This page has only limited features, please log in for full access.
In the current study, Achillea santolinoides and Achillea aleppica aeral parts and root were extracted with ethyl acetate, methanol, and water. Detailed phytochemical profiles were obtained using UHPLC-MS, yielding the identification of hydroxybenzoic and hydroxycinnamic acids, phenolic acid glycosides and sugar esters, acylquinic acids, O-glycosyl flavones and flavonols, and flavonoid aglycons, among others. The antioxidant properties and enzyme inhibitory activities of the extracts were assayed with in vitro tests. The phenolic content of the water extracts was significantly higher as compared to the ethyl acetate and methanol ones. A. aleppica aerial parts methanol extract possessed highest flavonoid content (49.18 mg rutin equivalent/g). Antioxidant properties assessment revealed that the methanol extract of A. santolinoides roots actively scavenged DPPH (54.11 mg TE/g) and ABTS radicals (112.53 mg TE/g) and possessed highest reducing potential (183.55 and 129.92 mg TE/g, for CUPRAC and FRAP, respectively). The ethyl acetate extracts of aerial parts and roots of both species showed highest inhibition against BuCHE (6.07–6.76 mg GALAE/g). The ethyl acetate extract of A.santolinoides aerial part showed highest inhibition against tyrosinase (73.00 mg KAE/g). These results showed that the tested Achillea species might represent novel phytotherapeutic avenues for the management of Alzheimer’s disease and epidermal hyperpigmentation conditions, which are both associated with oxidative stress. This paper could shed light into future potential industrial applications using the tested Achillea species.
Reneta Gevrenova; Gokhan Zengin; Kouadio Ibrahime Sinan; Evren Yıldıztugay; Dimitrina Zheleva-Dimitrova; Carene Picot-Allain; Mohamad Fawzi Mahomoodally; Muhammad Imran; Stefano Dall’Acqua. UHPLC-MS Characterization and Biological Insights of Different Solvent Extracts of Two Achillea Species (A. aleppica and A. santolinoides) from Turkey. Antioxidants 2021, 10, 1180 .
AMA StyleReneta Gevrenova, Gokhan Zengin, Kouadio Ibrahime Sinan, Evren Yıldıztugay, Dimitrina Zheleva-Dimitrova, Carene Picot-Allain, Mohamad Fawzi Mahomoodally, Muhammad Imran, Stefano Dall’Acqua. UHPLC-MS Characterization and Biological Insights of Different Solvent Extracts of Two Achillea Species (A. aleppica and A. santolinoides) from Turkey. Antioxidants. 2021; 10 (8):1180.
Chicago/Turabian StyleReneta Gevrenova; Gokhan Zengin; Kouadio Ibrahime Sinan; Evren Yıldıztugay; Dimitrina Zheleva-Dimitrova; Carene Picot-Allain; Mohamad Fawzi Mahomoodally; Muhammad Imran; Stefano Dall’Acqua. 2021. "UHPLC-MS Characterization and Biological Insights of Different Solvent Extracts of Two Achillea Species (A. aleppica and A. santolinoides) from Turkey." Antioxidants 10, no. 8: 1180.
The quest for sustainable strategies aimed at increasing the bioactive properties of plant-based foods has grown quickly. In this work, we investigated the impact of exogenously applied phenolics, i.e., chlorogenic acid (CGA), hesperidin (HES), and their combinations (HES + CGA), on Lactuca sativa L. grown under normal- and mild-salinity conditions. To this aim, the phenolic profile, antioxidant properties, and enzyme inhibitory activity were determined. The untargeted metabolomics profiling revealed that lettuce treated with CGA under non-stressed conditions exhibited the highest total phenolic content (35.98 mg Eq./g). Lettuce samples grown under salt stress showed lower phenolic contents, except for lettuce treated with HES or HES + CGA, when comparing the same treatment between the two conditions. Furthermore, the antioxidant capacity was investigated through DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,20-azinobis-(3-ethylbenzothiazoline-6-sulfonate)), and FRAP (ferric reducing antioxidant power) assays, coupled with metal-chelating activity and phosphomolybdenum capacity. An exciting increase in radical scavenging capacity was observed in lettuce treated with exogenous phenolics, in both stress and non-stress conditions. The inhibitory activity of the samples was evaluated against target health-related enzymes, namely cholinesterases (acetylcholinesterase; AChE; butyryl cholinesterase; BChE), tyrosinase, α-amylase, and α-glucosidase. Lettuce treated with HES + CGA under non-stress conditions exhibited the strongest inhibition against AChE and BChE, while the same treatment under salinity conditions resulted in the highest inhibition capacity against α-amylase. Additionally, CGA under non-stress conditions exhibited the best inhibitory effect against tyrosinase. All the functional traits investigated were significantly modulated by exogenous phenolics, salinity, and their combination. In more detail, flavonoids, lignans, and stilbenes were the most affected phenolics, whereas glycosidase enzymes and tyrosinase activity were the most affected among enzyme assays. In conclusion, the exogenous application of phenolics to lettuce represents an effective and green strategy to effectively modulate the phenolic profile, antioxidant activity, and enzyme inhibitory effects in lettuce, deserving future application to produce functional plant-based foods in a sustainable way.
Leilei Zhang; Erika Martinelli; Biancamaria Senizza; Begoña Miras-Moreno; Evren Yildiztugay; Busra Arikan; Fevzi Elbasan; Gunes Ak; Melike Balci; Gokhan Zengin; Youssef Rouphael; Luigi Lucini. The Combination of Mild Salinity Conditions and Exogenously Applied Phenolics Modulates Functional Traits in Lettuce. Plants 2021, 10, 1457 .
AMA StyleLeilei Zhang, Erika Martinelli, Biancamaria Senizza, Begoña Miras-Moreno, Evren Yildiztugay, Busra Arikan, Fevzi Elbasan, Gunes Ak, Melike Balci, Gokhan Zengin, Youssef Rouphael, Luigi Lucini. The Combination of Mild Salinity Conditions and Exogenously Applied Phenolics Modulates Functional Traits in Lettuce. Plants. 2021; 10 (7):1457.
Chicago/Turabian StyleLeilei Zhang; Erika Martinelli; Biancamaria Senizza; Begoña Miras-Moreno; Evren Yildiztugay; Busra Arikan; Fevzi Elbasan; Gunes Ak; Melike Balci; Gokhan Zengin; Youssef Rouphael; Luigi Lucini. 2021. "The Combination of Mild Salinity Conditions and Exogenously Applied Phenolics Modulates Functional Traits in Lettuce." Plants 10, no. 7: 1457.
The presented results regarding Limonium species could provide valuable information for a large field of industrial applications, including pharmaceutical and cosmeceutical formulations.
Biancamaria Senizza; Leilei Zhang; Gabriele Rocchetti; Gokhan Zengin; Gunes Ak; Evren Yıldıztugay; Fevzi Elbasan; Sharmeen Jugreet; Mohamad Fawzi Mahomoodally; Luigi Lucini. Metabolomic profiling and biological properties of six Limonium species: novel perspectives for nutraceutical purposes. Food & Function 2021, 12, 3443 -3454.
AMA StyleBiancamaria Senizza, Leilei Zhang, Gabriele Rocchetti, Gokhan Zengin, Gunes Ak, Evren Yıldıztugay, Fevzi Elbasan, Sharmeen Jugreet, Mohamad Fawzi Mahomoodally, Luigi Lucini. Metabolomic profiling and biological properties of six Limonium species: novel perspectives for nutraceutical purposes. Food & Function. 2021; 12 (8):3443-3454.
Chicago/Turabian StyleBiancamaria Senizza; Leilei Zhang; Gabriele Rocchetti; Gokhan Zengin; Gunes Ak; Evren Yıldıztugay; Fevzi Elbasan; Sharmeen Jugreet; Mohamad Fawzi Mahomoodally; Luigi Lucini. 2021. "Metabolomic profiling and biological properties of six Limonium species: novel perspectives for nutraceutical purposes." Food & Function 12, no. 8: 3443-3454.
Our findings suggested that Salvia ceratophylla could be one potential raw material in industrial applications.
Sengul Uysal; Gokhan Zengin; Kouadio Ibrahime Sinan; Gunes Ak; Ramazan Ceylan; Mohamad Fawzi Mahomoodally; Ahmet Uysal; Nabeelah Bibi Sadeer; József Jekő; Zoltán Cziáky; Maria João Rodrigues; Evren Yıldıztugay; Fevzi Elbasan; Luisa Custodio. Chemical characterization, cytotoxic, antioxidant, antimicrobial, and enzyme inhibitory effects of different extracts from one sage (Salvia ceratophylla L.) from Turkey: open a new window on industrial purposes. RSC Advances 2021, 11, 5295 -5310.
AMA StyleSengul Uysal, Gokhan Zengin, Kouadio Ibrahime Sinan, Gunes Ak, Ramazan Ceylan, Mohamad Fawzi Mahomoodally, Ahmet Uysal, Nabeelah Bibi Sadeer, József Jekő, Zoltán Cziáky, Maria João Rodrigues, Evren Yıldıztugay, Fevzi Elbasan, Luisa Custodio. Chemical characterization, cytotoxic, antioxidant, antimicrobial, and enzyme inhibitory effects of different extracts from one sage (Salvia ceratophylla L.) from Turkey: open a new window on industrial purposes. RSC Advances. 2021; 11 (10):5295-5310.
Chicago/Turabian StyleSengul Uysal; Gokhan Zengin; Kouadio Ibrahime Sinan; Gunes Ak; Ramazan Ceylan; Mohamad Fawzi Mahomoodally; Ahmet Uysal; Nabeelah Bibi Sadeer; József Jekő; Zoltán Cziáky; Maria João Rodrigues; Evren Yıldıztugay; Fevzi Elbasan; Luisa Custodio. 2021. "Chemical characterization, cytotoxic, antioxidant, antimicrobial, and enzyme inhibitory effects of different extracts from one sage (Salvia ceratophylla L.) from Turkey: open a new window on industrial purposes." RSC Advances 11, no. 10: 5295-5310.
The present study was conducted to uncover underlying possible effect mechanisms of flavonoid naringenin (Nar, 0.1–0.4 mM) in nitrogen assimilation, antioxidant response, redox status and the expression of NLP7 and DREB2A, on salt (100 mM NaCl) and osmotic-stressed (10% Polyethylene glycol, −0.54 MPa) Phaseolus vulgaris cv. Yunus 90). Nar ameliorated salt/osmotic stresses-induced growth inhibition and improved the accumulation of proline, glycine betaine and choline. In response to stress, Nar increased endogenous content of nitrate (NO3−) and nitrite (NO2−) by regulating of nitrate reductase and nitrite reductase. Stress-triggered NH4+ was eliminated with Nar through increases in glutamine synthetase and glutamate synthase. After NaCl or NaCl + PEG exposure, Nar utilized the aminating activity of glutamate dehydrogenase in the conversion of NH4+. The stress-inducible expression levels of DREB2A were increased further by Nar, which might have affected stress tolerance of bean. Nar induced effectively the relative expression of NLP7 in the presence of the combination or alone of stress. Also, the impaired redox state by stress was modulated by Nar and hydrogen peroxide (H2O2) and TBARS decreased. Nar regulated the different pathways for scavenging of H2O2 under NaCl and/or PEG treatments. When Nar + NaCl exposure, the damage was removed by superoxide dismutase (SOD), catalase (CAT), POX (only at 0.1 mM Nar + NaCl) and AsA-GSH cycle. Under osmotic stress plus Nar, the protection was manifested by activated CAT and, glutathione S-transferase and the regeneration of ascorbate. 0.1 mM Nar could protect bean plant against salt/osmotic stresses, likely by regulating nitrogen assimilation pathways, improving expression levels of genes associated with tolerance mechanisms and modulating the antioxidant capacity and AsA-GSH redox-based systems.
Ceyda Ozfidan-Konakci; Evren Yildiztugay; Fatma Nur Alp; Mustafa Kucukoduk; Ismail Turkan. Naringenin induces tolerance to salt/osmotic stress through the regulation of nitrogen metabolism, cellular redox and ROS scavenging capacity in bean plants. Plant Physiology and Biochemistry 2020, 157, 264 -275.
AMA StyleCeyda Ozfidan-Konakci, Evren Yildiztugay, Fatma Nur Alp, Mustafa Kucukoduk, Ismail Turkan. Naringenin induces tolerance to salt/osmotic stress through the regulation of nitrogen metabolism, cellular redox and ROS scavenging capacity in bean plants. Plant Physiology and Biochemistry. 2020; 157 ():264-275.
Chicago/Turabian StyleCeyda Ozfidan-Konakci; Evren Yildiztugay; Fatma Nur Alp; Mustafa Kucukoduk; Ismail Turkan. 2020. "Naringenin induces tolerance to salt/osmotic stress through the regulation of nitrogen metabolism, cellular redox and ROS scavenging capacity in bean plants." Plant Physiology and Biochemistry 157, no. : 264-275.
The genus Scorzonera contains important plants as traditional drugs and foods. In this sense, the aim of the present study was to determine the chemical composition, antioxidant activity and enzyme inhibitory effects of the root and aerial part of Scorzonera hispanica L. (S. hispanica) extracts. The antioxidant activities were evaluated using ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), metal chelating and phosphomolybdenum assays and the enzyme inhibitory properties were assessed against acetyl- (AChE) and butyryl-cholinesterase (BChE), tyrosinase, α-amylase and α-glucosidase. The results showed that the methanolic and ethyl acetate extracts possessed the highest phenol and flavonoid contents. The methanolic aerial part extract represented the highest antioxidant properties (FRAP: 58.41±1.55; CUPRAC: 126.18±0.94; DPPH: 47.92±0.07; ABTS: 71.69±0.03 mg Trolox equivalent (TE)/g) compared to the root extracts. The root extract significantly depressed AChE (2.64±0.02 mg galantamine equivalent (GALAE)/g), BChE (5.36±0.45 mg galantamine equivalent (GALAE)/g), tyrosinase (60.36±0.23 mg kojic acid equivalent (KAE)/g), α-amylase (0.61±0.01 mmol acarbose equivalent (ACAE)/g) and α-glucosidase (0.82±0.01 mmol acarbose equivalent (ACAE)/g) enzymes. Liquid chromatography hyphenated with photodiode-array detection and tandem electrospray ionization mass spectrometry (LC-DAD-MSn) analysis revealed phytochemical fingerprint of the two part of the plant and the most abundant constituents were rutin and orientin for aerial parts, 3,5 and 4,5-dicaffeoyl quinic acids for roots respectively. This is the first report gathering scientific data on antioxidant, enzyme inhibitory activities and phytochemical composition of S. hispanica. Thus, this research can be used as one methodological starting point for further investigation on this plant.
Gunes Ak; Stefano Dall'Acqua; Stefania Sut; Irene Ferrarese; Evren Yıldıztugay; Mohamad Fawzi Mahomoodally; Kouadio Ibrahime Sinan; Nabeelah Bibi Sadeer; Kannan R.R. Rengasamy; Gokhan Zengin. Chemical characterization and bio-pharmaceutical abilities of five different solvent extracts from aerial parts and roots of Scorzonera hispanica L. South African Journal of Botany 2020, 133, 212 -221.
AMA StyleGunes Ak, Stefano Dall'Acqua, Stefania Sut, Irene Ferrarese, Evren Yıldıztugay, Mohamad Fawzi Mahomoodally, Kouadio Ibrahime Sinan, Nabeelah Bibi Sadeer, Kannan R.R. Rengasamy, Gokhan Zengin. Chemical characterization and bio-pharmaceutical abilities of five different solvent extracts from aerial parts and roots of Scorzonera hispanica L. South African Journal of Botany. 2020; 133 ():212-221.
Chicago/Turabian StyleGunes Ak; Stefano Dall'Acqua; Stefania Sut; Irene Ferrarese; Evren Yıldıztugay; Mohamad Fawzi Mahomoodally; Kouadio Ibrahime Sinan; Nabeelah Bibi Sadeer; Kannan R.R. Rengasamy; Gokhan Zengin. 2020. "Chemical characterization and bio-pharmaceutical abilities of five different solvent extracts from aerial parts and roots of Scorzonera hispanica L." South African Journal of Botany 133, no. : 212-221.
Scorzonera tomentosa L. (family Asteraceae), endemic to Turkey is reputed for its medicinal attributes in the folkloric medicine. However, only few studies on the chemical composition and pharmacological properties of this plant have been published so far. In this direction, this study aimed to shed light on the phenolic composition, antioxidant and key enzyme inhibitory properties of S. tomentosa aerial parts and roots extracts prepared using different solvents (dichloromethane, ethyl acetate, hexane, water and methanol). Overall, the methanolic extracts and the infusion of both plant parts were better sources of total polyphenolics with 40.33 and 39.30 mg gallic acid equivalent (GAE)/g, respectively, while for the aerial parts, 22.42 and 21.84 mg GAE/g were measured for methanolic extract and root infusion, respectively. Extracts of the aerial parts showed considerable amount of total flavonoid content (2.96–34.93 mg rutin equivalent (RE)/g) as compared with the root extract (0.40–2.65 mg RE/g), with highest content observed in methanolic extract. For this reason, the methanolic extract of roots and aerial parts were subjected to liquid chromatography coupled to diode array detection and electrospray ionization tandem mass spectrometry (LC-DAD-MSn) analysis. The methanolic and aqueous extracts of both plant parts proved to be most significant radical quenchers (1.05–45.89 mg trolox equivalent (TE)/g in 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay; and 1.82–82.94 mg TE/g in 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay) and reducing agents (39.40–164.06 mg TE/g in cupric reducing antioxidant capacity (CUPRAC) and 15.54–79.78 mg TE/g in ferric reducing antioxidant power (FRAP)). The dichloromethane extracts were significant metal chelators (13.92 and 6.24 mg ethylenediaminetetraacetate equivalent (EDTAE)/g for aerial parts and roots). Extracts of both plant parts showed selective inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Notable anti-tyrosinase activity was observed for all the extracts (aerial part: 16.40 ± 1.30 - 64.83 ± 0.28 and roots: 6.29 ± 0.50–65.46 ± 0.31 mg kojic acid equivalent (KAE)/g), while modest inhibitory activity against α-amylase (0.08−0.61 mmol acarbose equivalent (ACAE)/g) and α-glucosidase (0.09−0.83 mmol ACAE/g) for all the tested extracts. In addition, to provide the first scientific insight on the biological and chemical profile of S. tomentosa, observations from this present study tend to identify this plant as a valuable source of biologically active molecules for possible phytopharmaceutical and cosmeceuticals exploitation.
Stefano Dall’Acqua; Gunes Ak; Stefania Sut; Irene Ferrarese; Gokhan Zengin; Evren Yıldıztugay; Mohamad Fawzi Mahomoodally; Kouadio Ibrahime Sinan; Devina Lobine. Phenolics from Scorzonera tomentosa L.: Exploring the potential use in industrial applications via an integrated approach. Industrial Crops and Products 2020, 154, 112751 .
AMA StyleStefano Dall’Acqua, Gunes Ak, Stefania Sut, Irene Ferrarese, Gokhan Zengin, Evren Yıldıztugay, Mohamad Fawzi Mahomoodally, Kouadio Ibrahime Sinan, Devina Lobine. Phenolics from Scorzonera tomentosa L.: Exploring the potential use in industrial applications via an integrated approach. Industrial Crops and Products. 2020; 154 ():112751.
Chicago/Turabian StyleStefano Dall’Acqua; Gunes Ak; Stefania Sut; Irene Ferrarese; Gokhan Zengin; Evren Yıldıztugay; Mohamad Fawzi Mahomoodally; Kouadio Ibrahime Sinan; Devina Lobine. 2020. "Phenolics from Scorzonera tomentosa L.: Exploring the potential use in industrial applications via an integrated approach." Industrial Crops and Products 154, no. : 112751.
Muscari turcicum, endemic to south Anatolia, Turkey, represents an unexplored crop plant, with potential therapeutic uses related to its phytochemical composition. In this work, the in vitro antioxidant and enzyme inhibitory activity of flower, leaf and bulb extracts, obtained using different extraction methods were evaluated. A comprehensive polyphenolic and sesquiterpene lactones profiling of the different extracts was also undertaken. For this purpose, UHPLC-QTOF mass spectrometry allowed us to putatively annotate 280 phytochemical compounds of which 162 were polyphenols and 118 were sesquiterpene lactones. The most abundant polyphenols were flavonoids (77 compounds), phenolic acids (34 compounds), and low molecular weight phenols (38 compounds). Muscari turcicum leaf methanol extract possessed the highest concentrations of low-molecular-weight phenolics, phenolic acids, and sesquiterpene lactones (20.61, 7.00, and 3.44 mg standard equivalent/g, respectively). The water extract of M. turcicum flower obtained by infusion showed prominent reducing (120.52 mg Trolox equivalent [TE]/g mg TE/g for both CUPRAC and FRAP) and radical scavenging potential (91.39 mg TE/g, for DPPH assay). Besides, M. turcicum flower methanol extract (13.44 mg EDTA equivalent/g) showed the highest metal chelating activity. Interestingly, methanol extracts obtained by Soxhlet extraction and maceration actively inhibited tyrosinase (129.36 mg kojic acid equivalent/g) and cholinesterases (5.15 mg galantamine equivalent [GALAE]/g and 6.16 mg GALAE/g, for acetyl and butyryl cholinesterase) respectively. Strong correlations (p < 0.01) were observed between polyphenols/sesquiterpenoids and observed biological activities. Scientific evidences presented in this study has provided baseline data for bioprospection of novel pharmaceutical/cosmetic candidates from Muscari turcicum, thus supporting its therapeutic exploitation.
Leilei Zhang; Gabriele Rocchetti; Gokhan Zengin; Gunes Ak; Evren Yıldıztugay; Mohamad Fawzi Mahomoodally; Marie Carene Nancy Picot-Allain; Luigi Lucini. Profiling of polyphenols and sesquiterpenoids using different extraction methods in Muscari turcicum, an endemic plant from Turkey. Industrial Crops and Products 2020, 154, 112626 .
AMA StyleLeilei Zhang, Gabriele Rocchetti, Gokhan Zengin, Gunes Ak, Evren Yıldıztugay, Mohamad Fawzi Mahomoodally, Marie Carene Nancy Picot-Allain, Luigi Lucini. Profiling of polyphenols and sesquiterpenoids using different extraction methods in Muscari turcicum, an endemic plant from Turkey. Industrial Crops and Products. 2020; 154 ():112626.
Chicago/Turabian StyleLeilei Zhang; Gabriele Rocchetti; Gokhan Zengin; Gunes Ak; Evren Yıldıztugay; Mohamad Fawzi Mahomoodally; Marie Carene Nancy Picot-Allain; Luigi Lucini. 2020. "Profiling of polyphenols and sesquiterpenoids using different extraction methods in Muscari turcicum, an endemic plant from Turkey." Industrial Crops and Products 154, no. : 112626.
The current study was conducted to demonstrate the possible roles of exogenously applied flavonoid naringenin (Nar) on the efficiency of PSII photochemistry and the responses of chloroplastic antioxidant of salt and osmotic-stressed Phaseolus vulgaris (cv. Yunus90). For this aim, plants were grown in a hydroponic culture and were treated with Nar (0.1 mM and 0.4 mM) alone or in a combination with salt (100 mM NaCl) and/or osmotic (10% Polyethylene glycol, −0.54 MPa). Both caused a reduction in water content (RWC), osmotic potential (ΨΠ), chlorophyll fluorescence (Fv/Fm), and potential photochemical efficiency (Fv/Fo). Nar reversed the changes on these parameters. The phenomenological fluxes (TRo/CS and ETo/CS) altered by stress were induced by Nar and Nar led to a notable increase in the performance index (PIABS) and the capacity of light reaction [ΦPo/(1-ΦPo)]. Besides, Nar-applied plants exhibited higher specific fluxes values [ABS/RC, ETo/RC, and ΨEo/(1-ΨEo)] and decreasing controlled dissipation of energy (DIo/CSo and DIo/RC). The transcripts levels of psbA and psbD were lowered in stress-treated bean but upregulated in Nar-treated plants after stress exposure. Nar also alleviated the changes on gas exchange parameters [carbon assimilation rate (A), stomatal conductance (gs), intercellular CO2 concentrations (Ci), transpiration rate (E), and stomatal limitation (Ls)]. By regulating the antioxidant metabolism of the isolated chloroplasts, Nar was able to control the toxic levels of hydrogen peroxide (H2O2) and TBARS (lipid peroxidation) produced by stresses. Chloroplastic superoxide dismutase (SOD) activity reduced by stresses was increased by Nar. In response to NaCl, Nar increased the activities of ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR), as well as peroxidase (POX). Nar protected the bean chloroplasts by minimizing disturbances caused by NaCl exposure via the ascorbate (AsA) and glutathione (GSH) redox-based systems. Under Nar plus PEG, Nar maintained the AsA regeneration by the induction of MDHAR and DHAR, but not GSH recycling by virtue of no induction in GR activity and the reduction in GSH/GSSG and GSH redox state. Based on these advances, Nar protected in bean chloroplasts by minimizing disturbances caused by NaCl or PEG exposure via the AsA or GSH redox-based systems and POX activity.
Evren Yildiztugay; Ceyda Ozfidan-Konakci; Mustafa Kucukoduk; Ismail Turkan. Flavonoid Naringenin Alleviates Short-Term Osmotic and Salinity Stresses Through Regulating Photosynthetic Machinery and Chloroplastic Antioxidant Metabolism in Phaseolus vulgaris. Frontiers in Plant Science 2020, 11, 682 .
AMA StyleEvren Yildiztugay, Ceyda Ozfidan-Konakci, Mustafa Kucukoduk, Ismail Turkan. Flavonoid Naringenin Alleviates Short-Term Osmotic and Salinity Stresses Through Regulating Photosynthetic Machinery and Chloroplastic Antioxidant Metabolism in Phaseolus vulgaris. Frontiers in Plant Science. 2020; 11 ():682.
Chicago/Turabian StyleEvren Yildiztugay; Ceyda Ozfidan-Konakci; Mustafa Kucukoduk; Ismail Turkan. 2020. "Flavonoid Naringenin Alleviates Short-Term Osmotic and Salinity Stresses Through Regulating Photosynthetic Machinery and Chloroplastic Antioxidant Metabolism in Phaseolus vulgaris." Frontiers in Plant Science 11, no. : 682.
Members of Scorzonera genus are known for their potential application in both food and pharmaceutical industries. The present study reports the chemical component and bioactivity of the dichloromethane, ethyl acetate, hexane, methanol and infused extracts of Scorzonera hieraciifolia Hayek aerial parts and roots. The methanolic extracts of both aerial parts and roots were good sources of total phenolic and flavonoid content. For the aerial parts, the infused and methanolic extracts showed highest (p<0.05) free radical scavenging (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS)), reducing (cupric reducing antioxidant capacity (CUPRAC))and ferric reducing antioxidant power (FRAP)) and metal chelating capacities. Whilst, for the root extracts, overall most remarkable free scavenging and reducing activity was observed for the methanolic extracts (p<0.05) while the hexane extracts showed highest metal chelating activity. The inhibitory activity of the extracts was evaluated against key enzymes. Overall, the hexane extracts of both aerial parts (acetylcholinesterase (AChE): 2.27±0.04 and butyrlcholinesterase (BChE): 5.75±0.48 mg galantamine equivalent (GALAE)/g) and roots (AChE: 2.44±0.35 and BChE: 53.17±0.22 mg GALAE/g) were most potent against AChE and BChE, suggesting a role for the more lipophilic compounds in this regard. The highest inhibitory activity against tyrosinase was displayed by methanolic extracts of aerial parts (64.70±0.26 mg kojic acid equivalent (KAE)/g) and roots (63.63±0.20 mg KAE/g). Modest activity was observed against α-amylase and α-glucosidase, two major enzymes implicated in the pathology of diabetes. Liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) analysis showed the presence of 40 compounds such as caffeic, ursolic, betulinic and oleanolic acid and their derivatives in the studied extracts. Multivariate analysis showed that the biological activities were dependent on the plant parts used as well as on the type of solvent used. Overall, the results showed that S. hieraciifolia is a valuable source of phytochemicals for both the nutraceutical and pharmaceutical sectors.
Stefano Dall'Acqua; Gunes Ak; Stefania Sut; Gokhan Zengin; Evren Yıldıztugay; Mohamad Fawzi Mahomoodally; Kouadio Ibrahime Sinan; Devina Lobine. Comprehensive bioactivity and chemical characterization of the endemic plant Scorzonera hieraciifolia Hayek extracts: A promising source of bioactive compounds. Food Research International 2020, 137, 109371 .
AMA StyleStefano Dall'Acqua, Gunes Ak, Stefania Sut, Gokhan Zengin, Evren Yıldıztugay, Mohamad Fawzi Mahomoodally, Kouadio Ibrahime Sinan, Devina Lobine. Comprehensive bioactivity and chemical characterization of the endemic plant Scorzonera hieraciifolia Hayek extracts: A promising source of bioactive compounds. Food Research International. 2020; 137 ():109371.
Chicago/Turabian StyleStefano Dall'Acqua; Gunes Ak; Stefania Sut; Gokhan Zengin; Evren Yıldıztugay; Mohamad Fawzi Mahomoodally; Kouadio Ibrahime Sinan; Devina Lobine. 2020. "Comprehensive bioactivity and chemical characterization of the endemic plant Scorzonera hieraciifolia Hayek extracts: A promising source of bioactive compounds." Food Research International 137, no. : 109371.
Oryza sativa L. cv. Gönen grown in hydroponic culture was treated with scandium (Sc; 25 and 50 μM) alone or in combination with salt (100 mM NaCl) and/or drought (5% PEG-6000). Stress caused a decrease in growth (RGR), water content (RWC), osmotic potential (ΨΠ), chlorophyll fluorescence (Fv/Fm) and potential photochemical efficiency (Fv/Fo). Sc application prevented the decreases of these parameters. Sc also alleviated the changes on gas exchange parameters (carbon assimilation rate (A), stomatal conductance (gs), intercellular CO2 concentrations (Ci), transpiration rate (E) and stomatal limitation (Ls)). Stress caused no increase in superoxide dismutase (SOD) activity. After induvial applied NaCl or PEG, catalase (CAT) and ascorbate peroxidase (APX) showed an enhancement in activation and tried to scavenge of hydrogen peroxide (H2O2). On the other hand, in plants with the combination form of NaCl and PEG, only CAT activity was induced. Sc applications to NaCl-treated rice led to an increase of SOD, APX, glutathione reductase (GR), monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) as well as peroxidase (POX). Sc under NaCl could be maintained both ascorbate (AsA) and glutathione (GSH) regeneration. Despite of induction of MDHAR and DHAR under Sc plus PEG, Sc did not maintain AsA redox state because of no induction in APX activity. However, GSH pool could be regenerated by induction in DHAR and GR in this group. Sc application (especially for 25 μM) in rice exposed to NaCl + PEG resulted an enhancement in APX and MDHAR and so Sc could be partially provided AsA regeneration. Since no increases in DHAR and GR were observed, GSH pool was reduced. Due to this activation of antioxidant enzymes, stress-induced H2O2 and TBARS content (lipid peroxidation) significantly decreased in rice with Sc applications. Sc in plants with stress also increased the transcript levels of OsCDPK7 and OsBG1 related to stomatal movement and signaling pathway. Consequently, Sc protected the rice plants by minimizing disturbances caused by NaCl or PEG exposure via the AsA-GSH redox-based systems.
Fevzi Elbasan; Ceyda Ozfidan-Konakci; Evren Yildiztugay; Mustafa Kucukoduk. Rare-earth element scandium improves stomatal regulation and enhances salt and drought stress tolerance by up-regulating antioxidant responses of Oryza sativa. Plant Physiology and Biochemistry 2020, 152, 157 -169.
AMA StyleFevzi Elbasan, Ceyda Ozfidan-Konakci, Evren Yildiztugay, Mustafa Kucukoduk. Rare-earth element scandium improves stomatal regulation and enhances salt and drought stress tolerance by up-regulating antioxidant responses of Oryza sativa. Plant Physiology and Biochemistry. 2020; 152 ():157-169.
Chicago/Turabian StyleFevzi Elbasan; Ceyda Ozfidan-Konakci; Evren Yildiztugay; Mustafa Kucukoduk. 2020. "Rare-earth element scandium improves stomatal regulation and enhances salt and drought stress tolerance by up-regulating antioxidant responses of Oryza sativa." Plant Physiology and Biochemistry 152, no. : 157-169.
In this work, the phytochemical profile and the biological properties of Colchicum triphyllum (an unexplored Turkish cultivar belonging to Colchicaceae) have been comprehensively investigated for the first time. Herein, we focused on the evaluation of the in vitro antioxidant and enzyme inhibitory effects of flower, tuber, and leaf extracts, obtained using different extraction methods, namely maceration (both aqueous and methanolic), infusion, and Soxhlet. Besides, the complete phenolic and alkaloid untargeted metabolomic profiling of the different extracts was investigated. In this regard, ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) allowed us to putatively annotate 285 compounds when considering the different matrix extracts, including mainly alkaloids, flavonoids, lignans, phenolic acids, and tyrosol equivalents. The most abundant polyphenols were flavonoids (119 compounds), while colchicine, demecolcine, and lumicolchicine isomers were some of the most widespread alkaloids in each extract analyzed. In addition, our findings showed that C. triphyllum tuber extracts were a superior source of both total alkaloids and total polyphenols, being on average 2.89 and 10.41 mg/g, respectively. Multivariate statistics following metabolomics allowed for the detection of those compounds most affected by the different extraction methods. Overall, C. triphyllum leaf extracts showed a strong in vitro antioxidant capacity, in terms of cupric reducing antioxidant power (CUPRAC; on average 96.45 mg Trolox Equivalents (TE)/g) and ferric reducing antioxidant power (FRAP) reducing power (on average 66.86 mg TE/g). Interestingly, each C. triphyllum methanolic extract analyzed (i.e., from tuber, leaf, and flower) was active against the tyrosinase in terms of inhibition, recording the higher values for methanolic macerated leaves (i.e., 125.78 mg kojic acid equivalent (KAE)/g). On the other hand, moderate inhibitory activities were observed against AChE and α-amylase. Strong correlations (p < 0.01) were also observed between the phytochemical profiles and the biological activities determined. Therefore, our findings highlighted, for the first time, the potential of C. triphhyllum extracts in food and pharmaceutical applications.
Biancamaria Senizza; Gabriele Rocchetti; Murat Ali Okur; Gokhan Zengin; Evren Yıldıztugay; Gunes Ak; Domenico Montesano; Luigi Lucini. Phytochemical Profile and Biological Properties of Colchicum triphyllum (Meadow Saffron). Foods 2020, 9, 457 .
AMA StyleBiancamaria Senizza, Gabriele Rocchetti, Murat Ali Okur, Gokhan Zengin, Evren Yıldıztugay, Gunes Ak, Domenico Montesano, Luigi Lucini. Phytochemical Profile and Biological Properties of Colchicum triphyllum (Meadow Saffron). Foods. 2020; 9 (4):457.
Chicago/Turabian StyleBiancamaria Senizza; Gabriele Rocchetti; Murat Ali Okur; Gokhan Zengin; Evren Yıldıztugay; Gunes Ak; Domenico Montesano; Luigi Lucini. 2020. "Phytochemical Profile and Biological Properties of Colchicum triphyllum (Meadow Saffron)." Foods 9, no. 4: 457.
The role of hydrogen sulfide (H2S)/nitric oxide (NO) in mitigating stress-induced damages has gained interest in the past few years. However, the protective mechanism H2S and/or NO has towards the chloroplast system through the regulation of redox status and activation of antioxidant capacity in cobalt-treated wheat remain largely unanswered. Triticum aestivum L. cv. Ekiz was treated with alone/in combination of a H2S donor (sodium hydrosulfide (NaHS,600μM)), a NO donor (sodium nitroprusside (SNP,100μM)) and a NO scavenger (rutin hydrate (RTN,50μM)) to assess how the donors affect growth, water relations, redox and antioxidant capacity in chloroplasts, under cobalt (Co) concentrations of 150-300 μM. Stress decreased a number of parameters (growth, water content (RWC), osmotic potential (ΨΠ), carbon assimilation rate, stomatal conductance, intercellular CO2 concentrations, transpiration rate and the transcript levels of rubisco, which subsequently disrupt the photosynthetic capacity). However, SNP/NaHS counteracted the negative effects of stress on these aforementioned parameters and RTN application with stress/non-stress was reversed these effects. Hydrogen peroxide (H2O2) and TBARS were induced under stress in spite of activated ascorbate peroxidase (APX). SNP/NaHS under stress increased activation of superoxide dismutase (SOD), peroxidase (POX), APX, glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), ascorbate (tAsA) and glutathione (GSH). In conclusion, NaHS/SNP are involved in the regulation and modification of growth, water content, rubisco activity and up-regulation of ascorbate-glutathione cycle (AsA-GSH) in chloroplast under stress.
Ceyda Ozfidan-Konakci; Evren Yildiztugay; Fevzi Elbasan; Mustafa Kucukoduk; Ismail Turkan. Hydrogen sulfide (H2S) and nitric oxide (NO) alleviate cobalt toxicity in wheat (Triticum aestivum L.) by modulating photosynthesis, chloroplastic redox and antioxidant capacity. Journal of Hazardous Materials 2020, 388, 122061 .
AMA StyleCeyda Ozfidan-Konakci, Evren Yildiztugay, Fevzi Elbasan, Mustafa Kucukoduk, Ismail Turkan. Hydrogen sulfide (H2S) and nitric oxide (NO) alleviate cobalt toxicity in wheat (Triticum aestivum L.) by modulating photosynthesis, chloroplastic redox and antioxidant capacity. Journal of Hazardous Materials. 2020; 388 ():122061.
Chicago/Turabian StyleCeyda Ozfidan-Konakci; Evren Yildiztugay; Fevzi Elbasan; Mustafa Kucukoduk; Ismail Turkan. 2020. "Hydrogen sulfide (H2S) and nitric oxide (NO) alleviate cobalt toxicity in wheat (Triticum aestivum L.) by modulating photosynthesis, chloroplastic redox and antioxidant capacity." Journal of Hazardous Materials 388, no. : 122061.
Ceyda Özfidan-Konakçi; Evren Yildiztugay; Fevzi Elbasan; Ayşegül Yildiztugay; Mustafa Küçüködük. Assessment of antioxidant system and enzyme/nonenzyme regulation related to ascorbate-glutathione cycle in ferulic acid-treated Triticumaestivum L. roots under boron toxicity. TURKISH JOURNAL OF BOTANY 2020, 44, 47 -61.
AMA StyleCeyda Özfidan-Konakçi, Evren Yildiztugay, Fevzi Elbasan, Ayşegül Yildiztugay, Mustafa Küçüködük. Assessment of antioxidant system and enzyme/nonenzyme regulation related to ascorbate-glutathione cycle in ferulic acid-treated Triticumaestivum L. roots under boron toxicity. TURKISH JOURNAL OF BOTANY. 2020; 44 (1):47-61.
Chicago/Turabian StyleCeyda Özfidan-Konakçi; Evren Yildiztugay; Fevzi Elbasan; Ayşegül Yildiztugay; Mustafa Küçüködük. 2020. "Assessment of antioxidant system and enzyme/nonenzyme regulation related to ascorbate-glutathione cycle in ferulic acid-treated Triticumaestivum L. roots under boron toxicity." TURKISH JOURNAL OF BOTANY 44, no. 1: 47-61.
Like other members of the Colchicum genus, C. szovitsii subsp. szovitsii is also of medicinal importance in Turkish traditional medicine. However, its biological properties have not been fully investigated. Herein, we focused on the evaluation of the in vitro antioxidant and enzyme inhibitory effects of flower, root and leaf extracts, obtained using different extraction methods. In addition, a comprehensive (poly)-phenolic and alkaloid profiling of the different extracts was undertaken. In this regard, ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) allowed us to putatively annotate 195 polyphenols and 87 alkaloids. The most abundant polyphenols were flavonoids (83 compounds), whilst colchicine and 2-demethylcolchicine were some of the most widespread alkaloids in each extract analyzed. However, our findings showed that C. szovitsii leaf extracts were a superior source of both total polyphenols and total alkaloids (being, on average 24.00 and 2.50 mg/g, respectively). Overall, methanolic leaf extracts showed the highest (p < 0.05) ferric reducing antioxidant power (FRAP) reducing power (on average 109.52 mgTE/g) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging (on average 90.98 mgTE/g). Interestingly, each C. szovitsii methanolic extract was more active than the water extracts when considering enzymatic inhibition such as against tyrosinase, glucosidase, and acetylcholinesterase (AChE). Strong correlations (p < 0.01) were also observed between polyphenols/alkaloids and the biological activities determined. Multivariate statistics based on supervised orthogonal projections to latent structures discriminant analysis (OPLS-DA) allowed for the detection of those compounds most affected by the different extraction methods. Therefore, this is the first detailed evidence showing that C. szovitsii subsp. szovitsii might provide beneficial effects against oxidative stress and the associated chronic diseases. Nevertheless, the detailed mechanisms of action need to be further investigated.
Gabriele Rocchetti; Biancamaria Senizza; Gokhan Zengin; Murat Ali Okur; Domenico Montesano; Evren Yildiztugay; Devina Lobine; Mohamad Fawzi Mahomoodally; Luigi Lucini. Chemical Profiling and Biological Properties of Extracts from Different Parts of Colchicum Szovitsii Subsp. Szovitsii. Antioxidants 2019, 8, 632 .
AMA StyleGabriele Rocchetti, Biancamaria Senizza, Gokhan Zengin, Murat Ali Okur, Domenico Montesano, Evren Yildiztugay, Devina Lobine, Mohamad Fawzi Mahomoodally, Luigi Lucini. Chemical Profiling and Biological Properties of Extracts from Different Parts of Colchicum Szovitsii Subsp. Szovitsii. Antioxidants. 2019; 8 (12):632.
Chicago/Turabian StyleGabriele Rocchetti; Biancamaria Senizza; Gokhan Zengin; Murat Ali Okur; Domenico Montesano; Evren Yildiztugay; Devina Lobine; Mohamad Fawzi Mahomoodally; Luigi Lucini. 2019. "Chemical Profiling and Biological Properties of Extracts from Different Parts of Colchicum Szovitsii Subsp. Szovitsii." Antioxidants 8, no. 12: 632.
To eliminate the damages of metal toxicity by reducing metal uptake by plants, organic amendments are useful. The use of carbon-rich materials known as biochar (BC) is a strong candidate to enhance the plant tolerance against stress conditions. The current study examined the effects of BC in wheat hydroponically grown treated with BC (1 and 3 g L−1) alone or in combination with cobalt (Co, 150 and 300 μM). Stress reduced the relative growth rate (RGR), relative water content (RWC), osmotic potential (ΨΠ), and increased proline content (Pro). Besides, endogenous contents of Ca2+, K+, and Mn2+ in leaves decreased under stress. In response to Co stress, a decline in the activities of peroxidase (POX), ascorbate peroxidase (APX), and glutathione reductase (GR) resulted in the induction of hydrogen peroxide (H2O2) content. BC applied with stress decreased endogenous Co2+ content and increased RGR, RWC, chlorophyll fluorescence and Pro content. Also, the activities of superoxide dismutase (SOD), catalase (CAT), APX and GR were induced and the ascorbate (AsA) and glutathione (GSH) pool and their redox state were maintained by BC application under stress condition. While, with the addition of BC, H2O2 content and lipid peroxidation displayed remarkable decreased, the scavenging activity of hydroxyl radical (OH·) increased as compared to Co stress-treated wheat plants. Besides, in wheat leaves, BC application triggered AsA-GSH pathway including activities of monodehydroascorbate reductase, dehydroascorbate reductase, and the contents of dehydroascorbate, GSH, and GSH/GSSG ratio. The presented results supported the view that biochar under stress could minimize the Co-induced oxidative damages through modulation of the growth, water status, photosynthetic apparatus, and antioxidant enzyme activity found in cellular compartments and ascorbate-glutathione cycle in wheat leaves.
Aysegul Yildiztugay; Ceyda Ozfidan-Konakci; Evren Yildiztugay; Mustafa Kucukoduk. Biochar Triggers Systemic Tolerance Against Cobalt Stress in Wheat Leaves Through Regulation of Water Status and Antioxidant Metabolism. Journal of Soil Science and Plant Nutrition 2019, 19, 935 -947.
AMA StyleAysegul Yildiztugay, Ceyda Ozfidan-Konakci, Evren Yildiztugay, Mustafa Kucukoduk. Biochar Triggers Systemic Tolerance Against Cobalt Stress in Wheat Leaves Through Regulation of Water Status and Antioxidant Metabolism. Journal of Soil Science and Plant Nutrition. 2019; 19 (4):935-947.
Chicago/Turabian StyleAysegul Yildiztugay; Ceyda Ozfidan-Konakci; Evren Yildiztugay; Mustafa Kucukoduk. 2019. "Biochar Triggers Systemic Tolerance Against Cobalt Stress in Wheat Leaves Through Regulation of Water Status and Antioxidant Metabolism." Journal of Soil Science and Plant Nutrition 19, no. 4: 935-947.
Ethnobotanical evidences substantiate the use of several Centaurea species to treat and/or manage several human ailments. In the present study, the phytochemical profile of the ethyl acetate, methanol, and aqueous extracts (prepared by infusion and decoction) of Centaurea bornmuelleri Hausskn. aerial parts was established. The enzyme inhibitory and antioxidant properties were also determined by in vitro bioassays. Methanol extract (38.58 mg gallic acid equivalent/g extract) and ethyl acetate extract (38.83 mg rutin equivalent/g extract) possessed the highest concentration of phenolics and flavonoids, respectively. Aqueous extract prepared following traditional infusion method showed potent DPPH (38.54 mg TE/g extract) and ABTS (57.75 mg TE/g extract) scavenging abilities. The methanol extract (101.46 mg TE/g extract) of C. bornmuelleri exhibited potent reducing activity in the CUPRAC assay while the aqueous extract obtained by infusion was more active in the FRAP assay (69.81 mg TE/g extract). Ethyl acetate extract of C. bornmuelleri inhibited both acetylcholinesterase (1.14 mg galantamine equivalent [GALAE]/g extract), butyrylcholinesterase (0.63 mg GALAE/g extract), tyrosinase (69.84 mg kojic acid equivalent/g extract), amylase (19.90 mg acarbose equivalent [ACAE]/g extract), and glucosidase (33.12 mg ACAE/g extract). The phytochemical profile of C. bornmuelleri has been characterized and the main components quantified in order to provide scientific base to design innovative products including pharmaceuticals, cosmetics or nutraceuticals although further investigation concerning the isolation of the main bioactive compounds would be required.
Gokhan Zengin; E.J. Llorent-Martínez; Kouadio Ibrahime Sinan; Evren Yıldıztugay; Carene Picot-Allain; Mohamad Fawzi Mahomoodally. Chemical profiling of Centaurea bornmuelleri Hausskn. aerial parts by HPLC-MS/MS and their pharmaceutical effects: From nature to novel perspectives. Journal of Pharmaceutical and Biomedical Analysis 2019, 174, 406 -413.
AMA StyleGokhan Zengin, E.J. Llorent-Martínez, Kouadio Ibrahime Sinan, Evren Yıldıztugay, Carene Picot-Allain, Mohamad Fawzi Mahomoodally. Chemical profiling of Centaurea bornmuelleri Hausskn. aerial parts by HPLC-MS/MS and their pharmaceutical effects: From nature to novel perspectives. Journal of Pharmaceutical and Biomedical Analysis. 2019; 174 ():406-413.
Chicago/Turabian StyleGokhan Zengin; E.J. Llorent-Martínez; Kouadio Ibrahime Sinan; Evren Yıldıztugay; Carene Picot-Allain; Mohamad Fawzi Mahomoodally. 2019. "Chemical profiling of Centaurea bornmuelleri Hausskn. aerial parts by HPLC-MS/MS and their pharmaceutical effects: From nature to novel perspectives." Journal of Pharmaceutical and Biomedical Analysis 174, no. : 406-413.
Evren Yildiztugay; Ceyda Ozfidan-Konakci; Huseyin Karahan; Mustafa Kucukoduk; Ismail Turkan. Ferulic acid confers tolerance against excess boron by regulating ROS levels and inducing antioxidant system in wheat leaves (Triticum aestivum). Environmental and Experimental Botany 2019, 161, 193 -202.
AMA StyleEvren Yildiztugay, Ceyda Ozfidan-Konakci, Huseyin Karahan, Mustafa Kucukoduk, Ismail Turkan. Ferulic acid confers tolerance against excess boron by regulating ROS levels and inducing antioxidant system in wheat leaves (Triticum aestivum). Environmental and Experimental Botany. 2019; 161 ():193-202.
Chicago/Turabian StyleEvren Yildiztugay; Ceyda Ozfidan-Konakci; Huseyin Karahan; Mustafa Kucukoduk; Ismail Turkan. 2019. "Ferulic acid confers tolerance against excess boron by regulating ROS levels and inducing antioxidant system in wheat leaves (Triticum aestivum)." Environmental and Experimental Botany 161, no. : 193-202.
Gallic acid (GLA; 3,4,5- trihydroxybenzoic acid) is a strong antioxidant in plants. In order to clarify the effects of GLA as a pro-oxidant or an antioxidant on cells under stress conditions, soybean (Glycine max) was grown under normal conditions or in the presence of cold stress (5 and 10?C) in the absence or presence of gallic acid (GLA; 1 and 2 mM) for 72 h. The soybean roots exposed to stress exhibited a significant decline in growth (RGR), water content (RWC), osmotic potential (??) and proline content (Pro). However, GLA treatment under stress significantly improved these parameters and alleviated the stress-generated damage. Stress decreased superoxide dismutase (SOD) activity, but GLA effectively mitigated the adverse effects on enzyme activity. After stress treatment, only catalase (CAT) was induced in soybean roots, although it was not sufficient to prevent toxic hydrogen peroxide (H2O2) accumulation. Thus, the levels of lipid peroxidation (TBARS content) markedly increased. However, GLA contributed to detoxification of H2O2 and lipid peroxidation by enhancing activities of CAT and peroxidase (POX). In addition to these enzymes, SOD activity was able to scavenge superoxide anion radicals, as evidenced by decline in TBARS content. However, monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), total ascorbate (tAsA) and glutathione (GSH) showed a decline of content in roots treated with GLA (both concentrations) plus stress. Our results suggest a protective role of GLA, which may strengthen plant tolerance by ensuring efficient water use and enhancing antioxidant systems. In soybean roots, GLA successfully alleviated the toxicity of cold stress by modulating the activities of SOD, CAT and POX rather than enzymes of the ascorbate-glutathione cycle.
Ceyda Ozfidan-Konakci; Evren Yildiztugay; Aysegul Yildiztugay; Mustafa Kucukoduk. Cold stress in soybean (Glycine max L.) roots: Exogenous gallic acid promotes water status and increases antioxidant activities. Botanica Serbica 2019, 43, 59 -71.
AMA StyleCeyda Ozfidan-Konakci, Evren Yildiztugay, Aysegul Yildiztugay, Mustafa Kucukoduk. Cold stress in soybean (Glycine max L.) roots: Exogenous gallic acid promotes water status and increases antioxidant activities. Botanica Serbica. 2019; 43 (1):59-71.
Chicago/Turabian StyleCeyda Ozfidan-Konakci; Evren Yildiztugay; Aysegul Yildiztugay; Mustafa Kucukoduk. 2019. "Cold stress in soybean (Glycine max L.) roots: Exogenous gallic acid promotes water status and increases antioxidant activities." Botanica Serbica 43, no. 1: 59-71.
Species of the Crocus and Cyclamen genus have been reported to possess diverse biological properties. In the current work, the antioxidant and cytotoxic effects of the methanolic extracts of C. pallasii and C. cilicium aerial and underground parts were evaluated. The flower extracts of C. pallasii and C. cilicium possessed highest flavonoid content. Highest phenolic content was recorded from C. cilicium root extract (47.62 mg gallic acid equivalent/g extract). C. cilicium root extract showed significantly (p < 0.05) high radical scavenging (94.28 and 139.60 mg trolox equivalent [TE]/g extract, against DPPH and ABTS radicals, respectively) and reducing potential (173.30 and 109.53 mg TE/g extract, against CUPRAC and FRAP, respectively). Methanolic extracts of C. pallasii and C. cilicium showed toxicity against breast cancer cell lines. In the light of the above findings, C. cilicium might be considered as an interesting candidate in the development of anti-cancer agent, possessing antioxidant properties.
Gokhan Zengin; Evren Yildiztugay. Focusing on the Chemical Characterization, Antioxidant and Cytotoxic Properties of Two Geophytes: Crocus Pallasii and Cyclamen Cilicium. Proceedings 2019, 40, 17 .
AMA StyleGokhan Zengin, Evren Yildiztugay. Focusing on the Chemical Characterization, Antioxidant and Cytotoxic Properties of Two Geophytes: Crocus Pallasii and Cyclamen Cilicium. Proceedings. 2019; 40 (1):17.
Chicago/Turabian StyleGokhan Zengin; Evren Yildiztugay. 2019. "Focusing on the Chemical Characterization, Antioxidant and Cytotoxic Properties of Two Geophytes: Crocus Pallasii and Cyclamen Cilicium." Proceedings 40, no. 1: 17.