The removal of oil contaminants in marine intertidal sediments under cold climate is an urgent issue. Although the bioavailability of petroleum hydrocarbons decreases at low temperatures, biosurfactants can promote oil biodegradation. In this study, characteristics of biosurfactants produced by cold-adapted oil-degrading bacteria Planococcus sp. XW-1 were studied. Adding the XW-1 biosurfactant could effectively facilitate the solubility of phenanthrene, pyrene, diesel oil, and crude oil. The solubilization was limited by the number of rings and the molecular weight (WSRphenanthrene = 0.0234; WSRpyrene = 0.0165; WSRdiesel oil = 0.0027; WSRcrude oil = 0.0015). Additional biosurfactants significantly washed out crude oil adsorbed to the sand (reduction from 17.1%, 22.7% to 87.9% and 94.28% in 24 h). With the increase in particle size, the removal efficiency increased from 87.9% to 94.28%. After the addition of biosurfactant, the effect of degradation increased by 20% in 20 days. The results suggest that the biosurfactant-producing bacteria Planococcus sp. XW-1 is a promising candidate used in the in situ bioremediation of petroleum-contaminated intertidal sediment.