In plants, the Gretchen GH3 (GH3) protein is involved in free auxin (IAA) and amino acid conjugation, thus controlling auxin homeostasis. To date, many GH3 gene families have been identified from different plant species. However, the GH3 gene family in kiwifruit (Actinidia chinensis) has not been reported. In this study, 12 AcGH3 genes were identified, phylogenetic analysis of AtGH3 (Arabidopsis), SlGH3 (Solanum lycopersicum), and AcGH3 provided insights into various orthologous relationships among these proteins, which were categorized into three groups. Expression analysis of AcGH3 genes at different postharvest stages suggested limited or no role for most of the AcGH3 genes at the initiation of fruit ripening. AcGH3.1 was the only gene exhibiting ripening-associated expression. Further study showed that the expression of AcGH3.1 gene was induced by NAA (1-naphthylacetic acid, auxin analogue) and inhibited by 1-MCP (1-methylcyclopropene, ethylene receptor inhibitor), respectively. AcGH3.1 gene silencing inhibited gene expression and delayed fruit softening in kiwifruit. The results indicate that AcGH3.1 may play an important role in the softening process of fruits. Analysis of the AcGH3.1 promoter revealed the presence of many cis-elements related to hormones, light, and drought. The determination of GUS (β-Galactosidase) enzyme activity revealed that promoter activity increased strikingly upon abscisic acid (ABA), ethylene, or NAA treatment, and significantly decreased with salicylic acid (SA) treatment. The present study could help in the identification of GH3 genes and revelation of AcGH3.1 gene function during postharvest stages, which pave the way for further functional verification of the AcGH3.1 gene.

Sucrose is an important component of fruit flavor, but whether sucrose signaling affects the postharvest ripening process of kiwifruit is unclear. The aim of this article was to study the effect of sucrose application on postharvest kiwifruit ripening to clarify the effect of sucrose in this process. Our present study found that exogenous sucrose can promote ethylene synthesis, which increases the ethylene content during fruit ripening, thereby accelerating the ripening and softening of kiwifruit after harvest. A significantly higher expression of AcACS1 and AcACO2 was found in sucrose-treated fruits compared to that in mannitol-treated fruits. Blocking the ethylene signal significantly inhibited the sucrose-modulated expression of most selected ripening-related genes. Sucrose transport is essential for sucrose accumulation in fruits; therefore, we isolated the gene family related to sucrose transport in kiwifruit and analyzed the gene expression of its members. The results show that AcSUT1 and AcTST1 expression increased with fruit ripening and AcSUT4 expression decreased with ripening, indicating that they may have different roles in the regulation of fruit ripening. Additionally, many cis-elements associated with phytohormones and sugar responses were found in the promoter of the three genes, which suggests that they were transcriptionally regulated by sugar signal and phytohormones. This study demonstrates the effect of sucrose on postharvest ripening of kiwifruit, providing a good foundation for further research.