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Brisket disease is heritable but is also associated with non-genetic risk factors and effects of the disease on the rumen microbiome are unknown. Ten Holstein heifers were exposed to the plateau environment for three months and divided into two groups according to the index of brisket disease, the mean pulmonary arterial pressure (mPAP): brisket disease group (BD, n = 5, mPAP > 63 mmHg) and healthy heifer group (HH, n = 5, mPAP < 41 mmHg). Rumen fluid was collected for analysis of the concentrations of volatile fatty acids (VFAs). Extracted DNA from rumen contents was analyzed using Illumina MiSeq 16S rRNA sequencing technology. The concentration of total VFA and alpha-diversity metrics were significantly lower in BD group (p < 0.05). Ruminococcus and Treponema were significantly decreased in BD heifers (p < 0.05). Correlation analysis indicated that 10 genera were related to the mPAP (p < 0.05). Genera of Anaerofustis, Campylobacter, and Catonella were negatively correlated with total VFA and acetic acid (R < −0.7, p < 0.05), while genera of Blautia, YRC22, Ruminococcus, and Treponema were positively related to total VFA and acetic acid (R > 0.7; p < 0.05). Our findings may be a useful biomarker in future brisket disease work.
Naren Gaowa; Kevin Panke-Buisse; Shuxiang Wang; Haibo Wang; Zhijun Cao; Yajing Wang; Kun Yao; Shengli Li. Brisket Disease Is Associated with Lower Volatile Fatty Acid Production and Altered Rumen Microbiome in Holstein Heifers. Animals 2020, 10, 1712 .
AMA StyleNaren Gaowa, Kevin Panke-Buisse, Shuxiang Wang, Haibo Wang, Zhijun Cao, Yajing Wang, Kun Yao, Shengli Li. Brisket Disease Is Associated with Lower Volatile Fatty Acid Production and Altered Rumen Microbiome in Holstein Heifers. Animals. 2020; 10 (9):1712.
Chicago/Turabian StyleNaren Gaowa; Kevin Panke-Buisse; Shuxiang Wang; Haibo Wang; Zhijun Cao; Yajing Wang; Kun Yao; Shengli Li. 2020. "Brisket Disease Is Associated with Lower Volatile Fatty Acid Production and Altered Rumen Microbiome in Holstein Heifers." Animals 10, no. 9: 1712.
Plant response to water stress can be modified by the rhizosphere microbial community, but the range of responses across plant genotypes is unclear. We imposed drought conditions on 116 Festuca arundinacea (tall fescue) accessions using a rainout shelter for 46 days, followed by irrigation, to stimulate drought recovery in 24 days. We hypothesized that prolonged water deficit results in a range of phenotypic diversity (i.e., green color index) across tall fescue genotypes that are associated with distinct microbial taxonomic and functional traits impacting plant drought tolerance. Microbial extracellular enzyme activities of chitinase and phenol oxidase (targeting chitin and lignin) increased in rhizospheres of the 20 most drought tolerant genotypes. Lower rates of fungal (dark septate) endophyte root infection were found in roots of the most drought tolerant genotypes. Bacterial 16S rRNA gene and fungal ITS sequencing showed shifts in microbial communities across water deficit conditions prior to drought, during drought, and at drought recovery, but was not patterned by drought tolerance levels of the plant host. The results suggest that taxonomic information from bacterial 16S rRNA gene and fungal ITS sequences provided little indication of microbial composition impacting drought tolerance of the host plant, but instead, microbial extracellular enzyme activities and root fungal infection results revealed patterned responses from drought.
Kevin Panke-Buisse; Liang Cheng; Huijie Gan; Kyle Wickings; Marty Petrovic; Jenny Kao-Kniffin. Root Fungal Endophytes and Microbial Extracellular Enzyme Activities Show Patterned Responses in Tall Fescues under Drought Conditions. Agronomy 2020, 10, 1076 .
AMA StyleKevin Panke-Buisse, Liang Cheng, Huijie Gan, Kyle Wickings, Marty Petrovic, Jenny Kao-Kniffin. Root Fungal Endophytes and Microbial Extracellular Enzyme Activities Show Patterned Responses in Tall Fescues under Drought Conditions. Agronomy. 2020; 10 (8):1076.
Chicago/Turabian StyleKevin Panke-Buisse; Liang Cheng; Huijie Gan; Kyle Wickings; Marty Petrovic; Jenny Kao-Kniffin. 2020. "Root Fungal Endophytes and Microbial Extracellular Enzyme Activities Show Patterned Responses in Tall Fescues under Drought Conditions." Agronomy 10, no. 8: 1076.
Vermicompost application has been shown to promote plant growth, alter the rhizosphere microbiome, and suppress plant pathogens. These beneficial properties are often attributed to the activity of vermicompost-associated microorganisms. However, little is known about the microbial shifts that occur in the rhizosphere after vermicompost application. To better understand the impact of vermicompost treatments on the assembly of rhizosphere bacterial communities, 16S rDNA communities of vermicompost and rhizospheres of each peat- and soil-grown tomatoes were profiled after conventional fertigation, irrigation without additional nutrients, and addition of three different vermicompost-extracts. The full dataset consisted of 412 identified genera, of which 317 remained following stringent quality filtration. Tomato rhizosphere microbiome responses to treatments were complex and unique between peat and soil growth substrates. Direct colonization of vermicompost-origin taxa into rhizospheres was limited, with genera Photobacterium and Luteimonas colonizing peat rhizospheres, genera Truepera, Phenylobacterium, and Lysinibacillus colonizing soil rhizospheres, and genus Pelagibius appearing in both soil and peat rhizospheres. Further patterns of differential abundance and presence/absence between treatments highlight vermicompost-mediated effects on rhizosphere microbiome assembly as an interplay of rhizosphere medium, direct colonization of vermicompost-origin taxa and vermicompost-induced shifts in the rhizosphere microbial community. This exploratory analysis is intended to provide an initial look at 16S community composition of vermicompost and the effects of vermicompost treatment on the rhizosphere microbiome assembly to highlight interactions of potential merit for subsequent investigations.
Juana Munoz-Ucros; Kevin Panke-Buisse; Jamison Robe. Bacterial community composition of vermicompost-treated tomato rhizospheres. PLOS ONE 2020, 15, e0230577 .
AMA StyleJuana Munoz-Ucros, Kevin Panke-Buisse, Jamison Robe. Bacterial community composition of vermicompost-treated tomato rhizospheres. PLOS ONE. 2020; 15 (4):e0230577.
Chicago/Turabian StyleJuana Munoz-Ucros; Kevin Panke-Buisse; Jamison Robe. 2020. "Bacterial community composition of vermicompost-treated tomato rhizospheres." PLOS ONE 15, no. 4: e0230577.
The protein precipitation (PP) of bovine serum albumin (BSA), lysozyme (LYS), and alfalfa leaf protein (ALF) by four procyanidin-rich condensed tannin (CT) samples in both 2-[N-morpholino]ethanesulfonic acid (MES) and a modified Goering-Van Soest (GVS) buffer is described. Purified CT samples examined include Vitis vinifera seed (mean degree of polymerization [mDP] 4.1, 16.5 % galloylated), Tilia sp. flowers (B-type linkages, mDP 5.9), Vaccinium macrocarpon berries (mDP 8.7, 31.7% A-type linkages) and Trifolium pratense flowers (B-type linkages, mDP 12.3) and were characterized by 2D NMR (>90% purity). In general, CTs precipitated ALF >LYS≥BSA. PP in GVS buffer was 1 to 2.25 times greater than in MES buffer (25 °C). The GVS buffer system better reflects the results/conclusions from the literature on the impact mDP, galloylation and A-type linkages have on PP. Determinations of PP using the MES buffer at 37 °C indicated that some of these differences may be attributed to the temperature GVS buffer determinations are conducted. In vitro PP studies using the GVS buffer may offer better guidance when selecting CT-containing forages and amendments for ruminant feeding studies.
Wayne E. Zeller; Laurie A. Reinhardt; Jamison T. Robe; Michael L. Sullivan; Kevin Panke-Buisse. Comparison of Protein Precipitation Ability of Structurally Diverse Procyanidin-Rich Condensed Tannins in Two Buffer Systems. Journal of Agricultural and Food Chemistry 2020, 68, 2016 -2023.
AMA StyleWayne E. Zeller, Laurie A. Reinhardt, Jamison T. Robe, Michael L. Sullivan, Kevin Panke-Buisse. Comparison of Protein Precipitation Ability of Structurally Diverse Procyanidin-Rich Condensed Tannins in Two Buffer Systems. Journal of Agricultural and Food Chemistry. 2020; 68 (7):2016-2023.
Chicago/Turabian StyleWayne E. Zeller; Laurie A. Reinhardt; Jamison T. Robe; Michael L. Sullivan; Kevin Panke-Buisse. 2020. "Comparison of Protein Precipitation Ability of Structurally Diverse Procyanidin-Rich Condensed Tannins in Two Buffer Systems." Journal of Agricultural and Food Chemistry 68, no. 7: 2016-2023.
Dairy farms are predominantly carbon sources, due to high livestock emissions from enteric fermentation and manure. Integrated crop–livestock systems (ICLSs) have the potential to offset these greenhouse gas (GHG) emissions, as recycling products within the farm boundaries is prioritized. Here, we quantify seasonal and annual greenhouse gas budgets of an ICLS dairy farm in Wisconsin USA using satellite remote sensing to estimate vegetation net primary productivity (NPP) and Intergovernmental Panel on Climate Change (IPCC) guidelines to calculate farm emissions. Remotely sensed annual vegetation NPP correlated well with farm harvest NPP (R2 = 0.9). As a whole, the farm was a large carbon sink, owing to natural vegetation carbon sinks and harvest products staying within the farm boundaries. Dairy cows accounted for 80% of all emissions as their feed intake dominated farm feed supply. Manure emissions (15%) were low because manure spreading was frequent throughout the year. In combination with soil conservation practices, ICLS farming provides a sustainable means of producing nutritionally valuable food while contributing to sequestration of atmospheric CO2. Here, we introduce a simple and cost-efficient way to quantify whole-farm GHG budgets, which can be used by farmers to understand their carbon footprint, and therefore may encourage management strategies to improve agricultural sustainability.
Susanne Wiesner; Alison J. Duff; Ankur R. Desai; Kevin Panke-Buisse. Increasing Dairy Sustainability with Integrated Crop–Livestock Farming. Sustainability 2020, 12, 765 .
AMA StyleSusanne Wiesner, Alison J. Duff, Ankur R. Desai, Kevin Panke-Buisse. Increasing Dairy Sustainability with Integrated Crop–Livestock Farming. Sustainability. 2020; 12 (3):765.
Chicago/Turabian StyleSusanne Wiesner; Alison J. Duff; Ankur R. Desai; Kevin Panke-Buisse. 2020. "Increasing Dairy Sustainability with Integrated Crop–Livestock Farming." Sustainability 12, no. 3: 765.
Repeated planting of the same or closely-related crop species often results in negative soil feedbacks, manifested as reduced plant growth. This is commonly attributed to accumulation of plant pathogenic organisms, but there is increasing evidence that other microbes may contribute as well. Since individual members of the bacterial soil microbiome display distinct preferences for specific root exudates, we hypothesize that enrichment of a small subset of bacterial species in the rhizosphere by monocropping will decrease overall diversity and thus negatively influence the performance of the crop. To test this, we examined soil feedbacks for peanut plants inoculated with bacterial suspensions obtained from monocropped and rotated plots in closed cultivation systems. Partial 16S rRNA gene amplicon sequence analysis revealed significant effects of cropping system on the bacterial composition of peanut rhizospheres. When added to peanut seedling rhizospheres, soil suspensions derived from monocropped plots produced a significant reduction in rhizosphere microbiome species richness (number of OTUs). And, bacterial species including Sphingomonas sp., Herbaspirillum sp., and Arthrobacter sp. were enriched in peanut rhizosphere. However, monocropping-derived soil suspension inoculants showed significant deleterious effects on peanut development compared to rotation-derived inoculants. Further bioassays determined that some enriched bacterial strains that were isolated from the monocropping treatment repressed peanut hypocotyl extension. Our results suggest that bacterial composition assembly in peanut rhizosphere in monocropping system especially that enriches particular deleterious bacterial taxa could lead to clear reductions in plant performance even in the absence of disease or signs of pathogenesis.
Xiaogang Li; Kevin Panke-Buisse; Xiaodong Yao; Devin Coleman-Derr; Changfeng Ding; Xingxiang Wang; Honghua Ruan. Peanut plant growth was altered by monocropping-associated microbial enrichment of rhizosphere microbiome. Plant and Soil 2019, 446, 655 -669.
AMA StyleXiaogang Li, Kevin Panke-Buisse, Xiaodong Yao, Devin Coleman-Derr, Changfeng Ding, Xingxiang Wang, Honghua Ruan. Peanut plant growth was altered by monocropping-associated microbial enrichment of rhizosphere microbiome. Plant and Soil. 2019; 446 (1-2):655-669.
Chicago/Turabian StyleXiaogang Li; Kevin Panke-Buisse; Xiaodong Yao; Devin Coleman-Derr; Changfeng Ding; Xingxiang Wang; Honghua Ruan. 2019. "Peanut plant growth was altered by monocropping-associated microbial enrichment of rhizosphere microbiome." Plant and Soil 446, no. 1-2: 655-669.
We describe a method that adds long-read sequencing to a mix of technologies used to assemble a highly complex cattle rumen microbial community, and provide a comparison to short read-based methods. Long-read alignments and Hi-C linkage between contigs support the identification of 188 novel virus-host associations and the determination of phage life cycle states in the rumen microbial community. The long-read assembly also identifies 94 antimicrobial resistance genes, compared to only seven alleles in the short-read assembly. We demonstrate novel techniques that work synergistically to improve characterization of biological features in a highly complex rumen microbial community.
Derek M. Bickhart; Mick Watson; Sergey Koren; Kevin Panke-Buisse; Laura M. Cersosimo; Maximilian O. Press; Curtis P. Van Tassell; Jo Ann S. Van Kessel; Bradd J. Haley; Seon Woo Kim; Cheryl Heiner; Garret Suen; Kiranmayee Bakshy; Ivan Liachko; Shawn T. Sullivan; Phillip R. Myer; Jay Ghurye; Mihai Pop; Paul J. Weimer; Adam M. Phillippy; Timothy P. L. Smith. Assignment of virus and antimicrobial resistance genes to microbial hosts in a complex microbial community by combined long-read assembly and proximity ligation. Genome Biology 2019, 20, 1 -18.
AMA StyleDerek M. Bickhart, Mick Watson, Sergey Koren, Kevin Panke-Buisse, Laura M. Cersosimo, Maximilian O. Press, Curtis P. Van Tassell, Jo Ann S. Van Kessel, Bradd J. Haley, Seon Woo Kim, Cheryl Heiner, Garret Suen, Kiranmayee Bakshy, Ivan Liachko, Shawn T. Sullivan, Phillip R. Myer, Jay Ghurye, Mihai Pop, Paul J. Weimer, Adam M. Phillippy, Timothy P. L. Smith. Assignment of virus and antimicrobial resistance genes to microbial hosts in a complex microbial community by combined long-read assembly and proximity ligation. Genome Biology. 2019; 20 (1):1-18.
Chicago/Turabian StyleDerek M. Bickhart; Mick Watson; Sergey Koren; Kevin Panke-Buisse; Laura M. Cersosimo; Maximilian O. Press; Curtis P. Van Tassell; Jo Ann S. Van Kessel; Bradd J. Haley; Seon Woo Kim; Cheryl Heiner; Garret Suen; Kiranmayee Bakshy; Ivan Liachko; Shawn T. Sullivan; Phillip R. Myer; Jay Ghurye; Mihai Pop; Paul J. Weimer; Adam M. Phillippy; Timothy P. L. Smith. 2019. "Assignment of virus and antimicrobial resistance genes to microbial hosts in a complex microbial community by combined long-read assembly and proximity ligation." Genome Biology 20, no. 1: 1-18.
The characterization of microbial communities by metagenomic approaches has been enhanced by recent improvements in short-read sequencing efficiency and assembly algorithms. We describe the results of adding long-read sequencing to the mix of technologies used to assemble a highly complex cattle rumen microbial community, and compare the assembly to current short read-based methods applied to the same sample. Contigs in the long-read assembly were 7-fold longer on average, and contained 7-fold more complete open reading frames (ORF), than the short read assembly, despite having three-fold lower sequence depth. The linkages between long-read contigs, provided by proximity ligation data, supported identification of 188 novel viral-host associations in the rumen microbial community that suggest cross-species infectivity of specific viral strains. The improved contiguity of the long-read assembly also identified 94 antimicrobial resistance genes, compared to only seven alleles identified in the short-read assembly. Overall, we demonstrate a combination of experimental and computational methods that work synergistically to improve characterization of biological features in a highly complex rumen microbial community.
Derek M. Bickhart; Mick Watson; Sergey Koren; Kevin Panke-Buisse; Laura M. Cersosimo; Maximilian O. Press; Curtis P. Van Tassell; Jo Ann S. Van Kessel; Bradd J. Haley; Seon Woo Kim; Cheryl Heiner; Garret Suen; Kiranmayee Bakshy; Ivan Liachko; Shawn T. Sullivan; Jay Ghurye; Mihai Pop; Paul J. Weimer; Adam M. Phillippy; Timothy P.L. Smith; Maximillian O Press. Assignment of virus and antimicrobial resistance genes to microbial hosts in a complex microbial community by combined long-read assembly and proximity ligation. 2018, 491175 .
AMA StyleDerek M. Bickhart, Mick Watson, Sergey Koren, Kevin Panke-Buisse, Laura M. Cersosimo, Maximilian O. Press, Curtis P. Van Tassell, Jo Ann S. Van Kessel, Bradd J. Haley, Seon Woo Kim, Cheryl Heiner, Garret Suen, Kiranmayee Bakshy, Ivan Liachko, Shawn T. Sullivan, Jay Ghurye, Mihai Pop, Paul J. Weimer, Adam M. Phillippy, Timothy P.L. Smith, Maximillian O Press. Assignment of virus and antimicrobial resistance genes to microbial hosts in a complex microbial community by combined long-read assembly and proximity ligation. . 2018; ():491175.
Chicago/Turabian StyleDerek M. Bickhart; Mick Watson; Sergey Koren; Kevin Panke-Buisse; Laura M. Cersosimo; Maximilian O. Press; Curtis P. Van Tassell; Jo Ann S. Van Kessel; Bradd J. Haley; Seon Woo Kim; Cheryl Heiner; Garret Suen; Kiranmayee Bakshy; Ivan Liachko; Shawn T. Sullivan; Jay Ghurye; Mihai Pop; Paul J. Weimer; Adam M. Phillippy; Timothy P.L. Smith; Maximillian O Press. 2018. "Assignment of virus and antimicrobial resistance genes to microbial hosts in a complex microbial community by combined long-read assembly and proximity ligation." , no. : 491175.
The microbiome of a vineyard may play a critical role in fruit development, and consequently, may impact quality properties of grape and wine. Vineyard management approaches that have directly manipulated the microbiome of grape clusters have been studied, but little is known about how vineyard management practices that impact the soil microbial pool can influence this dynamic. We examined three under-vine soil management practices: 1) herbicide application, 2) soil cultivation (vegetation removal), and 3) natural vegetation (no vegetation removal) in a Riesling vineyard in New York over a three-year period. The microbiomes associated with soil and grapes were profiled using high-throughput sequencing of the bacterial 16 S rRNA gene and fungal ITS regions. Our results showed that soil bacterial composition under natural vegetation differs from that seen in glyphosate-maintained bare soil. Soil fungal composition under the natural vegetation treatment was distinct from other treatments. Although our study revealed soil microbiome shifts based on under-vine management, there were no corresponding changes in fruit-associated microbial composition. These results suggested that other vineyard management practices or environmental factors are more influential in shaping the grape-associated microbiome.
Ming-Yi Chou; Justine Vanden Heuvel; Terrence Bell; Kevin Panke-Buisse; Jenny Kao-Kniffin. Vineyard under-vine floor management alters soil microbial composition, while the fruit microbiome shows no corresponding shifts. Scientific Reports 2018, 8, 1 -9.
AMA StyleMing-Yi Chou, Justine Vanden Heuvel, Terrence Bell, Kevin Panke-Buisse, Jenny Kao-Kniffin. Vineyard under-vine floor management alters soil microbial composition, while the fruit microbiome shows no corresponding shifts. Scientific Reports. 2018; 8 (1):1-9.
Chicago/Turabian StyleMing-Yi Chou; Justine Vanden Heuvel; Terrence Bell; Kevin Panke-Buisse; Jenny Kao-Kniffin. 2018. "Vineyard under-vine floor management alters soil microbial composition, while the fruit microbiome shows no corresponding shifts." Scientific Reports 8, no. 1: 1-9.
Historical changes in population size, such as those caused by demographic range expansions, can produce nonadaptive changes in genomic diversity through mechanisms such as gene surfing. We propose that demographic range expansion of a microbial population capable of horizontal gene exchange can result in genome surfing, a mechanism that can cause widespread increase in the pan-genome frequency of genes acquired by horizontal gene exchange. We explain that patterns of genetic diversity within Streptomyces are consistent with genome surfing, and we describe several predictions for testing this hypothesis both in Streptomyces and in other microorganisms.
Mallory J. Choudoir; Kevin Panke-Buisse; Cheryl P. Andam; Daniel H. Buckley. Genome Surfing As Driver of Microbial Genomic Diversity. Trends in Microbiology 2017, 25, 624 -636.
AMA StyleMallory J. Choudoir, Kevin Panke-Buisse, Cheryl P. Andam, Daniel H. Buckley. Genome Surfing As Driver of Microbial Genomic Diversity. Trends in Microbiology. 2017; 25 (8):624-636.
Chicago/Turabian StyleMallory J. Choudoir; Kevin Panke-Buisse; Cheryl P. Andam; Daniel H. Buckley. 2017. "Genome Surfing As Driver of Microbial Genomic Diversity." Trends in Microbiology 25, no. 8: 624-636.
The collection of microorganisms found in the root zone of soil, termed the rhizosphere microbiome, has been shown to impact plant growth and development. Here, we tease apart the function of the cultivable portion of the microbiome from the whole microbiome in retaining plant traits modified through artificial selection on flowering time. Specifically, the whole microbiome associated with earlier flowering time of Arabidopsis thaliana was cultivated on four types of solid media to create cultivated fractions of the microbiome. These cultivated microbiomes were subsequently preserved in glycerol, frozen, and revived to yield a portion of the cultivable fraction to compare (1) whole microbiome, (2) cultivable microbiome, and (3) revived, cultivable microbiome controls on early flowering time. Plants grown in soils inoculated with bacteria grown on 25 % Luria broth and 10 % tryptic soy agar retained the early flowering trait. An increase in leaf biomass with two of the cultivated microbiomes (49.4 and 38.5 %) contrasted the lowered biomass effect of the whole microbiome. Inoculation with the cultivated microbiomes that were cryopreserved in glycerol showed no effect on flowering time or leaf biomass. The results indicate that the cultivable portion of a plant's microbiome retains the early flowering effect in A. thaliana, but cryopreservation of the cultivated microbiomes disrupts the microbial effects on flowering time. Furthermore, the contrasting effects on leaf biomass (an indirect response from selection on early flowering time), seen with the whole microbiome versus the cultivable portion, suggests versatility in using cultivation methods to modify multiple traits of plants.
Kevin Panke-Buisse; Stacey Lee; Jenny Kao-Kniffin. Cultivated Sub-Populations of Soil Microbiomes Retain Early Flowering Plant Trait. Microbial Ecology 2016, 73, 394 -403.
AMA StyleKevin Panke-Buisse, Stacey Lee, Jenny Kao-Kniffin. Cultivated Sub-Populations of Soil Microbiomes Retain Early Flowering Plant Trait. Microbial Ecology. 2016; 73 (2):394-403.
Chicago/Turabian StyleKevin Panke-Buisse; Stacey Lee; Jenny Kao-Kniffin. 2016. "Cultivated Sub-Populations of Soil Microbiomes Retain Early Flowering Plant Trait." Microbial Ecology 73, no. 2: 394-403.
Plants generally respond to nitrogen (N) fertilization with increased growth, but N addition can also suppress rhizosphere effects, which consequently alters soil processes. We quantified the influence of N addition on rhizosphere effects of two C4 grasses: smooth crabgrass (Digitaria ischaemum) and bermudagrass (Cynodon dactylon). Plants were grown in nutrient-poor soil for 80 days with either 20 or 120 µg NH4NO3-N g dry soil−1. N mineralization rates, microbial biomass, extracellular enzyme activities and bacterial community structure were measured on both rhizosphere and bulk (unplanted) soils after plant harvest. Fertilization showed nominal differences in net N mineralization, extracellular enzyme activity and microbial biomass between the rhizosphere and bulk soils, indicating minimal influence of N on rhizosphere effects. Instead, the presence of plant roots showed the strongest impact (up to 80%) on rates of net N mineralization and activities of three soil enzymes indicative of N release from organic matter. Principal component analysis of terminal restriction fragment length polymorphism (T-RFLP) also reflected these trends by highlighting the importance of plant roots in structuring the soil bacterial community, followed by plant species and N fertilization (to a minor extent). Overall, the results indicate minor contributions of short-term N fertilization to changes in the magnitude of rhizosphere effects for both grass species.
Biao Zhu; Kevin Panke-Buisse; Jenny Kao-Kniffin. Nitrogen fertilization has minimal influence on rhizosphere effects of smooth crabgrass (Digitaria ischaemum) and bermudagrass (Cynodon dactylon). Journal of Plant Ecology 2014, 8, 390 -400.
AMA StyleBiao Zhu, Kevin Panke-Buisse, Jenny Kao-Kniffin. Nitrogen fertilization has minimal influence on rhizosphere effects of smooth crabgrass (Digitaria ischaemum) and bermudagrass (Cynodon dactylon). Journal of Plant Ecology. 2014; 8 (4):390-400.
Chicago/Turabian StyleBiao Zhu; Kevin Panke-Buisse; Jenny Kao-Kniffin. 2014. "Nitrogen fertilization has minimal influence on rhizosphere effects of smooth crabgrass (Digitaria ischaemum) and bermudagrass (Cynodon dactylon)." Journal of Plant Ecology 8, no. 4: 390-400.
Soil microorganisms found in the root zone impact plant growth and development, but the potential to harness these benefits is hampered by the sheer abundance and diversity of the players influencing desirable plant traits. Here, we report a high level of reproducibility of soil microbiomes in altering plant flowering time and soil functions when partnered within and between plant hosts. We used a multi-generation experimental system using Arabidopsis thaliana Col to select for soil microbiomes inducing earlier or later flowering times of their hosts. We then inoculated the selected microbiomes from the tenth generation of plantings into the soils of three additional A. thaliana genotypes (Ler, Be, RLD) and a related crucifer (Brassica rapa). With the exception of Ler, all other plant hosts showed a shift in flowering time corresponding with the inoculation of early- or late-flowering microbiomes. Analysis of the soil microbial community using 16 S rRNA gene sequencing showed distinct microbiota profiles assembling by flowering time treatment. Plant hosts grown with the late-flowering-associated microbiomes showed consequent increases in inflorescence biomass for three A. thaliana genotypes and an increase in total biomass for B. rapa. The increase in biomass was correlated with two- to five-fold enhancement of microbial extracellular enzyme activities associated with nitrogen mineralization in soils. The reproducibility of the flowering phenotype across plant hosts suggests that microbiomes can be selected to modify plant traits and coordinate changes in soil resource pools.
Kevin Panke-Buisse; Angela Poole; Julia K Goodrich; Ruth Ley; Jenny Kao-Kniffin. Selection on soil microbiomes reveals reproducible impacts on plant function. The ISME Journal 2014, 9, 980 -989.
AMA StyleKevin Panke-Buisse, Angela Poole, Julia K Goodrich, Ruth Ley, Jenny Kao-Kniffin. Selection on soil microbiomes reveals reproducible impacts on plant function. The ISME Journal. 2014; 9 (4):980-989.
Chicago/Turabian StyleKevin Panke-Buisse; Angela Poole; Julia K Goodrich; Ruth Ley; Jenny Kao-Kniffin. 2014. "Selection on soil microbiomes reveals reproducible impacts on plant function." The ISME Journal 9, no. 4: 980-989.