Nutraceutical combinations that act synergistically could be a powerful solution against colon cancer, which is the second deadliest malignancy worldwide. In this study, curcumin (C), sulforaphane (S), and dihydrocaffeic acid (D, a chlorogenic acid metabolite) were evaluated, individually and in different combinations, over the viability of HT-29 and Caco-2 colon cancer cells, and compared against healthy fetal human colon (FHC) cells. The cytotoxic concentrations to kill 50%, 75%, and 90% of the cells (CC₅₀, CC₇₅, and CC₉₀) were obtained, using the MTS assay. Synergistic, additive, and antagonistic effects were determined by using the combination index (CI) method. The 1:1 combination of S and D exerted synergistic effects against HT-29 at 90% cytotoxicity level (doses 90:90 µM), whereas CD(1:4) was synergistic at all cytotoxicity levels (9:36–34:136 µM) and CD(9:2) at 90% (108:24 µM) against Caco-2 cells. SD(1:1) was significantly more cytotoxic for cancer cells than healthy cells, while CD(1:4) and CD(9:2) were similarly or more cytotoxic for healthy cells. Therefore, the SD(1:1) combination was chosen as the best. A model explaining SD(1:1) synergy is proposed. SD(1:1) can be used as a basis to develop advanced food products for the prevention/co-treatment of colon cancer.

This research focuses on developing a novel ultra high molecular weight polyethylene (UHMWPE) material reinforced with titanium dioxide (TiO ${}_2$ ) nanoparticles for producing craniofacial prostheses via an incremental sheet forming process (SPIF). First, UHMWPE-TiO ${}_2$ nanocomposite sheets were produced using incipient wetting and the compression molding process by considering different concentrations of TiO ${}_2$ nanoparticles. Then, the influence that the compression molding fabrication process has on the crystallinity and structural properties of the produced sample sheets was investigated. Experimental characterizations via scanning electron microscopy (SEM), differential scanning calorimetry (DSC), X-ray diffraction (XRD), Fourier transform infrared (FT-IR), tensile mechanical testing, and live/dead cell viability assays provided data that show an enhancement of the physical, mechanical, and biological properties. Finally, modifications on the nanocomposite material properties due to the SPIF manufacturing processes of a craniofacial prosthesis are addressed.