Leona Puljić

Prof. Dr. Leona Puljić


Research Focus & Metrics

Academic Career Length

8Years (2019-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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17 December 2019 in Foods
Verified
Journal Article
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LPLeona Puljić
Leona Puljić
Krešimir MastanjevićKrešimir Mastanjević
Krešimir Mastanjević
BKBrankica Kartalovic
Brankica Kartalovic
DKDragan Kovačević
Dragan Kovačević
JVJelena Vranešević
Jelena Vranešević
Kristina HabschiedKristina Habschied
Kristina Habschied
During smoking, meat products may get contaminated by polycyclic aromatic hydrocarbons (PAH), especially the ones that are smoked in traditional (uncontrolled) conditions. This study aims to evaluate the difference in PAH content in samples of traditional dry cured pork meat products, “Hercegovačka pečenica”, produced in (1) a traditional smokehouse and (2) in industrial chambers. The study revealed that the content of the four priority PAHs (PAH4) in samples produced in a traditional smoking manner highly exceeded (up to 10 times) the maximal limits set for PAHs (12 µg/kg). PAH4 in all samples subjected to industrial smoking procedures was below the limit of quantification. All samples had below-the-limit-of-quantification values for Benzo[a]pyrene. The surface layer of the samples produced in traditional conditions had the highest total content of PAH16. The inner parts of all samples, whether traditional or industrial, had significantly lower PAH16 concentration than the surface layer.

17 January 2020 in Foods
Verified
Journal Article
Open access
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BKBrankica Kartalovic
Brankica Kartalovic
Krešimir MastanjevićKrešimir Mastanjević
Krešimir Mastanjević
NNNikolina J Novakov
Nikolina J Novakov
JVJelena Vranešević
Jelena Vranešević
Dragana Branko Ljubojević PelićDragana Branko Ljubojević Pelić
Dragana Branko Ljubojević Pelić
Kristina HabschiedKristina Habschied
Kristina Habschied
The aim of this study was to determine the concentration of 19 organochlorine pesticides (OCPs): (hexachlorocyclohexane (α-HCH, β-HCH, δ–HCH), lindane, aldrin, heptachlor, heptachlor epoxide, trans-chlordane, cis-chlordane, endosulfane I, endosulfane II, endosulfane sulfate, dichlorodiphenyltrichloroethane (DDT), dichlorodiphenyldichloroethylene (DDE), dieldrin, endrin, dichlorodiphenyldichloroethane (DDD), methoxychlor and endrin ketone and 6 polychlorinated biphenyls (PCBs) (PCB 28, PCB 52, PCB 101, PCB 153, PCB 138 and PCB 180). The samples were taken from pancetta, dry pork neck (budiola), pork tenderloin and sausages produced in Rakitno (Bosnia and Herzegovina), smoked in both a traditional smokehouse and in an industrial chamber. Instrumental analysis was performed using gas chromatography–mass spectrometry (GC–MS). The reliability of the results, i.e., quality control is ensured by standard laboratory practice, which involves participation in proficiency test, the use of blank samples, reference materials and implementation of recommendations given by the relevant international organizations. The concentrations of α-HCH, lindane, PCB 28, PCB 52 and PCB 153 were detected and quantified. The concentrations of OCPs and PCBs did not significantly vary depending on product type and the conditions of production. All the examined samples were for human consumption.

2 August 2020 in Processes
Verified
Journal Article
Open access
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Krešimir MastanjevićKrešimir Mastanjević
Krešimir Mastanjević
BKBrankica Kartalovic
Brankica Kartalovic
LPLeona Puljić
Leona Puljić
DKDragan Kovačević
Dragan Kovačević
Kristina HabschiedKristina Habschied
Kristina Habschied
The concentrations of 16 polycyclic aromatic hydrocarbons (PAH) in smoked dry sausage Hercegovačka kobasica were investigated. The sausages were stuffed in two different casings (collagen and natural) and smoked in traditional and industrial smokehouses. The highest concentration of PAH 16 were detected in sausages in natural casings smoked in the traditional manner. The samples smoked in the industrial chamber stuffed in collagen casing showed the lowest PAH 16 content. The content of PAH 4 in sausage smoked in the traditional way and stuffed in natural casing averaged 24.46 µg/kg, which is more the double of maximum prescribed concentration of 12 µg/kg. The concentration of cancerogenic benzo[a]pyrene averaged 7.79 µg/kg in sausage stuffed in natural casing and smoked in the traditional way, which is almost four times the legislative prescribed values (2 µg/kg). Sausage smoked in the traditional manner and stuffed in collagen casing showed lower values for PAH 4 (13.88 µg/kg) and benzo[a]pyrene (4.97 µg/kg), but these values were also above the legislative prescribed values.

3 January 2025 in Processes
Verified
Journal Article
Open access
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LPLeona Puljić
Leona Puljić
MBMarija Banožić
Marija Banožić
NKNikolina Kajić
Nikolina Kajić
VVVišnja Vasilj
Višnja Vasilj
Kristina HabschiedKristina Habschied
Kristina Habschied
Krešimir MastanjevićKrešimir Mastanjević
Krešimir Mastanjević
To ensure food security amid dwindling natural resources, alternative proteins (APs) have been suggested as a sustainable solution. Yet, the adoption and consumption of APs remain limited. This review aims to delve into the latest progress (following PRISMA guidelines) concerning the utilization of proteins from alternative sources, particularly focusing on their effective incorporation into food products. Our findings reveal that insect proteins can improve amino acid profiles in bakery products. However, consumer acceptance remains low due to cultural biases, with optimal sensory results being achieved at lower substitution levels (5–10%). Mushroom proteins, when incorporated into meat analogs and bakery items, enhance nutritional value and offer favorable sensory properties, making them viable replacements in meat products. Plant-based proteins, such as pea and soy proteins, increase fiber and antioxidants and improve texture in meat alternatives, although formulation adjustments are necessary to meet consumer expectations for taste and overall experience. Microalgae offer unique benefits for bakery, confectionery, and dairy products by boosting protein, fatty acids, and probiotic growth while maintaining sensory acceptability. In conclusion, this study highlights that the effective incorporation of APs into food products can help in the development of healthier, more sustainable diets. That said, the success of AP acceptance will depend on continued innovations in formulation and consumer education.

Krešimir MastanjevićKrešimir Mastanjević
Krešimir Mastanjević
LPLeona Puljić
Leona Puljić
BKBrankica Kartalovic
Brankica Kartalovic
JGJozo Grbavac
Jozo Grbavac
MJMarija Jukić-Grbavac
Marija Jukić-Grbavac
Kristina HabschiedKristina Habschied
Kristina Habschied
Hercegovački pršut as a traditional dry-cured smoked ham (prosciutto) produced by using an open fire that can be potentially contaminated with polycyclic aromatic hydrocarbons (PAHs) and can pose a health risk for consumers. The aim of this research was to identify the types and concentrations of 16 PAHs in 34 samples of traditionally smoked prosciutto. Out of 16 investigated PAHs, identified in the EPA (Environmental Protection Agency) list of priority pollutants, 14 compounds were detected. Average levels of cancerogenic benzo[a]pyrene (BaP) and PAH4 (benzo[a]anthracene (BaA), benzo[b]fluoranthene (BbF), chrysene (Chry), and benzo[a]pyrene (BaP)) ranged from <LOQ (level of quantification) to 5.08 μg/kg and 0.45 μg/kg to 22.67 μg/kg. Two analyzed samples exceeded currently prescribed values according to the Bosnia and Herzegovina legislation for BaP concentrations and one sample for PAH4 content. PAH16 concentrations were on average between 2.92 μg/kg and 87.6 μg/kg. The highest PAH concentrations were found in samples from the Herzegovina-Neretva canton. The results of the research highlight the importance of standardizing smoking procedures and manufacturing practice, in the production of Hercegovački pršut, in order to reduce the PAH content.

20 December 2023 in Fermentation
Verified
Journal Article
Open access
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MAMartina Arapović
Martina Arapović
LPLeona Puljić
Leona Puljić
NKNikolina Kajić
Nikolina Kajić
MBMarija Banožić
Marija Banožić
BKBrankica Kartalovic
Brankica Kartalovic
Krešimir MastanjevićKrešimir Mastanjević
Krešimir Mastanjević
The increasing focus on a healthy lifestyle has emphasized a connection between gut microbiome and health. Consumers face the choice between consuming traditional dairy kefirs or more trendy fruit-based fermented drinks. Also, the aim of this study is to determine the similarities and differences theoretically and experimentally based on a higher ratio of grains to make a lower pH-based beverage at different inoculation times and durations. This study also aims to assess acceptability from a sensory perspective. The goal of the research was to make kefir grain-based beverages with a higher number of lactic acid bacteria (LAB) owing to their probiotic impact on the human gut. After analysis, it was found that there was the highest content of LAB in water kefir after 48 h of inoculation. However, consumer acceptance tests found traditional dairy kefir with a 24-h inoculation to be more acceptable. Although both drinks were inoculated in a 1:4 ratio, it is important to note the variations in grain origin as well as the initial composition of the milk–water suspension, which significantly affects the final product. Since the recipe of the kefir determines the benefits of the drink, the onus is on the consumers to decide which fermented drink suits their health condition best.