Laying hens require less phosphorus (P) but markedly more calcium (Ca) in their diet than broilers. These differences may cause more distinct interactions with phytate degradation and utilization of minerals in laying hens than those in broilers. The objective of the study was to characterize intestinal phytate degradation, ileal transcript copy numbers of transcellular Ca and P transporters, and mineral utilization by two laying hen strains fed with standard or reduced levels of dietary Ca and P at the laying peak. The strains showed differences regarding several traits driving Ca and P metabolism along the digestive tract. Thus, the two strains may use different mechanisms to meet their respective P demand, i.e., via effective phytate degradation and transcellular transport. Clear effects of the Ca level on myo-inositol concentrations and mineral utilization revealed the significance of this element for the measured traits. The absence of P-mediated effects confirmed the findings of several studies recommending that P concentrations used in laying hen feeds are too high. Differences were noted between individuals within one treatment. The next step would be to evaluate the data in individual birds to identify birds that better cope with a challenging diet.

The objective of this contribution was to summarize from scientific literature the optimal concentration of nonphytate phosphorus (NPP) in feed for laying hens. The considered studies were one meta-analysis from 2012 and original studies published since then. Dietary treatments in the studies included variation in supplementation with mineral P sources and phytase. The studies investigated different periods of production and varied in duration but data were insufficient to analyze such factors in a systematic way. No study showed a positive effect on performance and eggshell when the NPP concentration was increased above 2.2 g NPP/kg of feed without the use of phytase. At such level, no consistent impairment of various bone quality traits were found but only few studies on bone quality traits were published. Overall, the data suggested that not more than 2.2 g NPP/kg of feed is needed for laying hens in different stages of production. This value can be reduced when phytase is added to the feed. Such reduction may differ depending on factors such as phytate content of the feed and phytase dosage. However, data are insufficient for calculating precise values of reduction. While phytate degradation in laying hens was markedly increased by phytase supplementation in several studies, effects of phytase supplementation on performance and bone traits in laying hens were less conclusive probably because the hens were supplied more than their NPP requirement. Transition to a system based on digestible P for laying hens similar to broiler chickens may support more precise P nutrition and more sustainable egg production in the future.

Insufficient nutrient supply can impair the immune system, which is important for animal health and welfare. Since chicken can partly hydrolyze phytate, which is the primary phosphorus storage in plant seeds, a reduction of mineral phosphorus in the diets could be an option for more sustainable egg production. Laying hens require high concentrations of calcium that might inhibit the function of endogenous enzymes for phytate hydrolyzation. The objective of this study was to characterize the impact of standard and reduced dietary phosphorus and calcium concentrations on the number and functionality of immune cells in the peripheral and gut-associated immune system in a white and brown laying hen strain. Reduced mineral phosphorus enhanced several immune parameters such as B cells in blood and IgA concentrations in bile in both strains, and peripheral monocytes and T cells in cecal tonsils in brown hens. Reduced calcium levels resulted in lower numbers of T cells in blood and cecal tonsils in both strains, suggesting negative effects on adaptive immunity. Differences between the two strains were found in almost all immune parameters. Results suggest a potentially beneficial effect of reduced dietary mineral phosphorus on the immune system that is dependent on the genetic background.

Phytase supplementation is gaining importance in animal nutrition because of its effect on phosphorus (P) digestibility and the increasing relevance of P for sustainable production. The potential inhibitors of phytase efficacy and phytate degradation, such as calcium (Ca) and zinc (Zn), have been a subject of intense research. This review focuses on the interactions of Zn with phytate and phytase in the digestive tract of poultry and pigs, with an emphasis on the effects of Zn supplementation on phytase efficacy and P digestibility. In vitro studies have shown the inhibitory effect of Zn on phytase efficacy. However, relevant in vivo studies are scarce and do not show consistent results for poultry and pigs. The results could be influenced by different factors, such as diet composition, amount of Zn supplement, mineral concentrations, and phytase supplementation, which limit the comparability of studies. The chosen response criteria to measure phytase efficacy, which is mainly tibia ash, could also influence the results. Compared to poultry, the literature findings are somewhat more conclusive in pigs, where pharmacological Zn doses (≥ 1000 mg kg⁻¹ Zn) appear to reduce P digestibility. To appropriately evaluate the effects of non‐pharmacological Zn doses, further studies are needed that provide comprehensive information on their experimental setup and include measurements of gastrointestinal phytate degradation to better understand the mechanisms associated with Zn and phytase supplements. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

The objective of this study was to characterize intestinal phytate degradation and mineral utilization by 2 laying hen strains before and after the onset of egg laying using diets without or with a mineral phosphorus (P) supplement. One offspring of 10 roosters per strain (Lohmann Brown-classic [LB] and Lohmann LSL-classic [LSL]) was sacrificed before (wk 19) and after (wk 24) the onset of egg-laying activity and following 4 wk placement in a metabolic unit. Diets were corn-soybean meal-based and without supplemented P (P-) or with 1 g/kg supplemented P (P+) from monocalcium phosphate. In wk 19 and 24, the blood plasma and digesta of duodenum+jejunum and distal ileum were collected. The concentration of P in blood plasma was higher in hens fed P+ than P- (P < 0.001). In duodenum + jejunum and ileum content, the concentrations of InsP₆, Ins(1,2,4,5,6)P₅ and Ins(1,2,3,4,5)P₅ were lower in P- than in P+ (P ≤ 0.009). In duodenum+jejunum, the concentrations of InsP₆, Ins(1,2,4,5,6)P₅ and Ins(1,2,3,4,5)P₅ were lower in wk 24 than 19 and lower in LSL than LB hens (P < 0.001). The concentration of myo-inositol (MI) in duodenum + jejunum content was lower in wk 19 than 24 (P < 0.001). Following a 4-d total excreta collection, the retained amount of P was higher in P+ than P- (P < 0.001). Phosphorus retention was lower in LB hens fed P- than in other treatments (P × strain: P = 0.039). In the jejunal tissue, some genes related to intracellular InsP metabolism were higher expressed in LB than LSL hens. The renunciation of mineral P increased endogenous phytate degradation, but more P was retained with supplemented P. Differences in endogenous phytate degradation between the periods before and after the onset of egg laying might be attributed to different Ca concentrations in intestinal digesta caused by different Ca needs in both periods.

A comparison between 3-wk-old female turkeys (B.U.T. 6) and broilers (Ross 308) was performed to study the effects of species, dietary P, Ca, and phytase levels on gut mucosal phosphatase activity, myo-inositol hexakisphosphate (InsP₆) degradation along the digestive tract, digestibility of P, Ca, and amino acids, and concentrations of myo-inositol in the digesta and blood. The experimental diets were corn-soybean meal-based and identical for both species. Two dietary P and Ca concentrations (CaP-: 4.1 g P/kg, 5.5 g Ca/kg and CaP+: 9.0 g P/kg, 12.0 g Ca/kg) and 2 levels of phytase supplementation (0 and 1,500 FTU/kg) were used in a 2 × 2 factorial design and fed to the animals for 7 d in their third week of age. Each diet was randomly assigned to 6 broiler and 6 turkey pens, with 10 birds each. After slaughter, blood, digesta from the crop, gizzard, duodenum, lower ileum, and mucosa from the jejunum were collected. When fed CaP- without phytase supplementation, there were no differences between species in gut mucosal phosphatase activity, prececal InsP₆ disappearance, and P and Ca digestibility, indicating a similar intrinsic capacity for phytate degradation in both species. When fed CaP+ without phytase supplementation, turkeys showed higher prececal InsP₆ disappearance than broilers. Phytase supplementation increased prececal InsP₆ disappearance and digestibility of P and Ca in both species. However, the phytase-induced increase in prececal InsP₆ disappearance was more pronounced in broilers than in turkeys, possibly due to more adequate conditions for phytase activity in the broiler crop. In broilers, phytase supplementation increased amino acid digestibility overall, whereas, in turkeys, it increased with CaP+ and decreased with CaP-. In addition, the relationship between myo-inositol concentration in the ileum and blood differed between species, indicating differences in myo-inositol metabolism. It was concluded that 3-week-old turkeys and broilers differ in nutrient digestibility and InsP degradation in some segments of the digestive tract but have similar endogenous InsP₆ degradation when fed low P and Ca diets.