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Fruit used in the common human diet in general, and kiwifruit and persimmon particularly, displays health properties in the prevention of heart disease. This study describes a combination of bioactivity, multivariate data analyses and fluorescence measurements for the differentiating of kiwifruit and persimmon, their quenching and antioxidant properties. The metabolic differences are shown, as well in the results of bioactivities and antioxidant capacities determined by ABTS, FRAP, CUPRAC and DPPH assays. To complement the bioactivity of these fruits, the quenching properties between extracted polyphenols and human serum proteins were determined by 3D-fluorescence spectroscopy studies. These properties of the extracted polyphenols in interaction with the main serum proteins in the human metabolism (human serum albumin (HSA), α-β-globulin (α-β G) and fibrinogen (Fgn)), showed that kiwifruit was more reactive than persimmon. There was a direct correlation between the quenching properties of the polyphenols of the investigated fruits with serum human proteins, their relative quantification and bioactivity. The results of metabolites and fluorescence quenching show that these fruits possess multiple properties that have a great potential to be used in industry with emphasis on the formulation of functional foods and in the pharmaceutical industry. Based on the quenching properties of human serum proteins with polyphenols and recent reports in vivo on human studies, we hypothesize that HSA, α-β G and Fgn will be predictors of coronary artery disease (CAD).
Young-Mo Kim; Faridah Abas; Yong-Seo Park; Yang-Kyun Park; Kyung-Sik Ham; Seong-Gook Kang; Martyna Lubinska-Szczygeł; Aviva Ezra; Shela Gorinstein. Bioactivities of Phenolic Compounds from Kiwifruit and Persimmon. Molecules 2021, 26, 4405 .
AMA StyleYoung-Mo Kim, Faridah Abas, Yong-Seo Park, Yang-Kyun Park, Kyung-Sik Ham, Seong-Gook Kang, Martyna Lubinska-Szczygeł, Aviva Ezra, Shela Gorinstein. Bioactivities of Phenolic Compounds from Kiwifruit and Persimmon. Molecules. 2021; 26 (15):4405.
Chicago/Turabian StyleYoung-Mo Kim; Faridah Abas; Yong-Seo Park; Yang-Kyun Park; Kyung-Sik Ham; Seong-Gook Kang; Martyna Lubinska-Szczygeł; Aviva Ezra; Shela Gorinstein. 2021. "Bioactivities of Phenolic Compounds from Kiwifruit and Persimmon." Molecules 26, no. 15: 4405.
Durian (Durio zibethinus Murr.), like many other exotic, tropical, and conventional fruits, is important in the prevention of different diseases. In this study, the characterization of the main bioactive compounds of the most popular cultivars of durian and their properties are described. The changes in the quality indices of the antioxidant status were determined by CUPRAC, ABTS, FRAP, DPPH, and ORAC assays. The profiling of phytochemicals was carried out by Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). For the first time, in vitro studies were performed by the interaction of extracted durian polyphenols with human serum proteins (HSP) such as human serum albumin (HSAlb), fibrinogen (HSFib) and globulin (HSGlo) as novel biomarkers of coronary artery disease (CAD). The fluorescence measurements of the resulting intensity and calculated binding properties of the interaction of polyphenols with proteins showed that the most reactive was Monthong durian cultivar. This study suggests that durian cultivars have relatively strong antioxidant, binding, and health potentials and could be a significant source of natural antioxidants used in daily fresh consumption and for functional foods.
Supeeraya Arsa; Angkana Wipatanawin; Rachit Suwapanich; Orachorn Makkerdchoo; Niphattha Chatsuwan; Pensiri Kaewthong; Praphan Pinsirodom; Ruchira Taprap; Ratiporn Haruenkit; Sumitra Poovarodom; Martyna Lubinska-Szczygeł; Elena Katrich; Shela Gorinstein. Properties of Different Varieties of Durian. Applied Sciences 2021, 11, 5653 .
AMA StyleSupeeraya Arsa, Angkana Wipatanawin, Rachit Suwapanich, Orachorn Makkerdchoo, Niphattha Chatsuwan, Pensiri Kaewthong, Praphan Pinsirodom, Ruchira Taprap, Ratiporn Haruenkit, Sumitra Poovarodom, Martyna Lubinska-Szczygeł, Elena Katrich, Shela Gorinstein. Properties of Different Varieties of Durian. Applied Sciences. 2021; 11 (12):5653.
Chicago/Turabian StyleSupeeraya Arsa; Angkana Wipatanawin; Rachit Suwapanich; Orachorn Makkerdchoo; Niphattha Chatsuwan; Pensiri Kaewthong; Praphan Pinsirodom; Ruchira Taprap; Ratiporn Haruenkit; Sumitra Poovarodom; Martyna Lubinska-Szczygeł; Elena Katrich; Shela Gorinstein. 2021. "Properties of Different Varieties of Durian." Applied Sciences 11, no. 12: 5653.
Dragon fruits are a valued source of bioactive compounds with high potential to become a functional food. The aim of the study was to evaluate and compare the chemopreventive potential and chemical composition of fruits harvested in Thailand and Israel. The amount of different compounds in water and methanol extracts and antioxidant activity was investigated. Moreover, cytotoxic activity against cancer and normal cells of skin, prostate, and gastrointestinal origin was performed, accompanied by anti-inflammatory assay based on NO production in RAW 264.7 macrophage model. Additionally, the quenching properties of polyphenols from fruits were determined by the interaction of the main drug carrier in blood human serum (HSA). The chemometric analysis was used to reveal the relationships between the determined parameters. Dragon fruits harvested in Israel revealed higher antioxidant properties and total content of polyphenols and betacyanins when compared to those from Thailand. The examined fruits of both origins showed significant cytotoxic activity toward colon and prostate cancer cells, with no toxic effect on normal cells, but also no anti-inflammatory effect. Moreover, a high binding ability to HSA was observed for water extracts of dragon fruits. All these predestine dragon fruits are the candidates for the attractive and chemopreventive elements of daily diet.
Paweł Paśko; Agnieszka Galanty; Paweł Zagrodzki; Patraporn Luksirikul; Dinorah Barasch; Alina Nemirovski; Shela Gorinstein. Dragon Fruits as a Reservoir of Natural Polyphenolics with Chemopreventive Properties. Molecules 2021, 26, 2158 .
AMA StylePaweł Paśko, Agnieszka Galanty, Paweł Zagrodzki, Patraporn Luksirikul, Dinorah Barasch, Alina Nemirovski, Shela Gorinstein. Dragon Fruits as a Reservoir of Natural Polyphenolics with Chemopreventive Properties. Molecules. 2021; 26 (8):2158.
Chicago/Turabian StylePaweł Paśko; Agnieszka Galanty; Paweł Zagrodzki; Patraporn Luksirikul; Dinorah Barasch; Alina Nemirovski; Shela Gorinstein. 2021. "Dragon Fruits as a Reservoir of Natural Polyphenolics with Chemopreventive Properties." Molecules 26, no. 8: 2158.
Kohlrabi sprouts are just gaining popularity as the new example of functional food. The study was focused on the influence of germination time and light conditions on glucosinolates, phenolic acids, flavonoids, and fatty acids content in kohlrabi sprouts, in comparison to the bulbs. The effect of kohlrabi products on SW480, HepG2 and BJ cells was also determined. The length of sprouting time and light availability significantly influenced the concentrations of the phenolic compounds. Significant differences in progoitrin concentrations were observed between the sprouts harvested in light and in the darkness, with significantly lower content for darkness conditions. Erucic acid was the dominant fatty acid found in sprouts (14.5–34.5%). Sprouts and bulbs were less toxic to normal than to cancer cells. The sprouts stimulated necrosis (56.4%) more than apoptosis (34.1%) in SW480 cells, while the latter effect was predominant for the bulbs. Both sprouts and bulbs caused rather necrosis (45.5 and 63.9%) than apoptosis (32 and 32.5%) in HepG2 cells.
Paweł Paśko; Agnieszka Galanty; Małgorzata Tyszka-Czochara; Paweł Żmudzki; Paweł Zagrodzki; Joanna Gdula-Argasińska; Ewelina Prochownik; Shela Gorinstein. Health Promoting vs Anti-nutritive Aspects of Kohlrabi Sprouts, a Promising Candidate for Novel Functional Food. Plant Foods for Human Nutrition 2021, 76, 76 -82.
AMA StylePaweł Paśko, Agnieszka Galanty, Małgorzata Tyszka-Czochara, Paweł Żmudzki, Paweł Zagrodzki, Joanna Gdula-Argasińska, Ewelina Prochownik, Shela Gorinstein. Health Promoting vs Anti-nutritive Aspects of Kohlrabi Sprouts, a Promising Candidate for Novel Functional Food. Plant Foods for Human Nutrition. 2021; 76 (1):76-82.
Chicago/Turabian StylePaweł Paśko; Agnieszka Galanty; Małgorzata Tyszka-Czochara; Paweł Żmudzki; Paweł Zagrodzki; Joanna Gdula-Argasińska; Ewelina Prochownik; Shela Gorinstein. 2021. "Health Promoting vs Anti-nutritive Aspects of Kohlrabi Sprouts, a Promising Candidate for Novel Functional Food." Plant Foods for Human Nutrition 76, no. 1: 76-82.
Pitaya or dragon fruit comprises a group of exotic cacti species of the genus Hylocereus, with characteristic, eye-catching fruits. The present study analyzed the bioactive compounds (flavonoids, phenolic acids, total polyphenols and betacyanins) in different species (H. costaricensis, H. undatus and H. megalanthus) and varieties of fruits harvested in Israel. Direct comparisons of antioxidant and cytotoxic properties of the methanol and water extracts of the fruits, with the range of antioxidant tests (ABTS, CUPRAC, DPPH and FRAP) and also with normal (HaCaT and PNT2) and cancer (A375, WM793, Du145, HepG2, Caco-2, HT-29 and A549) cell lines was done. Advanced chemometric analysis was used to provide more information on the relationship between the fruits’ compounds, varieties and biological activity of pitaya. Significant differences were observed between the tested pitaya samples for their chemical content and bioactivities. Red-skin pitaya fruits had the highest content of polyphenols and betacyanins and also had high antioxidant activity. The cytotoxic effect of H. costaricensis on cancer cells was greater than that of H. megalanthus and H. undatus, while none of the species was toxic to normal cells. The results showed that pitaya fruits have the potential to be included among the functional fruits. The species and even the variety of the pitaya fruit are of importance, as the different fruits varied significantly in their properties.
Paweł Paśko; Agnieszka Galanty; Paweł Zagrodzki; Yang Gyu Ku; Patraporn Luksirikul; Moshe Weisz; Shela Gorinstein. Bioactivity and cytotoxicity of different species of pitaya fruits – A comparative study with advanced chemometric analysis. Food Bioscience 2021, 40, 100888 .
AMA StylePaweł Paśko, Agnieszka Galanty, Paweł Zagrodzki, Yang Gyu Ku, Patraporn Luksirikul, Moshe Weisz, Shela Gorinstein. Bioactivity and cytotoxicity of different species of pitaya fruits – A comparative study with advanced chemometric analysis. Food Bioscience. 2021; 40 ():100888.
Chicago/Turabian StylePaweł Paśko; Agnieszka Galanty; Paweł Zagrodzki; Yang Gyu Ku; Patraporn Luksirikul; Moshe Weisz; Shela Gorinstein. 2021. "Bioactivity and cytotoxicity of different species of pitaya fruits – A comparative study with advanced chemometric analysis." Food Bioscience 40, no. : 100888.
Dragon fruit has appealed much concern from consumers as a novelty fruit with potent nutritional and medicinal benefits. Dragon fruit quality warrants comprehensive evaluation, based on the contents of pigments and health-promoting natural compounds in different varieties. This study was aimed to evaluate the differences among dragon fruit varieties extracted with methanol–water (CD3OD-D2O) and methanol (CD3OD) by proton nuclear magnetic resonance (1H NMR)-based metabolomics approach. The variation features of the metabolite profiles were studied between varieties and origins of dragon fruit, considering the differences in principal component analysis (PCA). The hierarchical clustering analysis (HCA) based on score values of PCA model was also performed to analyze the distance between samples based on metabolites contents. The results of 1H NMR spectra showed that the CD3OD-D2O extracts quantitatively differ from CD3OD ones. In dragon fruit extracts, 36 metabolites were identified. The results demonstrated that the methanol and methanol/water extracted similar compounds with higher intensity in methanol. The metabolic differences among varieties were also shown for CD3OD extracts by comparing both Pareto and UV scaling methods. The big size red fleshed dragon fruit (samples 2 and 3), growing in Israel were clustered similar to that growing in Thailand with the abundance of phenolic compounds. Glucose and fructose were more prominent in the yellow and white fleshed fruit (samples 4 and 5) growing in Israel. To support the obtained results two dimensional 1H–1H J-resolved and UHPLC-MS measurements were carried out. This research gain novel insights into the field as the first NMR metabolites fingerprinting of the major dragon fruit varieties. The correlations between DPPH, CUPRAC, antioxidant and metabolomic properties were also evaluated. The chemical markers associated with varieties of dragon fruit quality and their appearances were identified and can be utilized for the basis of authentication purpose of this fruit.
Nabil Ali Al-Mekhlafi; Ahmed Mediani; Nor Hadiani Ismail; Faridah Abas; Tomasz Dymerski; Martyna Lubinska-Szczygeł; Suchada Vearasilp; Shela Gorinstein. Metabolomic and antioxidant properties of different varieties and origins of Dragon fruit. Microchemical Journal 2020, 160, 105687 .
AMA StyleNabil Ali Al-Mekhlafi, Ahmed Mediani, Nor Hadiani Ismail, Faridah Abas, Tomasz Dymerski, Martyna Lubinska-Szczygeł, Suchada Vearasilp, Shela Gorinstein. Metabolomic and antioxidant properties of different varieties and origins of Dragon fruit. Microchemical Journal. 2020; 160 ():105687.
Chicago/Turabian StyleNabil Ali Al-Mekhlafi; Ahmed Mediani; Nor Hadiani Ismail; Faridah Abas; Tomasz Dymerski; Martyna Lubinska-Szczygeł; Suchada Vearasilp; Shela Gorinstein. 2020. "Metabolomic and antioxidant properties of different varieties and origins of Dragon fruit." Microchemical Journal 160, no. : 105687.
Our recently published in vivo studies and growing evidence suggest that moderate consumption of beer possesses several health benefits, including antioxidant and cardiovascular effects. Although beer contains phenolic acids and flavonoids as the major composition, and upon consumption, the levels of major components increase in the blood, there is no report on how these beer components interact with main human serum proteins. Thus, to address the interaction potential between beer components and human serum proteins, the present study primarily aims to investigate the components of beer from different industrial sources as well as their mode of interaction through in silico analysis. The contents of the bioactive compounds, antioxidant capacities and their influence on binding properties of the main serum proteins in human metabolism (human serum albumin (HSA), plasma circulation fibrinogen (PCF), C-reactive protein (CRP) and glutathione peroxidase 3 (GPX3)) were studied. In vitro and in silico studies indicated that phenolic substances presented in beer interact with the key regions of the proteins to enhance their antioxidant and health properties. We hypothesize that moderate consumption of beer could be beneficial for patients suffering from coronary artery disease (CAD) and other health advantages by regulating the serum proteins.
Raja Shafreen; Selvaraj Lakshmi; Shunmugiah Pandian; Yong Park; Young Kim; Paweł Paśko; Joseph Deutsch; Elena Katrich; Shela Gorinstein. Unraveling the Antioxidant, Binding and Health-Protecting Properties of Phenolic Compounds of Beers with Main Human Serum Proteins: In Vitro and In Silico Approaches. Molecules 2020, 25, 4962 .
AMA StyleRaja Shafreen, Selvaraj Lakshmi, Shunmugiah Pandian, Yong Park, Young Kim, Paweł Paśko, Joseph Deutsch, Elena Katrich, Shela Gorinstein. Unraveling the Antioxidant, Binding and Health-Protecting Properties of Phenolic Compounds of Beers with Main Human Serum Proteins: In Vitro and In Silico Approaches. Molecules. 2020; 25 (21):4962.
Chicago/Turabian StyleRaja Shafreen; Selvaraj Lakshmi; Shunmugiah Pandian; Yong Park; Young Kim; Paweł Paśko; Joseph Deutsch; Elena Katrich; Shela Gorinstein. 2020. "Unraveling the Antioxidant, Binding and Health-Protecting Properties of Phenolic Compounds of Beers with Main Human Serum Proteins: In Vitro and In Silico Approaches." Molecules 25, no. 21: 4962.
The current study attempted to understand the interaction profiles of phytoconstituents in new and traditionally used fruit cultivars with human serum albumin (HSA) in the context of predicting the biological role under in vivo conditions. Therefore, polyphenols, flavonoids, flavanols, tannins, vitamin C, secondary metabolites and their antioxidant capacities of organic kiwifruit Actinidia (A.) eriantha cv. Bidan (AEB) and A. arguta cv. Cheongsan (AAC), as new cultivars grown in Korea, and widely consumed A. deliciosa cv. Hayward (ADH) and Diospyros kaki Thunb. cv. Fuyu (DKF) were determined and compared. All investigated fruits showed relatively high antioxidant capacities. To complement the bioactivity of these fruits, the binding properties between extracted polyphenols and HSA were determined by 3D-fluorescence spectroscopy and docking studies. The most bioactive was AEB with the highest percentage of binding, following by AAC, ADH and DKF. Our study for the first time unveils the differential binding properties of kiwifruit and persimmon phytoconstituents with HSA. Although cultivars possess virtually the same phytoconstituents, presence of one unique compound significantly alters the binding properties of HSA. The results of fluorescence quenching and molecular docking showed that these fruits possess multiple properties, which have a great potential to be used in industry with emphasis on the formulation of functional foods and medicinal applications.
Young Mo Kim; Yong Seo Park; Yang Kyun Park; Kyung Sik Ham; Seong Gook Kang; Raja Mohamed Beema Shafreen; Selvaraj Alagu Lakshmi; Shela Gorinstein. Characterization of Bioactive Ligands with Antioxidant Properties of Kiwifruit and Persimmon Cultivars Using In Vitro and in Silico Studies. Applied Sciences 2020, 10, 4218 .
AMA StyleYoung Mo Kim, Yong Seo Park, Yang Kyun Park, Kyung Sik Ham, Seong Gook Kang, Raja Mohamed Beema Shafreen, Selvaraj Alagu Lakshmi, Shela Gorinstein. Characterization of Bioactive Ligands with Antioxidant Properties of Kiwifruit and Persimmon Cultivars Using In Vitro and in Silico Studies. Applied Sciences. 2020; 10 (12):4218.
Chicago/Turabian StyleYoung Mo Kim; Yong Seo Park; Yang Kyun Park; Kyung Sik Ham; Seong Gook Kang; Raja Mohamed Beema Shafreen; Selvaraj Alagu Lakshmi; Shela Gorinstein. 2020. "Characterization of Bioactive Ligands with Antioxidant Properties of Kiwifruit and Persimmon Cultivars Using In Vitro and in Silico Studies." Applied Sciences 10, no. 12: 4218.
In the presented study, an overall Jaffa sweetie evaluation was made to find a correlation between Citrus grandis Osbeck × Citrus paradisi Macf. and its parent fruits’ (Citrus grandis Osbeck, Citrus paradisi Macf.) properties. Based on the sensory analysis, it was found that the taste and aroma of the new hybrid fruit are close to pummelo. By the use of chromatographic analysis, the selected monoterpenes present in the fruits were quantified. α-terpineol was typed as the main monoterpene compound in the headspace of sweetie and grapefruit, with the concentrations: 20.96 and 87.9 μg/g, respectively. In turn, γ-terpinene was chosen as the most important monoterpene determining the flavor of sweetie fruit. Based on two-dimensional gas chromatography (GC × GC-TOF-MS) and principal component analysis (PCA) of the data, several volatile compounds were associated with analyzed fruits’ aroma. Jaffa Sweetie is the hybrid fruit with sensory properties similar to pummelo with a higher content of monoterpenes, which improves its health benefits compared to the parent fruit. The research presents an instrumental method for assessing the aroma properties of the fruit as a reference method for sensory analysis, commonly used in the industry.
Martyna Lubinska-Szczygeł; Żaneta Polkowska; Tomasz Dymerski; And Shela Gorinstein. Comparison of the Physical and Sensory Properties of Hybrid Citrus Fruit Jaffa® Sweetie in Relation to the Parent Fruits. Molecules 2020, 25, 1 .
AMA StyleMartyna Lubinska-Szczygeł, Żaneta Polkowska, Tomasz Dymerski, And Shela Gorinstein. Comparison of the Physical and Sensory Properties of Hybrid Citrus Fruit Jaffa® Sweetie in Relation to the Parent Fruits. Molecules. 2020; 25 (12):1.
Chicago/Turabian StyleMartyna Lubinska-Szczygeł; Żaneta Polkowska; Tomasz Dymerski; And Shela Gorinstein. 2020. "Comparison of the Physical and Sensory Properties of Hybrid Citrus Fruit Jaffa® Sweetie in Relation to the Parent Fruits." Molecules 25, no. 12: 1.
Bioactivities of persimmon and kiwifruit were studied and compared in connection with their characteristics towards human health. Based on the results of our recently published research, two fruits growing in South Korea persimmon ‘Fuyu’ and kiwifruit ‘Hayward’ were investigated and compared. Spectroscopic, fluorescence and FTIR measurements and radical scavenging reactions were used to compare the qualities of persimmon and kiwifruit. The results of above methods showed that total polyphenol and tannin substances, vitamin C and values of antioxidant capacities were higher in kiwifruit than in persimmon. Phenolics were also characterized by specific IR bands and MS spectra. Antioxidant capacities of polyphenols extracted from the studied fruits correlated with the binding abilities during their interaction with serum albumin, one of the most important proteins in human metabolism. As a result of a complex reaction between the proteins and polyphenols, the fluorescence intensity of proteins decreased and such interaction can be a model in distribution of drugs. Relationship between the most active compounds and their influence on the quality of fruits was established, based on the relatively high bioactivity and quenching properties. In conclusion, kiwifruit and persimmon according to the obtained results contain high amounts of antioxidants and can be widely consumed as fresh, processed and functional foods.
Young Mo Kim; Yong Seo Park; Yang Kyun Park; Kyung Sik Ham; Seong Gook Kang; Dinorah Barasch; Alina Nemirovski; Shela Gorinstein. Phytochemical analysis of two main varieties of persimmon and kiwifruit and their antioxidative and quenching capacities. European Food Research and Technology 2020, 246, 1259 -1268.
AMA StyleYoung Mo Kim, Yong Seo Park, Yang Kyun Park, Kyung Sik Ham, Seong Gook Kang, Dinorah Barasch, Alina Nemirovski, Shela Gorinstein. Phytochemical analysis of two main varieties of persimmon and kiwifruit and their antioxidative and quenching capacities. European Food Research and Technology. 2020; 246 (6):1259-1268.
Chicago/Turabian StyleYoung Mo Kim; Yong Seo Park; Yang Kyun Park; Kyung Sik Ham; Seong Gook Kang; Dinorah Barasch; Alina Nemirovski; Shela Gorinstein. 2020. "Phytochemical analysis of two main varieties of persimmon and kiwifruit and their antioxidative and quenching capacities." European Food Research and Technology 246, no. 6: 1259-1268.
The present study showed the bioactive properties such as antioxidant, quenching, structural and antifungal in six Amaranthus (A.) hypochondriacus laboratory and industrial products. The values of CUPRAC radical scavenging, quenching and antifungal capacities in industrial samples were higher than in laboratory. Such properties can be explained by the presence of polyphenols in the samples. Higher molecular weights and corresponding protein bands separated sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS–PAGE) were observed in laboratory protein isolates rather than in industrial samples. Interaction with human serum albumin (HSA) was used to determine the functional properties of the products. As characterization of antifungal properties, all amaranth products at 30 μg/mL concentration significantly reduced (<50% inhibition) the metabolic activity of Candida (C.) albicans cells within the biofilm. The structural studies of proteins by FTIR, fluorescence and UV spectroscopy with SDS denaturation showed that after the industrial treatment the proteins were relatively stable. The investigated proteins could be considered as an indicator of food control in commercial industrial products and a source of food additives with health benefits.
María A. Lozano-Grande; Alma Leticia Martínez-Ayala; Rajamohamed Beema Shafreen; Arkadiusz Szterk; Zenon Jastrzębski; Hanna Leontowicz; Jerzy Drzewiecki; Pawel Pasko; Aviva Ezra; Shela Gorinstein. Antioxidant, quenching, electrophoretic, antifungal and structural properties of proteins and their abilities to control the quality of Amaranthus industrial products. Food Control 2020, 115, 107276 .
AMA StyleMaría A. Lozano-Grande, Alma Leticia Martínez-Ayala, Rajamohamed Beema Shafreen, Arkadiusz Szterk, Zenon Jastrzębski, Hanna Leontowicz, Jerzy Drzewiecki, Pawel Pasko, Aviva Ezra, Shela Gorinstein. Antioxidant, quenching, electrophoretic, antifungal and structural properties of proteins and their abilities to control the quality of Amaranthus industrial products. Food Control. 2020; 115 ():107276.
Chicago/Turabian StyleMaría A. Lozano-Grande; Alma Leticia Martínez-Ayala; Rajamohamed Beema Shafreen; Arkadiusz Szterk; Zenon Jastrzębski; Hanna Leontowicz; Jerzy Drzewiecki; Pawel Pasko; Aviva Ezra; Shela Gorinstein. 2020. "Antioxidant, quenching, electrophoretic, antifungal and structural properties of proteins and their abilities to control the quality of Amaranthus industrial products." Food Control 115, no. : 107276.
A study on the effects of extruded sorghum bran (ESB) or raw sorghum bran (RSB) on biomarkers of glucose dysregulation, dyslipidemia, inflammation, and antioxidant potential induced by a high-fat diet (HFD) in rats was carried out. Four groups of male Wistar rats (six per group) consumed a basal diet (BD), which were supplemented with either lard at 310 g/kg (HFD) or lard and ESB at 180 g/kg (HFD + ESB) or lard and RSB (HFD + RSB), for 8 weeks. The intake of HFD supplemented with ESB or RSB hindered the fat storage in adipocytes of abdominal tissue, dyslipidemia and the loss of glucose homeostasis. A positive correlation between inflammation biomarkers (Interleukin-1β and Interleukin-6) and glucose homeostasis was observed, while a negative correlation between antioxidant capacity (TEAC) and Interleukin-1β and Interleukin-6 was observed. As a conclusion, the ESB or RSB may be intended as part of healthy diets.
Norma Julieta Salazar-López; Gustavo A. González-Aguilar; Ofelia Rouzaud-Sández; Guadalupe Loarca-Piña; Shela Gorinstein; Maribel Robles-Sánchez. Sorghum bran supplementation ameliorates dyslipidemia, glucose dysregulation, inflammation and stress oxidative induced by a high-fat diet in rats. CyTA - Journal of Food 2019, 18, 20 -30.
AMA StyleNorma Julieta Salazar-López, Gustavo A. González-Aguilar, Ofelia Rouzaud-Sández, Guadalupe Loarca-Piña, Shela Gorinstein, Maribel Robles-Sánchez. Sorghum bran supplementation ameliorates dyslipidemia, glucose dysregulation, inflammation and stress oxidative induced by a high-fat diet in rats. CyTA - Journal of Food. 2019; 18 (1):20-30.
Chicago/Turabian StyleNorma Julieta Salazar-López; Gustavo A. González-Aguilar; Ofelia Rouzaud-Sández; Guadalupe Loarca-Piña; Shela Gorinstein; Maribel Robles-Sánchez. 2019. "Sorghum bran supplementation ameliorates dyslipidemia, glucose dysregulation, inflammation and stress oxidative induced by a high-fat diet in rats." CyTA - Journal of Food 18, no. 1: 20-30.
The properties of durian fruit at five stages of ripeness were evaluated and compared. The physicochemical parameters such as titratable acidity (TA) and total soluble solids (TSS) increased, whereas the pH slightly decreased during the ripening process. The highest contents of polyphenols, flavonoids, flavanols, tannins, vitamin C and the antioxidant capacities, measured by radical scavenging assays, were found in ripe and overripe fruits. The structural properties of extracted polyphenols were evaluated by Fourier transform infrared spectroscopy (FTIR) and fluorescence spectroscopy. The interaction of polyphenols with the main drug carrier in blood human serum albumin (HSA) showed decrease in its fluorescence intensity. The binding properties of polyphenols were in direct correlation with the antioxidant capacities of the investigated fruits. HepG2 cells evaluated cytotoxic effect and the mechanism of cell death after treatment with durian. The metabolism of carbohydrates was examined on the expression of glycolysis-related genes (hexokinase 2 (HK2); 6-phosphofructo-2-kinase 4 (PFKFB4); facilitated glucose transporter member 1 (SLC2A1 (Glut1)) and lactate dehydrogenase A and utilization of glucose in the hepatocytes with durian treatment. Durian in immature stage had stronger cytotoxic effect and weak proapoptotic potential on HepG2 cells than the mature and overripe ones. The ripe and overripe fruits increased the expression of hepatic HK2 and PFKFB4 glycolytic genes and stimulated glucose utilization in HepG2 cells. The present results indicate that durians reveal different biological activity and may provide their broad and extensive use as medicinal or functional foods.
Paweł Paśko; Małgorzata Tyszka-Czochara; Sonia Trojan; Sylwia Bobis-Wozowicz; Paweł Zagrodzki; Jacek Namieśnik; Ratiporn Haruenkit; Sumitra Poovarodom; Praphan Pinsirodom; Shela Gorinstein. Glycolytic genes expression, proapoptotic potential in relation to the total content of bioactive compounds in durian fruits. Food Research International 2019, 125, 108563 .
AMA StylePaweł Paśko, Małgorzata Tyszka-Czochara, Sonia Trojan, Sylwia Bobis-Wozowicz, Paweł Zagrodzki, Jacek Namieśnik, Ratiporn Haruenkit, Sumitra Poovarodom, Praphan Pinsirodom, Shela Gorinstein. Glycolytic genes expression, proapoptotic potential in relation to the total content of bioactive compounds in durian fruits. Food Research International. 2019; 125 ():108563.
Chicago/Turabian StylePaweł Paśko; Małgorzata Tyszka-Czochara; Sonia Trojan; Sylwia Bobis-Wozowicz; Paweł Zagrodzki; Jacek Namieśnik; Ratiporn Haruenkit; Sumitra Poovarodom; Praphan Pinsirodom; Shela Gorinstein. 2019. "Glycolytic genes expression, proapoptotic potential in relation to the total content of bioactive compounds in durian fruits." Food Research International 125, no. : 108563.
This study investigated the effects of drought stress on Chinese cabbage (Chcab) by measuring plant growth responses, total antioxidant enzyme activities, the contents of bioactive compounds including glucosinolates (GLS, aliphatic and indolic), and binding with human serum albumin (HSA). Forty-day-old Chinese cabbage (Brassica rapa L. ssp. pekinensis) seedlings were transplanted into pots and maintained for three weeks at 10% (drought-treated, D-T) and 30% (control, C) soil water. The total leaf number, leaf area, and fresh and dry weights were significantly lower in D-T Chcab than in controls. Total GLSs and catalase activities were found to be significantly higher in D-T Chcab than in controls. Indolic GLSs were significantly higher than aliphatic GLSs in D-T Chcab. These results show that D-T Chcab reduced growth parameters and binding properties with HSA and influenced total contents of GLSs, polyphenols, flavonoids, total antioxidant enzyme activities, catalase and peroxidase.
Rayhan Ahmed Shawon; Baek Song Kang; Sang Gyu Lee; Sung Kyeom Kim; Hee Ju Lee; Elena Katrich; Shela Gorinstein; Yang Gyu Ku. Influence of drought stress on bioactive compounds, antioxidant enzymes and glucosinolate contents of Chinese cabbage (Brassica rapa). Food Chemistry 2019, 308, 125657 .
AMA StyleRayhan Ahmed Shawon, Baek Song Kang, Sang Gyu Lee, Sung Kyeom Kim, Hee Ju Lee, Elena Katrich, Shela Gorinstein, Yang Gyu Ku. Influence of drought stress on bioactive compounds, antioxidant enzymes and glucosinolate contents of Chinese cabbage (Brassica rapa). Food Chemistry. 2019; 308 ():125657.
Chicago/Turabian StyleRayhan Ahmed Shawon; Baek Song Kang; Sang Gyu Lee; Sung Kyeom Kim; Hee Ju Lee; Elena Katrich; Shela Gorinstein; Yang Gyu Ku. 2019. "Influence of drought stress on bioactive compounds, antioxidant enzymes and glucosinolate contents of Chinese cabbage (Brassica rapa)." Food Chemistry 308, no. : 125657.
In the present study evaluation of structural, thermal and antifungal properties of Amaranthus hypochondriacus laboratory protein isolate (ALMA) and commercially available Amaranthus protein dietary antidepressant (APGM) was done by differential scanning calorimetry (DSC), Fourier Transform Infrared (FTIR) and fluorescence spectroscopy and antibiofilm activities against Candida albicans. The results exhibited thermal stability and antioxidant activity for the isolates. Fluorescence measurements showed that they bind to human serum albumin through a static quenching mechanism, decreasing its fluorescence intensity. FTIR spectra showed amides I, II and III shifts, but it does not modify the structural and bioactive properties against C. albicans despite of its infections which is difficult to treat due to virulence expression and biofilm formation that protects of therapeutic drugs. Both isolates had the potential to assuage two virulence factors such as biofilm formation and yeast to hyphal transition of C. albicans. The biofilm inhibitory concentration of the protein isolates was determined to 10 and 30 μg mL−1 with 50% inhibition, while morphogenic transition of the yeast leads to host tissue damage was significantly inhibited in spider medium and in vivo assay with zebrafish embryo. Inhibition of C. albicans biofilm by protein isolates was well compared with COMSTAT and XTT assay. The conformational changes in the proteins of investigated samples were determined by fluorescence after denaturation with 8 M urea and showed slight differences in comparison with the natural product. This is the first study to envisage the use of amaranth protein isolates to immunocompromised patients in their diet plan that can prevent C. albicans infections and help them in recovery. These isolates can be used as natural polymers in biomedical applications and edible films for health benefits.
Rajamohamed Beema Shafreen; Siddharthan Seema; Alma Leticia Martinez-Ayala; María A Lozano-Grande; Maribel Robles-Sánchez; Arkadiusz Szterk; Michael Grishko; Ezra Hanuka; Elena Katrich; Shela Gorinstein. Binding and potential antibiofilm activities of Amaranthus proteins against Candida albicans. Colloids and Surfaces B: Biointerfaces 2019, 183, 110479 .
AMA StyleRajamohamed Beema Shafreen, Siddharthan Seema, Alma Leticia Martinez-Ayala, María A Lozano-Grande, Maribel Robles-Sánchez, Arkadiusz Szterk, Michael Grishko, Ezra Hanuka, Elena Katrich, Shela Gorinstein. Binding and potential antibiofilm activities of Amaranthus proteins against Candida albicans. Colloids and Surfaces B: Biointerfaces. 2019; 183 ():110479.
Chicago/Turabian StyleRajamohamed Beema Shafreen; Siddharthan Seema; Alma Leticia Martinez-Ayala; María A Lozano-Grande; Maribel Robles-Sánchez; Arkadiusz Szterk; Michael Grishko; Ezra Hanuka; Elena Katrich; Shela Gorinstein. 2019. "Binding and potential antibiofilm activities of Amaranthus proteins against Candida albicans." Colloids and Surfaces B: Biointerfaces 183, no. : 110479.
This work evaluated residues of garlic crops from Colombia, as promising sources of cellulosic materials. We demonstrated an efficient re-engineering of the waste products to highly valuable cellulose materials as precursors of cellulosic derivatives suitable for functionalization. To study the feasibility of extracting cellulose from garlic raw fibers, the steam explosion technique was used along with mild chemical treatment. These processes included usual chemical procedures such as alkaline extraction, bleaching, and acid hydrolysis but with a mild concentration of the chemicals. The chemical constituents of the fiber in each processing step were determined by standard procedures. The extracted cellulose microfibers were characterized by Fourier Transform Infrared spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Powder X-ray Diffractometry (XRD), and Scanning Electron Microscope (SEM). The FTIR spectra, as well as XRD, TGA and SEM results, showed changes in the peaks corresponding to hemicelluloses and lignin, which were removed from the fiber surface because of the treatments. These promising results proved revalorization of garlic by-product to produce cellulose derivatives and its potential use as reinforcement in the preparation of useful composites.
Lucia M. Moreno; Shela Gorinstein; Oscar J. Medina; Joaquin Palacios; Efrén J. Muñoz. Valorization of Garlic Crops Residues as Precursors of Cellulosic Materials. Waste and Biomass Valorization 2019, 11, 4767 -4779.
AMA StyleLucia M. Moreno, Shela Gorinstein, Oscar J. Medina, Joaquin Palacios, Efrén J. Muñoz. Valorization of Garlic Crops Residues as Precursors of Cellulosic Materials. Waste and Biomass Valorization. 2019; 11 (9):4767-4779.
Chicago/Turabian StyleLucia M. Moreno; Shela Gorinstein; Oscar J. Medina; Joaquin Palacios; Efrén J. Muñoz. 2019. "Valorization of Garlic Crops Residues as Precursors of Cellulosic Materials." Waste and Biomass Valorization 11, no. 9: 4767-4779.
Persimmon (Diospyros kaki L.) contains a number of metabolites, especially polyphenols and carotenoids, and persimmon is one of the most healthy fruits linking to a diet with reduced incidences of several diseases. However, little data are available on the persimmon in connection with its metabolites and properties. In recent study the differences between polar and non-polar extracts by 1H NMR-based metabolomics was done. The present work presents a complete qualitative analytical characterization by UPLC-ESI-Orbitrap-MS/MS analysis and fluorescence studies in six persimmon cultivars. The metabolic differences were shown as well in the results of bioactivities and antioxidant capacities. The results of binding properties of persimmon polyphenols towards human serum albumin support polyphenol-albumin interactions with human plasma and characteristic to other plants. The presented methods can be widely used for quantitation of multiple compounds in many plants and biological samples, especially in vegetables and fruits.
M. Maulidiani; Nur Ashikin Abdul-Hamid; Faridah Abas; Yong Seo Park; Yang-Kyun Park; Young Mo Kim; Shela Gorinstein. Detection of bioactive compounds in persimmon (Diospyros kaki) using UPLC-ESI-Orbitrap-MS/MS and fluorescence analyses. Microchemical Journal 2019, 149, 103978 .
AMA StyleM. Maulidiani, Nur Ashikin Abdul-Hamid, Faridah Abas, Yong Seo Park, Yang-Kyun Park, Young Mo Kim, Shela Gorinstein. Detection of bioactive compounds in persimmon (Diospyros kaki) using UPLC-ESI-Orbitrap-MS/MS and fluorescence analyses. Microchemical Journal. 2019; 149 ():103978.
Chicago/Turabian StyleM. Maulidiani; Nur Ashikin Abdul-Hamid; Faridah Abas; Yong Seo Park; Yang-Kyun Park; Young Mo Kim; Shela Gorinstein. 2019. "Detection of bioactive compounds in persimmon (Diospyros kaki) using UPLC-ESI-Orbitrap-MS/MS and fluorescence analyses." Microchemical Journal 149, no. : 103978.
The effects of drought stress in autumn-growing Chinese cabbage cultivar on bioactive compounds, total antioxidant and binding properties to human serum albumin (HSA) were studied. Seedlings of 42 days growing Chinese cabbage Brassica rapa L. ssp. pekinensis cv. Bulam Plus (BRARP) were transplanted and maintained into pots during 3 weeks with soil water of the drought-treated (D-T) and control (C) plants at 10% and 30%, respectively. Polyphenols and the values of antioxidant capacities by three radical scavenging assays (CUPRAC, FRAP, ABTS) were determined in methanol extracts of the samples. MS spectra of phenolic extracts were compared in samples treated at different periods of time. The total polyphenols and their activities were slightly lower in drought-treated Chinese cabbage than in corresponding controls. The influence of polyphenols, total antioxidant and binding activities in D-T plants accumulation at drought stress in Chinese cabbage BRARP was reduced. Our study provides valuable information that Chinese cabbage BRARP can be consumed throughout the year in South Korea and other countries.
Yang Gyu Ku; Ho Cheol Kim; Jong Hyang Bae; Baek Song Kang; Alina Nemirovski; Dinorah Barasch; Shela Gorinstein. Antioxidant capacities and polyphenols in autumn-growing cultivar of Chinese cabbage (Brassica rapa L. ssp. pekinensis cv. Bulam Plus). European Food Research and Technology 2019, 245, 1871 -1879.
AMA StyleYang Gyu Ku, Ho Cheol Kim, Jong Hyang Bae, Baek Song Kang, Alina Nemirovski, Dinorah Barasch, Shela Gorinstein. Antioxidant capacities and polyphenols in autumn-growing cultivar of Chinese cabbage (Brassica rapa L. ssp. pekinensis cv. Bulam Plus). European Food Research and Technology. 2019; 245 (9):1871-1879.
Chicago/Turabian StyleYang Gyu Ku; Ho Cheol Kim; Jong Hyang Bae; Baek Song Kang; Alina Nemirovski; Dinorah Barasch; Shela Gorinstein. 2019. "Antioxidant capacities and polyphenols in autumn-growing cultivar of Chinese cabbage (Brassica rapa L. ssp. pekinensis cv. Bulam Plus)." European Food Research and Technology 245, no. 9: 1871-1879.
Methanol extracts of gluten-free pseudocereals (seeds of some Amaranthus species, Chenopodium quinoa and Fagopyrum esculentum) and their by-products (flour, popping, flakes and roasted seeds) were evaluated by Folin–Ciocalteau method, mass spectrometry and fluorescence analysis in order to determine total phenolic content. The values obtained by ABTS, FRAP and CUPRAC radical scavenging assays were used to determine antioxidant capacities. The binding properties of polyphenols were evaluated by their interactions with human serum albumin (HSA). Additionally, in this study were compared selected pseudocereal seeds and by-products in terms of their cytotoxic activity against human cervical carcinoma cell lines (C4-I, HTB-35, HTB-34). The obtained results showed high phenolic contents and their antioxidant, cytotoxic and binding activities in buckwheat following by quinoa and amaranth. The investigated pseudocereals could be used as additives to increase the bioactivity and health properties in foods.
Pawel Pasko; Malgorzata Tyszka-Czochara; Jacek Namieśnik; Zenon Jastrzębski; Hanna Leontowicz; Jerzy Drzewiecki; Alma Leticia Martinez-Ayala; Alina Nemirovski; Dinorah Barasch; Shela Gorinstein. Cytotoxic, antioxidant and binding properties of polyphenols from the selected gluten-free pseudocereals and their by-products: In vitro model. Journal of Cereal Science 2019, 87, 325 -333.
AMA StylePawel Pasko, Malgorzata Tyszka-Czochara, Jacek Namieśnik, Zenon Jastrzębski, Hanna Leontowicz, Jerzy Drzewiecki, Alma Leticia Martinez-Ayala, Alina Nemirovski, Dinorah Barasch, Shela Gorinstein. Cytotoxic, antioxidant and binding properties of polyphenols from the selected gluten-free pseudocereals and their by-products: In vitro model. Journal of Cereal Science. 2019; 87 ():325-333.
Chicago/Turabian StylePawel Pasko; Malgorzata Tyszka-Czochara; Jacek Namieśnik; Zenon Jastrzębski; Hanna Leontowicz; Jerzy Drzewiecki; Alma Leticia Martinez-Ayala; Alina Nemirovski; Dinorah Barasch; Shela Gorinstein. 2019. "Cytotoxic, antioxidant and binding properties of polyphenols from the selected gluten-free pseudocereals and their by-products: In vitro model." Journal of Cereal Science 87, no. : 325-333.
Steam cooking is one of the most common preparations of Momordica charantia dishes. This method improved the tastiness of the fruits but at the same time, some changes occurred in the volatile and non-volatile parts of their food matrices. In this study, for the first time these properties were correlated with the found substances affecting the bioactivity of this fruit. Two varieties of Momordica charantia were analysed and compared. It was possible to differentiate both types of fruits using two-dimensional gas chromatography and time-of-flight mass spectrometry (GC×GC-TOF-MS) as well as to assess botanical and geographical origin. In the case of volatiles, 212 chemical compounds were tentatively identified, which can be classified into seven chemical classes, such as aldehydes, alcohols, esters, ketones, terpenes, hydrocarbons. Furthermore, 16 of them were quantified and calculated in terms of OAV and ROC values. Bioactive substances (polyphenols, flavonoids, tannins and flavanols) and the values of antioxidant capacities by four radical scavenging assays (DPPH, CUPRAC FRAP, ABTS) were determined and compared in water and methanol extracts of Chinese and Indian varieties. It was proven that steam cooked Chinese variety has greater value due to its flavour than Indian variety and consists more volatile, non-volatile and bioactive constituents with high antioxidant effect. The binding properties of polyphenols to HSA were relatively high in comparison with other plants. A strong positive correlation of binding properties and bioactivity of Momordica charantia was estimated. One of the volatiles, namely citronellol, has key importance in respect of antidiabetic effect of Momordica charantia Chinese variety. This study indicates pro-health preponderance of Chinese variety over Indian variety and confirms that steam cooking is in lines with the canons of safe food preparation.
Martyna Lubinska-Szczygeł; Anna Różańska; Jacek Namieśnik; Tomasz Dymerski; Arkadiusz Szterk; Patraporn Luksirikul; Suchada Vearasilp; Elena Katrich; Shela Gorinstein; Martyna Lubinska-Szczygieł. Influence of steam cooking on pro-health properties of Small and Large variety of Momordica charantia. Food Control 2019, 100, 335 -349.
AMA StyleMartyna Lubinska-Szczygeł, Anna Różańska, Jacek Namieśnik, Tomasz Dymerski, Arkadiusz Szterk, Patraporn Luksirikul, Suchada Vearasilp, Elena Katrich, Shela Gorinstein, Martyna Lubinska-Szczygieł. Influence of steam cooking on pro-health properties of Small and Large variety of Momordica charantia. Food Control. 2019; 100 ():335-349.
Chicago/Turabian StyleMartyna Lubinska-Szczygeł; Anna Różańska; Jacek Namieśnik; Tomasz Dymerski; Arkadiusz Szterk; Patraporn Luksirikul; Suchada Vearasilp; Elena Katrich; Shela Gorinstein; Martyna Lubinska-Szczygieł. 2019. "Influence of steam cooking on pro-health properties of Small and Large variety of Momordica charantia." Food Control 100, no. : 335-349.