In the modern agricultural landscape, numerous challenges, such as climate change, diminishing arable lands, and the reduction of water resources, represent significant threats. The Mediterranean greenhouse farming model relies on low-input strategies to maximize both yield and quality. Its protected horticulture is essential for the year-round cultivation of high-value crops, ensuring efficient and sustainable production. In the realm of future agricultural strategies, leveraging internet-based approaches emerges as a pivotal factor for real-time and remote control of various agricultural parameters crucial for crop growth and development. This approach has the potential to significantly optimize agronomic inputs, thereby enhancing the efficiency of targeted vegetable production. The aim of the present review is to underscore the challenges related to the intensive greenhouse production systems emphasizing various strategies leading to low-input greenhouse vegetable production. The goal is to promote more sustainable and resource-efficient approaches in the cultivation of greenhouse vegetables. This review highlights several key strategies for optimizing the greenhouse environment, including efficient water management through conservation tillage, drainage water reuse, and selecting the most appropriate irrigation systems and timing. Additionally, light modulation and temperature control—using solar energy for heating and pad-and-fan systems for cooling—are crucial for enhancing both crop performance and resource efficiency. The review also explores low-input agronomical strategies, such as pest and disease control—including solarization and optimized integrated pest management (IPM)—as well as fertilization and advanced growing techniques. These approaches are essential for sustainable greenhouse farming.

Since 2007, the European Commission AGRI GEN RES 018 “CROCUSBANK” action has permitted the creation of the alleged World Saffron and Crocus Collection (WSCC), a unique collection which contains a representation of the genetic variability present in saffron crop and wild relatives at global scale. At present the germplasm collection, housed at the Bank of Plant Germplasm of Cuenca (BGV-CU, Spain), consists of 572 preserved accessions representing 47 different Crocus species (including saffron Crocus) and is expected to increase up to more than 600 accessions by the end of CROCUSBANK action (May 2011). The preserved biodiversity of saffron (Crocus sativus L.) covers a wide range of the genetic variability of the crop and currently consists of 220 accessions from 15 countries: 169 of these come from European cultivation countries, 18 from commercial areas in non EU countries, 26 from regions of minimal or relict production and/or from abandoned fields and 7 from commercial nurseries. The non-saffron Crocus collection currently comprises 352 accessions: 179 collected from the wild in 12 countries of natural distribution, 24 from donations of public and private institutions, 91 from commercial nurseries and 58 acquired from BGV-CU collection management. Here we provide a record of collections, activities concerns and current strategies for documentation, conservation, characterisation, and management of the collection as important tools for researchers with interest in these valuable genetic resources.

Tomato crops are affected in Mediterranean cold-greenhouse agrosystems by soilborne diseases, such as root-knot nematodes (Meloidogyne spp.), which represent a serious problem leading to losses in production. Agroecological soil management based on biocontrol agents and natural compounds has had increased grower interest in order to reduce chemical residues in the produce and to adopt environmentally friendly farming methods. In this frame, we evaluate and validate soil biofumigation by the use of glucosinolate (GLS) compounds. Among them, sinigrin showed biocontrol activities against several pests and diseases via nematotoxic action. Among the Brassicaceae species rich in sinigrin, we chose Brassica macrocarpa Guss. (BM) because its leaves show 90% of all GLSs, and we could better estimate the action of this single GLS. Different dosages of BM leaf flour, containing 200 to 300, 350, 400, 450, and 650 μmol m⁻² of sinigrin, were inserted into soil already infected by Meloidogyne spp. for evaluating their effects on tomatoes grown in cold greenhouses in comparison to absolute control (CTRL) and to the chemical one, Vydate 5G^® (CCTRL). The root disease index, caused by nematode attack, was the highest in CTRL, and a reduction of about 50% was observed with the 300 to 650 μmol m⁻² sinigrin dosage. The CCTRL showed twofold marketable yield increase, and a fourfold increase was found in 650 μmol m⁻² of sinigrin dosage, in comparison to the CTRL. Biofumigant applications improved tomato plant growth and development, and fruit quality, significantly for dry matter and soluble sugars (°Brix). BM leaf flour inserted into the soil, at a dose of 300 μmol m⁻² of sinigrin, showed similar effects to the CCTRL on root disease index, root weight, and marketable yield. Data showed the nematotoxic effect of sinigrin for the biocontrol of Meloydogine spp. by the use of B. macrocarpa leaves, very rich in this GLS compound, which represents a new tool for agroecological soil management and for organic farming.

The rheological and chemical quality of pasta samples, which were obtained using the durum wheat semolina fortified with the hemp seed solid residue, after oil extraction, sieved at 530 μm (Hemp 1) or 236 μm (Hemp 2) at different percentages of substitution (5%, 7.5%, and 10%, were evaluated. The total polyphenolic content in hemp flour was quantified in the range of 6.38–6.35 mg GAE/g, and free radical scavenging was included in the range from 3.94–3.75 mmol TEAC/100 g in Hemp 1 and Hemp 2, respectively. The phenolic profiles determined by UHPLC-ESI/QTOF-MS showed that cannabisin C, hydroxycinnamic and protocatechuic acids were the most abundant phenolic compounds in both hemp flours. Among the amino acids, isoleucine, glutamine, tyrosine, proline, and lysine were the most abundant in raw materials and pasta samples. Although the hemp seeds were previously subjected to oil extraction, hemp flours retain about 8% of oil, and the fatty acids present in the largest amount were linoleic acid and α-linolenic acid. Characterization of the minerals showed that the concentration of macro and trace elements increased according to fortification percentage. Sensory evaluation and cooking quality indicated that the best performance in terms of process production and consumer acceptance was obtained using Hemp 2 at 7.5%. Hemp supplementation could be a potential option for producing high-quality, nutritionally rich, low-cost pasta with good color and functionality.

In the present study, we evaluated for the first time the variability of antioxidant traits of four Brassica wild species: B. incana, B. macrocarpa, B. villosa, and B. rupestris. The content of the main water-soluble antioxidants (phenolics, ascorbic acid, and total biothiols) and the in vitro antioxidant potential (1,1-diphenyl-2-picrylhydrazil (DPPH) and superoxide anion scavenging capacity) were investigated. A total of 28 polyphenolic compounds were identified by LC/MS and quantitated by HPLC/DAD analysis. Kaempferol and quercetin derivatives were the most abundant phenolics compared to hydroxycinnamoyl gentiobiosides. In the ten populations, phenolics ranged from 163.9 to 533.9 mg/100 g dry weight (d.w.), ascorbic acid from 7.6 to 375.8 mg/100 g d.w., and total biothiols from 0.59 to 5.13 mg/100 g d.w. The different classes of phytochemicals were separated using solid-phase extraction at increasing methanol concentrations, and the antioxidant power of fractionated extracts was evaluated. The superoxide anion scavenging activity was significantly correlated to phenolics, particularly to flavonol derivatives, while DPPH was mainly related to ascorbic acid content. The present findings improve the knowledge of the phytochemical composition of Italian Brassica wild species by showing the great diversity of phytochemicals among populations and highlighting their importance as a valuable genetic resource for developing new cultivars with improved bioactive content.