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ACO is one of the rate-limiting enzymes in the biosynthesis of ethylene, and it plays a critical role in the regulation of plant growth and development. However, the function of ACO genes in cotton is not well studied. In this study, a total of 332 GhACOs, 187 GaACOs, and 181 GrACOs were identified in G. hirsutum, G. arboretum, and G. raimondii, respectively. Gene duplication analysis showed that whole-genome duplication (WGD) and tandem duplication were the major forces driving the generation of cotton ACO genes. In the promoters of GhACOs, there were cis-acting elements responding to stress, phytohormones, light, and circadian factors, indicating the possible involvement of GhACOs in these processes. Expression and co-expression analyses illustrated that most GhACOs were not only widely expressed in various tissues but also coexpressed with other genes in response to salt and drought stress. GhACO106_At overexpression in Arabidopsis promoted flowering and increased salt tolerance. These results provide a comprehensive overview of the ACO genes of cotton and lay the foundation for subsequent functional studies of these genes.
Hengling Wei; Yujun Xue; Pengyun Chen; Pengbo Hao; Fei Wei; Lu Sun; Yonglin Yang. Genome-Wide Identification and Functional Investigation of 1-Aminocyclopropane-1-carboxylic Acid Oxidase (ACO) Genes in Cotton. Plants 2021, 10, 1699 .
AMA StyleHengling Wei, Yujun Xue, Pengyun Chen, Pengbo Hao, Fei Wei, Lu Sun, Yonglin Yang. Genome-Wide Identification and Functional Investigation of 1-Aminocyclopropane-1-carboxylic Acid Oxidase (ACO) Genes in Cotton. Plants. 2021; 10 (8):1699.
Chicago/Turabian StyleHengling Wei; Yujun Xue; Pengyun Chen; Pengbo Hao; Fei Wei; Lu Sun; Yonglin Yang. 2021. "Genome-Wide Identification and Functional Investigation of 1-Aminocyclopropane-1-carboxylic Acid Oxidase (ACO) Genes in Cotton." Plants 10, no. 8: 1699.
Hybrid breakdown (HB) functions as a common reproductive barrier and reduces hybrid fitness in many species, including cotton. However, the related genes and the underlying genetic mechanisms of HB in cotton remain unknown. Here, we found that the photosensitive genetic male sterile (PGMS) line CCRI9106 was a hybrid progeny of G. hirsutum and G. barbadense and probably a product of HB. Fine mapping with F2s (CCRI9106×G. hirsutum/G. barbadense lines) identified a pair of male sterility genes GoFLA19s (encoding fasciclin-like arabinogalactan family protein) located on chromosomes A12 and D12. Crucial variations occurring in the fasciclin-like domain or/and AGP domain were predicted to cause the nonfunctionalization of GbFLA19-D and GhFLA19-A. CRISPR/Cas9-mediated knockout assay confirmed the effects of GhFLA19s on male sterility. Sequence alignment analyses showed that variations in GbFLA19-D and GhFLA19-A likely occurred after the formation of allotetraploid cotton species. GoFLA19s are specifically expressed in anthers and contribute to tapetal development, exine assembly, intine formation and pollen grain maturation. RNA-seq and qRT-PCR analyses illustrated that genes related to these biological processes were significantly downregulated in the mutant. Our research on male sterility genes, GoFLA19s, improves the understanding of the molecular characteristics and evolutionary significance of HB in interspecific hybrid breeding. SUPPORTING Information
Meng Zhang; Hengling Wei; Ji Liu; Yingjie Bian; Qiang Ma; Guangzhi Mao; Hantao Wang; Aimin Wu; Jingjing Zhang; Pengyun Chen; Liang Ma; Xiaokang Fu; Shuxun Yu. Non‐functional GoFLA19s are responsible for the male sterility caused by hybrid breakdown in cotton ( Gossypium spp.). The Plant Journal 2021, 1 .
AMA StyleMeng Zhang, Hengling Wei, Ji Liu, Yingjie Bian, Qiang Ma, Guangzhi Mao, Hantao Wang, Aimin Wu, Jingjing Zhang, Pengyun Chen, Liang Ma, Xiaokang Fu, Shuxun Yu. Non‐functional GoFLA19s are responsible for the male sterility caused by hybrid breakdown in cotton ( Gossypium spp.). The Plant Journal. 2021; ():1.
Chicago/Turabian StyleMeng Zhang; Hengling Wei; Ji Liu; Yingjie Bian; Qiang Ma; Guangzhi Mao; Hantao Wang; Aimin Wu; Jingjing Zhang; Pengyun Chen; Liang Ma; Xiaokang Fu; Shuxun Yu. 2021. "Non‐functional GoFLA19s are responsible for the male sterility caused by hybrid breakdown in cotton ( Gossypium spp.)." The Plant Journal , no. : 1.
Glycerol-3-phosphate acyltransferases (GPATs), critical for multiple biological processes like male fertility, have been extensively characterized. However, their precise functions and underlying regulatory mechanism in cotton anther development are unclear. This research demonstrated the importance of GhGPAT12/25 (a paralogs pair on A12/D12 sub-chromosome of cotton) to regulate the degradation of tapetum, anther cuticle formation, and pollen exine development. GhGPAT12 and GhGPAT25 exhibited specifically detected transcripts in tapetum and pollen exine during the early anther developmental stages. GhGPAT12/25 are sn-2 glycerol-3-phosphate acyltransferases and can transfer the acyl group of palmitoyl-CoA to glycerol-3-phosphate (G3P). CRISPR/Cas9-mediated knockout identified the functional redundancy of GhGPAT12 and GhGPAT25. Knockout of both genes caused completely male sterility associated with abnormal anther cuticle, swollen tapetum, and inviable microspores with defective exine and irregular unrestricted shape. RNA-seq analysis showed that the loss of function of GhGPAT12/25 affects the processes of wax metabolic, glycerol monomer biosynthesis, and transport. Consistently, cuticular waxes were dramatically reduced in mutant anthers. Yeast one-hybrid system (Y1H), virus-induced gene silencing (VIGS), and dual-luciferase (LUC) assays illustrated that GhMYB80s are likely to directly activate the expression of GhGPAT12/25. This study provides important insights for revealing the regulatory mechanism underlying anther development in cotton.
Meng Zhang; Hengling Wei; Pengbo Hao; Aimin Wu; Qiang Ma; Jingjing Zhang; Hantao Wang; Xiaokang Fu; Liang Ma; Jianhua Lu; Shuxun Yu. GhGPAT12/25 Are Essential for the Formation of Anther Cuticle and Pollen Exine in Cotton (Gossypium hirsutum L.). Frontiers in Plant Science 2021, 12, 1 .
AMA StyleMeng Zhang, Hengling Wei, Pengbo Hao, Aimin Wu, Qiang Ma, Jingjing Zhang, Hantao Wang, Xiaokang Fu, Liang Ma, Jianhua Lu, Shuxun Yu. GhGPAT12/25 Are Essential for the Formation of Anther Cuticle and Pollen Exine in Cotton (Gossypium hirsutum L.). Frontiers in Plant Science. 2021; 12 ():1.
Chicago/Turabian StyleMeng Zhang; Hengling Wei; Pengbo Hao; Aimin Wu; Qiang Ma; Jingjing Zhang; Hantao Wang; Xiaokang Fu; Liang Ma; Jianhua Lu; Shuxun Yu. 2021. "GhGPAT12/25 Are Essential for the Formation of Anther Cuticle and Pollen Exine in Cotton (Gossypium hirsutum L.)." Frontiers in Plant Science 12, no. : 1.
Lint percentage is a major yield component in cotton breeding programmes. To identify quantitative trait loci (QTLs) and candidate genes related to lint percentage, we used an intraspecific recombinant inbred line population of 137 lines derived from Gossypium hirsutum cv. CCRI36 and G. hirsutum acc. G2005 for QTL mapping of lint percentage. Based on a high-density genetic map and phenotype data collected in four growing environments, we identified a total of 28 QTLs for lint percentage. Three stable QTLs (qLP-At5-2, qLP-Dt7-1 and qLP-Dt7-2) were detected in at least two environments. Two genes (Gh_A05G1584 and Gh_A05G1689) containing nonsynonymous single nucleotide polymorphisms (SNPs) were identified by association analysis using published data. The quantitative real-time PCR results showed that the expression levels of Gh_A05G1584 were higher in cv. CCRI36 than in acc. G2005 during all fibre development stages; Gh_A05G1689 was mainly expressed in 15 and 25 days post-anthesis fibres and its expression level was higher in cv. CCRI36. These results suggest candidate genes for lint percentage and provide molecular information for use in cotton breeding programmes aimed at improving yield.
Hantao Wang; Xiaoyun Jia; Meng Kang; Wei Li; Xiaokang Fu; Liang Ma; Jianhua Lu; Hengling Wei; Shuxun Yu. QTL mapping and candidate gene identification of lint percentage based on a recombinant inbred line population of upland cotton. Euphytica 2021, 217, 1 -12.
AMA StyleHantao Wang, Xiaoyun Jia, Meng Kang, Wei Li, Xiaokang Fu, Liang Ma, Jianhua Lu, Hengling Wei, Shuxun Yu. QTL mapping and candidate gene identification of lint percentage based on a recombinant inbred line population of upland cotton. Euphytica. 2021; 217 (6):1-12.
Chicago/Turabian StyleHantao Wang; Xiaoyun Jia; Meng Kang; Wei Li; Xiaokang Fu; Liang Ma; Jianhua Lu; Hengling Wei; Shuxun Yu. 2021. "QTL mapping and candidate gene identification of lint percentage based on a recombinant inbred line population of upland cotton." Euphytica 217, no. 6: 1-12.
Background Male sterility is a simple and efficient pollination control system that is widely exploited in hybrid breeding. In upland cotton, CCRI9106, a photosensitive genetic male sterile (PGMS) mutant isolated from CCRI040029, was reported of great advantages to cotton heterosis. However, little information concerning the male sterility of CCRI9106 is known. Here, comparative transcriptome analysis of CCRI9106 (the mutant, MT) and CCRI040029 (the wild type, WT) anthers in Anyang (long-day, male sterile condition to CCRI9106) was performed to reveal the potential male sterile mechanism of CCRI9106. Results Light and electron microscopy revealed that the male sterility phenotype of MT was mainly attributed to irregularly exine, lacking tryphine and immature anther cuticle. Based on the cytological characteristics of MT anthers, anther RNA libraries (18 in total) of tetrad (TTP), late uninucleate (lUNP) and binucleate (BNP) stages in MT and WT were constructed for transcriptomic analysis, therefore revealing a total of 870,4 differentially expressed genes (DEGs). By performing gene expression pattern analysis and protein-protein interaction (PPI) networks construction, we found down-regulation of DEGs, which enriched by the lipid biosynthetic process and the synthesis pathways of several types of secondary metabolites such as terpenoids, flavonoids and steroids, may crucial to the male sterility phenotype of MT, and resulting in the defects of anther cuticle and tryphine, even the irregularly exine. Furthermore, several lipid-related genes together with ABA-related genes and MYB transcription factors were identified as hub genes via weighted gene co-expression network analysis (WGCNA). Additionally, the ABA content of MT anthers was reduced across all stages when compared with WT anthers. At last, genes related to the formation of anther cuticle and tryphine could activated in MT under short-day condition. Conclusions We propose that the down-regulation of genes related to the assembly of anther cuticle and tryphine may lead to the male sterile phenotype of MT, and MYB transcription factors together with ABA played key regulatory roles in these processes. The conversion of fertility in different photoperiods may closely relate to the functional expression of these genes. These findings contribute to elucidate the mechanism of male sterility in upland cotton.
Meng Zhang; Ji Liu; Qiang Ma; Yuan Qin; Hantao Wang; Pengyun Chen; Liang Ma; Xiaokang Fu; Longfu Zhu; Hengling Wei; Shuxun Yu. Deficiencies in the formation and regulation of anther cuticle and tryphine contribute to male sterility in cotton PGMS line. BMC Genomics 2020, 21, 1 -18.
AMA StyleMeng Zhang, Ji Liu, Qiang Ma, Yuan Qin, Hantao Wang, Pengyun Chen, Liang Ma, Xiaokang Fu, Longfu Zhu, Hengling Wei, Shuxun Yu. Deficiencies in the formation and regulation of anther cuticle and tryphine contribute to male sterility in cotton PGMS line. BMC Genomics. 2020; 21 (1):1-18.
Chicago/Turabian StyleMeng Zhang; Ji Liu; Qiang Ma; Yuan Qin; Hantao Wang; Pengyun Chen; Liang Ma; Xiaokang Fu; Longfu Zhu; Hengling Wei; Shuxun Yu. 2020. "Deficiencies in the formation and regulation of anther cuticle and tryphine contribute to male sterility in cotton PGMS line." BMC Genomics 21, no. 1: 1-18.
Background Valine-glutamine (VQ) motif-containing proteins play important roles in plant growth, development and abiotic stress response. For many plant species, the VQ genes have been identified and their functions have been described. However, little is known about the origin, evolution, and functions (and underlying mechanisms) of the VQ family genes in cotton. Results In this study, we comprehensively analyzed the characteristics of 268 VQ genes from four Gossypium genomes and found that the VQ proteins evolved into 10 clades, and each clade had a similar structural and conservative motif. The expansion of the VQ gene was mainly through segmental duplication, followed by dispersal. Expression analysis revealed that many GhVQs might play important roles in response to salt and drought stress, and GhVQ18 and GhVQ84 were highly expressed under PEG and salt stress. Further analysis showed that GhVQs were co-expressed with GhWRKY transcription factors (TFs), and microRNAs (miRNAs) could hybridize to their cis-regulatory elements. Conclusions The results in this study broaden our understanding of the VQ gene family in plants, and the analysis of the structure, conserved elements, and expression patterns of the VQs provide a solid foundation for exploring their specific functions in cotton responding to abiotic stresses. Our study provides significant insight into the potential functions of VQ genes in cotton.
Pengyun Chen; Fei Wei; Shuaishuai Cheng; Liang Ma; Hantao Wang; Meng Zhang; Guangzhi Mao; Jianhua Lu; Pengbo Hao; Adeel Ahmad; Lijiao Gu; Qiang Ma; Aimin Wu; Hengling Wei; Shuxun Yu. A comprehensive analysis of cotton VQ gene superfamily reveals their potential and extensive roles in regulating cotton abiotic stress. BMC Genomics 2020, 21, 1 -16.
AMA StylePengyun Chen, Fei Wei, Shuaishuai Cheng, Liang Ma, Hantao Wang, Meng Zhang, Guangzhi Mao, Jianhua Lu, Pengbo Hao, Adeel Ahmad, Lijiao Gu, Qiang Ma, Aimin Wu, Hengling Wei, Shuxun Yu. A comprehensive analysis of cotton VQ gene superfamily reveals their potential and extensive roles in regulating cotton abiotic stress. BMC Genomics. 2020; 21 (1):1-16.
Chicago/Turabian StylePengyun Chen; Fei Wei; Shuaishuai Cheng; Liang Ma; Hantao Wang; Meng Zhang; Guangzhi Mao; Jianhua Lu; Pengbo Hao; Adeel Ahmad; Lijiao Gu; Qiang Ma; Aimin Wu; Hengling Wei; Shuxun Yu. 2020. "A comprehensive analysis of cotton VQ gene superfamily reveals their potential and extensive roles in regulating cotton abiotic stress." BMC Genomics 21, no. 1: 1-16.
MADS-box gene family plays an important role in the molecular regulatory network of flower development. APETALA1 (AP1), a MADS-box gene, plays an important role in the development of flower organs. Although many studies about MADS-box family genes have been reported, the function of AP1 is still not clear in cotton. In this study, GhAP1.7 (Gh_D03G0922), a candidate gene for cotton flower time and plant height obtained from our previous studies, was cloned from CCRI50 cotton variety and functionally characterized. Subcellular localization demonstrated that GhAP1.7 was located in nucleus. Infection test of Arabidopsis revealed that GhAP1.7 could cause precocious flowering and virus-induced gene silence (VIGS) assay demonstrated that GhAP1.7 could lead to delayed flowering of cotton plants. Yeast one-hybrid assays and transient dual-luciferase assays suggested that floral meristem identity control gene LEAFY (LFY) can bind the promoter of GhAP1.7 and negatively regulate it. Our research indicated that GhAP1.7 might work as a positive regulator in plant flowering. Moreover, GhAP1.7 may negatively regulated by GhLFY in the regulatory pathways. This work laid the foundation for subsequent functional studies of GhAP1.7.
Xiaoqian Cheng; Hantao Wang; Hengling Wei; Lijiao Gu; Pengbo Hao; Huiru Sun; Aimin Wu; Shuaishuai Cheng; Shuxun Yu. The MADS transcription factor GhAP1.7 coordinates the flowering regulatory pathway in upland cotton (Gossypium hirsutum L.). Gene 2020, 769, 145235 .
AMA StyleXiaoqian Cheng, Hantao Wang, Hengling Wei, Lijiao Gu, Pengbo Hao, Huiru Sun, Aimin Wu, Shuaishuai Cheng, Shuxun Yu. The MADS transcription factor GhAP1.7 coordinates the flowering regulatory pathway in upland cotton (Gossypium hirsutum L.). Gene. 2020; 769 ():145235.
Chicago/Turabian StyleXiaoqian Cheng; Hantao Wang; Hengling Wei; Lijiao Gu; Pengbo Hao; Huiru Sun; Aimin Wu; Shuaishuai Cheng; Shuxun Yu. 2020. "The MADS transcription factor GhAP1.7 coordinates the flowering regulatory pathway in upland cotton (Gossypium hirsutum L.)." Gene 769, no. : 145235.
Gossypium barbadense is an important source of natural textile fibers, as is Gossypium hirsutum. Cotton fiber development is often affected by various environmental factors, such as abnormal temperature. However, little is known about the underlying mechanisms of temperature regulating the fuzz fiber initiation. In this study, we reveal that high temperatures (HT) accelerate fiber development, improve fiber quality, and induced fuzz initiation of a thermo-sensitive G. barbadense variety L7009. It was proved that fuzz initiation was inhibited by low temperature (LT), and 4 dpa was the stage most susceptible to temperature stress during the fuzz initiation period. A total of 43,826 differentially expressed genes (DEGs) were identified through comparative transcriptome analysis. Of these, 9667 were involved in fiber development and temperature response with 901 transcription factor genes and 189 genes related to plant hormone signal transduction. Further analysis of gene expression patterns revealed that 240 genes were potentially involved in fuzz initiation induced by high temperature. Functional annotation revealed that the candidate genes related to fuzz initiation were significantly involved in the asparagine biosynthetic process, cell wall biosynthesis, and stress response. The expression trends of sixteen genes randomly selected from the RNA-seq data were almost consistent with the results of qRT-PCR. Our study revealed several potential candidate genes and pathways related to fuzz initiation induced by high temperature. This provides a new view of temperature-induced tissue and organ development in Gossypium barbadense.
Gongmin Cheng; Longyan Zhang; Hengling Wei; Hantao Wang; Jianhua Lu; Shuxun Yu. Transcriptome Analysis Reveals a Gene Expression Pattern Associated with Fuzz Fiber Initiation Induced by High Temperature in Gossypium barbadense. Genes 2020, 11, 1066 .
AMA StyleGongmin Cheng, Longyan Zhang, Hengling Wei, Hantao Wang, Jianhua Lu, Shuxun Yu. Transcriptome Analysis Reveals a Gene Expression Pattern Associated with Fuzz Fiber Initiation Induced by High Temperature in Gossypium barbadense. Genes. 2020; 11 (9):1066.
Chicago/Turabian StyleGongmin Cheng; Longyan Zhang; Hengling Wei; Hantao Wang; Jianhua Lu; Shuxun Yu. 2020. "Transcriptome Analysis Reveals a Gene Expression Pattern Associated with Fuzz Fiber Initiation Induced by High Temperature in Gossypium barbadense." Genes 11, no. 9: 1066.
Young cotyledons of cotton seedlings are most susceptible to chilling stress. To gain insight into the potential mechanism of cold tolerance of young cotton cotyledons, we conducted physiological and comparative transcriptome analysis of two varieties with contrasting phenotypes. The evaluation of chilling injury of young cotyledons among 74 cotton varieties revealed that H559 was the most tolerant and YM21 was the most sensitive. The physiological analysis found that the ROS scavenging ability was lower, and cell membrane damage was more severe in the cotyledons of YM21 than that of H559 under chilling stress. RNA-seq analysis identified a total of 44,998 expressed genes and 19,982 differentially expressed genes (DEGs) in young cotyledons of the two varieties under chilling stress. Weighted gene coexpression network analysis (WGCNA) of all DEGs revealed four significant modules with close correlation with specific samples. The GO-term enrichment analysis found that lots of genes in H559-specific modules were involved in plant resistance to abiotic stress. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that pathways such as plant hormone signal transduction, MAPK signaling, and plant–pathogen interaction were related to chilling stress response. A total of 574 transcription factors and 936 hub genes in these modules were identified. Twenty hub genes were selected for qRT-PCR verification, revealing the reliability and accuracy of transcriptome data. These findings will lay a foundation for future research on the molecular mechanism of cold tolerance in cotyledons of cotton.
Gongmin Cheng; Longyan Zhang; Hantao Wang; Jianhua Lu; Hengling Wei; Shuxun Yu. Transcriptomic Profiling of Young Cotyledons Response to Chilling Stress in Two Contrasting Cotton (Gossypium hirsutum L.) Genotypes at the Seedling Stage. International Journal of Molecular Sciences 2020, 21, 5095 .
AMA StyleGongmin Cheng, Longyan Zhang, Hantao Wang, Jianhua Lu, Hengling Wei, Shuxun Yu. Transcriptomic Profiling of Young Cotyledons Response to Chilling Stress in Two Contrasting Cotton (Gossypium hirsutum L.) Genotypes at the Seedling Stage. International Journal of Molecular Sciences. 2020; 21 (14):5095.
Chicago/Turabian StyleGongmin Cheng; Longyan Zhang; Hantao Wang; Jianhua Lu; Hengling Wei; Shuxun Yu. 2020. "Transcriptomic Profiling of Young Cotyledons Response to Chilling Stress in Two Contrasting Cotton (Gossypium hirsutum L.) Genotypes at the Seedling Stage." International Journal of Molecular Sciences 21, no. 14: 5095.
The transition from vegetative to reproductive growth is very important for early maturity in cotton. However, the genetic control of this highly dynamic and complex developmental process remains unclear. A high‐resolution tissue‐ and stage‐specific transcriptome profile was generated from six developmental stages using 72 samples of two early‐maturing and two late‐maturing cotton varieties. The results of histological analysis of paraffin sections showed that flower bud differentiation occurred at the third true leaf stage (3TLS) in early‐maturing varieties, but at the fifth true leaf stage (5TLS) in late‐maturing varieties. Using pairwise comparison and weighted gene co‐expression network analysis, 5,312 differentially expressed genes were obtained, which were divided into 10 gene co‐expression modules. In the MElightcyan module, 46 candidate genes regulating cotton flower bud differentiation were identified and expressed at the flower bud differentiation stage. A novel key regulatory gene related to flower bud differentiation, GhCAL , was identified in the MElightcyan module. Anti‐GhCAL transgenic cotton plants exhibited late flower bud differentiation and flowering time. GhCAL formed heterodimers with GhAP1‐A04/GhAGL6‐D09 and regulated the expression of GhAP1‐A04 and GhAGL6‐D09 . GhAP1‐A04 and GhAGL6‐D09 silenced plants also showed significant late flowering. Finally, we propose a new flowering regulatory pathway mediated by GhCAL . This study elucidated the molecular mechanism of cotton flowering regulation and provides good genetic resources for cotton early‐maturing breeding.
Shuaishuai Cheng; Pengyun Chen; ZhengZheng Su; Liang Ma; Pengbo Hao; Jingjing Zhang; Qiang Ma; Guoyuan Liu; Ji Liu; Hantao Wang; Hengling Wei; Shuxun Yu. High‐resolution temporal dynamic transcriptome landscape reveals a GhCAL ‐mediated flowering regulatory pathway in cotton ( Gossypium hirsutum L.). Plant Biotechnology Journal 2020, 19, 153 -166.
AMA StyleShuaishuai Cheng, Pengyun Chen, ZhengZheng Su, Liang Ma, Pengbo Hao, Jingjing Zhang, Qiang Ma, Guoyuan Liu, Ji Liu, Hantao Wang, Hengling Wei, Shuxun Yu. High‐resolution temporal dynamic transcriptome landscape reveals a GhCAL ‐mediated flowering regulatory pathway in cotton ( Gossypium hirsutum L.). Plant Biotechnology Journal. 2020; 19 (1):153-166.
Chicago/Turabian StyleShuaishuai Cheng; Pengyun Chen; ZhengZheng Su; Liang Ma; Pengbo Hao; Jingjing Zhang; Qiang Ma; Guoyuan Liu; Ji Liu; Hantao Wang; Hengling Wei; Shuxun Yu. 2020. "High‐resolution temporal dynamic transcriptome landscape reveals a GhCAL ‐mediated flowering regulatory pathway in cotton ( Gossypium hirsutum L.)." Plant Biotechnology Journal 19, no. 1: 153-166.
Background Multiple C2 domains and transmembrane region proteins (MCTPs) may act as transport mediators of other regulators. Although increased number of MCTPs in higher plants implies their diverse and specific functions in plant growth and development, only a few plant MCTPs have been studied and no study on the MCTPs in cotton has been reported. Results In this study, we identified 31 MCTPs in G. hirsutum, which were classified into five subfamilies according to the phylogenetic analysis. GhMCTPs from subfamily V exhibited isoelectric points (pIs) less than 7, whereas GhMCTPs from subfamily I, II, III and IV exhibited pIs more than 7.5, implying their distinct biological functions. In addition, GhMCTPs within subfamily III, IV and V exhibited more diverse physicochemical properties, domain architectures and expression patterns than GhMCTPs within subfamily I and II, suggesting that GhMCTPs within subfamily III, IV and V diverged to perform more diverse and specific functions. Analyses of conserved motifs and pIs indicated that the N-terminus was more divergent than the C-terminus and GhMCTPs’ functional divergence might be mainly contributed by the N-terminus. Furthermore, yeast two-hybrid assay indicated that the N-terminus was responsible to interact with target proteins. Phylogenetic analysis classified multiple N-terminal C2 domains into four subclades, suggesting that these C2 domains performed different molecular functions in mediating the transport of target proteins. Conclusions Our systematic characterization of MCTPs in G. hirsutum will provide helpful information to further research GhMCTPs’ molecular roles in mediating other regulators’ transport to coordinate growth and development of various cotton tissues.
Pengbo Hao; Hantao Wang; Liang Ma; Aimin Wu; Pengyun Chen; Shuaishuai Cheng; Hengling Wei; Shuxun Yu. Genome-wide identification and characterization of multiple C2 domains and transmembrane region proteins in Gossypium hirsutum. BMC Genomics 2020, 21, 1 -16.
AMA StylePengbo Hao, Hantao Wang, Liang Ma, Aimin Wu, Pengyun Chen, Shuaishuai Cheng, Hengling Wei, Shuxun Yu. Genome-wide identification and characterization of multiple C2 domains and transmembrane region proteins in Gossypium hirsutum. BMC Genomics. 2020; 21 (1):1-16.
Chicago/Turabian StylePengbo Hao; Hantao Wang; Liang Ma; Aimin Wu; Pengyun Chen; Shuaishuai Cheng; Hengling Wei; Shuxun Yu. 2020. "Genome-wide identification and characterization of multiple C2 domains and transmembrane region proteins in Gossypium hirsutum." BMC Genomics 21, no. 1: 1-16.
Background: Male sterility is a simple and efficient pollination control system that is widely exploited in hybrid breeding. In upland cotton, CCRI9106, a photosensitive genetic male sterile (PGMS) mutant isolated from CCRI040029, was reported of great advantages to cotton heterosis. However, the underlying molecular mechanism of CCRI9106 remains unclear.Results: In this study, light and electron microscopy revealed that the male sterility phenotype of MT was mainly attributed to irregularly exine, lacking tryphine and immature anther cuticle. Based on the cytological characteristics of MT anthers, 18 RNA libraries were constructed from the anthers of MT and WT at tetrad (TTP), late uninucleate (LUNP) and binucleate (BNP) stages of anther development for transcriptomic analysis, therefore revealing a total of 870,4 differentially expressed genes (DEGs). By performing gene expression pattern analysis and protein-protein interaction (PPI) networks construction, we found down-regulation of DEGs in cluster 2, which enriched by the lipid biosynthetic process and the synthesis pathways of several types of secondary metabolites such as terpenoids, flavonoids and steroids, may crucial to the male sterility phenotype of MT, and resulting in the defects of anther cuticle and tryphine, even the irregularly exine. Furthermore, several lipid-related genes together with ABA-related genes and MYB transcription factors were identified as hub genes via weighted gene co-expression network analysis (WGCNA), such as NPC2, LTPG, LTP1, MAKR6, Ghir_D11G032630, Ghir_A01G008890, Ghir_D01G009320, MYB3, MYB7, MYB16 and MYB48. Additionally, the ABA content of MT anthers was reduced across all stage when compared with WT anthers.Conclusions: In summary, we propose that the down-regulation of genes related to the assembly of anther cuticle and tryphine may lead to the male sterile phenotype of MT, and MYB transcription factors together with ABA play key regulatory role in these processes. These findings provide valuable information on the transcriptional level to anther and pollen development, and contribute to elucidate the mechanism of male sterility in upland cotton.
Meng Zhang; Ji Liu; Qiang Ma; Yuan Qin; Hantao Wang; Pengyun Chen; Liang Ma; Xiaokang Fu; Longfu Zhu; Hengling Wei; Shuxun Yu. Deficiencies in the formation and regulation of anther cuticle and tryphine contribute to male sterility in cotton PGMS line. 2020, 1 .
AMA StyleMeng Zhang, Ji Liu, Qiang Ma, Yuan Qin, Hantao Wang, Pengyun Chen, Liang Ma, Xiaokang Fu, Longfu Zhu, Hengling Wei, Shuxun Yu. Deficiencies in the formation and regulation of anther cuticle and tryphine contribute to male sterility in cotton PGMS line. . 2020; ():1.
Chicago/Turabian StyleMeng Zhang; Ji Liu; Qiang Ma; Yuan Qin; Hantao Wang; Pengyun Chen; Liang Ma; Xiaokang Fu; Longfu Zhu; Hengling Wei; Shuxun Yu. 2020. "Deficiencies in the formation and regulation of anther cuticle and tryphine contribute to male sterility in cotton PGMS line." , no. : 1.
Genetic male sterility (GMS) facilitates hybrid seed production in crops including cotton (Gossypium hirsutum). However, the genetic and molecular mechanisms specifically involved in this developmental process are poorly understood. In this study, small RNA sequencing, degradome sequencing, and transcriptome sequencing were performed to analyze miRNAs and their target genes during anther development in a GMS mutant (‘Dong A’) and its fertile wildtype (WT). A total of 80 known and 220 novel miRNAs were identified, 71 of which showed differential expressions during anther development. A further degradome sequencing revealed a total of 117 candidate target genes cleaved by 16 known and 36 novel miRNAs. Based on RNA-seq, 24, 11, and 21 predicted target genes showed expression correlations with the corresponding miRNAs at the meiosis, tetrad and uninucleate stages, respectively. In addition, a large number of differentially expressed genes were identified, most of which were involved in sucrose and starch metabolism, carbohydrate metabolism, and plant hormone signal transduction based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The results of our study provide valuable information for further functional investigations of the important miRNAs and target genes involved in genetic male sterility and advance our understanding of miRNA regulatory functions during cotton anther development.
Dingwei Yu; Libei Li; Hengling Wei; Shuxun Yu. Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing. Molecular Genetics and Genomics 2020, 295, 645 -660.
AMA StyleDingwei Yu, Libei Li, Hengling Wei, Shuxun Yu. Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing. Molecular Genetics and Genomics. 2020; 295 (3):645-660.
Chicago/Turabian StyleDingwei Yu; Libei Li; Hengling Wei; Shuxun Yu. 2020. "Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing." Molecular Genetics and Genomics 295, no. 3: 645-660.
Pectate lyases (PELs) play important roles in plant growth and development, mainly by degrading the pectin in primary cell walls. However, the role of PELs in cotton fiber elongation, which also involves changes in cellular structure and components, is poorly understood. Therefore, we aimed to isolate and characterize GhPEL76, as we suspected it to contribute to the regulation of fiber elongation. Expression analysis (qRT-PCR) revealed that GhPEL76 is predominately expressed in cotton fiber, with significantly different expression levels in long- and short-fiber cultivars, and that GhPEL76 expression is responsive to gibberellic acid and indoleacetic acid treatment. Furthermore, GhPEL76 promoter-driven β-glucuronidase activity was detected in the roots, hypocotyls, and leaves of transgenic Arabidopsis plants, and the overexpression of GhPEL76 in transgenic Arabidopsis promoted the elongation of several organs, including petioles, hypocotyls, primary roots, and trichomes. Additionally, the virus-induced silencing of GhPEL76 in cotton reduced fiber length, and both yeast one-hybrid and transient dual-luciferase assays suggested that GhbHLH13, a bHLH transcription factor that is up-regulated during fiber elongation, activates GhPEL76 expression by binding to the G-box of the GhPEL76 promoter region. Therefore, these results suggest GhPEL76 positively regulates fiber elongation and provide a basis for future studies of cotton fiber development.
Huiru Sun; Pengbo Hao; Lijiao Gu; Shuaishuai Cheng; Hantao Wang; Aimin Wu; Liang Ma; Hengling Wei; Shuxun Yu. Pectate lyase-like Gene GhPEL76 regulates organ elongation in Arabidopsis and fiber elongation in cotton. Plant Science 2019, 293, 110395 .
AMA StyleHuiru Sun, Pengbo Hao, Lijiao Gu, Shuaishuai Cheng, Hantao Wang, Aimin Wu, Liang Ma, Hengling Wei, Shuxun Yu. Pectate lyase-like Gene GhPEL76 regulates organ elongation in Arabidopsis and fiber elongation in cotton. Plant Science. 2019; 293 ():110395.
Chicago/Turabian StyleHuiru Sun; Pengbo Hao; Lijiao Gu; Shuaishuai Cheng; Hantao Wang; Aimin Wu; Liang Ma; Hengling Wei; Shuxun Yu. 2019. "Pectate lyase-like Gene GhPEL76 regulates organ elongation in Arabidopsis and fiber elongation in cotton." Plant Science 293, no. : 110395.
Background Cotton fiber length and strength are both key traits of fiber quality, and fiber strength (FS) is tightly correlated with secondary cell wall (SCW) biosynthesis. The three-amino-acid-loop-extension (TALE) superclass homeoproteins are involved in regulating diverse biological processes in plants, and some TALE members has been identified to play a key role in regulating SCW formation. However, little is known about the functions of TALE members in cotton (Gossypium spp.). Results In the present study, based on gene homology, 46, 47, 88 and 94 TALE superfamily genes were identified in G. arboreum, G. raimondii, G. barbadense and G. hirsutum, respectively. Phylogenetic and evolutionary analysis showed the evolutionary conservation of two cotton TALE families (including BEL1-like and KNOX families). Gene structure analysis also indicated the conservation of GhTALE members under selection. The analysis of promoter cis-elements and expression patterns suggested potential transcriptional regulation functions in fiber SCW biosynthesis and responses to some phytohormones for GhTALE proteins. Genome-wide analysis of colocalization of TALE transcription factors with SCW-related QTLs revealed that some BEL1-like genes and KNAT7 homologs may participate in the regulation of cotton fiber strength formation. Overexpression of GhKNAT7-A03 and GhBLH6-A13 significantly inhibited the synthesis of lignocellulose in interfascicular fibers of Arabidopsis. Yeast two-hybrid (Y2H) experiments showed extensive heteromeric interactions between GhKNAT7 homologs and some GhBEL1-like proteins. Yeast one-hybrid (Y1H) experiments identified the upstream GhMYB46 binding sites in the promoter region of GhTALE members and defined the downstream genes that can be directly bound and regulated by GhTALE heterodimers. Conclusion We comprehensively identified TALE superfamily genes in cotton. Some GhTALE members are predominantly expressed during the cotton fiber SCW thicking stage, and may genetically correlated with the formation of FS. Class II KNOX member GhKNAT7 can interact with some GhBEL1-like members to form the heterodimers to regulate the downstream targets, and this regulatory relationship is partially conserved with Arabidopsis. In summary, this study provides important clues for further elucidating the functions of TALE genes in regulating cotton growth and development, especially in the fiber SCW biosynthesis network, and it also contributes genetic resources to the improvement of cotton fiber quality.
Qiang Ma; Nuohan Wang; Pengbo Hao; Huiru Sun; Congcong Wang; Liang Ma; Hantao Wang; Xianlong Zhang; Hengling Wei; Shuxun Yu. Genome-wide identification and characterization of TALE superfamily genes in cotton reveals their functions in regulating secondary cell wall biosynthesis. BMC Plant Biology 2019, 19, 1 -20.
AMA StyleQiang Ma, Nuohan Wang, Pengbo Hao, Huiru Sun, Congcong Wang, Liang Ma, Hantao Wang, Xianlong Zhang, Hengling Wei, Shuxun Yu. Genome-wide identification and characterization of TALE superfamily genes in cotton reveals their functions in regulating secondary cell wall biosynthesis. BMC Plant Biology. 2019; 19 (1):1-20.
Chicago/Turabian StyleQiang Ma; Nuohan Wang; Pengbo Hao; Huiru Sun; Congcong Wang; Liang Ma; Hantao Wang; Xianlong Zhang; Hengling Wei; Shuxun Yu. 2019. "Genome-wide identification and characterization of TALE superfamily genes in cotton reveals their functions in regulating secondary cell wall biosynthesis." BMC Plant Biology 19, no. 1: 1-20.
Improving the yield and fiber quality of upland cotton is a goal of plant breeders. However, increasing the yield and quality of cotton fibers is becoming more urgent. While the growing human population needs more cotton fiber, climate change is reducing the amount of land on which cotton can be planted, or making it difficult to ensure that water and other resources will be available in optimal quantities. The most logical means of improving yield and quality is understanding and manipulating the genes involved. Here, we used comparative transcriptomics to explore differences in gene expression between long- and short-fiber cotton lines to identify candidate genes useful for cotton improvement. Light and electron microscopy revealed that the initial fiber density was significantly greater in our short-fiber group (SFG) than in our long-fiber group (LFG). Compared with the SFG fibers, the LFG fibers were longer at all developmental stages. Comparison of the LFG and SFG transcriptomes revealed a total of 3538 differentially expressed genes (DEGs). Notably, at all three developmental stages examined, two expression patterns, consistently downregulated (profile 0) and consistently upregulated (profile 7), were identified, and both were significantly enriched in the SFG and LFG. Twenty-two DEGs known to be involved in fiber initiation were detected in profile 0, while 31 DEGs involved in fiber elongation were detected in profile 7. Functional annotation suggested that these DEGs, which included ERF1, TUA2, TUB1, and PER64, affect fiber elongation by participating in the ethylene response, microtubule synthesis, and/or the peroxidase (POD) catalytic pathway. qRT-PCR was used to confirm the RNA sequencing results for select genes. A comparison of SFG and LFG transcription profiles revealed modest but important differences in gene expression between the groups. Notably, our results confirm those of previous studies suggesting that genes involved in ethylene, tubulin, and POD pathways play important roles in fiber development. The 22 consistently downregulated DEGs involved in fiber initiation and the 31 consistently upregulated genes involved in fiber elongation are seemingly good candidate genes for improving fiber initiation and elongation in cotton.
Yuan Qin; Huiru Sun; Pengbo Hao; Hantao Wang; Congcong Wang; Liang Ma; Hengling Wei; Shuxun Yu. Transcriptome analysis reveals differences in the mechanisms of fiber initiation and elongation between long- and short-fiber cotton (Gossypium hirsutum L.) lines. BMC Genomics 2019, 20, 1 -16.
AMA StyleYuan Qin, Huiru Sun, Pengbo Hao, Hantao Wang, Congcong Wang, Liang Ma, Hengling Wei, Shuxun Yu. Transcriptome analysis reveals differences in the mechanisms of fiber initiation and elongation between long- and short-fiber cotton (Gossypium hirsutum L.) lines. BMC Genomics. 2019; 20 (1):1-16.
Chicago/Turabian StyleYuan Qin; Huiru Sun; Pengbo Hao; Hantao Wang; Congcong Wang; Liang Ma; Hengling Wei; Shuxun Yu. 2019. "Transcriptome analysis reveals differences in the mechanisms of fiber initiation and elongation between long- and short-fiber cotton (Gossypium hirsutum L.) lines." BMC Genomics 20, no. 1: 1-16.
Many BURP domain-containing proteins, which are unique to plants, have been identified. They performed diverse functions in plant development and the stress response. To date, only a few BURP domain-containing genes have been studied, and no comprehensive analysis of the gene family in cotton has been reported. In this study, 18, 17 and 30 putative BURP genes were identified in G. raimondii (D5), G. arboreum (A2) and G. hirsutum (AD1), respectively. These BURP genes were phylogenetically classified into eight subfamilies, which were confirmed by analyses of gene structures, motifs and protein domains. The uneven distribution of BURPs in chromosomes and gene duplication analysis indicated that segmental duplication might be the main driving force of the GhBURP family expansion. Promoter regions of all GhBURPs contained at least one putative stress-related cis-elements. Analysis of transcriptomic data and qRT-PCR showed that GhBURPs showed different expression patterns in different organs, and all of them, especially the members of the RD22-like subfamily, could be induced by different stresses, such as abscisic acid (ABA) and salicylic acid (SA), which indicated that the GhBURPs may performed important functions in cotton’s responses to various abiotic stresses. Our study comprehensively analyzed BURP genes in G. hirsutum, providing insight into the functions of GhBURPs in cotton development and adaptation to stresses.
Huiru Sun; Hengling Wei; Hantao Wang; Pengbo Hao; Lijiao Gu; Guoyuan Liu; Liang Ma; ZhengZheng Su; Shuxun Yu. Genome-wide identification and expression analysis of the BURP domain-containing genes in Gossypium hirsutum. BMC Genomics 2019, 20, 1 -19.
AMA StyleHuiru Sun, Hengling Wei, Hantao Wang, Pengbo Hao, Lijiao Gu, Guoyuan Liu, Liang Ma, ZhengZheng Su, Shuxun Yu. Genome-wide identification and expression analysis of the BURP domain-containing genes in Gossypium hirsutum. BMC Genomics. 2019; 20 (1):1-19.
Chicago/Turabian StyleHuiru Sun; Hengling Wei; Hantao Wang; Pengbo Hao; Lijiao Gu; Guoyuan Liu; Liang Ma; ZhengZheng Su; Shuxun Yu. 2019. "Genome-wide identification and expression analysis of the BURP domain-containing genes in Gossypium hirsutum." BMC Genomics 20, no. 1: 1-19.
Premature senescence can reduce the yield and quality of crops. WRKY transcription factors (TFs) play important roles during leaf senescence, but little is known about their ageing mechanisms in cotton. In this study, a group III WRKY TF, GhWRKY27, was isolated and characterized. The expression of GhWRKY27 was induced by leaf senescence and was higher in an early-ageing cotton variety than in a non-early-ageing cotton variety. Overexpression of GhWRKY27 in Arabidopsis promoted leaf senescence, as determined by reduced chlorophyll content and elevated expression of senescence-associated genes (SAGs). Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that GhWRKY27 interacted with an MYB TF, GhTT2. Putative target genes of GhWRKY27 were identified via chromatin immunoprecipitation followed by sequencing (ChIP-seq). Yeast one-hybrid (Y1H) assay and electrophoretic mobility shift assay (EMSA) revealed that GhWRKY27 binds directly to the promoters of cytochrome P450 94C1 (GhCYP94C1) and ripening-related protein 2 (GhRipen2–2). In addition, the expression patterns of GhTT2, GhCYP94C1 and GhRipen2–2 were identified during leaf senescence. Transient dual-luciferase reporter assay indicated that GhWRKY27 could activate the expression of GhCYP94C1 and GhRipen2–2. Our work lays the foundation for further study of the functional roles of WRKY genes during leaf senescence in cotton. In addition, our data provide new insights into the senescence-associated mechanisms of WRKY genes in cotton.
Lijiao Gu; Lingling Dou; Yaning Guo; Hantao Wang; Libei Li; Congcong Wang; Liang Ma; Hengling Wei; Shuxun Yu. The WRKY transcription factor GhWRKY27 coordinates the senescence regulatory pathway in upland cotton (Gossypium hirsutum L.). BMC Plant Biology 2019, 19, 1 -14.
AMA StyleLijiao Gu, Lingling Dou, Yaning Guo, Hantao Wang, Libei Li, Congcong Wang, Liang Ma, Hengling Wei, Shuxun Yu. The WRKY transcription factor GhWRKY27 coordinates the senescence regulatory pathway in upland cotton (Gossypium hirsutum L.). BMC Plant Biology. 2019; 19 (1):1-14.
Chicago/Turabian StyleLijiao Gu; Lingling Dou; Yaning Guo; Hantao Wang; Libei Li; Congcong Wang; Liang Ma; Hengling Wei; Shuxun Yu. 2019. "The WRKY transcription factor GhWRKY27 coordinates the senescence regulatory pathway in upland cotton (Gossypium hirsutum L.)." BMC Plant Biology 19, no. 1: 1-14.
Pectin is a major component and structural polysaccharide of the primary cell walls and middle lamella of higher plants. Pectate lyase (PEL, EC 4.2.2.2), a cell wall modification enzyme, degrades de-esterified pectin for cell wall loosening, remodeling and rearrangement. Nevertheless, there have been few studies on PEL genes and no comprehensive analysis of the PEL gene family in cotton. We identified 53, 42 and 83 putative PEL genes in Gossypium raimondii (D5), Gossypium arboreum (A2), and Gossypium hirsutum (AD1), respectively. These PEL genes were classified into five subfamilies (I-V). Members from the same subfamilies showed relatively conserved gene structures, motifs and protein domains. An analysis of gene chromosomal locations and gene duplication revealed that segmental duplication likely contributed to the expansion of the GhPELs. The 2000 bp upstream sequences of all the GhPELs contained auxin response elements. A transcriptomic data analysis showed that 62 GhPELs were expressed in various tissues. Notably, most (29/32) GhPELs of subfamily IV were preferentially expressed in the stamen, and five GhPELs of subfamily V were prominently expressed at the fiber elongation stage. In addition, qRT-PCR analysis revealed the expression characteristics of 24 GhPELs in four pollen developmental stages and significantly different expression of some GhPELs between long- and short-fiber cultivars. Moreover, some members were responsive to IAA treatment. The results indicate that GhPELs play significant and functionally diverse roles in the development of different tissues. In this study, we comprehensively analyzed PELs in G. hirsutum, providing a foundation to better understand the functions of GhPELs in different tissues and pathways, especially in pollen, fiber and the auxin signaling pathway.
Huiru Sun; Pengbo Hao; Qiang Ma; Meng Zhang; Yuan Qin; Hengling Wei; Junji Su; Hantao Wang; Lijiao Gu; Nuohan Wang; Guoyuan Liu; Shuxun Yu. Genome-wide identification and expression analyses of the pectate lyase (PEL) gene family in cotton (Gossypium hirsutum L.). BMC Genomics 2018, 19, 661 .
AMA StyleHuiru Sun, Pengbo Hao, Qiang Ma, Meng Zhang, Yuan Qin, Hengling Wei, Junji Su, Hantao Wang, Lijiao Gu, Nuohan Wang, Guoyuan Liu, Shuxun Yu. Genome-wide identification and expression analyses of the pectate lyase (PEL) gene family in cotton (Gossypium hirsutum L.). BMC Genomics. 2018; 19 (1):661.
Chicago/Turabian StyleHuiru Sun; Pengbo Hao; Qiang Ma; Meng Zhang; Yuan Qin; Hengling Wei; Junji Su; Hantao Wang; Lijiao Gu; Nuohan Wang; Guoyuan Liu; Shuxun Yu. 2018. "Genome-wide identification and expression analyses of the pectate lyase (PEL) gene family in cotton (Gossypium hirsutum L.)." BMC Genomics 19, no. 1: 661.
WRKY transcription factors (TFs) participate in various physiological processes of plants. Although WRKY genes have been well studied in model plants, knowledge of the functional roles of these genes is still extremely limited in cotton. In this study, a group IId WRKY gene from cotton, GhWRKY42, was isolated and characterized. Our data showed that GhWRKY42 localized to the nucleus. A transactivation assay in yeast demonstrated that GhWRKY42 was not a transcriptional activator. A β-glucuronidase (GUS) activity assay revealed that the promoter of GhWRKY42 showed fragment deletion activity in Nicotiana tabacum and was mainly expressed in the roots, stems and leaves of ProGhWRKY42::GUS transgenic Arabidopsis plants. Quantitative real-time PCR (qRT-PCR) analysis indicated that GhWRKY42 was up-regulated during leaf senescence and was induced after exposure to abiotic stresses. Constitutive expression of GhWRKY42 in Arabidopsis led to a premature aging phenotype, which was correlated with an increased number of senescent leaves, reduced chlorophyll content and elevated expression of senescence-associated genes (SAGs). In addition, virus-induced gene silencing (VIGS) was used to silence the endogenous GhWRKY42 gene in cotton, and this silencing reduced plant height. Our findings indicate that GhWRKY42 is involved in abiotic stress responses, premature leaf senescence and stem development. This work establishes a solid foundation for further functional analysis of the GhWRKY42 gene in cotton.
Lijiao Gu; Hengling Wei; Hantao Wang; Junji Su; Shuxun Yu. Characterization and functional analysis of GhWRKY42, a group IId WRKY gene, in upland cotton (Gossypium hirsutum L.). BMC Genetics 2018, 19, 1 -14.
AMA StyleLijiao Gu, Hengling Wei, Hantao Wang, Junji Su, Shuxun Yu. Characterization and functional analysis of GhWRKY42, a group IId WRKY gene, in upland cotton (Gossypium hirsutum L.). BMC Genetics. 2018; 19 (1):1-14.
Chicago/Turabian StyleLijiao Gu; Hengling Wei; Hantao Wang; Junji Su; Shuxun Yu. 2018. "Characterization and functional analysis of GhWRKY42, a group IId WRKY gene, in upland cotton (Gossypium hirsutum L.)." BMC Genetics 19, no. 1: 1-14.