Dr. Ashutosh Sharma is a visiting research assistant professor at the Department of Chemistry, University of Illinois, Chicago. He completed his PhD studies in Chemistry from Central Drug Research Institute (CDRI) Luckow, India. One of his projects concerns the design and development of novel ratiometric fluorescent sensors and their application to quantitative imaging of diverse cellular signaling molecules. He describes an overview of the design principles for ratiometric fluorescent sensors and their application to simultaneous in situ quantitative imaging of two membrane lipids. He has published more than 15 papers in various journals and serves as an editorial member for Journal of Imaging, Journal of Chemistry, and ARKIVOC. His research interests inscribe the design and development of novel molecular fluorescent probes for imaging and analysis of cellular systems for targeting dysregulation in the signaling (downstream/upstream) pathways. Currently ongoing research work is based on the development of the novel drug candidate for the inhibition of membrane lipid-protein interaction. This inhibition of membrane lipid-protein leads to the development of novel drug candidate with exceptional efficacy with no off-target effect and no drug resistance.