Chiral N-heterocyclic molecules and in particular compounds with an amino functional group such as 3-aminopiperidine are valuable intermediates for the production of a large number of bioactive compounds with pharmacological properties. In this paper, the synthesis of both enantiomers of 3-amino-1-Boc-piperidine by amination of the prochiral precursor 1-Boc-3-piperidone using immobilized ω-transaminases (TAs-IMB), isopropylamine as amine donor and pyridoxal-5’-phosphate (PLP) as cofactor is described. Compared to other methods, the present approach affords the target compound in just one step with high yield and high enantiomeric excess starting from a commercial substrate. The reaction was carried out by using different commercially available immobilized enzymes, evaluating the catalytic activity and the enantioselectivity under different experimental conditions. Re-use of the most efficient enzyme was performed both in batch and in a semi-continuous system. The selected biocatalyst showed good stability under the reaction conditions providing consistent results in terms of conversion and enantiomeric excess after several cycles. The reported results may be of practical interest in view of the development of this sustainable approach to an industrial scale.

The hydroxylation of phenol with aqueous H₂O₂ to afford catechol and hydroquinone was studied in a biphasic reaction medium, as well as in pure water, in the presence of a bio-generated iron(III) catalyst. This catalyst was purified from a culture of Klebsiella oxytoca BAS-10 growing under anaerobic conditions, with Fe(III)-citrate as the energy and carbon source. The overproduction of an exopolymer (EPS) encrusted bacterial cells. The EPS, binding Fe³⁺ , (Fe-EPS), was extracted and studied before and after a reaction with phenol. Some of the reaction’s parameters, such as temperature, pH, and molar ratio between reagents and catalyst, were investigated to identify the best compromise between conversion and selectivity. The results could be useful either from a synthetic point of view or to support the biodegradation of aromatic substrates.