Long-term structural health monitoring (SHM) plays an important role in the safety of public transport infrastructure such as bridges or tunnels and warns in the event of any emerging problem. This article describes development and testing of system based on fiber Bragg grating (FBG) sensors that can detect changes in strain and temperature. The first phase of the research has been focused on the development of new fiber optic sensors for the monitoring of concrete structures and their investigation in laboratory conditions. The work also shows novel applicability of the same FBG technology for glulam structures. Mechanical loading tests of the concrete beam as well as glulam beam with embedded sensors were carried out. Data measured by developed fiber optic sensors were compared with the readings from reference sensors as well as with the analytically calculated values. The achieved results proved good agreement between the measured data, analytical data and reference methods. In second phase of the research, the pilot installation of the sensors was carried out on the newly constructed prestressed-concrete bridge. The bridge was monitored throughout pre-stressing phase and monitoring continued after the completion of the construction works. Problems with the fragility of the sensors occurred during the measurements, but the obtained results provide a good basis for further improvement of the system.