China has the largest output of litchi in the world. However, at present, litchi is mainly picked manually, fruit farmers have high labor intensity and low efficiency. This means the intelligent unmanned picking system has broad prospects. The precise location of the main stem picking point of litchi is very important for the path planning of an unmanned system. Some researchers have identified the fruit and branches of litchi; however, there is relatively little research on the location of the main stem picking point of litchi. So, this paper presents a new open-access workflow for detecting accurate picking locations on the main stems and presents data used in the case study. At the same time, this paper also compares several different network architectures for main stem detection and segmentation and selects YOLOv5 and PSPNet as the most promising models for main stem detection and segmentation tasks, respectively. The workflow combines deep learning and traditional image processing algorithms to calculate the accurate location information of litchi main stem picking points in the litchi image. This workflow takes YOLOv5 as the target detection model to detect the litchi main stem in the litchi image, then extracts the detected region of interest (ROI) of the litchi main stem, uses PSPNet semantic segmentation model to semantically segment the ROI image of the main stem, carries out image post-processing operation on the ROI image of the main stem after semantic segmentation, and obtains the pixel coordinates of picking points in the ROI image of the main stem. After coordinate conversion, the pixel coordinates of the main stem picking points of the original litchi image are obtained, and the picking points are drawn on the litchi image. At present, the workflow can obtain the accurate position information of the main stem picking point in the litchi image. The recall and precision of this method were 76.29% and 92.50%, respectively, which lays a foundation for the subsequent work of obtaining the three-dimensional coordinates of the main stem picking point according to the image depth information, even though we have not done this work in this paper.

Cadmium stress is a major threat to plant growth and survival worldwide. The current study aims to green synthesis, characterization, and application of zinc-oxide nanoparticles to alleviate cadmium stress in maize (Zea mays L.) plants. In this experiment, two cadmium levels (0, 0.6 mM) were applied to check the impact on plant growth attributes, chlorophyll contents, and concentration of various primary metabolites and antioxidants under exogenous treatment of zinc-oxide nanoparticles (25 and 50 mg L^-1) in maize seedlings. Tissue sampling was made 21 days after the zinc-oxide nanoparticles application. Our results showed that applying cadmium significantly reduced total chlorophyll and carotenoid contents by 52.87% and 23.31% compared to non-stress. In comparison, it was increased by 53.23%, 68.49% and 9.73%, 37.53% with zinc-oxide nanoparticles 25, 50 mg L^-1 application compared with cadmium stress conditions, respectively. At the same time, proline, superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase contents were enhanced in plants treated with cadmium compared to non-treated plants with no foliar application, while it was increased by 12.99 and 23.09%, 23.52 and 35.12%, 27.53 and 36.43%, 14.19 and 24.46%, 14.64 and 37.68% by applying 25 and 50 mg L^-1 of zinc-oxide nanoparticles dosages, respectively. In addition, cadmium toxicity also enhanced stress indicators such as malondialdehyde, hydrogen peroxide, and non-enzymatic antioxidants in plant leaves. Overall, the exogenous application of zinc-oxide nanoparticles (25 and 50 mg L^-1) significantly alleviated cadmium toxicity in maize. It provides the first evidence that zinc-oxide nanoparticles 25 ~ 50 mg L^-1 can be a candidate agricultural strategy for mitigating cadmium stress in cadmium-polluted soils for safe agriculture practice.

Background: Unmanned Aerial Vehicles (UAVs) applied to agricultural plant protection is widely used, and the field of operation is expanding due to their high efficiency and pesticide application reduction. However, the work on pesticide drift lags behind the development of the UAV spraying device. Methods: We compared the spray drift potential at four liquid pressures of 2, 3, 4, and 5 bar ejected from the hydraulic nozzles mounted on a UAV test platform exposed to different wind speeds of 2, 4, and 6 m/s produced by a wind tunnel. The combination of the wind tunnel and the UAV test platform was used to obtain strict test conditions. The droplet size distribution under spray drift pressures was measured by a laser diffraction instrument. Results: Increasing the pressure leads to smaller droplet volume diameters and produced fine droplets of less than 100 µm. The deposition in the drift area was elevated at most of the sampling locations by setting higher pressure and faster wind speed. The deposition ratios were all higher than the flow ratios under three wind speeds after the adjustment of pressures. For most samples within a short drift distance (2–8 m), the drift with the rotor motor off was more than an order of magnitude higher than that with the rotor motor on at a pressure of 3 bar. Conclusions: In this study, the wind speed and liquid pressure all had a significant effect on the UAV spray drift, and the rotor wind significantly inhibited a large number of droplets from drifting further.

Plant protection drone spraying technology is widely used to prevent and control crop diseases and pests due to its advantages of being unaffected by crop growth patterns and terrain restrictions, high operational efficiency, and low labor requirements. The operational parameters of plant protection drones significantly impact the distribution of spray droplets, thereby affecting pesticide utilization. In this study, a field experiment was conducted to determine the working modes of two representative plant protection drones and an electric backpack sprayer as a control to explore the characteristics of droplet deposition with different spray volumes in the citrus canopy. The results showed that the spraying volume significantly affected the number of droplets and the spray coverage. The number of droplets and the spray coverage area on the leaf surface were significantly increased by increasing the spray volume from 60 L/ha to 120 L/ha in plant protection drones. Particularly for the DJI T30, the mid-lower canopy showed a spray coverage increase of 52.5%. The droplet density demonstrated the most significant variations in the lower inner canopy, ranging from 18.7 droplets/cm² to 41.7 droplets/cm² by XAG V40. From the deposition distribution on fruit trees, the plant protection drones exhibit good penetration ability, as the droplets can achieve a relatively even distribution in different canopy layers of citrus trees. The droplet distribution uniformity inside the canopy is similar for XAG V40 and DJI T30, with a variation coefficient of approximately 50%-100%. Compared to the plant protection drones, the knapsack electric sprayer is suitable for pest and disease control in the mid-lower canopy, but they face challenges of insufficient deposition capability in the upper canopy and overall poor spray uniformity. The distribution of deposition determined in this study provides data support for the selection of spraying agents for fruit trees by plant protection drones and for the control of different pests and diseases.

The use of drones in agriculture is expanding at a brisk pace in crop production due to the superiority in precision, efficiency, and safety of their applicators. However, their potential drift risk also raises concern for users and regulatory authorities. The method of wind tunnel research can effectively evaluate the weighted influence of each drift factor, especially the drift characteristics of the nozzle and spray solution. Based on the wind tunnel test results, centrifugal nozzles have a higher drift risk than hydraulic nozzles, even with a similar DV₅₀. The cumulative drift rate of the centrifugal nozzle at 2 m downwind was 90.1% compared to the LU12001 nozzle’s 40.6% under the wind speed of 3.5 m/s. Compared with the same coding as the flat fan hydraulic nozzle, the IDK nozzle can effectively reduce the drift rate. For the tested nozzles, DV₅₀ and wind speed had a linear relationship with drift rate, and the sampling location had an exponential or logarithmic relationship with drift rate. Spray adjuvants, especially modified vegetable oils, had a significant effect on reducing the amount of drift. The results of this experiment provide a reference for the selection of nozzles and the addition of spray adjuvants. Further clarifying the spray drift characteristics of drones until a drift prediction model is available is still the focus of research.