The opacity of conventional ultrasound transducers can impede the miniaturization and workflow of current photoacoustic systems. In particular, optical-resolution photoacoustic microscopy (OR-PAM) requires the coaxial alignment of optical illumination and acoustic-detection paths through complex beam combiners and a thick coupling medium. To overcome these hurdles, we developed a novel OR-PAM method on the basis of our recently reported transparent lithium niobate (LiNbO₃) ultrasound transducer (Dangi et al., Optics Letters, 2019), which was centered at 13 MHz ultrasound frequency with 60% photoacoustic bandwidth. To test the feasibility of wearable OR-PAM, optical-only raster scanning of focused light through a transducer was performed while the transducer was fixed above the imaging subject. Imaging experiments on resolution targets and carbon fibers demonstrated a lateral resolution of 8.5 µm. Further, we demonstrated vasculature mapping using chicken embryos and melanoma depth profiling using tissue phantoms. In conclusion, the proposed OR-PAM system using a low-cost transparent LiNbO₃ window transducer has a promising future in wearable and high-throughput imaging applications, e.g., integration with conventional optical microscopy to enable a multimodal microscopy platform capable of ultrasound stimulation.

We recently introduced piezoelectric lithium niobate (LN) based transparent ultrasound transducers (TUT) as a new platform for developing multimodal optical, ultrasound, and photoacoustic imaging (PAI) systems. However, LN based TUT is limited in its signal-to-noise ratio due to material's low piezoelectricity ( $d_{33}$ ). In this letter, we report, for the first time, a 0.2-mm-thick transparent lead magnesium niobate-lead titanate (PMN-PT) based TUT (PMN-PT-TUT) for ultrasound and photoacoustic applications and compared its performance with a 0.25-mm-thick transparent LN based TUT (LN-TUT). To improve the ultrasound energy transmission efficiency, TUTs were fabricated with a two-matching-layer design. This resulted in a dual frequency response with center frequencies of 7.8 MHz/13.2 MHz and corresponding bandwidths of 28.2%/66.67% for PMN-PT-TUT, as well as center frequencies of 7.2 MHz/11.8 MHz and bandwidths of 36.1%/62.7% for LN-TUT. The optical transmission rate of PMN-PT-TUTs and LN-TUTs are measured as $\sim$ 73 and $\sim$ 91%, respectively, at 532 nm optical wavelength. The PMN-PT-TUT exhibited higher sensitivity compared to LN-TUT with a nearly threefold higher pulse echo amplitude and more than twofold higher photoacoustic amplitude. Furthermore, optical resolution photoacoustic microscopy experiments on phantom targets demonstrated lateral resolutions of 7 and 5.1 $\mu$ m and axial resolutions of 285.6 and 375.9 $\mu$ m for PMN-PT-TUT and LN-TUT, respectively. These results indicated that PMN-PT is a viable alternative to LN for developing TUT based multimodal ultrasound and PAI systems.

Objective and Impact Statement. Simultaneous imaging of ultrasound and optical contrasts can help map structural, functional, and molecular biomarkers inside living subjects with high spatial resolution. There is a need to develop a platform to facilitate this multimodal imaging capability to improve diagnostic sensitivity and specificity. Introduction . Currently, combining ultrasound, photoacoustic, and optical imaging modalities is challenging because conventional ultrasound transducer arrays are optically opaque. As a result, complex geometries are used to coalign both optical and ultrasound waves in the same field of view. Methods . One elegant solution is to make the ultrasound transducer transparent to light. Here, we demonstrate a novel transparent ultrasound transducer (TUT) linear array fabricated using a transparent lithium niobate piezoelectric material for real-time multimodal imaging. Results . The TUT-array consists of 64 elements and centered at ~6 MHz frequency. We demonstrate a quad-mode ultrasound, Doppler ultrasound, photoacoustic, and fluorescence imaging in real-time using the TUT-array directly coupled to the tissue mimicking phantoms. Conclusion . The TUT-array successfully showed a multimodal imaging capability and has potential applications in diagnosing cancer, neurological, and vascular diseases, including image-guided endoscopy and wearable imaging.

Studying brain‐wide hemodynamic responses to different stimuli at high spatiotemporal resolutions can help gain new insights into the mechanisms of neuro‐ diseases and ‐disorders. Nonetheless, this task is challenging, primarily due to the complexity of neurovascular coupling, which encompasses interdependent hemodynamic parameters including cerebral blood volume (CBV), cerebral blood flow (CBF), and cerebral oxygen saturation (SO₂). The current brain imaging technologies exhibit inherent limitations in resolution, sensitivity, and imaging depth, restricting their capacity to comprehensively capture the intricacies of cerebral functions. To address this, a multimodal functional ultrasound and photoacoustic (fUSPA) imaging platform is reported, which integrates ultrafast ultrasound and multispectral photoacoustic imaging methods in a compact head‐mountable device, to quantitatively map individual dynamics of CBV, CBF, and SO₂ as well as contrast agent enhanced brain imaging at high spatiotemporal resolutions. Following systematic characterization, the fUSPA system is applied to study brain‐wide cerebrovascular reactivity (CVR) at single‐vessel resolution via relative changes in CBV, CBF, and SO₂ in response to hypercapnia stimulation. These results show that cortical veins and arteries exhibit differences in CVR in the stimulated state and consistent anti‐correlation in CBV oscillations during the resting state, demonstrating the multiparametric fUSPA system's unique capabilities in investigating complex mechanisms of brain functions.

Transparent ultrasound transducer (TUT) technology allows easy co-alignment of optical and acoustic beams in the development of compact photoacoustic imaging (PAI) devices with minimum acoustic coupling. However, TUTs suffer from narrow bandwidth and low pulse-echo sensitivity due to the lack of suitable transparent acoustic matching and backing layers. Here, we studied translucent glass beads (GB) in transparent epoxy as an acoustic matching layer for the transparent lithium niobate piezoelectric material-based TUTs (LN-TUTs). The acoustic and optical properties of various volume fractions of GB matching layers were studied using theoretical calculations, simulations, and experiments. These results demonstrated that the GB matching layer has significantly enhanced the pulse-echo sensitivity and bandwidth of the TUTs. Moreover, the GB matching layer served as a light diffuser to help achieve uniform optical fluence on the tissue surface and also improved the photoacoustic (PA) signal bandwidth. The proposed GB matching layer fabrication is low cost, easy to manufacture using conventional ultrasound transducer fabrication tools, acoustically compatible with soft tissue, and minimizes the use of the acoustic coupling medium.

The receive sensitivity of lead zirconate titanate (PZT) piezoelectric micromachined ultrasound transducers (PMUTs) was improved by applying a DC bias during operation. The PMUT receive sensitivity is governed by the voltage piezoelectric coefficient, h_31,f. With applied DC biases (up to 15 V) on a 2 μm PbZr_0.52Ti_0.48O₃ film, e_31,f increased 1.6 times, permittivity decreased by a factor of 0.6, and the voltage coefficient increased by ~2.5 times. For released PMUT devices, the ultrasound receive sensitivity improved by 2.5 times and the photoacoustic signal improved 1.9 times with 15 V applied DC bias. B-mode photoacoustic imaging experiments showed that with DC bias, the PMUT received clearer photoacoustic signals from pencil leads at 4.3 cm, compared to 3.7 cm without DC bias.