Pretreatment of lignocellulosic biomass (LCB) is a key step for its efficient bioconversion into ethanol. Determining the best pretreatment and its parameters requires monitoring its impacts on the biomass material. Here, we used fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay to study the relationship between surface-exposed polysaccharides and enzymatic hydrolysis of LCB. Our results indicated that alkali extrusion pretreatment led to the highest hydrolysis rates for alfalfa stover, cattail stems and flax shives, despite its lower lignin removal efficiency compared to alkali pretreatment. Corn crop residues were more sensitive to alkali pretreatments, leading to higher hydrolysis rates. A clear relationship was consistently observed between total surface-exposed cellulose detected by the FTCM-depletion assay and biomass enzymatic hydrolysis. Comparison of bioconversion yield and total composition analysis (by NREL/TP-510-42618) of LCB prior to or after pretreatments did not show any close relationship. Lignin removal efficiency and total cellulose content (by NREL/TP-510-42618) led to an unreliable prediction of enzymatic polysaccharide hydrolysis. Fluorescent protein-tagged carbohydrate-binding modules method (FTCM)-depletion assay provided direct evidence that cellulose exposure is the key determinant of hydrolysis yield. The clear and robust relationships that were observed between the cellulose accessibility by FTCM probes and enzymatic hydrolysis rates change could be evolved into a powerful prediction tool that might help develop optimal biomass pretreatment strategies for biofuel production.

Forest bioenergy value chains can offer attractive opportunities to promote economic development and mitigate climate change. However, implementing profitable and efficient forest biomass value chains requires overcoming barriers that continue to hinder the development of bioenergy systems in several jurisdictions. The objective of this study was to compare the economic, social, and environmental sustainability of various potential configurations of forest bioenergy value chains, including forest biomass supply and bioenergy production chains, in the Capitale-Nationale region of Quebec (Canada), which is a jurisdiction that has considerable forest resources but makes little use of bioenergy. We based our study on the ToSIA model parameterization and compared various policy measures, biomass supply, and logistics scenarios for 2008 and 2030. Our results showed that wood chip and pellet value chains in the Capitale-Nationale region would positively contribute to the regional economy in 2030, even in the absence of subsidies. Moreover, actions to increase biomass feedstock mobilization in 2030 would lead to an increase in gross value added, employment, and energy production in the region compared with 2008 and a greater increase than other considered policy or logistical measures. However, increased biomass feedstock mobilization would also mean higher relative GHG emissions and more fossil fuel energy input per unit of bioenergy than in the other scenarios. Conversely, optimizing biomass feedstock and combustion technologies could help minimize the fossil fuel energy input needed and GHG and some non-GHG pollutant emissions. Overall, our study suggested that implementing policy and logistical measures for forest biomass value chains could make the significant mobilization of forest bioenergy attainable and, in turn, Quebec’s 2030 bioenergy target of 17 petajoules realistic.

Wood plastic composite (WPC) usage and demand have increased because of its interesting chemical and mechanical properties compared to other plastic materials. However, there is a possibility of structural and mechanical changes to the material when exposed to the external environment; most research on wood plastic is performed on the material with elevated fiber content (40–70%). Therefore, more research needs to be performed regarding these issues, especially when the fiber content of the WPC is low. In this study, composite materials composed of high-density polyethylene (HDPE) reinforced with yellow birch fibers (20 and 30%) were made by injection molding. The fibers were treated with dissolved zinc oxide (ZnO) powder in sodium oxide (NaOH) solution, and the fabricated material was exposed to fungal rot. ZnO treatment in this case is different from most studies because ZnO nanoparticles are usually employed. The main reason was to obtain better fixation of ZnO on the fibers. The mechanical properties of the composites were assessed by the tensile and Izod impact tests. The impact energies of the samples fabricated with ZnO-treated fibers and exposed to Gloephyllum trabeum and Trametes versicolor decreased, when compared to samples fabricated with ZnO-nontreated fibers. The mechanical properties of the samples composed of ZnO-treated fibers and exposed to rot decreased, which were reported by a decreased Young’s modulus and impact energies. The usage of ZnO treatment prevented mycelium proliferation, which was nonexistent on the samples. It has been noted that the decrease in mechanical properties of the treated samples was because of the action of NaOH used to dissolve the ZnO powder.