Photosynthetic pigments and relative water content of young leaves of P. fruticosa decreased considerably with the onset of the summer dry period and stabilized at low values for the last two summer months, while leaf growth was arrested. Corresponding decreases in photochemical efficiency of photosystem II, as judged by chlorophyll fluorescence measurements of predarkened leaves, were, however, negligible. Following the first autumn heavy rains, growth was restored and photosynthetic pigments and relative water content increased to the pre-drought values. The results indicate that the reduction of chlorophylls does not result from severe photoinhibitory damage but, instead, it may be an adaptive response against the adverse conditions of the Mediterranean summer. Some photosynthetic and photoprotective characteristics of P. fruticosa leaves at two stages of their development, i.e. at the severely dehydrated state with arrested growth during late summer and after their revival following the first heavy autumn rains were compared. Apart from the chlorophyll loss, the photon yield of O₂ evolution and the photosynthetic capacity at saturated CO₂ were considerably suppressed during the summer, indicating that the extremely low net photosynthetic rates observed in the field were the combined result of stomatal and mesophyll limitations. Epoxidation state was low at midday during the summer, indicating an active, photodissipative xanthophyll cycle. Although zeaxanthin content did not increase at midday after the rains, the potential of the cycle was maintained in the revived leaves, as judged by the high concentrations of the cycle components. After the rains, the activities of the anti-oxidant enzymes (superoxide dismutase, ascorbate peroxidase) remained relatively unchanged on a chlorophyll basis, but increased when expressed on a leaf surface area or protein basis. It may be concluded that P. fruticosa leaves avoid severe photoinhibitory and oxidative damage during the long, warm, dry and sunny Mediterranean summer by reducing light harvesting and electron flow capacity, whilst maintaining an adequate photoprotective ability. The preservation of a remarkable photodissipative and anti-oxidative potential after the rains may be related to the low predictability of precipitation even during the rainy winter.

Prunus domestica L. has green leaves, whereas Prunus cerasifera Ehrh. var. atropurpurea has red leaves due to the presence of mesophyll anthocyanins. We compared morphological and photosynthetic characteristics of leaves of these species, which were sampled from shoots grafted in pairs on P. domestica rootstocks, each pair comprising one shoot of each species. Two hypotheses were tested: (1) anthocyanins protect red leaves against photoinhibition; and (2) red leaves display shade characteristics because of light attenuation by anthocyanins. Parameters were measured seasonally, during a period of increasing water stress, which caused a similar drop in shoot water potential in each species. As judged by predawn measurements of maximum PSII yield, chronic photoinhibition did not develop in either species and, despite the anthocyanic screen, the red leaves of P. cerasifera displayed lower light-adapted PSII yields and higher non-photochemical quenching than the green leaves of P. domestica. Thus, it appears that, in this system, anthocyanins afford little photoprotection. As predicted by the shade acclimation hypothesis, red leaves were thinner and had a lower stomatal frequency, area- based CO₂ assimilation rate, apparent carboxylation efficiency and chlorophyll a:b ratio than green leaves. However, red leaves were similar to green leaves in conductivity to water vapor diffusion, dry-mass-based chlorophyll concentrations and carotenoid:chlorophyll ratios. The data for red leaves indicate adaptations to a green-depleted, red-enriched shade, rather than a neutral or canopy-like shade. Thus, green light attenuation by anthocyanins may impose a limitation on leaf thickness. Moreover, the selective depletion of light at wavelengths that are preferentially absorbed by PSII and chlorophyll b may lead to adjustments in chlorophyll pigment ratios to compensate for the uneven spectral distribution of internal light. The apparent photosynthetic cost associated with lost photons and reduced leaf thickness, and the absence of a photoprotective advantage, suggest that there are other, yet to be identified, benefits for permanently anthocyanic leaves of P. cerasifera.