The removal characteristics of antibiotic resistance genes and mobile genetic elements from livestock wastewater (dominated by quinolone antibiotics) treated with MFC were evaluated by High-throughput quantitative (HT-qPCR). The results showed that 144 ARGs and 8 MEGs were detected in the livestock wastewater. After MFC treatment, the number of AGRs decreased as a whole, and the relative abundance of macrolide-lincosamide-streptogramin group B (MLSB) and aminoglycosider decreased by 62.7% and 92.9%, respectively. MGEs decreased by 57.3% and multidrug genes decreased by 90%. After MFC treatment, the absolute abundance of tetracycline in raw sewage decreased by two orders of magnitude from 5.8 × 105 copies L-1 to 5.1.× 103 copies L-1. However, MFC was less efficient in the removal of vancomycin and beta-lactamase genes. It was also found that chloramphenicol resistance genes slightly increased. Illumina sequencing showed that Syntrophobacterales and Synergistales were predominant in MFCs. Desulfovibrio was resistant to high concentration of moxifloxacin hydrochloride. The removal efficiency of MFC for moxifloxacin hydrochloride at a concentration of 5 mg L-1 was 86.55%. The maximum power density and coulomb efficiency were 109.3 mV·cm-3 and 41.97%, respectively. With the increase of antibiotic concentration, the sewage treatment efficiency and electrical performance were inhibited. This study shows that untreated livestock wastewater had a great risk of gene horizontal transfer. Although MFC had limited treatment capacity for high-concentration quinolone wastewater, it is an effective method to reduce ARGs and the risk of horizontal gene transfer.

With the growth of the global population and the acceleration of industrialization, the problem of water pollution has become increasingly serious, posing a major threat to the ecosystem and human health. Traditional water treatment technologies make it difficult to cope with complex pollution, so the scientific community is actively exploring new and efficient treatment methods. Biochar (BC), as a low-cost, green carbon-based material, exhibits good adsorption and catalytic properties in water treatment due to its porous structure and abundant active functional groups. However, BC’s pure adsorption or catalytic capacity is limited, and researchers have dramatically enhanced its performance through modification means, such as loading metals or heteroatoms. In this paper, we systematically review the recent applications of BC and its modified materials for water treatment in adsorption, Fenton-like, electrocatalytic, photocatalytic, and sonocatalytic systems, and discuss their adsorption/catalytic mechanisms. However, most of the research in this field is at the laboratory simulation stage and still needs much improvement before it can be applied in large-scale wastewater treatment. This review improves the understanding of the pollutant adsorption/catalytic properties and mechanisms of BC-based materials, analyzes the limitations of the current studies, and investigates future directions.