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Infrared thermography (IRT) is a non-ionizing, non-invasive technique that permits evaluating the comfort levels of animals, a topic of concern due to the growing interest in determining the state of health and welfare of production animals. The operating principle of IRT is detecting the heat irradiated in anatomical regions characterized by a high density of near-surface blood vessels that can regulate temperature gain or loss from/to the environment by modifying blood flow. This is essential for understanding the various vascular thermoregulation mechanisms of different species, such as rodents and ruminants’ tails. The usefulness of ocular, nasal, and vulvar thermal windows in the orbital (regio orbitalis), nasal (regio nasalis), and urogenital (regio urogenitalis) regions, respectively, has been demonstrated in cattle. However, recent evidence for the river buffalo has detected discrepancies in the data gathered from distinct thermal regions in these large ruminants, suggesting a limited sensitivity and specificity when used with this species due to various factors: the presence of hair, ambient temperature, and anatomical features, such as skin thickness and variations in blood supplies to different regions. In this review, a literature search was conducted in Scopus, Web of Science, ScienceDirect, and PubMed, using keyword combinations that included “infrared thermography”, “water buffalo”, “river buffalo” “thermoregulation”, “microvascular changes”, “lacrimal caruncle”, “udder”, “mastitis”, and “nostril”. We discuss recent findings on four thermal windows—the orbital and nasal regions, mammary gland in the udder region (regio uberis), and vulvar in the urogenital region (regio urogenitalis)—to elucidate the factors that modulate and intervene in validating thermal windows and interpreting the information they provide, as it relates to the clinical usefulness of IRT for cattle (Bos) and the river buffalo (Bubalus bubalis).
Daniel Mota-Rojas; Alfredo Pereira; Dehua Wang; Julio Martínez-Burnes; Marcelo Ghezzi; Ismael Hernández-Avalos; Pamela Lendez; Patricia Mora-Medina; Alejandro Casas; Adriana Olmos-Hernández; Adriana Domínguez; Aldo Bertoni; Ana Geraldo. Clinical Applications and Factors Involved in Validating Thermal Windows Used in Infrared Thermography in Cattle and River Buffalo to Assess Health and Productivity. Animals 2021, 11, 2247 .
AMA StyleDaniel Mota-Rojas, Alfredo Pereira, Dehua Wang, Julio Martínez-Burnes, Marcelo Ghezzi, Ismael Hernández-Avalos, Pamela Lendez, Patricia Mora-Medina, Alejandro Casas, Adriana Olmos-Hernández, Adriana Domínguez, Aldo Bertoni, Ana Geraldo. Clinical Applications and Factors Involved in Validating Thermal Windows Used in Infrared Thermography in Cattle and River Buffalo to Assess Health and Productivity. Animals. 2021; 11 (8):2247.
Chicago/Turabian StyleDaniel Mota-Rojas; Alfredo Pereira; Dehua Wang; Julio Martínez-Burnes; Marcelo Ghezzi; Ismael Hernández-Avalos; Pamela Lendez; Patricia Mora-Medina; Alejandro Casas; Adriana Olmos-Hernández; Adriana Domínguez; Aldo Bertoni; Ana Geraldo. 2021. "Clinical Applications and Factors Involved in Validating Thermal Windows Used in Infrared Thermography in Cattle and River Buffalo to Assess Health and Productivity." Animals 11, no. 8: 2247.
Extensive livestock production in Mediterranean climate conditions and acidic soils requires animal feed supplementation. This occurs during the summer and, frequently, also in the autumn and winter, depending on the prevailing rainfall patterns. The purpose of this study was to evaluate the effect of dolomitic limestone application and of tree canopy on availability, quality, and floristic composition of a permanent pasture, grazed by sheep. At the end of autumn, winter, and spring of 2018/2019 and 2019/2020 pasture green and dry matter production (GM and DM, respectively), crude protein (CP), and fiber (neutral detergent fiber) were monitored in 24 sampling points. Half of these points were located in areas amended with dolomitic limestone (COR) and half in unamended areas (UCOR). In each of these, half of the sampling points were located under tree canopy (UTC) and half outside tree canopy (OTC). Pasture floristic composition was monitored in spring 2020. The results show, in autumn, a positive and significant effect (i) of soil pH amendment on pasture DM and CP daily growth rate (kg·ha−1·day−1) (+28.8% and +42.6%, respectively), and (ii) of tree canopy on pasture CP daily growth rate (+26.4%). Both factors affect pasture floristic composition. Pasture species were identified as potential bio-indicators, characteristic of each field area. These results show the practical interest of the soil pH correction to reduce the animal supplementation needs in the critical autumn period in the Mediterranean montado ecosystem.
João Serrano; Shakib Shahidian; Francisco Costa; Emanuel Carreira; Alfredo Pereira; Mário Carvalho. Can Soil pH Correction Reduce the Animal Supplementation Needs in the Critical Autumn Period in Mediterranean Montado Ecosystem? Agronomy 2021, 11, 514 .
AMA StyleJoão Serrano, Shakib Shahidian, Francisco Costa, Emanuel Carreira, Alfredo Pereira, Mário Carvalho. Can Soil pH Correction Reduce the Animal Supplementation Needs in the Critical Autumn Period in Mediterranean Montado Ecosystem? Agronomy. 2021; 11 (3):514.
Chicago/Turabian StyleJoão Serrano; Shakib Shahidian; Francisco Costa; Emanuel Carreira; Alfredo Pereira; Mário Carvalho. 2021. "Can Soil pH Correction Reduce the Animal Supplementation Needs in the Critical Autumn Period in Mediterranean Montado Ecosystem?" Agronomy 11, no. 3: 514.
The Montado ecosystem, predominant in the Mediterranean region, consists of poor soils, a sparse cover of cork and holm with an understory of natural biodiverse pastures, grazed by animals in extensive regime. The recommended procedure for increasing productivity of these pastures is based on the application of phosphate fertilizer. One of the main productivity-limiting factors is, however, associated with soil acidity. The objective of this work was to evaluate the simultaneous effect of the holm oak canopy and the application of dolomitic lime on the productivity and quality of a permanent biodiverse pasture, grazed by sheep, in an acid soil (pH = 5.4 ± 0.3). Pasture was monitored at the end of autumn 2018 and winter and spring 2019. The results show that amendment of soil acidity is a slow and gradual process that improves soil Mg/Mn ratio and has a positive impact on pasture productivity and quality. Pasture crude protein availability (CP, kg·ha−1), which is based on both pasture dry matter yield (kg·ha−1) and quality (CP, %), proved to be a very practical indicator of the contributions of tree canopy and soil acidity correction to the holistic management of the Montado ecosystem.
João Serrano; Shakib Shahidian; José Marques Da Silva; Francisco Moral; Fernando Carvajal-Ramirez; Emanuel Carreira; Alfredo Pereira; Mário De Carvalho. Evaluation of the Effect of Dolomitic Lime Application on Pastures—Case Study in the Montado Mediterranean Ecosystem. Sustainability 2020, 12, 3758 .
AMA StyleJoão Serrano, Shakib Shahidian, José Marques Da Silva, Francisco Moral, Fernando Carvajal-Ramirez, Emanuel Carreira, Alfredo Pereira, Mário De Carvalho. Evaluation of the Effect of Dolomitic Lime Application on Pastures—Case Study in the Montado Mediterranean Ecosystem. Sustainability. 2020; 12 (9):3758.
Chicago/Turabian StyleJoão Serrano; Shakib Shahidian; José Marques Da Silva; Francisco Moral; Fernando Carvajal-Ramirez; Emanuel Carreira; Alfredo Pereira; Mário De Carvalho. 2020. "Evaluation of the Effect of Dolomitic Lime Application on Pastures—Case Study in the Montado Mediterranean Ecosystem." Sustainability 12, no. 9: 3758.
Climate change is seen as a significant threat to the sustainability of livestock production systems in many parts of the world, particularly in tropical regions. Extreme meteorological events can result in catastrophic production and death of livestock. Heat waves in particular can push vulnerable animals beyond their survival threshold limits. However, there is little information about buffalo responses to sudden changes in the thermal environment, specifically the heat waves. This study aimed to quantify the thermoregulatory and blood biochemical responses of heat-acclimatized buffaloes to a simulated heat wave. The experiment was designed in a climatic chamber with two periods of 4 days each. Twelve heat acclimated buffalo heifers aged 18 months were used. The climatic chamber environment was set as follows: 4-day period (P1) simulating the same weather conditions of a summer in humid tropical climate used as a baseline, with daily cycle with Ta and RH at 27 ± 1 °C and 76% from 0600 h to 1900 h and 24 ± 1 °C and 80% from 1900 h to 0600 h, and 4-day period (P2), simulating a daily heat wave cycle, from 0600 h to 1900 h with Ta and RH kept at 36 °C and 78% and from 1900 h to 0600 h, 27 °C and 74%. All animals were subject to both treatments and data were analyzed by a repeated measure analysis of variance, with post-hoc pooling comparison performed by Tukey’s test. In P2, there was observed a significant increase in respiratory frequency (p < 0.01), found four times in P1. The sweating rates were quite high in both periods; still, there were significant increases in P2 compared to P1 (p < 0.01) (4931 and 3201 g/m2/h, respectively). A slight but significant increase in rectal temperature was observed during the day (p < 0.01), with a rising until 1900 h. The simulated heat wave in P2 did not affect the values of the erythrogram or leukogram, excluding the significant reduction in K+ (p < 0.05). The low heat storage and the subsequent fast and full recovery of the thermal balance late afternoon appear to be related to the high sweating rate values. The massive sweating rate emphasizes its relevance in the maintenance of buffalo homeothermy. The absence of changes in hematological parameters has revealed the considerable physiological resilience of buffaloes toward simulated heat waves.
Alfredo M. F. Pereira; Reíssa A. Vilela; Cristiane G. Titto; Thays M. C. Leme-Dos-Santos; Ana C. M. Geraldo; Júlio C. C. Balieiro; Raquel F. Calviello; Eduardo H. Birgel Junior; Evaldo Titto. Thermoregulatory Responses of Heat Acclimatized Buffaloes to Simulated Heat Waves. Animals 2020, 10, 756 .
AMA StyleAlfredo M. F. Pereira, Reíssa A. Vilela, Cristiane G. Titto, Thays M. C. Leme-Dos-Santos, Ana C. M. Geraldo, Júlio C. C. Balieiro, Raquel F. Calviello, Eduardo H. Birgel Junior, Evaldo Titto. Thermoregulatory Responses of Heat Acclimatized Buffaloes to Simulated Heat Waves. Animals. 2020; 10 (5):756.
Chicago/Turabian StyleAlfredo M. F. Pereira; Reíssa A. Vilela; Cristiane G. Titto; Thays M. C. Leme-Dos-Santos; Ana C. M. Geraldo; Júlio C. C. Balieiro; Raquel F. Calviello; Eduardo H. Birgel Junior; Evaldo Titto. 2020. "Thermoregulatory Responses of Heat Acclimatized Buffaloes to Simulated Heat Waves." Animals 10, no. 5: 756.
Montado is an agro-silvo-pastoral system characterized by a high complexity as a result of the interactions between climate, soil, pasture, trees, and animals. It is in this context that management decisions must be made, for example with respect to soil fertilization, grazing, or animal supplementation. In this work, the effect of the tree canopy on the spatial and temporal variability of the soil and productivity, quality, and floristic composition of the pasture was evaluated. Precision agriculture (PA) technologies for monitoring soil and pasture were also evaluated. The study was carried out between October 2015 and June 2018 in an experimental field of 2.3 ha. The results showed: (i) The positive impact of trees and animal grazing on soil fertility; (ii) the influence of inter-annual variability of precipitation on the pattern of pasture vegetative cycle; (iii) the positive effect of trees in pasture quality; (iv) the negative effect of trees in pasture productivity; (v) the role of pasture floristic composition as an indicator of soil limitations or climatic changes; (vi) the potential of technologies associated with the concept of PA as express tools to decision making support and for the optimization of the herbaceous stratum and the dynamic management of grazing in this ecosystem in a holistic and sustainable form.
João Serrano; Shakib Shahidian; José Marques Da Silva; Luís Paixão; Emanuel Carreira; Alfredo Pereira; Mário Carvalho. Climate Changes Challenges to the Management of Mediterranean Montado Ecosystem: Perspectives for Use of Precision Agriculture Technologies. Agronomy 2020, 10, 218 .
AMA StyleJoão Serrano, Shakib Shahidian, José Marques Da Silva, Luís Paixão, Emanuel Carreira, Alfredo Pereira, Mário Carvalho. Climate Changes Challenges to the Management of Mediterranean Montado Ecosystem: Perspectives for Use of Precision Agriculture Technologies. Agronomy. 2020; 10 (2):218.
Chicago/Turabian StyleJoão Serrano; Shakib Shahidian; José Marques Da Silva; Luís Paixão; Emanuel Carreira; Alfredo Pereira; Mário Carvalho. 2020. "Climate Changes Challenges to the Management of Mediterranean Montado Ecosystem: Perspectives for Use of Precision Agriculture Technologies." Agronomy 10, no. 2: 218.
The present work describes the microtomographic characterization of macro and microcalcifications present in excised canine mammary glands. In human breast cancer, microcalcifications are highly relevant for diagnosis and prognosis, often being the sole element determining biopsy. Canine mammary tumours are considered a model for human breast cancer, but the morphological features of calcifications had still to be studied in this species. The objective of this research is to contribute to the characterization of the mineralization features of the canine mammary gland. In the present study, the excised mammary glands of 33 bitches underwent fluoroscopic examination. In 30 of the samples, the presence of calcification was suspected, and multiple biopsies were taken of these areas. Biopsy fragments underwent microtomographic scanning. Microcalcifications were found in non‐neoplastic glandular tissue, benign and malign lesions, as it is known to happen in humans. Qualitative evaluation regarding morphology of the imaged calcifications showed similarities to breast cancer findings, based on the BI‐RADS 2013 classification, such as pleomorphism and shape. No differences in the quantitative morphological parameters of volume, surface, surface/volume, SMI and structure thickness were found when macrocalcifications were considered. However, although significant differences existed in these parameters between microcalcifications from malignant canine mammary tumours and the two other groups, none were found between non‐neoplastic and benign tumours. Findings further support the use of this spontaneous animal model for the study of human breast cancer, considering how clinically relevant microcalcifications are in humans. This article is protected by copyright. All rights reserved.
Joana Reis; Teresa Oliveira; Alfredo Pereira; Paulo Infante; Nuno Leal; Pedro Faísca. Microtomographic characterization of calcifications in canine mammary tumours. Veterinary and Comparative Oncology 2019, 18, 281 -291.
AMA StyleJoana Reis, Teresa Oliveira, Alfredo Pereira, Paulo Infante, Nuno Leal, Pedro Faísca. Microtomographic characterization of calcifications in canine mammary tumours. Veterinary and Comparative Oncology. 2019; 18 (3):281-291.
Chicago/Turabian StyleJoana Reis; Teresa Oliveira; Alfredo Pereira; Paulo Infante; Nuno Leal; Pedro Faísca. 2019. "Microtomographic characterization of calcifications in canine mammary tumours." Veterinary and Comparative Oncology 18, no. 3: 281-291.
Adaptation is a relevant characteristic to be understood in livestock animals in order to maintain and raise productivity. In Brazil, the Nellore beef cattle are widely disseminated and well-adapted breed that present good thermoregulatory characteristics for tropical environment conditions. Conversely, the physiological and cellular mechanisms required for thermoregulation and thermotolerance in this breed are still limited. The aim of this study was to comprehend the heat loss efficiency at the whole animal level and heat shock response at the cellular level of Nellore cows in tropical climate conditions. Healthy purebred Nellore cows were classified according to their capacity to lose body heat as Efficient or Inefficient based on vaginal temperature which was continuously monitored by data-loggers. Rectal, tail, and ocular temperatures, sweating rate, and respiratory frequency were collected to assess other thermoregulatory responses. Peripheral mononuclear cells were used for gene expression of heat shock proteins 60, 70, and 90 induced by in vitro heat treatments at 38, 40, and 42 °C. In our findings, the Efficient cows presented higher sweating rates compared to Inefficient cows that presented higher rectal temperature with greater amplitude of vaginal temperature profile. Transcription of the HSP genes was stable at 38 and 40 °C and decreased for all HSP genes at 42 °C. In conclusion, the Nellore efficiency to lose heat was mainly associated with their sweating capacity and cellular thermotolerance confirmed by the maintenance of heat shock proteins transcripts under heat stress. Taken together, this knowledge contributes as a future key for genetic selection of adapted animals.
Henrique Barbosa Hooper; Cristiane Gonçalves Titto; Angela Maria Gonella-Diaza; Fábio Luís Henrique; Lina Fernanda Pulido-Rodríguez; Ana Luisa Silva Longo; Thays Mayra Da Cunha Leme-Dos-Santos; Ana Carina Alves Pereira De Mira Geraldo; Alfredo Manuel Franco Pereira; Mario Binelli; Júlio Cesar De Carvalho Balieiro; Evaldo Antonio Lencioni Titto. Heat loss efficiency and HSPs gene expression of Nellore cows in tropical climate conditions. International Journal of Biometeorology 2018, 63, 1475 -1486.
AMA StyleHenrique Barbosa Hooper, Cristiane Gonçalves Titto, Angela Maria Gonella-Diaza, Fábio Luís Henrique, Lina Fernanda Pulido-Rodríguez, Ana Luisa Silva Longo, Thays Mayra Da Cunha Leme-Dos-Santos, Ana Carina Alves Pereira De Mira Geraldo, Alfredo Manuel Franco Pereira, Mario Binelli, Júlio Cesar De Carvalho Balieiro, Evaldo Antonio Lencioni Titto. Heat loss efficiency and HSPs gene expression of Nellore cows in tropical climate conditions. International Journal of Biometeorology. 2018; 63 (11):1475-1486.
Chicago/Turabian StyleHenrique Barbosa Hooper; Cristiane Gonçalves Titto; Angela Maria Gonella-Diaza; Fábio Luís Henrique; Lina Fernanda Pulido-Rodríguez; Ana Luisa Silva Longo; Thays Mayra Da Cunha Leme-Dos-Santos; Ana Carina Alves Pereira De Mira Geraldo; Alfredo Manuel Franco Pereira; Mario Binelli; Júlio Cesar De Carvalho Balieiro; Evaldo Antonio Lencioni Titto. 2018. "Heat loss efficiency and HSPs gene expression of Nellore cows in tropical climate conditions." International Journal of Biometeorology 63, no. 11: 1475-1486.
Montado is a silvo-pastoral ecosystem of the Mediterranean region, a mixed system of trees and grass, where livestock graze. The information about the spatial and temporal variability of pastures constitutes the basis to estimate available feed, a fundamental decision support tool for the farm manager to define the animal stocking or the rotation of the grazed paddocks. In this study, the intrinsic features of high spatial–temporal variability of Mediterranean grazed pastures were assessed with the objective of evaluating the suitability of two proximal sensing techniques (an active optical sensor, AOS and a capacitance probe) for easily monitoring seasonal variability of pasture productivity and quality linked to animal grazing patterns. The correlation between pasture and sensor parameters was consistent between capacitance and pasture productivity (r2 = 0.68, P < 0.01; and r2 = 0.87, P < 0.01, respectively for green pasture biomass, PB and pasture moisture content, PMC), between NDVI and pasture productivity (r2 = 0.73, P < 0.01; and r2 = 0.96, P < 0.01, respectively for PB and PMC) and between NDVI and pasture quality (r2 = 0.44, P < 0.05; r2 = 0.69, P < 0.01; and r2 = 0.78, P < 0.01, respectively for ash, crude protein, CP and neutral detergent fibre, NDF). The approach is a promising methodology for assessing seasonal changes in pasture that have values of biomass that range between 2000 and 85,000 kg ha−1 and vegetative sates from de green and leafy to dry. These results can be an important starting point for studies of evaluation and calibration of the optical sensor specifically for pasture quality assessment in different types of biodiverse pastures. This is a key factor for the management of animal grazing intensity and calculation of feed supplementation needs.
João Serrano; Elvira Sales-Baptista; Shakib Shahidian; J. Marques da Silva; I. Ferraz de Oliveira; J. Lopes de Castro; Alfredo Pereira; M. Cancela D’Abreu; Mário de Carvalho. Proximal sensors for monitoring seasonal changes of feeding sites selected by grazing ewes. Agroforestry Systems 2018, 95, 55 -69.
AMA StyleJoão Serrano, Elvira Sales-Baptista, Shakib Shahidian, J. Marques da Silva, I. Ferraz de Oliveira, J. Lopes de Castro, Alfredo Pereira, M. Cancela D’Abreu, Mário de Carvalho. Proximal sensors for monitoring seasonal changes of feeding sites selected by grazing ewes. Agroforestry Systems. 2018; 95 (1):55-69.
Chicago/Turabian StyleJoão Serrano; Elvira Sales-Baptista; Shakib Shahidian; J. Marques da Silva; I. Ferraz de Oliveira; J. Lopes de Castro; Alfredo Pereira; M. Cancela D’Abreu; Mário de Carvalho. 2018. "Proximal sensors for monitoring seasonal changes of feeding sites selected by grazing ewes." Agroforestry Systems 95, no. 1: 55-69.
Fábio Luís Henrique; Cristiane Gonçalves Titto; Evaldo Antonio Lencioni Titto; Henrique Barbosa Hooper; Lina Fernanda Pulido-Rodríguez; Ana Luísa Silva Longo; Thays Mayra Da Cunha Leme-Dos-Santos; Augusto Hauber Gameiro; Alfredo Pereira; Adroaldo José Zanella. Abrupt weaning type combined to stress during late pregnancy in sheep present economic losses on carcass and low testicular development in lambs. Journal of Animal Behaviour and Biometeorology 2018, 6, 1 .
AMA StyleFábio Luís Henrique, Cristiane Gonçalves Titto, Evaldo Antonio Lencioni Titto, Henrique Barbosa Hooper, Lina Fernanda Pulido-Rodríguez, Ana Luísa Silva Longo, Thays Mayra Da Cunha Leme-Dos-Santos, Augusto Hauber Gameiro, Alfredo Pereira, Adroaldo José Zanella. Abrupt weaning type combined to stress during late pregnancy in sheep present economic losses on carcass and low testicular development in lambs. Journal of Animal Behaviour and Biometeorology. 2018; 6 (2):1.
Chicago/Turabian StyleFábio Luís Henrique; Cristiane Gonçalves Titto; Evaldo Antonio Lencioni Titto; Henrique Barbosa Hooper; Lina Fernanda Pulido-Rodríguez; Ana Luísa Silva Longo; Thays Mayra Da Cunha Leme-Dos-Santos; Augusto Hauber Gameiro; Alfredo Pereira; Adroaldo José Zanella. 2018. "Abrupt weaning type combined to stress during late pregnancy in sheep present economic losses on carcass and low testicular development in lambs." Journal of Animal Behaviour and Biometeorology 6, no. 2: 1.
Montado is a silvo-pastoral ecosystem of the Mediterranean region, a mixed system of trees and pasture, subject to animal grazing. Farmers need information on pasture production and quality in order to assess the direct effect of tree presence on the productivity of their pastoral system, and to devise management that balances farm production and profitability with sustainable soil management. The main objectives of this work were (1) to evaluate tree influence on soil and pasture parameters and (2) to evaluate the use of proximal sensing techniques that have potential for monitoring aspects related to spatial and temporal variability of pasture productivity and quality in montado ecosystems. Both objectives can support the decision-making process of the farmer. The study field is located in Mitra farm, in Southern Portugal. During October 2015, 24 geo-referenced composite soil samples (12 under tree canopy and 12 outside tree canopy) were collected from the 0.0–0.3 m soil layer. The soil samples were analysed for texture (sand, silt, and clay content), moisture content, pH, organic matter, total nitrogen (N), phosphorus (P), potassium (K), magnesium (Mg), and manganese (Mn). The evolution of the pasture was recorded in the 24 sampling points at five monitoring dates: at the end of autumn (December 2015), at the end of winter (March 2016), and then monthly during spring 2016 (April, May, and June). The following pasture parameters were measured: normalized difference vegetation index (NDVI), capacitance, temperature, green and dry matter, ash, crude protein (CP), and neutral detergent fibre. Soil under tree canopy had significantly higher levels of organic matter, N, P, K, and Mg, and better pasture quality while the pasture productivity was higher outside tree canopy. The correlation between pasture direct measurements and sensor parameters was more consistent between capacitance and pasture productivity and between NDVI and CP. The use of fast and efficient tools associated with geo-referenced systems can greatly simplify the pasture monitoring process, which is the basis for estimating feed availability in the field. The knowledge of biomass quality and quantity is fundamental to support decision-making regarding animal stocking rates and rotation among grazing parcels.
João Serrano; Shakib Shahidian; J. Marques Da Silva; E. Sales-Baptista; I. Ferraz De Oliveira; J. Lopes De Castro; Alfredo Pereira; M. Cancela De Abreu; Eliana Machado; Mário de Carvalho. Tree influence on soil and pasture: contribution of proximal sensing to pasture productivity and quality estimation in montado ecosystems. International Journal of Remote Sensing 2017, 39, 4801 -4829.
AMA StyleJoão Serrano, Shakib Shahidian, J. Marques Da Silva, E. Sales-Baptista, I. Ferraz De Oliveira, J. Lopes De Castro, Alfredo Pereira, M. Cancela De Abreu, Eliana Machado, Mário de Carvalho. Tree influence on soil and pasture: contribution of proximal sensing to pasture productivity and quality estimation in montado ecosystems. International Journal of Remote Sensing. 2017; 39 (14):4801-4829.
Chicago/Turabian StyleJoão Serrano; Shakib Shahidian; J. Marques Da Silva; E. Sales-Baptista; I. Ferraz De Oliveira; J. Lopes De Castro; Alfredo Pereira; M. Cancela De Abreu; Eliana Machado; Mário de Carvalho. 2017. "Tree influence on soil and pasture: contribution of proximal sensing to pasture productivity and quality estimation in montado ecosystems." International Journal of Remote Sensing 39, no. 14: 4801-4829.
Fábio Luís Henrique; Evaldo Antonio Lencioni Titto; Adoraldo José Zanella; Henrique Barbosa Hooper; Lina Fernanda Pulido-Rodriguez; Ana Luísa Silva Longo; Thays Mayra Da Cunha Leme-Dos-Santos; Alfredo Manoel Franco Pereira; Cristiane Gonçalves Titto. Simulated disease process during late pregnancy compromises developmental outcomes of lambs independently of the weaning method applied. Small Ruminant Research 2017, 155, 39 -44.
AMA StyleFábio Luís Henrique, Evaldo Antonio Lencioni Titto, Adoraldo José Zanella, Henrique Barbosa Hooper, Lina Fernanda Pulido-Rodriguez, Ana Luísa Silva Longo, Thays Mayra Da Cunha Leme-Dos-Santos, Alfredo Manoel Franco Pereira, Cristiane Gonçalves Titto. Simulated disease process during late pregnancy compromises developmental outcomes of lambs independently of the weaning method applied. Small Ruminant Research. 2017; 155 ():39-44.
Chicago/Turabian StyleFábio Luís Henrique; Evaldo Antonio Lencioni Titto; Adoraldo José Zanella; Henrique Barbosa Hooper; Lina Fernanda Pulido-Rodriguez; Ana Luísa Silva Longo; Thays Mayra Da Cunha Leme-Dos-Santos; Alfredo Manoel Franco Pereira; Cristiane Gonçalves Titto. 2017. "Simulated disease process during late pregnancy compromises developmental outcomes of lambs independently of the weaning method applied." Small Ruminant Research 155, no. : 39-44.
This Research Communication describes, for the first time, the detection of HSP70 in saliva of dairy cows. Thermal stress is a major environmental stress that limits animal growth, metabolism, and productivity. The cellular response to heat stress involves the synthesis of heat shock proteins (HSPs), presumably to protect the functional stability of cells at increasing temperatures. HSP70 has been found to be present in cattle blood serum and may also be present in other secretory fluids, such as saliva, as already observed in humans. The aim of this study was to detect heat shock protein HSP70 in bovine saliva. Saliva samples were taken from higher- (n = 5) and lower milk producing (n = 5) Holstein-Friesian cows in summer and in winter for the detection of HSP70. HSP70 concentrations were assayed using the ELISA technique. Salivary HSP70 concentrations ranged from 0·524 to 12·174 ng/ml in cows. Higher salivary HSP70 concentrations were significantly associated with higher milk production and higher environmental temperature, but not with rectal temperature.
Elsa Lamy; Viktor Jurkovich; Lénia Rodrigues; Ana Geraldo; Liliana Cachucho; Flávio Daniel Gomes da Silva; Catarina Matos; Fernando Capela e Silva; Cristina Pinheiro; László Könyves; Mikolt Bakony; Alfredo Pereira. Detection of 70 kDa heat shock protein in the saliva of dairy cows. Journal of Dairy Research 2017, 84, 280 -282.
AMA StyleElsa Lamy, Viktor Jurkovich, Lénia Rodrigues, Ana Geraldo, Liliana Cachucho, Flávio Daniel Gomes da Silva, Catarina Matos, Fernando Capela e Silva, Cristina Pinheiro, László Könyves, Mikolt Bakony, Alfredo Pereira. Detection of 70 kDa heat shock protein in the saliva of dairy cows. Journal of Dairy Research. 2017; 84 (3):280-282.
Chicago/Turabian StyleElsa Lamy; Viktor Jurkovich; Lénia Rodrigues; Ana Geraldo; Liliana Cachucho; Flávio Daniel Gomes da Silva; Catarina Matos; Fernando Capela e Silva; Cristina Pinheiro; László Könyves; Mikolt Bakony; Alfredo Pereira. 2017. "Detection of 70 kDa heat shock protein in the saliva of dairy cows." Journal of Dairy Research 84, no. 3: 280-282.
RESUMO: Desmama é uma fase crítica na vida do suíno devido a separação materna e a introdução de uma dieta seca. A termografia infravermelha medida na região ocular se mostra como um indicador confiável para a condição de estresse pontual de suínos. O objetivo deste estudo foi determinar a relação entre medidas de termografia infravermelha da superfície ocular e de cortisol em situações de estresse em leitões na pós desmama. Foram avaliados 66 leitões, uma vez por semana, durante sete semanas, em dois períodos do dia (7h e 15h) com medidas de temperatura superficial ocular, temperatura superficial do dorso e amostras de saliva para determinação de cortisol salivar. A análise estatística contemplou os efeitos fixos de semana e período do dia e sua interação e correlações de Pearson para relação entre termografia ocular, temperatura superficial e cortisol a 5% de significância. Cortisol salivar não diferiu entre os períodos, mas foi superior nas três primeiras semanas após o desmame (P<0,05). Nas duas primeiras semanas após a desmama o cortisol apresentou correlação alta e positiva (P<0,05) com a temperatura ocular máxima (0,89) e a temperatura superficial do dorso (0,80). As duas temperaturas superficiais apresentaram uma associação moderada positiva (r=0,41; P<0,0001) durante todo o período experimental. Este estudo destaca que a temperatura de superfície ocular obtida por meio da termografia infravermelha pode ser um indicador de temperatura de superfície corporal e estado de bem-estar de leitões em fase de creche, além de ser um método não invasivo e de rápida mensuração. Entretanto, mais pesquisas são necessárias para aprofundar a relação entre temperatura ocular e cortisol durante estresse crônico. TERMOS DE INDEXAÇÃO: Termografia infravermelha; superfície ocular; indicador de estresse; suínos na fase de creche; bem-estar animal; cortisol salivar; desmama; imagem infravermelha; temperatura de superfície corporal.
Lina Pulido Rodriguez; Evaldo Titto; Fábio Luís Henrique; Ana Luisa Silva Longo; Henrique Barbosa Hooper; Thuanny Lúcia Pereira. Termografia infravermelha da superfície ocular como indicador de estresse em suínos na fase de creche. Pesquisa Veterinária Brasileira 2017, 37, 453 -458.
AMA StyleLina Pulido Rodriguez, Evaldo Titto, Fábio Luís Henrique, Ana Luisa Silva Longo, Henrique Barbosa Hooper, Thuanny Lúcia Pereira. Termografia infravermelha da superfície ocular como indicador de estresse em suínos na fase de creche. Pesquisa Veterinária Brasileira. 2017; 37 (5):453-458.
Chicago/Turabian StyleLina Pulido Rodriguez; Evaldo Titto; Fábio Luís Henrique; Ana Luisa Silva Longo; Henrique Barbosa Hooper; Thuanny Lúcia Pereira. 2017. "Termografia infravermelha da superfície ocular como indicador de estresse em suínos na fase de creche." Pesquisa Veterinária Brasileira 37, no. 5: 453-458.
This study aimed to evaluate the effect of diets containing increasing levels of citrus pulp on the physicochemical and microbiological characteristics of horses feces. Five mares, at an average age of 3.5 years old and body weight of 492 ± 44.5 kg were arranged in a 5 x 5 Latin Square. The experimental diet consisted of 60% coast-cross hay and 40 % of concentrate with increasing levels of citrus pulp (0, 7, 14, 21, and 28 %). To determine the fecal pH, samples were collected directly from the floor, immediately after defecation, in the first feces of the day at 07:00 a.m., and color and fecal consistency were evaluated. For microbiological analysis, an aliquot was reserved in plastic bags, frozen, and sent to the microbiological laboratory for further analysis. Lactic acid bacteria were counted for Lactobacillus spp. and Streptococcus spp. from fecal samples under anaerobic conditions. The diet produced differences (P0.05) on pH and on the number of Lactobacillus spp. and Streptococcus spp. The inclusion of up to 28% citrus pulp concentrates for horses did not promote change in the physicochemical characteristics and on the population of lactic acid-producing bacteria in feces.
Roberta Ariboni Brandi; Adriana Moraes De Oliveira Tribucci; Júlio César De Carvalho Balieiro; Ricardo Luiz Moro De Souza; Alfredo Manuel Franco Pereira; Rhonda Hoffman. PHYSICOCHEMICAL AND MICROBIAL ANALYSIS OF FECES FROM HORSES FED DIETS CONTAINING CITRUS PULP. Ciência Animal Brasileira 2016, 17, 527 -533.
AMA StyleRoberta Ariboni Brandi, Adriana Moraes De Oliveira Tribucci, Júlio César De Carvalho Balieiro, Ricardo Luiz Moro De Souza, Alfredo Manuel Franco Pereira, Rhonda Hoffman. PHYSICOCHEMICAL AND MICROBIAL ANALYSIS OF FECES FROM HORSES FED DIETS CONTAINING CITRUS PULP. Ciência Animal Brasileira. 2016; 17 (4):527-533.
Chicago/Turabian StyleRoberta Ariboni Brandi; Adriana Moraes De Oliveira Tribucci; Júlio César De Carvalho Balieiro; Ricardo Luiz Moro De Souza; Alfredo Manuel Franco Pereira; Rhonda Hoffman. 2016. "PHYSICOCHEMICAL AND MICROBIAL ANALYSIS OF FECES FROM HORSES FED DIETS CONTAINING CITRUS PULP." Ciência Animal Brasileira 17, no. 4: 527-533.
This study aimed to identify the parameters related to the expression of the reactivity in horses during handling and based on that proposed and validated a scale of composite measure reactivity score to characterize horse's reactivity. To this end, the first stage (S1) proposed the scale and the second (S2) validated it. In S1, 364 Lusitano horses were evaluated, 188 were adult breeding mares (4–12 years old), and 176 were foals (males/females, aged from 2 months to 2 years). During hooves trimming, vermifuge application, palpation scores were assigned to behaviors of movement, ears and eyes position, breathing, vocalization, and urination. A response parameter called reactivity was attributed to each animal, ranging from score 1 (nonreactive/calm) to score 4 (very reactive/aggressive). The verification of the possible parameters (age, behavior), which explains the response parameter (reactivity), was taken using ordinal proportional odds model. Movement, breathing, ears and eyes position, vocalization, and age appear to explain the reactivity of horses during handling (P < .01). Therefore, based on these parameters, it was possible to propose two scales of composite measure reactivity score: one to characterize the mares and another the foals. On S2, the proposed scale was validated by the simultaneous application of Forced Human Approach Test, another commonly used test to evaluate the reactivity in horses, with a correlation of 0.97 (P < .05). The assessment of the reactivity of horses during handling by a composite measure reactivity score scale is valid, and easy to apply, without disrupting daily routine and override the impact of individual differences
Raquel Ferrari Calviello; Evaldo Titto; Paulo Infante; Thays Mayra Da Cunha Leme-Dos-Santos; Marcos Chiquitelli Neto; Alfredo Manuel Franco Pereira; Cristiane Titto. Proposal and Validation of a Scale of Composite Measure Reactivity Score to Characterize the Reactivity in Horses During Handling. Journal of Equine Veterinary Science 2016, 47, 62 -70.
AMA StyleRaquel Ferrari Calviello, Evaldo Titto, Paulo Infante, Thays Mayra Da Cunha Leme-Dos-Santos, Marcos Chiquitelli Neto, Alfredo Manuel Franco Pereira, Cristiane Titto. Proposal and Validation of a Scale of Composite Measure Reactivity Score to Characterize the Reactivity in Horses During Handling. Journal of Equine Veterinary Science. 2016; 47 ():62-70.
Chicago/Turabian StyleRaquel Ferrari Calviello; Evaldo Titto; Paulo Infante; Thays Mayra Da Cunha Leme-Dos-Santos; Marcos Chiquitelli Neto; Alfredo Manuel Franco Pereira; Cristiane Titto. 2016. "Proposal and Validation of a Scale of Composite Measure Reactivity Score to Characterize the Reactivity in Horses During Handling." Journal of Equine Veterinary Science 47, no. : 62-70.
This study’s main goal was to evaluate the thermoregulatory responses velocity through the variation of rectal temperature (RT), related to the thermolytic pathways, respiratory rate (RR) and sweating rate (SR) among different sheep breeds. Ninety female sheep, eighteen of each breed: Santa Ines and Morada Nova (Brazilian hair breeds), Texel, Suffolk and Ile de France (wool breeds) were challenged during three non-consecutive summer days (22◦42′S, 47◦18′W, and 570m of altitude, maximum air temperature of 33.5◦C, average relative humidity of 52±6.9%). The physiological variables were registered at 0800h (T1), 1300 h (T2: after 2 h of shade rest), 1400 h (T3) (after one hour of sun exposure) and in the shade at 1415 h (T4), 1430 h (T5), 1445 h (T6) and 1500 h (T7) and a thermotolerance index (TCI) was calculated as (10-(T7 to T4)-T1). The statistical analysis was performed by a mathematical model including the fixed effects of breeds and time frames, and the interaction between these effects, besides random effects such as animal and day. The Santa Ines breed presented the lowest RT after sun exposure (39.3 ± 0.12 ◦ C; P < 0.05) and it was the only one to recover morning RT 60 min after heat stress (38.7 and 38.9 for 1300 h and 1500 h; P > 0.05). Hair breeds presented RR lower (P < 0.05) than wool breeds. Although thick wool or hair thickness differs among and within hair and wool breeds (P < 0.05), SR did not differ among breeds and time (227.7 ± 16.44 g m−2 h−1 ; P > 0.05). The thermotolerance index did not differ among breeds, but it showed similar response (P > 0.05) 45 min or 1 h of shade after sun exposure. One week post shearing is not enough to wool breeds present to show thermotolerance similar to hair breeds
Cristiane Gonçalves Titto; Cecília José Veríssimo; Alfredo Manuel Franco Pereira; Ana De Mira Geraldo; Luciana Morita Katiki; Evaldo Antonio Lencioni Titto. Thermoregulatory response in hair sheep and shorn wool sheep. Small Ruminant Research 2016, 144, 341 -345.
AMA StyleCristiane Gonçalves Titto, Cecília José Veríssimo, Alfredo Manuel Franco Pereira, Ana De Mira Geraldo, Luciana Morita Katiki, Evaldo Antonio Lencioni Titto. Thermoregulatory response in hair sheep and shorn wool sheep. Small Ruminant Research. 2016; 144 ():341-345.
Chicago/Turabian StyleCristiane Gonçalves Titto; Cecília José Veríssimo; Alfredo Manuel Franco Pereira; Ana De Mira Geraldo; Luciana Morita Katiki; Evaldo Antonio Lencioni Titto. 2016. "Thermoregulatory response in hair sheep and shorn wool sheep." Small Ruminant Research 144, no. : 341-345.
Percutaneous vertebroplasty (PVP) is a minimally invasive surgical procedure and is frequently performed in humans who need surgical treatment of vertebral fractures. PVP involves cement injection into the vertebral body, thereby providing rapid and significant pain relief.
Maria Teresa Oliveira; José Potes; Maria Cristina Queiroga; José L. Castro; Alfredo F. Pereira; Sarrawat Rehman; Kenneth Dalgarno; António Ramos; Chiara Vitale-Brovarone; Joana C. Reis. Percutaneous vertebroplasty: a new animal model. The Spine Journal 2016, 16, 1253 -1262.
AMA StyleMaria Teresa Oliveira, José Potes, Maria Cristina Queiroga, José L. Castro, Alfredo F. Pereira, Sarrawat Rehman, Kenneth Dalgarno, António Ramos, Chiara Vitale-Brovarone, Joana C. Reis. Percutaneous vertebroplasty: a new animal model. The Spine Journal. 2016; 16 (10):1253-1262.
Chicago/Turabian StyleMaria Teresa Oliveira; José Potes; Maria Cristina Queiroga; José L. Castro; Alfredo F. Pereira; Sarrawat Rehman; Kenneth Dalgarno; António Ramos; Chiara Vitale-Brovarone; Joana C. Reis. 2016. "Percutaneous vertebroplasty: a new animal model." The Spine Journal 16, no. 10: 1253-1262.
Elvira Sales-Baptista; Maria Isabel Ferraz-De-Oliveira; Margarida Brandão Dos Santos; José António Lopes De Castro; Alfredo Pereira; José R. Marques Da Silva; João Serrano. Tecnologia GNSS de baixo custo na monitorização de ovinos em pastoreio. Revista de Ciências Agrárias 2016, 39, 251 -260.
AMA StyleElvira Sales-Baptista, Maria Isabel Ferraz-De-Oliveira, Margarida Brandão Dos Santos, José António Lopes De Castro, Alfredo Pereira, José R. Marques Da Silva, João Serrano. Tecnologia GNSS de baixo custo na monitorização de ovinos em pastoreio. Revista de Ciências Agrárias. 2016; 39 (2):251-260.
Chicago/Turabian StyleElvira Sales-Baptista; Maria Isabel Ferraz-De-Oliveira; Margarida Brandão Dos Santos; José António Lopes De Castro; Alfredo Pereira; José R. Marques Da Silva; João Serrano. 2016. "Tecnologia GNSS de baixo custo na monitorização de ovinos em pastoreio." Revista de Ciências Agrárias 39, no. 2: 251-260.
Catarina Lavrador; Ramiro Mascarenhas; Paulo Coelho; Cláudia Brites; Alfredo Pereira; Sylwester Gogolewski. Elastomeric enriched biodegradable polyurethane sponges for critical bone defects: a successful case study reducing donor site morbidity. Journal of Materials Science: Materials in Electronics 2016, 27, 1 -12.
AMA StyleCatarina Lavrador, Ramiro Mascarenhas, Paulo Coelho, Cláudia Brites, Alfredo Pereira, Sylwester Gogolewski. Elastomeric enriched biodegradable polyurethane sponges for critical bone defects: a successful case study reducing donor site morbidity. Journal of Materials Science: Materials in Electronics. 2016; 27 (3):1-12.
Chicago/Turabian StyleCatarina Lavrador; Ramiro Mascarenhas; Paulo Coelho; Cláudia Brites; Alfredo Pereira; Sylwester Gogolewski. 2016. "Elastomeric enriched biodegradable polyurethane sponges for critical bone defects: a successful case study reducing donor site morbidity." Journal of Materials Science: Materials in Electronics 27, no. 3: 1-12.
Cristiane Titto; Joao Negrao; Taissa Canaes; Rafael Martins Titto; Thays Mayra Da Cunha Leme-Dos Santos; Fábio Luís Henrique; Raquel Ferrari Calviello; Alfredo Pereira; Evaldo Titto. Heat stress and ACTH administration on cortisol and insulin-like growth factor I (IGF-I) levels in lactating Holstein cows. Journal of Applied Animal Research 2015, 45, 1 -7.
AMA StyleCristiane Titto, Joao Negrao, Taissa Canaes, Rafael Martins Titto, Thays Mayra Da Cunha Leme-Dos Santos, Fábio Luís Henrique, Raquel Ferrari Calviello, Alfredo Pereira, Evaldo Titto. Heat stress and ACTH administration on cortisol and insulin-like growth factor I (IGF-I) levels in lactating Holstein cows. Journal of Applied Animal Research. 2015; 45 (1):1-7.
Chicago/Turabian StyleCristiane Titto; Joao Negrao; Taissa Canaes; Rafael Martins Titto; Thays Mayra Da Cunha Leme-Dos Santos; Fábio Luís Henrique; Raquel Ferrari Calviello; Alfredo Pereira; Evaldo Titto. 2015. "Heat stress and ACTH administration on cortisol and insulin-like growth factor I (IGF-I) levels in lactating Holstein cows." Journal of Applied Animal Research 45, no. 1: 1-7.