(1) Background: Hibernation in pens covered with LFC was associated with high mortality of C. aspersum snails in Romanian snail farms. This three-year study aimed to develop a simple, but effective system for protecting breeders in colder climates. (2) Methods: The first phase investigated the (pre)hibernal burrowing behavior and the overwintering habitat choice. Protective structures based on straw, LFC, and/or HDPE were tested at pilot level (no snails). The most suitable system was applied under farm conditions. (3) Results: Wood and ridge-tile micro shelters were significantly preferred to corrugated iron micro shelters. Burrowing specimens acted as shallow-burrowers, and this behaviorwas significantly more common for looser soils. All pilot systems displayed significantly higher thermal protection efficiency compared to the sole use of LFC. The balance between straw moistening and thermal protection favored using structure soil/LFC/straw/10-cm air cushion/HDPE. Its use yielded significantly higher survival compared to the sole use of LFC. Most hibernating snails clustered together in large groups, attached on the lower surface of micro shelters. Predator occurrence appeared to marginally affect overwintering survival. (4) Conclusions: The “sandwich” system could be an effective solution for overwintering mature C. aspersum snails in colder climates.

Stability in time of major and important objectives is vital and can be achieved by 3D scanners which follow changes in time with construction, respective of the natural or artificial hydrotechnical dams and the obtaining of 3D data in real time with the possibility of evaluating and making quick decisions. This scientific paper approaches a research topic of great importance and actuality in the field of Civil Engineering, Hydrotechnics, and Geomatics using the 3D scanning technologies for the hydrotechnical arrangements (Topolovăţu Mic, Coșteiu and Sânmartinu Maghiar) and hydroameliorative (Cruceni Pumping Station). In Romania, data collection was carried out for the first time using the mobile scanning technology (MMS), “Backpack” type, namely, Leica Pegasus Backpack. Data collection using terrestrial laser scanning technology (Terrestrial Laser Scanning) was carried out with the Leica C10 equipment. The processing of point clouds was carried out using the Inertial Explorer program, and the processing of point clouds was carried out with the Cyclone program. The collection of ground checkpoints used for checking, correcting, and analyzing point clouds was carried out using the GPS Leica GS08 equipment. Compared with traditional methods using classical measuring instruments, precise data was obtained (with an error of 2–4 cm) through 3D laser scanning technology in a short time and with multiple possibilities of processing and visualizing point clouds.

This study aims to evaluate the in silico genomic characteristics of five species of the genus Planotetraspora: P. kaengkrachanensis, P. mira, P. phitsanulokensis, P. silvatica, and P. thailandica, with a view to their application in therapeutic research. The 16S rRNA comparison indicated that these species were phylogenetically distinct. Pairwise comparisons of digital DNA-DNA hybridization (dDDH) and OrthoANI values between these studied type strains indicated that dDDH values were below 62.5%, while OrthoANI values were lower than 95.3%, suggesting that the five species represent distinct genomospecies. These results were consistent with the phylogenomic study based on core genes and the pangenome analysis of these five species within the genus Planotetraspora. However, the genome annotation showed some differences between these species, such as variations in the number of subsystem category distributions across whole genomes (ranging between 1979 and 2024). Additionally, the number of CAZYme (Carbohydrate-Active enZYme) genes ranged between 298 and 325, highlighting the potential of these bacteria for therapeutic research applications. The in silico physico-chemical characteristics of cellulases from Planotetraspora species were analyzed. Their 3D structure was modeled, refined, and validated. A molecular docking analysis of this cellulase protein structural model was conducted with cellobiose, cellotetraose, laminaribiose, carboxymethyl cellulose, glucose, and xylose ligand. Our study revealed significant interaction between the Planotetraspora cellulase and cellotetraose substrate, evidenced by stable binding energies. This suggests that this bacterial enzyme holds great potential for utilizing cellotetraose as a substrate in various applications. This study enriches our understanding of the potential applications of Planotetraspora species in therapeutic research.