2.00 and 3.00 percent reduction in the CP content (51.2 vs. 46.2 or rather 36.2 g/kg dry matter (DM)). Meticulous balanced protein-reduced diets therefore allow a significant environmental relief.

ACS Style

Cristina Ullrich; Marion Langeheine; Ralph Brehm; Venja Taube; Diana Siebert; Christian Visscher. Influence of Reduced Protein Content in Complete Diets with a Consistent Arginine–Lysine Ratio on Performance and Nitrogen Excretion in Broilers. Sustainability 2018, 10, 3827 .

AMA Style

Cristina Ullrich, Marion Langeheine, Ralph Brehm, Venja Taube, Diana Siebert, Christian Visscher. Influence of Reduced Protein Content in Complete Diets with a Consistent Arginine–Lysine Ratio on Performance and Nitrogen Excretion in Broilers. Sustainability. 2018; 10 (11):3827.

Chicago/Turabian Style

Cristina Ullrich; Marion Langeheine; Ralph Brehm; Venja Taube; Diana Siebert; Christian Visscher. 2018. "Influence of Reduced Protein Content in Complete Diets with a Consistent Arginine–Lysine Ratio on Performance and Nitrogen Excretion in Broilers." Sustainability 10, no. 11: 3827.